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Citations for Biotek :
151 - 200 of 214 citations for IL 3 Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Luminescence values were read using a multi-well luminometer (Cytation 3 Cell Imaging Multi•Mode Reader, BioTek Instruments, Winooski, Vermont).
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bioRxiv - Bioengineering 2020Quote: ... Well plates were always protected from light and fluorescence measurements were carried out at 37°C and 5% CO2 at Cytation 3 (Cell Imaging Multi-Mode Reader) from Biotek® (USA) ...
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bioRxiv - Microbiology 2021Quote: ... The number of cells in each well was quantified based on Hoechst 33342 staining detected by Cytation 3 imaging reader (BioTek). Image analysis was performed using ImageJ and customized R scripts ...
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bioRxiv - Microbiology 2022Quote: All bacteria were incubated anaerobically in the 24-well plate at 37°C for 3 hours in an Epoch 2 Microplate Spectrophotometer (BioTek), with optical density measured at 600 nm (OD600) ...
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bioRxiv - Microbiology 2022Quote: ... the bacteria were first incubated in 3 wells of a 96-well plate for 1 hour in an Epoch 2 Microplate Spectrophotometer (BioTek), with optical density measured at 600 nm (OD600) ...
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bioRxiv - Microbiology 2022Quote: ... Anaerobic growth was monitored by measuring absorbance (OD600) every 10 min for 24 h at 37 °C using a Cytation 3 plate reader (BioTek).
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bioRxiv - Microbiology 2022Quote: ... 355/460 nm for ACC substrates) was measured every minute for 30 min at 37 °C on a Cytation 3 plate reader (BioTek). Initial velocities were quantified by calculating the slope of the linear phase of the reaction as relative fluorescent units (RFUs ...
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bioRxiv - Molecular Biology 2022Quote: ... Absorbance at 360 nm was measured after 15 minutes using a 96-well plate using a plate reader (Cytation 3, BioTek).
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bioRxiv - Immunology 2022Quote: ... Wells were washed gently 3 times with white-RPMI and relative fluorescence was measured with Synergy H1 microplate reader (Biotek). For phagocytosis quantification in organoids ...
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bioRxiv - Neuroscience 2022Quote: ... Cy3 dye signal in the respective input cell substrates and eluted products that result from the magnetic-based IP was analyzed in a CytationTM 3 automated fluorescence plate reader (BioTek).
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bioRxiv - Neuroscience 2023Quote: ... Fluorescence emission (528, 590 nm) was measured over the course of 3 hours using a Synergy 2 microplate reader (Biotek) with excitation at 485 nm.
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bioRxiv - Biochemistry 2023Quote: ... 250 μl of DPPH solution was mixed with 10 μl of the extract (3 mg/ml) and measured at 517 nm using UV/Vis microplate spectrophotometer (BioTek, Model Epoch2TC ...
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bioRxiv - Cancer Biology 2023Quote: ... incubated for 4 h and fluorescence was read at 550 nm/590 nm on a Cytation 3 Imaging Reader (BioTek).
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bioRxiv - Cancer Biology 2023Quote: ... The absorbance at 412 nm was measured every 15 sec for 5 min on a Cytation 3 Imaging Reader (BioTek). Total concentration of GSH + GSSG was calculated based on a GSH standard curve.
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bioRxiv - Biophysics 2023Quote: ... The absorbance at 550nm was recorded every 40 seconds for 3 hours at room temperature while shaking (using the Double Orbital Continuous shaker in the BioTek Plate Reader ...
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bioRxiv - Microbiology 2023Quote: ... The OD600 was measured at 37°C every 3 minutes for 1 hour with continuous linear shaking in a Synergy H1 microplate reader (Biotek). All germinant stocks solutions were solubilized in water and filter sterilized ...
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bioRxiv - Microbiology 2023Quote: Luciferase assay experiments were performed in a 96-well format and measured in a 96-well white opaque plate using a Cytation 3 plate reader (BioTek) or a TriStar2 S microplate reader (Bertold Technologies) ...
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bioRxiv - Genetics 2024Quote: ... The fluorescence intensity (excitation = 570 nm, emission = 615 nm) of the plate was read immediately with Cytation 3 Multi-mode Reader (BioTek) at 25° C for 4 hours every 3 minutes.
