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Citations for Genesee Scientific :
1 - 50 of 80 citations for Mouse DTW Domain Containing Protein 2 DTWD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Total protein concentrations were determined using BCA protein assay kit (Genesee Scientific, Cat# 18-440). Proteins were separated using SDS-PAGE electrophoresis and then transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were visualized using ProSignal Pico ECL Western Blotting detection kit (Genesee Scientific).
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bioRxiv - Biochemistry 2022Quote: ... Proteins were then visualized using the ProSignal Pico ECL Western Blotting detection kit (Genesee Scientific).
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bioRxiv - Immunology 2023Quote: The BRAFV600E PTEN-/- melanoma cell line was generated by spontaneous immortalization of a tumor resected from the BRAFCA;PTENlox/lox;Tyr::CreERT2 mouse model (13) and cultured in DMEM containing 10% FBS (Genesee Scientific, Cat# 25-550) and 1% antibiotic-antimycotic (ThermoFisher Cat ...
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bioRxiv - Cell Biology 2022Quote: ... containing 5% milk (Genesee, 20-241), then incubated with 1:5000 anti-VgrG in 5% milk in TBS-T overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... to select for plasmid-containing cells and carbenicillin (Genesee Scientific), amoxicillin (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... 2% peptone (20-260, Genesee Scientific), and 4% dextrose ...
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bioRxiv - Cell Biology 2022Quote: ... and then quantified using a bicinchoninic acid protein assay (BCA) (Prometheus Protein Biology Products; Genesee Scientific, San Diego, CA, USA). Specific protein levels were determined using a WES Capillary-Based Electrophoresis assay with a 12-230 kDa separation module kit following the manufacturer instructions (Protein Simple ...
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bioRxiv - Bioengineering 2022Quote: ... 2 mM L-glutamine (Genesee catalog #25-509) and 1x MEM non-essential amino acids (Genesee catalog #25-536) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 2 mM L-glutamine (Genesee, catalog# 25-509) and 1X MEM non-essential amino acids (Genesee ...
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bioRxiv - Physiology 2022Quote: ... Protein was transferred to a nitrocellulose membrane (Genesee, 84-876) at 100V for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... sonicated (Model Q55, Qsonica; Newton, CT) and quantified using a bicinchoninic acid (BCA) protein assay (Prometheus Protein Biology Products; Genesee Scientific, San Diego, CA, USA). Protein levels were quantified using WES capillary electrophoresis (Protein Simple ...
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bioRxiv - Neuroscience 2019Quote: ... Experiments were conducted on 2% agarose (Genesee, 20-102). In the experiments described in Figure 2 ...
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bioRxiv - Synthetic Biology 2024Quote: ... 2 mM l-glutamine (Genesee, catalog no. 25-509) and 1× MEM non-essential amino acids (Genesee ...
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bioRxiv - Molecular Biology 2019Quote: ... separated protein products were transferred to nitrocellulose filters (0.45 μm, Genesee Scientific) using 25 mM Tris ...
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bioRxiv - Neuroscience 2020Quote: ... we quickly placed the tubes containing the mosquitoes on a CO2 Flypad (Genesee Scientific; cat. # 59-119), so that the hole was directly pressed up against the pad.
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bioRxiv - Neuroscience 2020Quote: ... Tissue was transferred to a sterile tube containing 300 μl TRI-reagent (Cat.# 11-330T, Genesee Scientific) and homogenized with a mechanical pestle before centrifuging at 13,000 x g for 3 minutes ...
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bioRxiv - Physiology 2022Quote: ... Immunoblotted proteins were detected with ProSignal Pico ECL Reagent (Genesee Scientific, #20-300) and imaged using ChemiDoc MP Imaging System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... Total protein in the lysates were analyzed by BCA assay (Genesee Scientific, 18-440) and normalized before use in the ELISA assays as described.
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bioRxiv - Microbiology 2019Quote: ... groups of at least 40 flies were injected and kept in vials containing cornmeal food (Genesee Scientific #66-112). DGRP survival curves were repeated to verify reproducibility ...
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bioRxiv - Cell Biology 2019Quote: ... and 2 × 105 recipient cells/well (A549) in 6-well plates (Genesee Scientific) were reverse transfected with 5 nM siRNA using Lipofectamine RNAiMAX (ThermoFisher) ...
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bioRxiv - Pathology 2023Quote: ... 1-2 day old adults were placed in standard plastic vials (Genesee Scientific), not more than 35 flies/vial ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were revealed by using ProSignal® Dura ECL Reagent (Genesee Scientific, San Diego, CA) and visualized in a Chemidoc™ Touch Imaging System (Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... with the exception that proteins were transferred to nitrocellulose membranes (Genesee Scientific, Prometheus #84-875). Primary antibodies were used at the following dilutions ...
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bioRxiv - Molecular Biology 2024Quote: ... Zymo Research Kit (Genesee: 11-328). RNA concentration was measured using the Nanodrop1000 spectrophotometer (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein bands were visualized by using ProSignal® Dura ECL Reagent (Genesee Scientific, San Diego, CA) and Chemidoc™ Touch Imaging System (Bio-Rad).
