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Citations for Genesee Scientific :
51 - 92 of 92 citations for 7 PHENYL 1 2 4 TRIAZOLO 1 5 A PYRIMIDINE 6 CARBONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... NIH 3T3s were transfected at 50–75% confluence in 6-well tissue culture plates (25-105; Genesee Scientific, El Cajon, CA) using Lipofectamine 2000 (11668019 ...
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bioRxiv - Neuroscience 2022Quote: ... muscae by first embedding eight sporulating Canton-S cadavers in a 2.3 cm-diameter disc of ∼3.5 mm thick 5AS that was transferred with 6” forceps into the bottom of an empty wide-mouth Drosophila vial (Genesee #32-118). A ruler was used to mark 1.5 cm above the top of the disc ...
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bioRxiv - Molecular Biology 2023Quote: HEK293T reporter cell lines and the Hepa1-6 stable cell line were cultured in Dulbecco’s Modified Eagle Media (DMEM, Genesee Scientific 25-500) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 5% heat-inactivated fetal bovine serum (GenClone 25-514H, Genesee Scientific) plus 1% penicillin and streptomycin.
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bioRxiv - Developmental Biology 2020Quote: ... with collection from a 4-hour egg-laying interval on grape agar plates (Genesee Scientific) with a small amount of baker’s yeast paste ...
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bioRxiv - Genomics 2022Quote: ... Each 50 μL reaction contained 5 μL of 10X PCR buffer (Genesee, SanDiego, CA), 2.5 μL dNTPs (2 mM per nucleotide) ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was blocked with 5% BSA in Tris-buffered saline (TBS, Genesee Scientific) with 0.1% Tween 20 (TBST ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was blocked overnight at 4°C in OneBlock Western-FL Blocking Buffer (Genesee Scientific), then incubated in the primary antibody at 4°C overnight ...
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bioRxiv - Physiology 2023Quote: ... in HBSS followed by incubation for 1 hour at 37°C with shaking at 1000 RPM in 0.25% Trypsin-EDTA (25-510; Genesee Scientific) supplemented with 10 μM Y27632 ...
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bioRxiv - Biophysics 2021Quote: ... and the cleared supernatant was applied to a 5 ml Ni2+ column (Genesee or Cytivia) and eluted with a 15 column volume gradient from 0-100% Ni2+ B buffer (Buffer A plus 250 mM imidazole) ...
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bioRxiv - Microbiology 2022Quote: ... Mike Diamond and were maintained at 37°C with 5% CO2 in DMEM (Genesee Scientific) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cancer Biology 2022Quote: ... The membranes were then blocked with 5% non-fat dry milk (Genesee Scientific, #20-241) in TBST (0.1% Tween 20 in TBS ...
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bioRxiv - Biochemistry 2024Quote: ... the modified RNA was purified using the RNA Clean & Concentrator-5 kit (Genesee Scientific #R1014). The RNA sample was eluted from the column with 7 μL of RNase-Free water ...
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bioRxiv - Neuroscience 2020Quote: ... and tissue pellet resuspended in astrocyte media (DMEM with 10% fetal bovine serum and 1% penicillin/streptomycin; Cat.# 25-500, Cat.# 25-550, Cat.# 25-512, Genesee Scientific). Tissue pellet was triturated with decreasing size pipettes until a single cell suspension was achieved ...
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bioRxiv - Bioengineering 2023Quote: ... we used 4–8-week-old seedlings grown individually in drosophila vials (Genesee Scientific, San Diego, CA).
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bioRxiv - Genomics 2020Quote: ... The cloned inserts were then transformed into purchased GC-5 cells (Genesee Scientific, El Cajon, CA) for ORF incorporation ...
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bioRxiv - Neuroscience 2021Quote: ... VA) was purchased and maintained in DMEM-10 (DMEM with 10% fetal bovine serum and 1% penicillin/streptomycin: Genesee Scientific, San Diego, CA). C8-D1A cells were modified to express an inducible CreERT2 protein (known further as C8-D1A-CreERT2 astrocytes ...
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bioRxiv - Cell Biology 2023Quote: ... 270 mg total protein was divided evenly into 11 5-mL centrifuge tubes (Genesee Scientific, 24-285) and rotated with FLAG antibody overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... and supernatant was added to 2 70% confluent T-75 flasks (Genesee Scientific, 25-209). Propagation continued and collection repeated for infection of 10 15 cm dishes ...
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bioRxiv - Immunology 2020Quote: All immunoprecipitation experiments were conducted in 2 ml deep-well polypropylene plates (Genesee Scientific, Inc). Plates were blocked with 3% BSA in TBST overnight with overhead rotation on a Rotator Genie prior to use for immunoprecipitations ...