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bioRxiv - Cancer Biology 2020Quote: ... Magnetic cell sorting was performed to separate purified human versus mouse cell fractions using the Mouse Cell Depletion Kit (Miltenyi Biotek, 130-104-694). For the proteomic experiments ...
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bioRxiv - Cell Biology 2022Quote: ... Plates were incubated at room temperature under agitation for 30 min and luminescence representing the number of viable cells was quantified with a Cytation 3 imaging reader (BioTek®).
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bioRxiv - Immunology 2021Quote: ... Fluorescence was measured (ex = 370 nm/em = 460 nm) for 1 hour with a Cytation 3 imaging reader (BioTek, Winooski, VT). Changes in fluorescence were converted to thrombin concentrations using a calibration curve created from sequential dilutions of human thrombin ...
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bioRxiv - Molecular Biology 2020Quote: ... The effect of pyocin S8 on the growth was monitored by measuring the turbidity of the cultures every 10 min for 3 h using the automated Sinergy™ H1 absorbance microplate reader (BioTek).
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bioRxiv - Molecular Biology 2020Quote: ... The resultant colour was measured at 410 nm using a plate reader spectrophotometer (Cytation 3 Imaging Reader, BioTek, Winooski, VT, USA). A standard curve of triglycerides ranging from 0 to 60 μg was run independently and in parallel with the experimental samples ...
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bioRxiv - Bioengineering 2022Quote: Luminescence was measured on a plate reader (BioTek Synergy™ HTX for collecting data presented in Figures 1, 3, 5, S5, S8, S12, S14; BioTek Synergy™ Neo2 for Figures 2C-D ...
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bioRxiv - Bioengineering 2022Quote: ... The fluorescence intensity of crosslinked collagen was measured at 420 nm emission/340nm excitation using a multimode microplate reader (Cytation 3, BioTek, USA).[17] The fluorescence intensity for each crosslinked collagen was measured in triplicate at 24-h intervals over 3 days before being averaged and then compared to the control.
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bioRxiv - Biochemistry 2021Quote: ... Fluorescence was measured (ex = 370 nm/em = 460 nm) for 1 hour with a Cytation 3 imaging reader (BioTek, Winooski, VT). Changes in fluorescence were converted to thrombin concentrations using a calibration curve created from sequential dilutions of human thrombin ...
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bioRxiv - Genetics 2022Quote: ... Brightfield and GFP images were taken every 3 hours for 30 hours using the Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments). GFP quantification was performed by a Cell Profiler program (https://cellprofiler.org/) ...
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bioRxiv - Microbiology 2019Quote: ... The percentage of growth inhibition in each well was determined by measuring optical density at 600nm (OD600) in a Cytation 3 Imaging plate Reader (BioTek Instruments) and comparing it to a well with no drug ...
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bioRxiv - Synthetic Biology 2019Quote: ... The absorbance reading was done at 340 nm every 5 seconds for 3 minutes in a BioTek PowerWave XS microplate reader (Biotek, USA). For NADH at 340 nm 6.22 mM cm-1 extinction coefficient (εM ...
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bioRxiv - Synthetic Biology 2022Quote: ... and the luminescence levels and optical density at 600 nm (OD600) of the cell cultures were simultaneously measured over time using a Cytation 3 microplate reader (BioTek, USA). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plate was incubated for 30 min at 22 °C and the luminescent signal was measured in a Cytation™ 3 (BioTek) multifunctional microplate reader ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Two hours later the wells were washed three times with HBSS and fluorescence was read at 340/505 and 380/505 on a Cytation™ 3 (BioTek) multifunctional microplate reader ...
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bioRxiv - Cancer Biology 2023Quote: ... The spheroids were grown for 21 days and imaged every 7 days with Cytation 3 Imaging reader (Biotek, Winooski, VT, USA). ImageJ software was used to measure the lengths and areas of the spheroids to compare the formed spheroids over time ...
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bioRxiv - Biophysics 2024Quote: ... Samples were shaken at 25ºC for 10 min and read for absorbance at 595 nm using a plate reader (Biotek Cytation 3). Protein concentration was calculated based on calibration curves generated in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... Absorbance values at 600 nm were measured at 5-minute intervals over 21 hours at specified temperature with orbital shaking using a Cytation 3 Plate Reader (Biotek; Winooski, VT). Doubling time was calculated by determining the OD600 values to an exponential curve as using a custom Matlab code as described previously (Fees and Moore 2018) ...