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bioRxiv - Physiology 2019Quote: ... at which time mated females were transferred into egg laying chambers that consisted of a 100ml plastic beaker with holes for air exchange affixed over a petri dish containing grape juice agar (Genesee Scientific). A dollop of yeast paste (active dry yeast mixed with water ...
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bioRxiv - Cell Biology 2020Quote: ... and supernatant was added to 2 70% confluent T-75 flasks (Genesee Scientific, 25-209). Propagation continued and collection repeated for infection of 10 15 cm dishes ...
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bioRxiv - Immunology 2020Quote: All immunoprecipitation experiments were conducted in 2 ml deep-well polypropylene plates (Genesee Scientific, Inc). Plates were blocked with 3% BSA in TBST overnight with overhead rotation on a Rotator Genie prior to use for immunoprecipitations ...
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bioRxiv - Biophysics 2023Quote: ... Culture medium containing virus was collected 48-60 h after transfection and filtered through 0.45 µm syringe filter (Genesee Scientific, 25-245). The target cells (mEos2-ULK1 HeLa ...
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bioRxiv - Cell Biology 2023Quote: ... 270 mg total protein was divided evenly into 11 5-mL centrifuge tubes (Genesee Scientific, 24-285) and rotated with FLAG antibody overnight at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Direct-zol™ RNA MiniPrep Kit (Genesee Scientific/Zymo Research ...
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bioRxiv - Genomics 2021Quote: ... The animals were reared at 25°C on standard fly food containing 14 g inactive dry yeast (Genesee Scientific, Cat. Num. 62-108), 46 g yellow corn meal (VWR ...
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bioRxiv - Developmental Biology 2019Quote: ... or Direct-zol RNA Miniprep Kit (Genesee Scientific), and RNA was reverse-transcribed by Superscript IV (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... Flies were expanded in vials (23 mm X 95 mm) containing ∼ 5 mL of a standard cornmeal-based medium for larval growth (Genesee Scientific, Cat#: 66-113) adapted from the Bloomington Drosophila Stock Center (https://bdsc.indiana.edu) ...
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bioRxiv - Genomics 2021Quote: ... reactions were set up in 10 μl volumes with: 5 μl 2×Apex PCR Master Mix (Genesee Scientific ...
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bioRxiv - Microbiology 2023Quote: ... for 2 h and then filtered using a vacuum-driven 0.22 µm filter (Olympus plastics, Genesee Scientific) prior to the inoculation of cells to remove any remaining trace of metal ...
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bioRxiv - Cancer Biology 2022Quote: ... and purified using Zymo ChIP DNA Concentrator kit (Genesee Scientific #11-379C ...
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bioRxiv - Immunology 2023Quote: ... the protein of interest on the membranes was visualized using ProSignal® Femto ECL Reagent (Genesee Scientific, Cat# 20-302), and the images were acquired using KwikQuant Imager (Kindle Biosciences ...
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bioRxiv - Cell Biology 2023Quote: All the protein samples were separated by sodium dodecyl sulfate polyacrylamide (SDS)-PAGE and were transferred to nitrocellulose membrane (Bio-Rad laboratories, Genesee Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining 2 ml was transferred to a 5 ml polystyrene round bottomed tube (Genesee Scientific, San Diego, CA) and gently combined with 100 μl of isolation cocktail and 100 μl of RapidSpheresTM (Stem Cell Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... RNA was isolated using the RNeasy kit (Genesee Scientific, USA) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Zymo DNA clean and concentrator kit (D4004) was from Genesee Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... and mouse N2a cells (ATCC CCL-131) were cultured in in Dulbecco’s Modified Eagle Media (DMEM, Genesee Scientific Cat. #: 25-500) supplemented with 10% Fetal Bovine Serum (Gibco Cat ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmid DNA was purified using Zyppy Plasmid Miniprep Kit (Genesee Scientific) and sequenced using primers as followed ...
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bioRxiv - Microbiology 2022Quote: Serum samples were serially diluted in RPMI-1640 with 10 mM HEPES and 2% FetalPure bovine serum (Genesee Scientific 25-525H), hereafter referred to as viral diluent ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... or pooled mammary glands from Balb/cJ mice were harvested and chopped into fine pieces using a razor blade before addition to digestion buffer (DMEM/F12 #SH30023, HyClone;1% PenStrep, Gibco - Thermo Fisher; 2% FBS, Genesee Scientific ...
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bioRxiv - Microbiology 2022Quote: ... and genomic DNA was extracted using the Quick-DNA Miniprep Kit (Genesee) or the DNeasy Blood & Tissue Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was isolated using Quick-RNA MiniPrep Kit (Genesee Scientific: 11-328). When necessary ...
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bioRxiv - Cell Biology 2020Quote: sgRNA transduced cells seeded on 6-well plates at ~60% confluency were transfected with 2 μg flag-tagged β2-AR for 48 hr using JetPrime Transfection reagent (Genesee, Cat #55134) following manufacturer’s protocols ...