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Citations for LI-COR :
601 - 650 of 676 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were transferred to nitrocellulose (for fluorescence detection) or PVDF (for chemiluminescent detection) membranes and blocked with Odyssey Blocking Buffer (LI-COR) or 5% milk ...
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bioRxiv - Immunology 2021Quote: ... the slides were washed with 1 × PBS for 10□min and blocked with Odyssey Blocking Buffer (LI-COR Biosciences, # 927-40000) for 1□h ...
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bioRxiv - Cancer Biology 2021Quote: ... The blots were subsequently blocked for 1 h at room temperature with Intercept® (TBS) blocking buffer (LI-COR, # 927-60001). The blots were finally probed ...
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bioRxiv - Microbiology 2020Quote: ... whole cell lysates were mixed with 4x or 6x Protein Sample Loading Buffer (LI-COR, at a final dilution of 1x) supplemented with 10 % β-mercaptoethanol (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies (IRDye 800CW Donkey anti-Rabbit IgG Secondary Antibody) were used at 1:5000 in Odyssey blocking buffer (LI-COR) for one hour at room temperature ...
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bioRxiv - Systems Biology 2022Quote: ... blotted to polyvinylidene difluoride membrane by wet-tank transfer for 1.5 h at 100V constant voltage and blocked with intercept (TBS) blocking buffer (LI-COR Biosciences) for 1 h.
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bioRxiv - Immunology 2022Quote: Denature of Protein of both the cytoplasmic extract and nuclear extract was performed utilizing 4X Protein Sample Loading Buffer (LI-COR) with β-mercaptoethanol ...
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bioRxiv - Cancer Biology 2022Quote: ... using a wet transfer apparatus and blocked using either LI-COR Intercept (TBS) Blocking Buffer (LI-COR, Cat. No. 927-60001) or 5% milk in TBST (20mM Tris pH 7.5,150mM NaCl with 0.1% Tween-20) ...
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bioRxiv - Cancer Biology 2021Quote: ... Blots were washed 3X with 1% TBST buffer and probed with secondary antibodies 680RD goat anti-Mouse and 800CW goat anti-Rabbit (LI-COR) for 1hr ...
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bioRxiv - Immunology 2020Quote: ... Proteins were transferred to a polyvinylidene fluoride (PVDF) membrane and blocked for 1 hour in PBST with 50% Odyssey Blocking Buffer (LI-COR). Primary antibodies were incubated overnight in the same blocking solution ...
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bioRxiv - Biochemistry 2022Quote: ... and then incubated for 1 hr at room temperature with LI-COR secondary antibodies diluted in Odyssey Blocking Buffer containing 0.2% Tween-20: IRDye 800CW goat anti-mouse IgG (LI-COR, AB_621842) and IRDye 680RD goat anti-rabbit IgG (LI-COR ...
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bioRxiv - Neuroscience 2022Quote: ... atTubulin serves as a loading control 1:20,000 in either 5%milk in TBST or Odyssey Blocking Buffer in PBS (LI-COR Biosciences) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... IRDye secondary antibody (800CW anti-rabbit IgG (#925-32213, 1:10000) and Odyssey blocking PBS buffer (#92740000) were from LI-COR Biosciences (Lincoln ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were probed overnight at 4°C with the primary antibodies diluted at 1:1000 in Intercept TBS blocking buffer (LI-COR). Blots were washed at least three times in 1x TBS containing 0.1% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... The blot was then washed three times with 1X TBST buffer at room temperature and probed with 1:10000 dilutions of mouse secondary antibody (LI-COR Biosciences IRDye® 680RD Goat anti-mouse IgG ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins were transferred to a PVDF membrane which was then blocked with tris-buffered saline-based Odyssey blocking buffer (LI-COR) for 1 hr at room temperature (RT ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed 3 times with Wash Buffer and signals were obtained by using the Odyssey Infrared Imaging System (LI-COR).
