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Citations for Biotek :
351 - 400 of 453 citations for Glutamate Decarboxylase 2 GAD2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... by pipetting 250 µL and using a Biotek® Epoch™ 2 multi-well plate reader (Biotek Instruments, Winooski, VT).
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bioRxiv - Molecular Biology 2023Quote: Fluorescence polarization experiments were carried out at 25 °C in PBS supplemented by 2 mM TCEP and 0.05% Brij using a Synergy H4 (BioTek Instruments) plate reader ...
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bioRxiv - Microbiology 2023Quote: ... The absorbance at λ420nm was measured every minute for 1-2 h to follow the kinetics of ONPG hydrolysis at 28 °C in a Synergy H1 plate reader (BioTek). The velocity of the reaction was calculated by performing linear regression using GraphPad Prism software ...
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bioRxiv - Microbiology 2023Quote: ... supernatant or size exclusion purified vesicles were extracted with butanol (1:2) overnight at 4°C and the butanol fraction transferred to microtiter plates for absorbance measurement (Synergy, Biotek).
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bioRxiv - Cancer Biology 2023Quote: ... Assay plates were measured for the formazan product at 490 nM using a Synergy 2 plate reader (BioTek, Winooski, VT). Net lactate production was determined by subtracting lactate in the initial media from cancer cell’s lactate production at each time point during the experiments.
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bioRxiv - Microbiology 2023Quote: ... then diluted to A600=0.1-0.001 into either 5-10mL broth or into 96-well plates (Costar), and growth was monitored by measuring A600 every hour (broth culture) or every 15min (Epoch 2 or SynergyMx, (Biotek) plate readers. ...
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bioRxiv - Microbiology 2023Quote: ... Culture plates were incubated at 37°C for 22 hrs and kept anaerobic during the OD600 measurement in a Synergy 2 Plate Reader (Biotek Agilent Technologies Deutschland GmbH ...
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bioRxiv - Neuroscience 2023Quote: ... Forty microliters of the lysate was transferred to a black 96-well plate for a luminescent reading on a Synergy 2 (BioTek) or a GloMax (Promega).
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bioRxiv - Cell Biology 2024Quote: ... The mixture was allowed to equilibrate for 2 hr in the dark and the FP signal was measured by Synergy NEO HTS multi-mode microplate reader (Biotek). The background FP signal was measured by supplementing the reaction with 10 mM EDTA ...
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bioRxiv - Pathology 2023Quote: ... 100 µmol/L) were added and the formation of thrombin monitored over 10 minutes using a fluorescence plate reader (Synergy 2, BioTek Instruments ...
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bioRxiv - Neuroscience 2024Quote: ... The increase of absorbance was measured at 412 nm using Multimode microplate reader Synergy 2 (BioTek Inc., Winooski, VT, USA). For the calculation of the resulting measured activity (the percentage of inhibition I ...
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bioRxiv - Microbiology 2023Quote: The rapidity by which tailocins killed bacteria was measured in 96-well plates (Nest) using a Epoch 2 microplate reader (BioTek). Twenty-five µl of a suspension containing tailocins (approximately 1011 particles mL-1 ...
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bioRxiv - Immunology 2022Quote: ... Antibody levels are presented as optical density absorbance values at 450 nm (BioTek ELISA plate reader).
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bioRxiv - Immunology 2019Quote: ... Recovered mAb-F(ab’)2 sample were evaluated for purity by SDS-PAGE and their concentration measured by Take5 (BioTek instruments).
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bioRxiv - Molecular Biology 2021Quote: ... followed by detection of the absorbance at 420 and 550 nm at every 2 min using the Viento Nano microplate reader (BioTek Instruments). The relative LacZ activity was calculated as follows ...
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bioRxiv - Physiology 2020Quote: ... The samples were incubated 37 °C for 2 hours and absorbance was measured at 562 nm using a microplate spectrophotometer (BioTek Epoch).
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bioRxiv - Biochemistry 2021Quote: ... The released pNP (extinction coefficient ε=16,000 M-1cm-1) was measured at 405 nm using Epoch 2 plate reader (Biotek, US) set to 37°C and subtracting the blank reading ...
