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Citations for Biotek :
1 - 50 of 74 citations for Protein Arginine Methyltransferase 8 PRMT8 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and incubated in the Biospa 8 (Biotek) at 37°C and 80% humidity ...
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bioRxiv - Microbiology 2022Quote: ... OD600 measurements were taken on a BioSpa 8 plate reader (BioTek), and experiments were repeated at least three times ...
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bioRxiv - Cancer Biology 2022Quote: ... Plates were loaded into a BioSpa 8 Automated Incubator (BioTek Instruments). Time-lapse microscopy images were obtained for bright field ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated at 37°C with 5% CO2 using the Biospa 8 Automated Incubator (Biotek). Gen5 software (Biotek ...
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bioRxiv - Cancer Biology 2023Quote: ... Supernatant protein concentrations were quantified using a BCA Protein Assay kit (Pierce) and a spectrophotometric plate reader (BioTek). Most protein lysates were separated by running 30-100 μg of lysate on 10% Bis-Tris NuPage gels (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Spheroids were visualized at day 0 and day 8 post-treatment by using Cytation 5 imaging reader (BioTek) and ImageJ was used to process the areas of spheroids.
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bioRxiv - Microbiology 2023Quote: ... and protein concentrations determined using a Bicinchoninic Acid Assay (Pierce BCA Protein Assay, ThermoScientific) on an Eon spectrophotometer (BioTek). Total protein lysates were separated via SDS-PAGE and normalized across the isolates using Stain-FreeTM (BioRad ...
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bioRxiv - Immunology 2021Quote: ... The next day plates were washed 4 × in Tris Buffered Saline Tween (TBST-20mM Tris pH 8, 150mM NaCl, 0.1% Tween) using a plate washer (BioTek) and blocked with Casein (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... The next day plates were washed 4 × in Tris Buffered Saline Tween (TBST-20mM Tris pH 8, 150mM NaCl, 0.1% Tween) using a plate washer (BioTek) and blocked with Casein (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... Single strain growth assays were performed three times using 96-well plates by taking OD600 measurements on a Tecan Infinite 200 PRO plate reader or BioSpa 8 (BioTek). Tolerance experiments were performed by exposing exponentially growing bacteria to ∼10xMIC of an antibiotic ...
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bioRxiv - Synthetic Biology 2022Quote: ... 8 hour kinetics were performed at 37°C with either a Cytation 3 a Synergy HTX plate reader (Biotek Instruments) using excitation/emission settings of 485 nm and 528 nm ...
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bioRxiv - Bioengineering 2022Quote: ... Pellet was then resuspended in 1 mL of M9 medium and flasks were inoculated to the starting OD600 of 0.1 and incubated at 30 °C with agitation (233 RPM for cultures with substrate concentration 4 g/L and 275 RPM for cultures with substrate concentration 8 g/L) using NB205 orbital incubator (N-Biotek). The ratio of medium to flask volume was 1:5 (20 mL:100 mL ...
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bioRxiv - Cell Biology 2022Quote: ... Scratches were imaged immediately after the addition of CM (0 hours) and after 8 and 24 hours incubation using the automated Cytation 1 Imaging Reader at 4X (BioTek, with Gen5 Version 3.04 software ...
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bioRxiv - Synthetic Biology 2023Quote: Fluorescence microscopy was performed with Cytation 5 Cell Imaging Multimode Reader (Bio-Tek) equipped with a BioSpa 8 Automated Microplate Incubator (BioTek). A 20× objective was used to collect bright-field images (autofocusing auto exposure) ...
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bioRxiv - Biophysics 2023Quote: ... The bottom of the attractant reservoir was imaged for migrated cells for 8 h with a Cytation 5 BioSpa (Biotek) at 37 °C with 5 % CO2 ...
