Labshake search
Citations for Biotek :
501 - 550 of 677 citations for Rat Angiopoietin 2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... Fluorescence was measured for 2 h at excitation and emission on a Synergy HTX Multi-Mode Microplate Reader (BioTek, Winooski, VT).
-
bioRxiv - Microbiology 2023Quote: ... transferred to a 96-well plate and incubated at 30°C with double-orbital shaking at 355 cpm in an Epoch 2 microplate reader (BioTek, Germany). Growth was monitored by measuring the OD600 at 10-min intervals until the stationary phase was reached ...
-
bioRxiv - Microbiology 2023Quote: ... The cultures were grown under strong agitation at 37°C and analyzed in Synergy 2 Multi-mode microplate reader (BioTek Instruments). Relative Luminescence Units (RLU ...
-
bioRxiv - Microbiology 2024Quote: ... Free phosphate released upon hydrolysis of the TDP or UDP produced by RmlT was quantified by absorbance at 620 nm using a Synergy 2 multi-mode plate reader (BioTek, USA). RmlT reaction mixtures were also separated in 20 % polyacrylamide gels and WTA were stained with 1 mg mL-1 Alcian Blue solution after overnight incubation.
-
bioRxiv - Microbiology 2024Quote: ... Cells were washed three times with PBS and binding to the bacterial cell wall was visualized using a fluorescence microscope Olympus BX53 or quantified using a plate reader Synergy 2 (BioTek, USA).
-
bioRxiv - Synthetic Biology 2021Quote: ... The amplicon was then purified using a Cycle Pure Kit (Omega Biotek). The vector backbone fragment was amplified from pET28a-M-CaM-cTdTS plasmid DNA constructed above using PfuUltra II Hotstart PCR Master Mix using DNA primers P29 – P36 listed in Table 1 ...
-
bioRxiv - Cell Biology 2022Quote: ... and DNase-treated using commercially available kits (Omega Biotek and Ambion, respectively). Libraries were constructed and subsequently subjected to 151bp paired-end cycles on the NovaSeq-6000 platform (Illumina) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... PCR product was subsequently cleaned up using PCR cleanup kit (Omega Biotek) and stored in dH2O ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The product was subsequently cleaned up using PCR cleanup kit (Omega Biotek) and stored in dH2O ...
-
bioRxiv - Cell Biology 2023Quote: ... RNA was extracted using an Omega total RNA extraction kit (Omega Biotek) (column-based system ...
-
bioRxiv - Bioengineering 2020Quote: ... the perfusate from both the fetal and maternal channels was collected and its fluorescence intensity quantified utilizing a microplate reader (BioTek Synergy 2).
-
bioRxiv - Evolutionary Biology 2022Quote: ... Chlorophyll a and b were extracted using an acetone and alcohol protocol(Zhao et al., 2018) and quantified using Synergy™2 multi-detection microplate reader (Biotek, VT). Light response curve was measured based on the red and blue light sources using Li 6800 (Li-COR ...
-
bioRxiv - Molecular Biology 2021Quote: Fluorescence intensities were measured by a Cary Eclipse spectrofluorometer (Varian, Palo Alto, CA) for single time point measurements and a Synergy 2 plate reader (Biotek, Winooski, VT) for multiple time point measurements.
-
bioRxiv - Biochemistry 2019Quote: ... Chemiluminescence was measured every 1-2 minutes over 1.5 hours at 37 °C using the Synergy H1 multi-detection microplate reader (Biotek, Bad Friedrichshall, Germany) in triplicates and chemiluminescence activity was expressed as integrated total counts as calculated by the addition of rectangles with unit width under individual data points ...
-
bioRxiv - Physiology 2019Quote: ... and an aliquot of the synthesized RNA probes were then visually assessed using standard agarose gel electrophoresis and quantified on a SYNERGY 2 Microplate reader (Biotek, Winooski, VT).
-
bioRxiv - Physiology 2019Quote: ... The final purified PCR products for use as templates for RNA probe synthesis were quantified on a SYNERGY 2 Microplate reader (Biotek, Winooski, VT).
-
bioRxiv - Physiology 2019Quote: ... aegypti CAPAr were loaded into each well of multi-well plate using an automated injector module linked to a Synergy 2 Multi Mode Microplate Reader (BioTek, Winooski, VT) which measured kinetic luminescent response from each well for 20 sec immediately following cell loading onto the different peptides at various doses ...
