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Citations for Biotek :
101 - 150 of 164 citations for RT PCR kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Cell-associated viral RNA was extracted from infected cells using the E.Z.N.A Total RNA kit (Omega-BioTek). Viral RNA was reversed transcribed and quantified by qPCR.
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bioRxiv - Genomics 2021Quote: Viral load quantification: viral RNA was extracted from Mucilair apical washes using EZNA viral extraction kit (Omega Biotek) and quantified by using RT-qPCR with the QuantiTect kit (#204443 ...
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bioRxiv - Microbiology 2021Quote: Samples were prepared and DNA was extracted using the E.Z.N.A Soil DNA kit (Omega Biotek, Norcross, GA, USA). The tips of the swabs were placed in disruptor tubes by using a sterilized scissor ...
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bioRxiv - Developmental Biology 2022Quote: ... total RNA from the embryos was extracted with an OMEGA Total RNA extraction kit I (Omega Biotek, USA). To remove genomic DNA ...
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bioRxiv - Genomics 2019Quote: ... Haploid megagametophyte tissue was used for genomic DNA material extraction by using E.Z.N.A.® SP Plant DNA kit (Omega Biotek). DNA was fragmented to an average length of 200 nucleotides with Bioruptor® ultrasonicator ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were harvested three days post transfection using E.Z.N.A Total RNA Kit 1 (Omega Biotek cat #R6834-01) according to the manufacturer’s instructions ...
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The differential distribution of bacteria between cancerous and noncancerous ovarian tissues in situbioRxiv - Microbiology 2019Quote: DNA extractions were performed by using the Mag-Bind® Stool DNA 96 Kit (Omega Biotek, Norcross, USA). DNA was quantified using the QuantiFluor dsDNA System (Promega ...
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bioRxiv - Evolutionary Biology 2022Quote: Genomic DNA was isolated from the tissue samples of Þistilfjörður population using the E-Z 96 Tissue DNA Kit (Omega Biotek) according to the manufacturer’s recommendation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Genomic DNA for landraces was extracted from leaf tissue using the E.Z.N.A.® Plant DNA Kit (Omega Biotek), following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Supernatant protein concentrations were quantified using a BCA Protein Assay kit (Pierce) and a spectrophotometric plate reader (BioTek). Most protein lysates were separated by running 30-100 μg of lysate on 10% Bis-Tris NuPage gels (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... DNA was extracted from single adult weevils for each species using the EZNA Insect DNA Kit (Omega Biotek) and quantified using a Nanodrop 2000 ...
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bioRxiv - Microbiology 2020Quote: Microbial DNA was extracted from the samples using an E.Z.N.A.® Stool DNA Kit (Omega BioTek, Norcross, GA, USA) according to manufacturer’s protocols ...
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bioRxiv - Zoology 2019Quote: Whole genomic DNA was extracted from tissues using either Chelex or an E.Z.N.A Tissue DNA Kit (Omega BioTek, Norcross, GA). The four genes 16S ...
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bioRxiv - Microbiology 2022Quote: ... DNA was immediately extracted from a portion of the zooplankton tissue mass via the EZNA tissue kit (Omega Biotek) and was used to create a highly concentrated standard whose DNA concentration was measured with a Qubit 2.0 (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: RNA was extracted from swab or tissue supernatant using the Mag-Bind Viral DNA/RNA 96 Kit (Omega Biotek) on the KingFisher FLEX System (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was extracted from frozen root material using the E.Z.N.A.® Plant RNA Kit with the associated RNase-free DNase I Set (Omega-Biotek, USA), following the standard protocol provided ...
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bioRxiv - Developmental Biology 2024Quote: ... and the band located at a 7.9 kb mark was cut and purified using the EZNA Cycle pure kit (Omega Biotek) Purified digested vector and inserts were ligated in a 1:30 vector/insert ratio using the T4 DNA ligase (New England Biolabs ...
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bioRxiv - Synthetic Biology 2024Quote: ... the proper band was isolated using gel extraction on a 0.5% agarose gel followed by purification with the EZNA gel extraction kit (Omega BioTek). The concentration of the DNA was measured using AccuClear (Biotium ...