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bioRxiv - Neuroscience 2023Quote: ... and vessel samples (20µg) were diluted in Tris-glycine SDS sample buffer and the equally diluted samples and fluorescent standards (LI-COR Biosciences) were loaded onto Novex 10% zymogram plus gelatin protein gels (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was washed with PBS-T (3 x Sm) and incubated in secondary antibody (Table 53) diluted in Intercept Blocking Buffer (LI-COR) for 1 hour at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were de-stained then blocked for 1 hour at room temperature using INTERCEPT (TBS) Blocking Buffer (LI-COR #926-11021). Blots were incubated in primary antibodies diluted in INTERCEPT (TBS ...
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bioRxiv - Microbiology 2024Quote: ... Membranes probed for YAP1 or with one of several phospho-specific antibodies (YAP1 pS127, LATS1 pT1079, NF2 pS518) were blocked with TBS-based Intercept Blocking Buffer (LI-COR) and incubated with antibodies diluted in TBS-based Intercept Antibody Diluent (LI-COR) ...
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bioRxiv - Systems Biology 2024Quote: ... 1:10000 dilution in Intercept Blocking Buffer) were used as the secondary antibody and were visualized on the Odyssey M Imaging System (LI-COR). Signal intensities were quantified from the profile plot of each lane using ImageJ (Rasband ...
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bioRxiv - Neuroscience 2024Quote: ... The NC membrane was fixed in 70% methanol for 20 minutes under agitation and blocked using Odyssey blocking buffer (LI-COR), then incubated overnight with primary antibodies diluted in Odyssey blocking buffer and 0.2% Tween20 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies were diluted 1:7500 in a mixture of 50% v/v of Intercept blocking buffer (LI-COR, 927-60001) in TBS-0.1%Tween20 and incubated for 2 hours at room temperature in the dark ...
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bioRxiv - Bioengineering 2024Quote: ... the membranes were incubated in blocking buffer with IRDye® 800CW and 680CW goat IgG secondary antibodies (1:10,000) from LI-COR for 2 hours and the membranes were imaged using the Bio-Rad ChemiDoc MP imaging system.
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bioRxiv - Cell Biology 2024Quote: ... membranes were blotted with the appropriate primary antibody (Suppl. Table 3) and infrared dyed secondary antibodies (Suppl. Table 4) diluted in Odyssey Blocking Buffer (LI-COR) supplemented with 0.1% Tween-20 and 0.01% Sodium Azide ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR products were run on a 6% acrylamide gel on a LI-COR 4300 DNA Analyzer (LI-COR Inc., Lincoln, NE, USA). To maintain consistency of scoring alleles ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was incubated at room temperature for 30 min before mixing with protein sample loading buffer (LI-COR Biosciences, 928-40004).
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was blocked in 5% milk in PBS-T (0.05% Tween-20) or Odyssey Blocking Buffer (for IR-Dye detection; LI-COR 927-40000) and probed with primary antibodies ...
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bioRxiv - Genetics 2019Quote: ... Western blotting was performed using LI-COR buffer and species appropriate secondary antibodies conjugated to IR-dye700 or IRdye-800 (LI-COR Biosciences), according to the manufacturer’s guidelines and analyzed using LI-COR Odyssey ...
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bioRxiv - Developmental Biology 2020Quote: ... Reactions were stopped with non-reducing SDS loading buffer and analysed via immunoblotting and membranes scanned in an Odyssey CLx Infrared Imaging System (LI-COR Biosciences). Protein signals were quantified using Image Studio software (LI-COR Biosciences) ...
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bioRxiv - Cell Biology 2020Quote: ... supernatants were collected and protein concentration was determined using a BCA assay (PIERCE) prior to western blot analysis using the Odyssey blocking buffers (LI-COR Biosciences). Blots were developed using the Odyssey Infrared Imaging System (LI-COR Biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... The blots were washed and blocked for 1 h in 5% dry milk or Intercept (TBS) blocking buffer (LI-COR, 927-60001) and incubated with primary antibodies overnight at 4°C (diluted in 1x TBS with 0.1% Tween® 20) ...
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bioRxiv - Biochemistry 2021Quote: ... the cells were permeabilised using ice-cold methanol (10 min, room temperature) and then blocked using Odyssey Blocking Buffer in TBS (LI-COR Biosciences) (1.5h ...