-
bioRxiv - Microbiology 2020Quote: ... OD600 recordings were taken every 30 minutes for 44 hours and then every 60 minutes for 96 hours (6 days total) with an Epoch 2 Microplate Spectrophotometer (Biotek, Winooski, VT) at 45°C within a rigid gloveless hypoxic chamber (Coy Lab ...
-
bioRxiv - Microbiology 2020Quote: Pure cultures of defined mutants were diluted to A600 0.003 and grown +/− antibiotic in 96-well microtiter format at 37°C with shaking in a plate reader (Tecan M200 Pro, Biotek Epoch 2, or Biotek Synergy H2M). Growth was monitored as change in A600 ...
-
bioRxiv - Microbiology 2021Quote: ... The 96-well plates were incubated anaerobically in a temperature-controlled plate reader at 37°C (Epoch 2 Microplate Spectrophotometer, BioTek, Swindon, UK). For each test and technical replicate ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2 h later the fluorescence was read at 340/505 and 380/505 on a Cytation™ 3 (BioTek (Vermont, MA, USA)) multifunctional microplate reader ...
-
bioRxiv - Biochemistry 2020Quote: ... Ten parallel bacterial cultures of 100 μL for each condition were shaken in a Synergy 2 microplate reader (BioTek, Winooski, VT, USA) at 37 oC ...
-
bioRxiv - Bioengineering 2021Quote: ... Then the plate was vortexed at 400 rpm for 2 min and luminescence read on a Synergy Neo2 plate reader (BioTek, Winooski, VT).
-
bioRxiv - Microbiology 2021Quote: ... The OD600 was used to monitor growth during incubation at 30 °C for 48 h without aeration using a Synergy 2 microplate reader (Biotek, Winooski, VT).
-
bioRxiv - Biochemistry 2021Quote: ... and inhibitor (APPI monomer, 0-400 nM; APPI dimer, 0-96 nM) in a Synergy 2 Multi-Detection Microplate Reader (BioTek, VT, USA). Mesotrypsin buffer (296 μL) ...
-
bioRxiv - Biochemistry 2021Quote: ... Fluorescent polarization was read parallel and perpendicular to the excitation plane (ex. 485 nm/ em. 528 nm) using a Synergy 2 Microplate reader (BioTek, Winooski, VT) and the relationship between signal and time was linear (Figure S2) ...
-
bioRxiv - Cancer Biology 2020Quote: ... and the plate was returned to incubator for 2 hours after which absorbance was read at 450 nm in a microplate reader (BioTek Instruments Inc).
-
bioRxiv - Microbiology 2020Quote: ... Serosal samples were collected every 30 minutes for 2 hours with FITC dextran flux (ng/ml/cm2/hr) determined by plate reader (Biotek Synergy H1).
-
bioRxiv - Immunology 2021Quote: ... and light emission was measured every 2 minutes for a period of 120 min using the Synergy H1 microplate reader (Biotek Instruments, USA). The light emission levels are expressed as RLU (Relative Light Units = luminescence measured by the luminometer).
-
bioRxiv - Plant Biology 2022Quote: ... The DNA was normalized to 5 ng/μL based on quantification with a Synergy 2 multimode microplate reader (Biotek Instruments, Winooski, VT) with the Quant-iT PicoGreen double-stranded DNA quantification assay (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... were incubated in a 96-well flat-bottom plate (Nunclon) at 37°C with continuous shaking (double orbital) in a microplate spectrophotometer (BioTek Epoch 2). Optical densities were recorded every 20 min ...
-
bioRxiv - Plant Biology 2022Quote: ... The reaction was followed by measuring the absorbance at 363 nm at 25°C for 15 min using a plate reader (BioTek Synergy 2). For NR activity measurements on material extracted from Arabidopsis ...
-
bioRxiv - Cancer Biology 2022Quote: ... and day 2 by addition of the CTG reagent followed by measurement of luminescence using the Biotek Cytation 5 (BioTek, Winooski, VT). Luminescence values were adjusted based on 2µM Adenosine triphosphate (ATP ...
-
bioRxiv - Plant Biology 2023Quote: ... The background luminescence of seedling was recorded for 2 min before application of flg22 and chitin (5 s for NaCl) on a microplate reader (BioTek Synergy H1). Then 50 μl of 200 nM flg22 ...
-
bioRxiv - Immunology 2023Quote: ... This was achieved by collecting samples from the serosal compartments every 30 minutes for 2 hours and measuring the respective FITC concentrations on a plate reader (Synergy H1; BioTek, Winooski, VT). Samples with visible FITC leakage in the serosal compartment were omitted ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The absorbance at 405 nm was measured every 1 min for at least 30 min and up to 2 h using a Synergy 4 microplate reader (Biotek, Winooski, VT). SEAP activities were calculated as a function of the increase in absorbance at 405 nm over time42.
