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Citations for Genesee Scientific :
1 - 50 of 68 citations for rno mir 134 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... SYBR Green based real-time PCR was performed using Apex qPCR Master Mix (Genesee Scientific). PCR conditions were as follows ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Real-time Taqman-PCR was assembled in qPCRBIO Probe Blue Mix (Genesee Scientific Corporation, 17-514) or TaqMan Fast Advanced Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time Taqman-PCR was assembled in qPCRBIO Probe Blue Mix (Genesee Scientific Corporation, 17-514) with 0.5μM of each primer (Forward ...
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bioRxiv - Cell Biology 2023Quote: ... Embryos were staged in Halocarbon 27 oil (Genesee Scientific, #59-134) and those of the relevant stage were dechorionated in 50% bleach solution ...
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bioRxiv - Cell Biology 2020Quote: SYBRGreen based real-time PCR was performed using Apex qPCR GREEN Master Mix (Genesee Scientific, San Diego, CA, USA). The experiments were performed as triplicates of at least three independent experiments and are expressed as a ~fold change compared to the control ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were visualized using ProSignal Pico ECL Western Blotting detection kit (Genesee Scientific).
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bioRxiv - Biochemistry 2022Quote: ... Proteins were then visualized using the ProSignal Pico ECL Western Blotting detection kit (Genesee Scientific).
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bioRxiv - Neuroscience 2019Quote: ... blots were visualized using an Amersham ECL Western Blotting Detection Kit (GE) or the SuperSignal West Femto Maximum Sensitivity Substrate (Thermo) and developed on X-ray film (Genesee Scientific). The following primary antibodies were used ...
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bioRxiv - Neuroscience 2024Quote: ... Detection was performed using enhanced chemiluminescence substrate (Genesee Scientific, 20– 300S) and imaged on a ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... sgRNA PCRs were pooled and purified with a Zymo Research DNA Clean and Concentrator kit (Genesee Scientific) and resuspended in 25 μL of Ultra-Pure Water (Genesee) ...
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bioRxiv - Microbiology 2023Quote: ... using the ProSignal Pico ECL Reagent detection reagent (Genesee Scientific, El Cajon, CA).
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bioRxiv - Microbiology 2023Quote: ... The PCR product was gel purified from a 1.5% Agarose TAE gel using the Zymoclean Gel Extraction Kit (Genesee Scientific). This fragment was then built together with other parts of the pUCP18-GHD vector to obtain the pNLuc10-popD plasmid ...
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bioRxiv - Bioengineering 2022Quote: ... RT-qPCR was performed with SYBR green (qPCRBIO SyGreen Blue Mix Separate-ROX Cat #: 17-507B, Genesee Scientific). 4 μl of diluted cDNA was used for each 20 μl reaction containing a final primer concentration of 200 nM and 10 μl of SYBR green buffer solution ...
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bioRxiv - Bioengineering 2023Quote: ... RT-qPCR was performed with SYBR green (qPCRBIO SyGreen Blue Mix Separate-ROX Cat #: 17-507B, Genesee Scientific), using 4 μl of diluted cDNA for each 20 μl reaction containing a final primer concentration of 200 nM and 10 μl of SYBR green buffer solution ...
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bioRxiv - Systems Biology 2024Quote: ... Membranes were blocked 60 minutes at RT in OneBlock™ blocking buffer (#20-314, Genesee Scientific, Morrisville, NC) followed by overnight incubation at 4°C in primary antibodies for ITGAV (1:1,000 ...
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bioRxiv - Developmental Biology 2023Quote: ... The generated cDNA was diluted 2x and 1µL was used for PCR amplification using PCR Biosystems VeriFi Mix (Genesee Scientific, 17-308B). A touchdown-PCR method was used for amplification ...
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bioRxiv - Genetics 2024Quote: ... Each PCR reaction contained Sybr Green 2X Master Mix (Genesee Scientific), appropriate primer pairs (Figure 1A ...
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bioRxiv - Pathology 2019Quote: ... at which time food media was switched to 0.5g Genesee Scientific instant fly media (Genesee Scientific #66-117) hydrated with 2ml of water containing vehicle control (150 ul ethanol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... with 12.5μl Apex PCR Master Mix (Genesee Scientific, San Diego, California, USA), nine μl pure water ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.5 µL of dNTPs (17-106, PCR Biosystems, Genesee Scientific, El Cajon, CA, USA), 0.75 µL of MgCl2 (42-800B3 ...
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bioRxiv - Genomics 2022Quote: ... Each 50 μL reaction contained 5 μL of 10X PCR buffer (Genesee, SanDiego, CA), 2.5 μL dNTPs (2 mM per nucleotide) ...
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bioRxiv - Cell Biology 2023Quote: ... cartilage was washed three times in phosphate buffered saline (PBS; potassium phosphate, sodium chloride, sodium phosphate dibasic; GenClone; Genesee Scientific, San Diego, CA, USA) and then placed in 0.1% collagenase (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... and stored at -20°C in a sealed 96-well PCR plate (Genesee Scientific, cat#24-302).
