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Citations for Biotek :
1 - 50 of 319 citations for hsa mir 32 Real Time RT PCR Detection and U6 Calibration Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... and subsequently bead/size selection of RT/PCR products (TotalPure NGS, Omega Biotek). Three rounds of nested PCR using Phusion HF 2x Master Mix (New England Biolabs ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... with luminescence measured in real-time every 2 min for 12 min (Biotek Neo 2 plate reader). The peak luminescence responses (typically measured at 12 min ...
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bioRxiv - Immunology 2021Quote: ... 5% CO2 and real-time monitoring of NK cell killing was performed on a Cytation(tm) 5 (Biotek). Cell images were processed using GEN5 software (Biotek) ...
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bioRxiv - Immunology 2023Quote: ... and the PI up-take was measured every 5 min on a real-time fluorimeter (Synergy Biotek Instruments) (Case & Roy ...
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bioRxiv - Biochemistry 2020Quote: ... 0.05% Tween20) for 1 h at RT and then washed 3 times with H2O and 0.05% Tween20 (BioTek Instruments, EL405). Afterwards ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR product was subsequently cleaned up using PCR cleanup kit (Omega Biotek) and stored in dH2O ...
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bioRxiv - Synthetic Biology 2022Quote: Real-time OD600 measurements were obtained every 10 min for approximately 72 hours with microplate reader Synergy™ H1 (BioTek) for aerobic and microaerobic conditions ...
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bioRxiv - Cancer Biology 2019Quote: ... DNA concentration was measured by SYBR green fluorescence with a λ dsDNA calibration curve (readings on BioTek Synergy H1). ClonTracer DNA barcodes consist of a repeating ‘Strong (G or C ...
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bioRxiv - Immunology 2022Quote: ... We measured the cGAMP concentration in the filtrates against a calibration curve of cGAMP at 260 nm using Take3 Micro-Volume absorbance analyzer of Cytation 5 (BioTek). We calculated the final concentration of cGAMP in NanoSTING and encapsulation efficiency by subtracting the concentration of free drug in the filtrate.
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bioRxiv - Immunology 2022Quote: ... We measured the cGAMP concentration in the filtrates against a calibration curve of cGAMP at 260 nm using Take3 Micro-Volume absorbance analyzer of Cytation 5 (BioTek). We calculated the final concentration of cGAMP in NanoSTING and encapsulation efficiency by subtracting the concentration of free drug in the filtrate.
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bioRxiv - Immunology 2023Quote: ... The CpG concentration on the NPs was determined through absorption calibration curves at 280 nm acquired using a Synergy H1 Microplate Reader (BioTek Instruments). An individual calibration curve for each NPs valency and TLR9 agonist class was recorded ...
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bioRxiv - Microbiology 2023Quote: ... incubated at 32°C with double-orbital shaking in an Epoch 2 microplate reader (BioTek, Germany), and analysed photometrically (OD600 ...
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bioRxiv - Immunology 2022Quote: ... and lambda chains.48 The products from the second PCR were analyzed by agarose gel electrophoresis and purified PCR products (ENZA cycle pure kit; Omega Biotek) were submitted to Genewiz for sequencing ...
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bioRxiv - Synthetic Biology 2023Quote: ... The product was subsequently cleaned up using PCR cleanup kit (Omega Biotek) and stored in dH2O ...
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bioRxiv - Microbiology 2022Quote: ... followed by luminescence detection (Synergy H1, BioTek). Data are expressed as Relative Light Units (RLU ...
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bioRxiv - Molecular Biology 2020Quote: ... and PCR products were purified using the EZNA Gel Extraction kit (Omega BioTek).
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bioRxiv - Microbiology 2023Quote: ... Growth was then followed at 32 °C under double-orbital shaking in an EPOCH 2 microplate reader (BioTek, USA) by measuring the OD580 at 20-min intervals.
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bioRxiv - Microbiology 2023Quote: ... recorded using a Synergy2 Multi-Detection Microplate Reader (BioTek) at the indicated time points post-infection.
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bioRxiv - Bioengineering 2023Quote: ... and grown for 32 h at 30 °C with their lids on and continuous shaking in a Cytation 5 microplate reader (BioTek). High-resolution growth curves were generated from Attenuance (D600 nm ...
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bioRxiv - Biophysics 2020Quote: ... after 48h using a Synergy2 Multi-Detection Microplate Reader (BioTek)
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bioRxiv - Immunology 2020Quote: ... Detection was done in a Synergy M1 Plate reader (Biotek). When absolute quantitation is presented ...
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bioRxiv - Immunology 2021Quote: ... Detection was done in a Synergy M1 Plate reader (Biotek). When absolute quantitation is presented ...
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bioRxiv - Cancer Biology 2024Quote: ... according to the manufacturer’s protocol and as described previously.(26, 32) The absorbance was recorded at 490Lnm using Biotek’s Synergy Neo2 multimode plate reader (Biotek, Winooski, VT). The half-maximal inhibitory concentration (IC50 ...
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bioRxiv - Microbiology 2022Quote: ... followed by EGFP signal detection measured with the Synergy 4 (BioTek). EC50s were calculated with a 4-parameter logistic model using XLfit software (IBDS).
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bioRxiv - Plant Biology 2023Quote: ... Luminescence detection was conducted to measure ROS production (BioTek, Beijing, China).
