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Citations for Biotek :
301 - 341 of 341 citations for L Leucine 3 4 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... supernatant or size exclusion purified vesicles were extracted with butanol (1:2) overnight at 4°C and the butanol fraction transferred to microtiter plates for absorbance measurement (Synergy, Biotek).
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bioRxiv - Biochemistry 2020Quote: ... 30 μL of a 10 nM solution of FphF in PBS/0.01%TritonX-100 was added and fluorescence (λex = 365 nm and λem = 455 nm) was read at 37°C in 1 min intervals on a Cytation 3 imaging reader (BioTek, Winooski, VT, USA) for 60 min ...
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bioRxiv - Cell Biology 2022Quote: ... The reduction of resazurin to resorufin was measured fluorometrically at 540 nm excitation and 590 nm emission in a Cytation 3 microplate reader (BioTek Instruments Inc., USA). The results were expressed as % of untreated cells ...
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bioRxiv - Microbiology 2020Quote: ... Em = 450 nm) over a period of 30-60 minutes at 37°C on a Cytation-3 imaging reader (BioTek, Winooski, VT, USA). Concentrations of hydrolyzed products were determined using a standard concentration curve (unconjugated 4MU) ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were incubated at 37°C and fluorescence was measured (Ex = 410 nm, Em = 450 nm) over a period of 60 minutes on a Cytation 3 imaging reader (BioTek, Winooski, VT, USA). Reaction rates were determined by linear regression analysis of the initial linear phase of hydrolysis ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Relative dopaminergic cell viability was assessed by counting MAP2- and TH-positive neurons in a blinded manner using images taken with an automated Cytation 3 Cell Imaging Reader (BioTek Instruments, Winooski, VT) equipped with a 4X objective ...
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bioRxiv - Microbiology 2023Quote: ... and inhibitor simultaneously Cleavage was measured using fluorescence (EX 380 nm/EM 460 nm) with Cytation 3 imaging reader (BioTek, Winooski, VT, USA) for 60 minutes and slope was used to calculate the inhibition curve ...
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bioRxiv - Synthetic Biology 2023Quote: ... 80 µL ONE-Glo EX Reagent was then added and the plate was orbitally shaken at 300 rpm for 3 min in a microplate reader (BioTek Synergy H1, v2.04.11). Firefly luminescence was collected using the microplate reader’s luminescence fiber ...
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bioRxiv - Microbiology 2019Quote: ... clear bottom 96-well plate and absorbance was measured at 425 nm in a Biotek Synergy 4 plate reader (BioTek, USA). A standard curve was made with bisdemethoxycurcumin standards dissolved in oleyl alcohol (Figure S3)
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bioRxiv - Molecular Biology 2021Quote: ... The kinetics of substrate cleavage (increase in fluorescence of the liberated 7-amino-4-methylcoumarin, AMC) was measured using a fluorometer (BioTek, USA) set at 370 nm excitation and 460 nm emission at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Endpoint titers were determined by 4-parameter analysis using the Gen5™ 2.0 Data Analysis software (BioTek Instruments, Inc., Winooski, VT) and defined as the reciprocal of the serum dilution producing a signal identical to that of negative control samples at the same dilution plus three times the standard deviation.
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bioRxiv - Cancer Biology 2021Quote: ... was added to each well for 3 h and the absorbance at 450 nm was measured on an Epoch™ Microplate Spectrophotometer (Biotek, Winooski, VT, USA). Cytotoxicity data were expressed as mean ± standard derivation (SD ...
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bioRxiv - Neuroscience 2019Quote: ... Experiments with CSF samples and neurons on 96-well plates were acquired using a Biotek Cytation 3 Imaging Reader (BioTek Instruments, Winooski, VT, USA) equipped with a 20× ...
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bioRxiv - Immunology 2019Quote: ... The plate absorbance was measured by reading the plates at 450 nm using a Multi Mode reader (BioTek™ Cytation™ 3; ThermoFischer Scientific).