-
bioRxiv - Genetics 2023Quote: ... The plates were sealed with clear plastic adhesive film and incubated at 30°C with no shaking in a microplate spectrophotometer (BioTek Epoch 2) with OD600 readings taken of each well every 30 minutes for 48 hours ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The O.D./min was continuously recorded at 405 nm over 30 min to measure the hydrolytic product 5-mercapto-2-nitrobenzoic acid using a Synergy H1 microplate reader (Biotek; VT, USA). O.D./min was converted to catalytic rate using the Beer-Lambert law:
-
bioRxiv - Cell Biology 2020Quote: ... The purified extracts of CanlonicSF were excited at 485 ± 20 nm and the intensity of fluorescence emission of cpGFP was recorded at 530 ± 15 nm using the multiwell plate analyzer Synergy 2 (Biotek, Winooski, Vermont, USA). Excitation and emission spectra were obtained by collecting the emissions at 515 ± 15 nm using a multiwell plate reader EnVision (PerkinElmer ...
-
bioRxiv - Immunology 2022Quote: ... Reactions were stopped by the addition of 2M sulfuric acid and read using a BioTek Synergy 2 or Synergy H1 plate reader set at 450nm (BioTek, Winooski, VT, USA). Receiver operator curve (ROC ...
-
bioRxiv - Physiology 2020Quote: ... The absorbance was determined spectrophotometrically at a wavelength of 450 nm using a spectrophotometer (Epoch 2 microplate spectrophotometer, BioTek Instruments, Inc, Vermont, USA).
-
bioRxiv - Biochemistry 2021Quote: We measured the catalytic activity of the enzyme citrate synthase in mitochondrial samples monitoring the conversion of oxaloacetate and acetyl-CoA to citrate and CoA– SH and by measuring the formation of the colorimetric product thionitrobenzoic acid (TNB) at 412 nm and 37°C (Shepherd and Garland, 1969) on a microplate reader (Power Wave XS-2, Biotek Instruments, Winooski, USA). We calculated the enzyme activity using the changes in absorbance after substrate (250 µM oxaloacetate ...
-
bioRxiv - Neuroscience 2020Quote: ... Fluorescence post-thallium addition was monitored every 21 seconds for 600 seconds total using a BioTek Synergy 2 (BioTek, Winooski, VT, United States) multi-well plate reader equipped with an excitation filter of 485/20 nm and emission filter 528/20 nm ...
-
bioRxiv - Microbiology 2022Quote: ... The cells were grown with agitation at 30°C for 30 hours in a microplate reader (Infinite M Plex; Tecan, Austria, or Synergy 2; BioTek, Winooski, VT, USA), and the optical density at 600 nm (OD600 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Aerobic growth was monitored in technical duplicates or triplicates at 37°C in a BioTek Epoch 2 Microplate Spectrophotometer (BioTek, Bad Friedrichshall, Germany) by absorbance measurements (600nm ...
-
bioRxiv - Microbiology 2021Quote: ... the cells were collected by centrifugation at 10,000 g for 5 min and the absorbance of the supernatant was measured at 562 nm with a Synergy 2 spectrophotometer (BioTek, Winooski, VT, USA). Quantities of ferrihydrite reduced were determined using a standard curve containing a 2-fold range of FeSO4 (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2021Quote: ... was grown at 27°C on a 96-well plate (200 μL) and absorbance at 600 mm was recorded on a microplate reader (Synergy 2 Multi-Mode Microplate Reader, BioTek, Winooski, Vermont, USA). Non-inoculated media were used as blank ...
-
bioRxiv - Cell Biology 2021Quote: ... was measured every 5 min for 2 h at 25°C using Synergy H1 Hybrid Multi-Mode Microplate Reader (BioTek, cat. no. H1MFDG). The rate of kinetic reaction (slope ...
-
bioRxiv - Immunology 2020Quote: ... Growth was measured as relative light units at 528 nm after excitation at 485 nm in a fluorescence microplate reader (Synergy 2, BioTek Instruments, Vermont, USA) at the indicated time points.
-
bioRxiv - Genomics 2021Quote: ... absorbance was measured for each well at 435 nm and 545 nm on a Synergy 2 plate reader (Biotek Instruments, Winooski, VT, USA). Because of variation in background absorbance from well to well on each plate ...