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bioRxiv - Genomics 2020Quote: ... SQ RNA/DNA/Protein Extraction Kit (Omega Biotek Inc.). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... using an excitation wavelength of 330/80 nm and emission wavelengths at 620/10 nm and 665/8 nm via Biotek IP1 dual filter cubes (Biotek, 8040505) using the following settings ...
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bioRxiv - Cell Biology 2021Quote: ... 1 µl/ml HaloTag NanoBRET 618 ligand was added to the cell suspension and cells were seeded at a concentration of 8 x103 cells per well of a 384-well plate in quadruplicate (BioTek, Winooski, VT), with the remaining cells plated into a fresh 6-well tissue culture plate ...
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bioRxiv - Neuroscience 2020Quote: ... The neurons were transferred to an automated live-cell incubator/imaging system (BioSpa™ 8 Automated Incubator coupled with a Cytation™ 5 Cell Imaging Multi-Mode Reader, BioTek, USA). The plates were incubated in the BioSpa at 37°C and 5% CO2 for 4 days ...
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bioRxiv - Cell Biology 2020Quote: ... neurons were stored in an automated incubator/imaging system (Cytation™ 5 Cell Imaging Multi-Mode Reader associated with a BioSpa™ 8 Automated Incubator, BioTek, USA). The plates were stored in the BioSpa at 37°C and 5% CO2 and were automatically transferred to the Cytation™ 5 for imaging ...
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bioRxiv - Molecular Biology 2023Quote: ... and a 16 h light / 8 h dark cycling regime with constant shaking (100 RPM, orbital shaker; N-Biotek, Bucheon, Republic of Korea). The cell density was measured using an automated cell counter (TC-20 ...
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bioRxiv - Cell Biology 2020Quote: Total protein levels were calibrated using Bicinchoninic Acid (BCA) assay (ThermoFisher Pierce BCA Protein Assay kit, OD measured with BioTek synergy 2 multi-mode multiplate reader). Samples were ran on a 9% SDS polyacrylamide gel ...
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bioRxiv - Physiology 2023Quote: ... Protein concentration in the host fraction was measured on a spectrophotometer (BioTek PowerWave XS2 ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration was quantified using a Synergy H1 microplate reader (BioTek Instruments, Inc.) with a Take3 Micro-Volume Plate (BioTek Instruments ...
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bioRxiv - Evolutionary Biology 2019Quote: ... SOD was expressed as units/mg/ml protein (quantified using a Biotek Take3 protocol). Red blood cells were lysed as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by the addition of protein in Alpha Buffer using the EL460 dispenser (BioTek). After 15 min of incubation ...
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bioRxiv - Physiology 2022Quote: ... protein concentration was determined by absorbance at 450 nm in a plate reader (Biotek Instrument).
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bioRxiv - Immunology 2020Quote: ... Protein quantification was carried out after thawing in a Synergy HTX Multi-Mode Reader (Biotek).
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bioRxiv - Plant Biology 2022Quote: ... with a BSA range and protein concentration was quantified with a ELx808 microplate reader (Biotek). Enzymatic activity was measured via the derived products generated from a 4-MUG substrate (M1404 ...
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bioRxiv - Immunology 2021Quote: ... Protein content was measured using 260/280 absorbance ratio with Take3 Micro-Volume Plate reader (BioTek)
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration was estimated using a Synergy H1 microplate reader (BioTek Instruments, Inc., Winooski, VT, USA) with a Take3 Micro-Volume Plate (BioTek Instruments ...
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bioRxiv - Biochemistry 2021Quote: ... The absorbance of the concentrated protein was taken at 280 nm using Synergy H4 Hybrid Reader (BioTek) on a Take3 plate ...
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bioRxiv - Bioengineering 2024Quote: ... Approximately 20 ug of protein from each sample was resolved on a 4-20% gradient gel (Biotek), then visualized by SimplyBlue SafeStain.
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bioRxiv - Immunology 2021Quote: ... Concentrations of antigens were determined using standard curves constructed with purified recombinant proteins and calculated with Gen5 3.02.2 (BioTek).