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bioRxiv - Microbiology 2020Quote: ... Promoter activity was then followed by continuous measurement of the growth and GFP fluorescence (one measure every 30 min during 10 h) of the cultures with the Synergy™2 Multi-detection microplate reader (Biotek). Promoter activity was then estimated by dividing the fluorescence value by the OD600 value for each time point.
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bioRxiv - Physiology 2021Quote: ... A 1.5 h kinetics curve was monitored at 37° using a Synergy 2 multi-mode microplate reader (BioTek, Winooski, VT, USA). The absolute amount of cleaved substrate was calculated by an AMC standard curve ...
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bioRxiv - Immunology 2021Quote: ... The reaction was stopped with 50 µL of 2N sulfuric acid and the absorbance at 450 nm was read with an Epoch 2 microplate reader (Biotek, USA).
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bioRxiv - Microbiology 2019Quote: ... was done according to the procedures in a commercial kit (Nanjing Jiancheng Biotechnology Institute, JiangSu, China) using microplate reader (Synergy 2, BioTek, USA).
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bioRxiv - Biophysics 2019Quote: ... and growth was monitored by measuring the OD600 every 5 minutes in a Biotek Synergy 2 plate reader (Biotek Instruments, inc.). It was found that the 80-fold dilution was the highest concentration of buffer that did not inhibit growth of S ...
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bioRxiv - Neuroscience 2021Quote: ... Purified total RNA was quantified on a Synergy 2 Multimode Microplate Reader using a Take3 micro-volume plate (BioTek, Winooski, VT). For cDNA synthesis ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... DNA concentration and purity was monitored by electrophoresis on 2% (w/v) agarose gels and spectrophotometrically by determination of A260/A280 ratios (Synergy HT Microplate Reader, BioTek, USA). Amplicon based metagenomics was performed using the NEBNext® Ultra™ IIDNA Library Prep Kit (Cat No ...
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bioRxiv - Immunology 2020Quote: ... The reaction was stopped with stop solution and absorbance was read at 450 nm using Epoch 2 microplate reader (BioTek, USA). Data are expressed as stimulation index (S.I ...
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bioRxiv - Neuroscience 2021Quote: ... Relative Light Units (RLU) were measured at 28°C using the Synergy 2 SL Microplate Reader with Gen5™ software (BioTek).
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bioRxiv - Biochemistry 2023Quote: ... Fluorescence was measured for 2 h at excitation and emission on a Synergy HTX Multi-Mode Microplate Reader (BioTek, Winooski, VT).
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bioRxiv - Microbiology 2023Quote: ... The cultures were grown under strong agitation at 37°C and analyzed in Synergy 2 Multi-mode microplate reader (BioTek Instruments). Relative Luminescence Units (RLU ...
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bioRxiv - Microbiology 2023Quote: ... transferred to a 96-well plate and incubated at 30°C with double-orbital shaking at 355 cpm in an Epoch 2 microplate reader (BioTek, Germany). Growth was monitored by measuring the OD600 at 10-min intervals until the stationary phase was reached ...
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bioRxiv - Microbiology 2024Quote: ... Free phosphate released upon hydrolysis of the TDP or UDP produced by RmlT was quantified by absorbance at 620 nm using a Synergy 2 multi-mode plate reader (BioTek, USA). RmlT reaction mixtures were also separated in 20 % polyacrylamide gels and WTA were stained with 1 mg mL-1 Alcian Blue solution after overnight incubation.
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bioRxiv - Microbiology 2024Quote: ... Cells were washed three times with PBS and binding to the bacterial cell wall was visualized using a fluorescence microscope Olympus BX53 or quantified using a plate reader Synergy 2 (BioTek, USA).
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bioRxiv - Cancer Biology 2023Quote: ... Total protein quantification was assayed using the EZQ Protein Quantification Kit (Thermo R33200) according to the manufacturer’s protocol and results were read on a fluorescence-based microplate reader (BioTek Synergy 2). Click chemistry of the biotin-alkyne (PEG4 carboxamide-propargyl biotin ...
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bioRxiv - Bioengineering 2020Quote: ... the perfusate from both the fetal and maternal channels was collected and its fluorescence intensity quantified utilizing a microplate reader (BioTek Synergy 2).
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bioRxiv - Evolutionary Biology 2022Quote: ... Chlorophyll a and b were extracted using an acetone and alcohol protocol(Zhao et al., 2018) and quantified using Synergy™2 multi-detection microplate reader (Biotek, VT). Light response curve was measured based on the red and blue light sources using Li 6800 (Li-COR ...