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bioRxiv - Immunology 2021Quote: ... Cell migration was assessed by a fluorescence (excitation at 485nm, emission at 530nm) plate reader Cytation 3 Cell Imaging Multi-Mode Reader (Biotek Instruments, Inc). The actual chemotaxis values were obtained by subtracting random chemotaxis values (PBS ...
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bioRxiv - Microbiology 2019Quote: ... The luminescence was regularly determined from culture samples using a multi-mode microplate reader (Cytation 3; BioTek Instruments, Inc., Winooski, VT, USA) and expressed in relative light units per optical density of the culture (RLU/OD600) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2 h later the fluorescence was read at 340/505 and 380/505 on a Cytation™ 3 (BioTek (Vermont, MA, USA)) multifunctional microplate reader ...
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bioRxiv - Cancer Biology 2021Quote: ... was added to each well and the absorbance was measured at 340nM for the duration of the experiment by a multi-mode imaging reader (BioTek, Cytation 3).
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bioRxiv - Microbiology 2020Quote: ... Fluorescence (Ex = 335 nm and Em = 450 nm) was measured every minute on a Cytation 3 imaging reader (BioTek, Winooski, VT, USA) for 60 min ...
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bioRxiv - Bioengineering 2023Quote: ... It was then incubated for 1 hour at room temperature before the absorbance was read at 570 nm using a microplate reader (BioTek, Cytation 3). The manufacturer’s standard protocol was used to calibrate the measurements.
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bioRxiv - Synthetic Biology 2023Quote: ... The plate was shaken at 300 rpm (orbital) for 3 min and BRET biosensor performance was recorded using the plate reader’s luminescence filter (BioTek Synergy H1, v2.04.11) with an integration time of 1 s ...
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bioRxiv - Microbiology 2024Quote: ... Quantification of the extracted DNA was conducted spectrophotometrically with the Take 3 plate in an Eon plate reader/incubator (BioTek, Winooski, VT).
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bioRxiv - Microbiology 2024Quote: ... Fluorescence microscopy images (4X and 40X magnification) were acquired with the Cytation™ 3 Cell Imaging Multi-Mode Reader (BioTek® Instruments). Macrophage-residing HIV-1 in VCCs was analyzed with 40X magnification images ...
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bioRxiv - Biochemistry 2020Quote: ... 30 μL of a 10 nM solution of FphF in PBS/0.01%TritonX-100 was added and fluorescence (λex = 365 nm and λem = 455 nm) was read at 37°C in 1 min intervals on a Cytation 3 imaging reader (BioTek, Winooski, VT, USA) for 60 min ...
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bioRxiv - Cell Biology 2022Quote: ... The reduction of resazurin to resorufin was measured fluorometrically at 540 nm excitation and 590 nm emission in a Cytation 3 microplate reader (BioTek Instruments Inc., USA). The results were expressed as % of untreated cells ...
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bioRxiv - Microbiology 2020Quote: ... Em = 450 nm) over a period of 30-60 minutes at 37°C on a Cytation-3 imaging reader (BioTek, Winooski, VT, USA). Concentrations of hydrolyzed products were determined using a standard concentration curve (unconjugated 4MU) ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were incubated at 37°C and fluorescence was measured (Ex = 410 nm, Em = 450 nm) over a period of 60 minutes on a Cytation 3 imaging reader (BioTek, Winooski, VT, USA). Reaction rates were determined by linear regression analysis of the initial linear phase of hydrolysis ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Relative dopaminergic cell viability was assessed by counting MAP2- and TH-positive neurons in a blinded manner using images taken with an automated Cytation 3 Cell Imaging Reader (BioTek Instruments, Winooski, VT) equipped with a 4X objective ...
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bioRxiv - Microbiology 2023Quote: ... and inhibitor simultaneously Cleavage was measured using fluorescence (EX 380 nm/EM 460 nm) with Cytation 3 imaging reader (BioTek, Winooski, VT, USA) for 60 minutes and slope was used to calculate the inhibition curve ...