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bioRxiv - Bioengineering 2023Quote: ... We affixed the balloons to a glass microscope slide using tape and acquired images at 4x magnification with a Cytation 3 Image reader (BioTek Instruments Inc, Winooski, VT). All images were exported using a 16-bit resolution (65536 grey levels ...
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bioRxiv - Cell Biology 2024Quote: ... 100 µL of Tris (pH 10) was added and optical density was measured at 540 nm in Biotek Cytation 3 reader (Biotek Instruments, Winooski, VT, USA) and analyzed using the Wave Desktop 2.6.1 software (RRID:SCR_014526).
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bioRxiv - Neuroscience 2021Quote: ... Uptake of MEs labeled with FM 4-64 and RNA cargos labeled with ExoGlow-RNATM was quantified by using a microplate fluorescence reader (BioTek Instruments, Inc.) and confocal microscopy imaging respectively ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 11.1 μl of Alamar blue reagent was added and the readings were taken after 4 hours in a Synergy H1 plate reader (BioTek, Winooski, VT) with 560 nm (excitation ...
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bioRxiv - Microbiology 2021Quote: ... 4 and 5 were performed using two plate readers: A Synergy Neo2 and a Synergy H1 (both from Biotek Inc., VT, USA). Both were set to 30°C with continuous shaking at an orbital displacement of 1 mm and a speed of 807 rpm ...
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bioRxiv - Cell Biology 2022Quote: ... and changes in intracellular Ca2+ concentrations were measured by recording the ratio of fluorescence intensities at 508/20 nm (15 second time interval) using a Synergy 4 multimode microplate reader (BioTek, Winooski, VT). Traces were normalized to their respective baseline intracellular Ca2+ level obtained by averaging the 340/11 nm/380/20 nm ratios of each experiment in the absence of stimulus ...
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bioRxiv - Microbiology 2022Quote: ... The plate was incubated with shaking at 37 °C and the optical density of each well was measured at a wavelength of 600 nm (BIOTEK, Synergy 4). The MIC values were taken as the lowest concentration for which no growth was discernible (<0.05 OD600 ...
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bioRxiv - Microbiology 2023Quote: ... The resulting 1,472 SICs were arranged in 16 96-well plates and incubated in the anaerobic chamber at 37 °C (by setting the temperature of the chamber to 37 °C) in a plate stacker (BioTek BioStack 4) with 2 control plates containing uninoculated media at the top and bottom of the stack to minimize edge effects ...
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bioRxiv - Neuroscience 2023Quote: ... After 40 hours, cells were fixed in 4% PFA and images (eGFP, mCherry and eBFP2) were acquired at cytationC10 (Biotek, Objective 60x). eGFP (interaction signal ...
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bioRxiv - Microbiology 2023Quote: ... was recorded every 1 min over a period of 4 hours at 37°C using the Cytation 5 microplate reader (BioTek Instruments, USA).
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bioRxiv - Synthetic Biology 2023Quote: ... The absorbance at 405 nm was measured every 1 min for at least 30 min and up to 2 h using a Synergy 4 microplate reader (Biotek, Winooski, VT). SEAP activities were calculated as a function of the increase in absorbance at 405 nm over time42.
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bioRxiv - Neuroscience 2023Quote: ... and fluorescence emitted by Atto488 (λex = 485 nm, λem = 538 nm) was measured in a Synergy 4 plate reader (BioTek Instruments GmbH) at intervals of 10 seconds ...
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bioRxiv - Microbiology 2024Quote: ... NBT was reduced to blueformazan and spectrophotometrically read at OD 650 nm using a Synergy 4 microplate reader (BioTek, Santa Clara, USA). The concentration at which the drugs were able to inhibit 50% parasite growth (IC50 ...
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bioRxiv - Bioengineering 2020Quote: ... 5% CO2 using an automated digital microscope with a 10× objective with a gas controller (Cytation 3 microplate reader, BioTek Instruments Inc., Winooski, VT, USA). Images were collected every 10 minutes for 24h ...