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bioRxiv - Molecular Biology 2021Quote: Fluorescence intensities were measured by a Cary Eclipse spectrofluorometer (Varian, Palo Alto, CA) for single time point measurements and a Synergy 2 plate reader (Biotek, Winooski, VT) for multiple time point measurements.
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bioRxiv - Biochemistry 2019Quote: ... Chemiluminescence was measured every 1-2 minutes over 1.5 hours at 37 °C using the Synergy H1 multi-detection microplate reader (Biotek, Bad Friedrichshall, Germany) in triplicates and chemiluminescence activity was expressed as integrated total counts as calculated by the addition of rectangles with unit width under individual data points ...
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bioRxiv - Physiology 2019Quote: ... and an aliquot of the synthesized RNA probes were then visually assessed using standard agarose gel electrophoresis and quantified on a SYNERGY 2 Microplate reader (Biotek, Winooski, VT).
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bioRxiv - Physiology 2019Quote: ... The final purified PCR products for use as templates for RNA probe synthesis were quantified on a SYNERGY 2 Microplate reader (Biotek, Winooski, VT).
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bioRxiv - Physiology 2019Quote: ... aegypti CAPAr were loaded into each well of multi-well plate using an automated injector module linked to a Synergy 2 Multi Mode Microplate Reader (BioTek, Winooski, VT) which measured kinetic luminescent response from each well for 20 sec immediately following cell loading onto the different peptides at various doses ...
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bioRxiv - Microbiology 2020Quote: ... OD600 recordings were taken every 30 minutes for 44 hours and then every 60 minutes for 96 hours (6 days total) with an Epoch 2 Microplate Spectrophotometer (Biotek, Winooski, VT) at 45°C within a rigid gloveless hypoxic chamber (Coy Lab ...
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bioRxiv - Microbiology 2020Quote: Pure cultures of defined mutants were diluted to A600 0.003 and grown +/− antibiotic in 96-well microtiter format at 37°C with shaking in a plate reader (Tecan M200 Pro, Biotek Epoch 2, or Biotek Synergy H2M). Growth was monitored as change in A600 ...
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bioRxiv - Microbiology 2021Quote: ... The 96-well plates were incubated anaerobically in a temperature-controlled plate reader at 37°C (Epoch 2 Microplate Spectrophotometer, BioTek, Swindon, UK). For each test and technical replicate ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2 h later the fluorescence was read at 340/505 and 380/505 on a Cytation™ 3 (BioTek (Vermont, MA, USA)) multifunctional microplate reader ...
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bioRxiv - Biochemistry 2020Quote: ... Ten parallel bacterial cultures of 100 μL for each condition were shaken in a Synergy 2 microplate reader (BioTek, Winooski, VT, USA) at 37 oC ...
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bioRxiv - Bioengineering 2021Quote: ... Then the plate was vortexed at 400 rpm for 2 min and luminescence read on a Synergy Neo2 plate reader (BioTek, Winooski, VT).
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bioRxiv - Microbiology 2021Quote: ... The OD600 was used to monitor growth during incubation at 30 °C for 48 h without aeration using a Synergy 2 microplate reader (Biotek, Winooski, VT).
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bioRxiv - Biochemistry 2021Quote: ... and inhibitor (APPI monomer, 0-400 nM; APPI dimer, 0-96 nM) in a Synergy 2 Multi-Detection Microplate Reader (BioTek, VT, USA). Mesotrypsin buffer (296 μL) ...
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bioRxiv - Biochemistry 2021Quote: ... Fluorescent polarization was read parallel and perpendicular to the excitation plane (ex. 485 nm/ em. 528 nm) using a Synergy 2 Microplate reader (BioTek, Winooski, VT) and the relationship between signal and time was linear (Figure S2) ...
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bioRxiv - Cancer Biology 2020Quote: ... and the plate was returned to incubator for 2 hours after which absorbance was read at 450 nm in a microplate reader (BioTek Instruments Inc).
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bioRxiv - Microbiology 2020Quote: ... Serosal samples were collected every 30 minutes for 2 hours with FITC dextran flux (ng/ml/cm2/hr) determined by plate reader (Biotek Synergy H1).