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bioRxiv - Immunology 2020Quote: ... Samples were analyzed by agarose gel electrophoresis and purified PCR products (ENZA cycle pure kit; Omega Biotek) were cloned into the pCR2.1 vector using the Original TA cloning kit (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... The OD600 was measured at 10 min intervals for 20 hr at 32°C in orbital shaking mode on a SynergyH1 plate reader (Biotek, Winooski, VT, USA). Growth data for each strain was obtained from three replicate wells and three independent cultures ...
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bioRxiv - Biochemistry 2020Quote: ... The −500 to +216 region corresponding to the HSPA1B promoter was amplified from the plasmid by PCR with Phusion polymerase (Thermo-Fisher #F530S) and purified via E.Z.N.A Gel Extraction Kit (Omega BioTek #D2500). The DNA was ethanol precipitated ...
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bioRxiv - Microbiology 2020Quote: ... 560nm excitation/610nm emission detection with a Synergy H1 microplate reader (Biotek) gave equivalent results ...
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bioRxiv - Biochemistry 2020Quote: A Synergy 2 Multi-Detection 96-well Microplate Reader (BioTek, Winooski, VT) was used at 37 °C for experiments assessing complex II activity over the pH range 6.6–7.8 ...
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bioRxiv - Biochemistry 2020Quote: A Synergy 2 Multi-Detection 96-well Microplate Reader (BioTek, Winooski, VT) was used at 37 °C for experiments assessing ROS generation as result of RET over a pH range ...
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bioRxiv - Cell Biology 2021Quote: ... Luminescence was measured with the Synergy HT Multi-Detection Microplate Reader (BioTek) and was normalized against control cells treated with DMSO.
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bioRxiv - Neuroscience 2020Quote: ... luminescence was recorded using a Synergy 2 Multi-Detection Microplate Reader (BioTek) with a read height of 1mm ...
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bioRxiv - Molecular Biology 2021Quote: ... and wells were washed three times with 300 μl of diluted washing solution (soak time > 5 seconds) using an automated plate washer (BioTek 50TS). After washing ...
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bioRxiv - Synthetic Biology 2021Quote: ... Both fragments were cleaned with the Viogene Gel/PCR DNA Isolation Kit (Viogene-Biotek Corporation, New Taipei, Taiwan R.O.C.) and then fused in an overlapping PCR to make an IS1 fragment lacking inverted repeats but having SspI sites on the ends and an XhoI site in the center ...
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bioRxiv - Cell Biology 2022Quote: ... Assay detection was performed using the Synergy Mx Multi-Mode Microplate Reader (BioTek).
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bioRxiv - Cell Biology 2021Quote: ... incubated for 45 min at RT and read on a luminometer (Synergy2, BioTek) to obtain relative units (RU) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The OD600 was measured at the indicated time (BioTek). Competitive growth for EcN and S ...
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bioRxiv - Cell Biology 2020Quote: ... time-lapse red fluorescence images were acquired by BioTek Lionheart FX Automated Microscope with 1s interval of 10s and followed by fMLP injection and image recording for another 2 min with 1s interval ...
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bioRxiv - Biochemistry 2022Quote: ... Oligonucleotides were converted to double-stranded DNA using 15 cycles of PCR amplification and purified on a spin column (EZNA Cycle Pure kit from Omega BioTek). A pSC101 backbone containing extant E ...
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bioRxiv - Bioengineering 2022Quote: ... Luminescence detection was performed by an inspired plate reader (Biotek, Montpelier, VT, United States).
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bioRxiv - Physiology 2020Quote: ... Endpoint fluorescence was measured using a Synergy™ multi-detection microplate reader (BioTek Instruments) with the following excitation and emission wavelength ...
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bioRxiv - Molecular Biology 2023Quote: ... luciferase activity analysis was examined using Fluorescence/Multi-Detection Microplate Reader (BioTek, Winooski, USA) and Dual-GLO®Luciferase Assay System Kit (Promega) ...
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bioRxiv - Immunology 2021Quote: ... uptake over time using a fluorescent plate reader (Cytation5; Biotek). Cell lysis was quantified by measuring the amount of intracellular LDH release into the cell culture supernatant (TaKaRa LDH cytotoxicity detection kit ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell viability was then measured by luminescence detection using a Synergy H1 microplate reader (BioTek).
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bioRxiv - Plant Biology 2023Quote: ... and 750nm using a spectrophotometer plate reader (Synergy HT Multi-Detection Microplate Reader; BioTek Instruments). Chlorophyll A was calculated as 13.71*(664nm-750nm)-2.858*(647nm-750nm) ...
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bioRxiv - Cell Biology 2023Quote: ... and light emission was immediately measured by the luminescence detection mode in Cytation 5 (Biotek). Luciferase activities were normalized to cell density measured by OD600 and adjusted to total abundance of FlucSM protein measured by immunoblotting.
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bioRxiv - Synthetic Biology 2021Quote: ... Both products were DpnI digested and cleaned with the Viogene Gel/PCR DNA Isolation Kit (Viogene-Biotek Corporation, New Taipei, Taiwan R.O.C.). These fragments were assembled in a Circular Polymerase Extension Cloning (CPEC ...
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bioRxiv - Genetics 2020Quote: ... washed four times using a 406PUB1 microplate washer (BioTek, VT, USA), and stained with 50 μL Sytox Orange at 1 μM to fluorescently stain dead worms ...
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bioRxiv - Neuroscience 2020Quote: ... β-galactosidase expression was measured using colorimetric detection (Abs420nm, using a Synergy H1 plate reader (Biotek) from samples in a 96-well plate ...