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bioRxiv - Cancer Biology 2020Quote: ... was added and intensities were measured for each well using a Cytation 3 Multi Modal Reader with Gen5 software (BioTek Instruments, Inc., Winooski, VT, USA). Along with cell viability ...
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bioRxiv - Cell Biology 2022Quote: ... We also performed immunofluorescence staining for pluripotency markers in these iPSC colonies using Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit (Thermo Fischer Scientific) as per manufacturer’s instruction and fluorescence were imaged using fluorescence microscopy (BioTek Lionheart LX Automated Microscope) as described previously.15 Alkaline phosphatase (ALP ...
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bioRxiv - Cell Biology 2021Quote: ... Absorbances were obtained at 620 nm and sample concentrations were determined by comparison to the CXCL12 standards using a 4-parameter curve fit (Synergy HT & Gen5 Software, BioTek Instruments, Winooski, VT). Serum level of CXCL12 was assessed using undiluted serum isolated from the blood of tumor-bearing mice or stage IV glioma patients.
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bioRxiv - Evolutionary Biology 2022Quote: ... and Abs750nm of all 42 plates was measured after 65h exposition (4-6 hours after light on) with an absorbance microplate reader Epoch (BioTek Instruments, VT, USA). 9 data points per well were analyzed and averaged using software Gen5 2.0 (Biotek ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μL of substrates was added and fluorescence (λex◻=◻365◻nm and λem◻=◻455◻nm) was read at 37°C in 1 min intervals on a Cytation 3 imaging reader (BioTek, Winooski, VT, USA) for 60◻min ...
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bioRxiv - Cell Biology 2023Quote: ... the fluorescence was measured at 340 nm excitation wavelength and 480 nm emission wavelength in a Cytation™ 3 microplate reader (BioTek Instruments Inc., Winooski, VT, USA).
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bioRxiv - Cell Biology 2023Quote: ... The fluorescent signal was measured at 548 nm excitation wavelength and 574 nm emission wavelength in a Cytation™ 3 microplate reader (BioTek Instruments Inc., Winooski, VT, USA). As a positive control ...
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bioRxiv - Molecular Biology 2019Quote: ... The plate was incubated in the dark at room temperature for 45 minutes and subsequently read at 1 sec integrated reads in a luminescent plate reader (Biotek Synergy 4 plate reader).
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bioRxiv - Microbiology 2023Quote: ... We performed PCA oxidation assays either by tracking the fluorescence of reduced PCA over time in a plate reader (BioTek Synergy 4 or HTX) housed in a Coy-brand anoxic chamber or by tracking the current generated by a culture when provided PCA and an electrode poised to continuously reduce any PCA that the cells oxidized ...
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bioRxiv - Molecular Biology 2024Quote: ... 2-4 images at the central callus and distal callus regions were taken using an automatic microscope (Agilent, BioTek Lionheart FX, Santa Clara, CA). Central callus regions were defined as proximal to the fracture site on either side of the bone ...
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bioRxiv - Immunology 2022Quote: ... Reactions were stopped by adding 50 μL 3M hydrochloric acid and absorbance at 492 nm was determined on a Synergy 4 plate reader (BioTek, Agilent Technologies inc., CA, USA) or similar ...
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bioRxiv - Microbiology 2020Quote: ... UC500) and growth at 37 °C was monitored by measuring optical density at 600 nm every 15 minutes (Biotek Eon instrument with a BioStack 4). Carbon sources tested include D-glucose ...
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bioRxiv - Biochemistry 2023Quote: The polymerization and depolymerization of pyrene–labeled recombinant WT or mutant actin (5% pyrene-labeled WT or mutant actin) was monitored as a function of increasing or decreasing pyrene– fluorescence using a Synergy 4™ microplate reader (BioTek Instruments, Winooski, VT, USA) and the built–in filter set (Excitation ...