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bioRxiv - Microbiology 2021Quote: ... Blocking of membranes was carried out by incubation with either a 5% BSA solution or the proprietary Odyssey Blocking Buffer (LI-COR Biosciences) for one hour ...
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bioRxiv - Cell Biology 2021Quote: ... protein-free blocking buffer supplemented with 0.2% tween-20 was used for all blocking and antibody staining steps (LI-COR, #927-70001). For microtubules specific fractionation we used a microtubules/tubulin in vivo assay kit (Cytoskeleton ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were blocked for approximately one hour in either a 1:1 solution of TBS (20 mM Tris, 500 mM NaCl [pH 7.4]) and Odyssey Blocking Buffer (LI-COR, 927-70001) or PBS with 1% BSA ...
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bioRxiv - Physiology 2021Quote: ... This was followed by an incubation of primary antibodies overnight at 4°C in 20 ml of Odyssey Blocking Buffer (LI-COR Biosciences), and 20 µl of Tween-20 (Sigma-Aldrich ...
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bioRxiv - Genomics 2021Quote: ... the gels were transferred onto nitrocellulose membranes and the membranes were blocked by Odyssey Blocking Buffer (PBS) (LI-COR, Cat#927-50000), followed by overnight incubation with primary antibodies at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... they were washed and permeabilized in washing buffer (0.2% Tween 20 in PBS) 3 times for 5 minutes each and incubated for 60 minutes in blocking buffer (PBS version; LI-COR Biosciences). Fixed cells were then incubated for 1 hour each with primary and secondary antibodies diluted into blocking buffer supplemented with Tween 20 to 0.2% ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were incubated at room temperature for 90 minutes in a 1:1 blocking solution of 1X PBS and PBS Odyssey® Blocking Buffer (cat. no. 927-4000, LI-COR) or PBS Intercept® Blocking Buffer (cat ...
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bioRxiv - Cancer Biology 2022Quote: ... An 8% Acrylamide SDS-PAGE was used to separate out the protein and nitrocellulose membranes were blocked in Odyssey TBS blocking buffer (LI-COR Biosciences) for at least 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were cut to size and blocked for at least 30 minutes at room temperature in Intercept TBS Blocking buffer (LI-COR Biosciences) on a shaker ...
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bioRxiv - Physiology 2023Quote: ... Conditioned media samples were diluted in Tris-glycine SDS sample buffer and the equally diluted samples and fluorescent standards (LI-COR Biosciences) were loaded onto Novex 10% zymogram plus gelatin protein gels (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked for 1 h at room temperature with either 5% milk in Tris-buffered saline plus Tween (TBST) or Odyssey Blocking Buffer in phosphate-buffered saline (PBS) (LI-COR Biosciences) and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... The membranes were washed 3 times with PBST buffer for 10 minutes and incubated with secondary antibodies (PBST buffer + 5% BSA + 0.1μg/mL of both goat anti-rabbit 800nm (LI-COR Biosciences; #925-32211) dilution 1:10,000 and goat anti-mouse 680nm antibodies dilution 1:10,000 (LI-COR Biosciences ...
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bioRxiv - Biophysics 2022Quote: ... the membranes were washed 3 times for 10 min with PBST buffer before imaging using the LI-COR Odyssey XF (LI-COR Biosciences).
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bioRxiv - Cell Biology 2023Quote: ... Primary cleaved-CASP3 antibody was diluted 1:500 in 50% PBS-T: 50% Odyssey Blocking Buffer (OBB, LI-COR Odyssey, #927-40010). Samples were then incubated with diluted cleaved-CASP3 antibody at room temperature for 8 hours ...
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bioRxiv - Biophysics 2023Quote: ... incubated with a 1/3000 dilution of goat anti-rabbit secondary antibody in blocking buffer conjugated to IRDye® 680RD (LI-COR), washed ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were again washed 3 times with Wash Buffer and incubated with IRDye 800CW Streptavidin (1:2000; LI-COR, Lincoln, NE, USA) for 30 min at room temperature ...