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bioRxiv - Genetics 2021Quote: Flies were grown and maintained on food consisting of the following ingredients: 1 part of Nutri-Fly® GF (Genesee Scientific, cat. 66-115) and 3 parts of Jazz-mix (Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: HeLa cells were cultured in 5% CO2 on cell culture-treated 10 cm plates (Genesee Scientific, 25-202) in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Biophysics 2022Quote: HeLa cells were cultured in 5% CO2 on cell culture-treated 10 cm plates (Genesee Scientific, 25-202) in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... with the 5 concentrations and the 0.5% DMSO control in separate rows of each 48-well plate (Genesee Scientific ...
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bioRxiv - Microbiology 2023Quote: ... membranes were developed for 5 min with the Prometheus™ ProSignal Femto ECL reagent (Genesee Scientific 20-302) and imaged with 10 sec exposure using a BioRad ChemiDoc MP Imaging System.
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bioRxiv - Cell Biology 2023Quote: ... we removed 5 mL of supernatant and filtered it through 0.45 µm PES syringe filters (Genesee Scientific, #25-246) and stored the filtrate at 4 °C ...
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bioRxiv - Biophysics 2023Quote: HEK293T cells were cultured in a humified incubator at 37°C and 5% CO2 in high-glucose Dulbecco’s Modified Eagle Medium (DMEM) plus sodium pyruvate (Genesee) and supplemented with 10% FBS and penicillin (100 units/ml)/streptomycin (100µg/ml) ...
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bioRxiv - Biochemistry 2024Quote: ... and it was included in the RNA Clean and Concentrator-5 with the DNase I kit (Genesee Scientific #R1014). The RNA was then purified using the same kit ...
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bioRxiv - Microbiology 2023Quote: ... for 2 h and then filtered using a vacuum-driven 0.22 µm filter (Olympus plastics, Genesee Scientific) prior to the inoculation of cells to remove any remaining trace of metal ...
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bioRxiv - Neuroscience 2021Quote: ... The top of the acrylic box had an entry port of 4” x 2.7” for CO2 diffused by a Flystuff Flypad (Genesee Scientific, 59-114), and two doors on the side ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR was performed in triplicate using 10μl reactions (2.5μl of cDNA, 5 μM of each forward and reverse primers, and 2x SYGreen mix (Genesee #17-505B)) ...
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bioRxiv - Microbiology 2021Quote: ... CFCS was collected by centrifugation at 4,000 x g for 10 min at 4 °C followed by filtration of the supernatant through a 0.45 μm polyethersulfone (PES) filter (Genesee Scientific, San Diego, CA). To eliminate the effects of differences of pH on yeast inhibition ...
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bioRxiv - Genetics 2023Quote: ... RNA was isolated from the Trizol aqueous phase using the Zymo RNA Clean and Concentrator-5 kit (Genesee Scientific #11-352) including treatment with DNAse I ...
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bioRxiv - Immunology 2023Quote: ... The cell pellet was resuspended in 5 ml of the 70% fraction of a 70:37 Percoll gradient and overlaid on 5 ml of the 37% fraction in a 15 ml Falcon tube (Genesee Scientific). Percoll gradient separation was performed by centrifugation without brakes for 20 minutes at 1500 rpm ...
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bioRxiv - Neuroscience 2022Quote: ... Approximately 330 mg of 0–5-day-old Canton-S flies were transferred to a small embryo collection cage (Genesee #59-100) which was topped with the dish containing the cadavers ...
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bioRxiv - Microbiology 2023Quote: ... were maintained at 37°C in 5% CO2 in as monolayer cultures in DMEM media with L-Glutamine and sodium pyruvate (Genesee # 25-500). NIH3T3 cell media was supplemented with 10% newborn calf serum (NBCS ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... newly eclosed flies were anaesthetized with CO2 and groups of 5 virgin females were transferred into narrow Drosophila vials (Genesee Scientific #:32-109) containing SY media for 5 days ...
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bioRxiv - Microbiology 2022Quote: Serum samples were serially diluted in RPMI-1640 with 10 mM HEPES and 2% FetalPure bovine serum (Genesee Scientific 25-525H), hereafter referred to as viral diluent ...
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bioRxiv - Genetics 2023Quote: ... Flies were expanded in vials (23 mm X 95 mm) containing ∼ 5 mL of a standard cornmeal-based medium for larval growth (Genesee Scientific, Cat#: 66-113) adapted from the Bloomington Drosophila Stock Center (https://bdsc.indiana.edu) ...
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bioRxiv - Genetics 2023Quote: ... Separated flies were then transferred by sex to respective sucrose-yeast experimental food vials with fixed 5% [w/v] sucrose (Genesee Scientific, Cat#: 62-112) and varying yeast (Brewer’s yeast ...
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bioRxiv - Cell Biology 2020Quote: sgRNA transduced cells seeded on 6-well plates at ~60% confluency were transfected with 2 μg flag-tagged β2-AR for 48 hr using JetPrime Transfection reagent (Genesee, Cat #55134) following manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: ... brucei Lister 427 were grown in 5 ml of modified HMI-9 medium46 in T25 suspension cell culture flasks (Genesee Scientific, Cat. No. 25-213) under a humidified atmosphere of 5% CO2 at 37 °C ...