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bioRxiv - Cancer Biology 2023Quote: ... the total RNA was extracted using the E.Z.N.A column-based total RNA kit (Omega BioTek, catalog number R6834-01) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... The solubilized protein was quantified by BCA Assay Kit (Fisher) in triplicates at 590nm using a microplate reader (Synergy HT, BioTek). 15 to 20 µg of ß-mercaptoethanol denatured protein were loaded in Tris-glycine polyacrylamide gels (14% ...
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bioRxiv - Neuroscience 2020Quote: ... The rats’ insulin levels were measured using an enzyme-linked immunosorbent assay (ELISA) kit (Hangzhou Eastbiopharm Co., Ltd., Hangzhou, China) and a spectrophotometer (Biotek Epoch™ Microplate Spectrophotometer ...
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bioRxiv - Microbiology 2020Quote: ... Each well was washed once with 1 mL 1X PBS and RNA was extracted using the EZNA Total RNA kit (Omega Biotek). 375 μL of TRK lysis buffer with β-Mercaptoethanol was added to each well and incubated for 3 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... or human IFN-β (1000 U/ml) and gaussia luciferase activity was determined using Gaussia Luciferase Glow Assay Kit (Thermo) on Cytation 3 (BioTek) multimode plate imager according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was isolated from clarified cell culture supernatants using an Omega Total RNA Isolation Kit 1 (Omega BioTek, VWR) and reverse transcribed at 42°C using an oligo dT primer and SuperScript II reverse transcriptase (Life Technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR products were purified by agarose gel and extracted using a GFX gel band extraction kit according to the manufacturer’s instruction (Omega Biotek, USA). 500ng of purified PCR fragments were used to prepare Digoxigenin or Fluorescein labelled antisense probes ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein quantification was performed using the Pierce BCA protein assay kit (ThermoScientific) and analyzed on a Cytation 3 plate reader (BioTek). Proteins were separated by NuPAGE 4-12% Bis-Tris Gel and transferred to a nitrocellulose membrane ...
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bioRxiv - Physiology 2021Quote: All serum insulin concentrations were quantified using a commercially available insulin immunoassay kit (32380; Toronto Bioscience) as per manufacturer’s instructions using Synergy H4 Hybrid microplate reader (BioTek Instruments). Insulin concentrations were interpolated from a standard curve determined by curve of 4-parameter as per manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: 72 hours after transfection cells were harvested and RNA extraction was performed using E.Z.N.A Total RNA Kit 1 (Omega Biotek cat #R6834-01). cDNA generation was performed using SuperScript IV Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: ... Oligos were converted to double-strand DNA using qPCR and purified on a spin column (EZNA Cycle Pure kit from Omega BioTek). The pSC101 backbone was amplified with two separate primer pairs encoding BsaI cut sites that matched the insertion location of the oligos on the TtgR gene ...
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bioRxiv - Plant Biology 2022Quote: ... Leaf tissue was flash frozen in liquid nitrogen and RNA extracted using the EZNA Plant RNA Kit I (Omega Biotek). DNA contamination was removed by treating total RNA with Turbo DNase with inactivation reagent (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA was isolated from frozen liver samples and DNase treated using commercially available kits (Omega Biotek and Ambion, respectively). Libraries were constructed and subsequently subjected to 151 bp paired-end cycles on the NovaSeq-6000 platform (Illumina) ...
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bioRxiv - Microbiology 2022Quote: Total microbial DNA was extracted from the fecal samples using the OMEGA Soil DNA Kit (Omega BioTek, Norcross, GA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Lipid hydroperoxides were extracted from neuronal lysates and neuron-conditioned media and analyzed using a lipid hydroperoxide assay kit according to the manufacturers protocol and Synergy Mx Multi-Mode Microplate Reader (BioTek). VAMP7 and syntaxin 4 knockdown neurons used for the lipid hydroperoxide assay were transduced at an MOI 7.5.