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bioRxiv - Genomics 2021Quote: ... reactions were set up in 10 μl volumes with: 5 μl 2×Apex PCR Master Mix (Genesee Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Zymo Research Kit (Genesee: 11-328). RNA concentration was measured using the Nanodrop1000 spectrophotometer (Thermo Fisher) ...
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bioRxiv - Biochemistry 2023Quote: ... Bulk washed resin could be stored in water at room temperature for several weeks before deployment at which time 100 g was packaged between two layers of Nitex™ nylon mesh (120 µm, Genesee Scientific, El Cajon, CA) and framed within a 30 cm wooden embroidery hoop (Fig ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was subjected to PCR analysis with gene-specific primers in the presence of SyGreen Blue Mix (Genesee). Relative mRNA abundance was obtained by normalization to actin and tubulin housekeeping genes.
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bioRxiv - Neuroscience 2021Quote: ... Direct-zol™ RNA MiniPrep Kit (Genesee Scientific/Zymo Research ...
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bioRxiv - Cancer Biology 2023Quote: ... we performed qRT-PCR of the cDNA using qPCRBIO SyGreen Blue Mix Hi-ROX (Genesee Scientific; Cat #17-506C) and the Applied Biosystems® ViiATM 7 Real-Time PCR System with 384-well Block (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Colonies of potential recombinant mutants were screened using colony PCR with 2.0 X Taq RED Master Mix (Genesee Scientific, USA). For colony PCR ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR reaction reagents included 2.5 µL of 10x Buffer (42-800B3, Apex Bioresearch Products, Genesee Scientific, El Cajon, CA, USA), 0.5 µL of dNTPs (17-106 ...
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bioRxiv - Developmental Biology 2019Quote: ... or Direct-zol RNA Miniprep Kit (Genesee Scientific), and RNA was reverse-transcribed by Superscript IV (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... and purified using Zymo ChIP DNA Concentrator kit (Genesee Scientific #11-379C ...
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bioRxiv - Neuroscience 2023Quote: Small scale odor exposure for downstream qRT-PCR analysis utilized Drosophila food vials (Catalog #32-117BF, Genesee Scientific, San Diego,CA, USA). Food vials with a cottonball (Catalog # 22-456-883 ...
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bioRxiv - Microbiology 2021Quote: ... RNA was isolated using the RNeasy kit (Genesee Scientific, USA) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Zymo DNA clean and concentrator kit (D4004) was from Genesee Scientific ...
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bioRxiv - Genomics 2022Quote: ... We used 2μL of the resulting DNA mixture in a PCR reaction with primers (Left: 5’-CTAGCACGGAACCCTGGAAAT -3’; Right: 5’-GCAGCGCCTAGTAATCACAGA -3’) according to ApexRedTaq (Genesee Scientific, El Cajon, CA) manufacturer instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmid DNA was purified using Zyppy Plasmid Miniprep Kit (Genesee Scientific) and sequenced using primers as followed ...
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bioRxiv - Microbiology 2022Quote: ... and genomic DNA was extracted using the Quick-DNA Miniprep Kit (Genesee) or the DNeasy Blood & Tissue Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was isolated using Quick-RNA MiniPrep Kit (Genesee Scientific: 11-328). When necessary ...
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bioRxiv - Biochemistry 2019Quote: ... and gel-purified with ZymoClean Gel DNA Recovery Kit (Genesee Scientific, #11-300C) to produce the final library ...
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bioRxiv - Immunology 2020Quote: ... 8 and 17 hours by using Direct-zol RNA MiniPrep Kit (Genesee Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and the supernatant processed by the Direct-zol RNA MiniPrep kit (Genesee Scientific) per the manufacturer’s instructions with an additional dry spin after disposing of the final wash to prevent EtOH carryover ...
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bioRxiv - Immunology 2023Quote: ... Total protein concentrations were determined using BCA protein assay kit (Genesee Scientific, Cat# 18-440). Proteins were separated using SDS-PAGE electrophoresis and then transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Biochemistry 2024Quote: ... the modified RNA was purified using the RNA Clean & Concentrator-5 kit (Genesee Scientific #R1014). The RNA sample was eluted from the column with 7 μL of RNase-Free water ...
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bioRxiv - Biochemistry 2024Quote: ... The DNA was then purified using the Zymoclean Gel DNA Recovery Kit (Genesee Scientific #11-301C). Lastly ...
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bioRxiv - Biochemistry 2024Quote: ... the cDNA product was purified using the Oligo Clean and Concentrator Kit (Genesee Scientific #11-380B). Before starting the purification ...
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bioRxiv - Genetics 2022Quote: Genomic DNA was isolated from yeast strains using the Zymo YeastStar genomic DNA isolation kit (Genesee Scientific) using the manufacturer’s recommended protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... gDNA was extracted using the YeaStar Genomic DNA Kit (Genesee Scientific, San Diego, CA, Cat #11-323) according to the manufacturer’s “Protocol 1” ...
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bioRxiv - Microbiology 2023Quote: ... pooled and purified with a Zymo Research DNA Clean and Concentrator kit (Genesee Scientific, San Diego, CA) and resuspended in 25 μL of ultra-pure water (Genesee).