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bioRxiv - Cell Biology 2022Quote: ... 0.5 mM DTT) into the protein mix using the automated dispenser of a Synergy 4 microplate reader (BioTek) to a final volume of 200 μl and a final concentration of 2 μM actin in 1x KMEI with 0.05% antifoam ...
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bioRxiv - Bioengineering 2020Quote: ... Reactions for the fluorescent proteins were carried out in a Synergy Neo2 HTS Multi-Mode Microplate Reader (BioTek) at 282 cpm.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein quantification of exosome and HCQ@ZnS@exo@iRGD were done by using Bradford assay (BioTek, Winooski, USA). The stability of the vesicles was studied by monitoring the change in size and PDI over a 7-day period.
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bioRxiv - Immunology 2020Quote: ... unspecific protein binding sites were blocked by incubating sections with PBS containing 10% normal donkey serum (Pan-Biotek), 2% BSA and 0.2% Triton-X100 for 1 hour ...
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bioRxiv - Biophysics 2022Quote: In vivo and in vitro spectra of all proteins were recorded using Synergy H4 Hybrid Microplate Reader (BioTek) with resolution of 9 nm ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total protein was quantified by absorbance at wavelength 280 nm using a Take3 microvolume plate (BioTek Synergy HT) and 1 μg was boiled for 10 min in 1x Laemmli Sample Buffer (BioRad ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total protein was quantified by absorbance at wavelength 280 nm using a Take3 microvolume plate (BioTek Synergy HT). In assay buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... and the total protein in each extract was estimated by reading the OD280nm using the spectrophotometer (Epoch, BioTek). For the Western blot ...
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bioRxiv - Biochemistry 2020Quote: ... Low-affinity complexes and unbound proteins were removed in consecutive washing steps with PBS-T 0.05% (EL406 washer, BioTek). Beads were incubated for 10 minutes with 0.4% paraformaldehyde ...
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bioRxiv - Plant Biology 2019Quote: ... and the fluorescence intensity of Venus-tagged protein was measured on a Synergy H4 Hybrid reader (Biotek, Winooskit, VT, USA) at excitation and emission wavelengths of 485 nm and 528 nm ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined by taking OD at 280 nm in Nano drop (Synergy H1 Hybrid Multi-Mode Reader Biotek) and mass extinction co-efficient of the proteins.
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bioRxiv - Neuroscience 2021Quote: ... The solubilized protein was quantified by BCA Assay Kit (Fisher) in triplicates at 590nm using a microplate reader (Synergy HT, BioTek). 15 to 20 µg of ß-mercaptoethanol denatured protein were loaded in Tris-glycine polyacrylamide gels (14% ...
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bioRxiv - Microbiology 2020Quote: The TreS activity domain of TreS-Mak protein was monitored following the conversion of trehalose into maltose and glucose using Epoch spectrophotometer (Biotek). 15µL of reaction sample were incubated 30 min at 58°C with 30 µL of 100 mM sodium citrate pH 4.6 containing whether 0.4 U of amyloglucosidase from Aspergillus niger (Megazyme ...
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bioRxiv - Neuroscience 2020Quote: ... All assays were allowed to incubate at 37°C for 30 min before protein concentration was read in at 562 nm (SynergyMix, Biotek).
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bioRxiv - Cancer Biology 2022Quote: ... Protein quantification was performed using the Pierce BCA protein assay kit (ThermoScientific) and analyzed on a Cytation 3 plate reader (BioTek). Proteins were separated by NuPAGE 4-12% Bis-Tris Gel and transferred to a nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell and tissue debris was removed by centrifugation at 16,000x g for 5 minutes at room temperature and protein concentration was measured using A280 values determined by Cytation 5 (BioTek). Lysates were normalized in Buffer A with 1% SDS and prepared with 6X Laemmli sample buffer containing 100 mM 2-mercaptoethanol (Sigma M6250) ...