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bioRxiv - Physiology 2023Quote: ... Soluble host protein of the supernatant was measured using the Pierce BCA Protein Assay Kit (Pierce Biotechnology, Waltham, Massachusetts, Catalog #23225) with two duplicates per sample measured by a 96 well microplate reader (BioTek Synergy HTX Multi-Mode Reader Model S1LFA ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA concentration measurements were performed using the Qubit dsDNA high sensitivity assay kit on the Synergy H1 Hybrid Microplate Reader (BioTek). Libraries were generated using the Nextera XT Library Prep kit (Illumina ...
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bioRxiv - Molecular Biology 2019Quote: ... All kits were performed according to manufacturer's instructions and plates were read using a 96-well spectrophotometer (BioTek, Winooski, VT, USA). Due to missed time points and one subject demonstrating exceptionally high PRE serum CK activity values ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from pure cultures using the Omega Mag-Bind Universal Pathogen Kit according to manufacturer’s protocol with a final elution volume of 60µl (Omega Biotek Inc., Norcross, GA). DNA samples were quantified using Qubit fluorometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from pure cultures using the Omega Mag-Bind Universal Pathogen Kit according to manufacturer’s protocol with a final elution volume of 60μl (Omega Biotek Inc., Norcross, GA). DNA samples were quantified using Qubit fluorometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from pure cultures using the Omega Mag-Bind Universal Pathogen Kit according to manufacturer’s protocol with a final elution volume of 60μl (Omega Biotek Inc., Norcross, GA). DNA samples were quantified using Qubit fluorometer (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: Total RNA extraction from H4 cells was carried out using the E.Z.N.A.® Total RNA Kit I (Omega Biotek, Norcross, GA, USA), and either purity (260nm/280nm ratio and 260nm/230nm ratio ...
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bioRxiv - Plant Biology 2020Quote: ... and total RNA was extracted using the protocol for difficult samples in E.Z.N.A.® Plant RNA Kits (Omega Biotek, Norcross, GA, USA). The samples were reverse transcribed and specific sequences were amplified using the SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... the firefly LUC and Renilla LUC activities were measured by Dual Luciferase Reporter Gene Assay Kit (Yeasen, Shanghai) and Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, USA). Activity is expressed as the ratio of firefly LUC activity to Renilla LUC activity ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from pure cultures using the Omega Mag-Bind Universal Pathogen Kit according to manufacturer’s protocol with a final elution volume of 60µl (Omega Biotek Inc., Norcross, GA). DNA samples were quantified using Qubit fluorometer (ThermoFisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... Plasma insulin was assessed using high sensitivity mouse insulin ELISA kit (Toronto Bioscience, Cat# 32270) and measured with a Synergy H4 Hybrid reader (Biotek Instruments). Area under the curve (AUC ...
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bioRxiv - Zoology 2020Quote: ... we extracted DNA from the well-preserved core of one fecal pellet per sample (Menke, Meier, & Sommer, 2015) using the E.Z.N.A.® DNA isolation kit (Omega Biotek, Norcross, GA, U.S.). The DNA concentration and quality were evaluated using Nanodrop™ 2000 Spectrophotometer (Nanodrop ...
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bioRxiv - Microbiology 2019Quote: ... was done according to the procedures in a commercial kit (Nanjing Jiancheng Biotechnology Institute, JiangSu, China) using microplate reader (Synergy 2, BioTek, USA).
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bioRxiv - Physiology 2021Quote: ... Plasma insulin levels were assessed using high sensitivity mouse insulin ELISA kit (Toronto Bioscience, Cat# 32270) and measured with a Synergy H4 Hybrid reader (Biotek Instruments). Area under the curve (AUC ...
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bioRxiv - Molecular Biology 2020Quote: ... Absorbance at the wavelengths specified in the kit manuals was determined using a BioTek Eon Microplate Spectrophotometer (BioTek, Winooski, VT, USA).
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bioRxiv - Microbiology 2022Quote: DNA was extracted from pure cultures using the Omega Mag-Bind Universal Pathogen Kit according to manufacturer’s protocol with a final elution volume of 60µl (Omega Biotek Inc., Norcross, GA). DNA samples were quantified using a Qubit fluorometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: Total microbial genomic DNA was extracted from cecum content samples using the E.Z.N.A.® soil DNA Kit (Omega Biotek, Norcross, GA, U.S.) according to the manufacturer’s instructions ...