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Citations for Biotek :
101 - 150 of 183 citations for Dengue Virus Subtype 4 Envelope 45 kDa Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The 96-well plates were incubated at 37°C for 16 hours using the BioTek synergy 4 (BioTek, USA) plate reader ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial growth was monitored once per hour for 24 h after 30 sec linear shaking (Eon + Biostack 4, Biotek) under anaerobic conditions ...
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bioRxiv - Microbiology 2019Quote: ... and optical density and fluorescence were monitored for 48 hours in a Biotek Synergy 4 plate reader (BioTek, USA) at 30 °C with fast continuous shaking ...
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bioRxiv - Microbiology 2020Quote: ... the plate was further incubated at room temperature for 4 minutes and read by a spectrophotometer (BioTek, VT, USA) using a 540nm filter.
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bioRxiv - Biochemistry 2021Quote: ... The absorbance of the concentrated protein was taken at 280 nm using Synergy H4 Hybrid Reader (BioTek) on a Take3 plate ...
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bioRxiv - Microbiology 2020Quote: ... was added to each well to stop the reaction and the optical density (OD) was measured at 492 nm on a Synergy 4 plate reader (BioTek). An average of OD values for blank wells plus three standard deviations was used to set a cutoff for plate blank outliers ...
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bioRxiv - Microbiology 2019Quote: ... The resazurin was diluted at 0.004% in regular D10 medium and incubated with host cells at 37°C with 5% CO2 for 4 h before absorbance measurement at 570 and 600 nm by BioTek H1 Hybrid plate reader ...
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bioRxiv - Microbiology 2019Quote: ... The plates were sealed with a semi-permeable film (Thermo Fischer) and incubated at 30°C in a Synergy 4 plate reader (BioTek) with shaking and absorbance readings (600 nm ...
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bioRxiv - Biochemistry 2019Quote: ... Enzyme velocity was determined by measuring the formation of the 4-nitrophenolate product of hydrolysis (405 nm) for four minutes at room temperature using an Epoch microplate spectrophotometer (BioTek). Initial rates were corrected for non-enzymatic hydrolysis and the Michaelis constant was determined by fitting the initial rates to the Michaelis-Menten equation using GraphPad Prism (Supplementary Fig ...
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bioRxiv - Cell Biology 2021Quote: ... Changes in intracellular Ca2+ concentrations were measured by recording the ratio of fluorescence intensities at 508/20 nm resulting from excitation of Fura-2 AM at 340/11 nm or 380/20 nm (center/bandpass) using a Synergy 4 multimode microplate reader (BioTek). Ratios were acquired every 0.7 seconds for 15 seconds before injection and 2 minutes after injection ...
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bioRxiv - Microbiology 2020Quote: ... and then optical density and fluorescence were monitored for 48 hours in an Biotek Synergy 4 plate reader (BioTek, USA) at 30 °C with fast continuous shaking ...
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bioRxiv - Cell Biology 2022Quote: ... Intracellular Fluo-4 AM was excited using automatic high-throughput living cell imaging and analysis system (BioTek, Lionheart FX, USA) at 494 nm ...
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bioRxiv - Synthetic Biology 2019Quote: ... and then optical density and fluorescence were monitored for 48 hours in a Biotek Synergy 4 plate reader (BioTek, USA) at 30 °C with fast continuous shaking ...
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bioRxiv - Synthetic Biology 2019Quote: ... and then optical density and fluorescence were monitored for 48□hours in an Biotek Synergy 4 plate reader (BioTek, USA) at 30□°C with fast continuous shaking ...
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bioRxiv - Biochemistry 2019Quote: ... Decrease in Cy5 fluorescence was monitored by measuring fluorescence at 680 nm by excitation at 635nm on a Synergy 4 plate reader (BioTek).
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bioRxiv - Biochemistry 2019Quote: Fluorescence anisotropy experiments were carried out at room temperature with 5 nM fluorescein (6FAM)-labelled DNA substrates (Table S2) using a Synergy 4 plate reader (BioTek). Titration with protein was performed by serially diluting protein in 40 μL of assay buffer (20 mM Tris Acetate pH 7.0 ...
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bioRxiv - Microbiology 2020Quote: ... the reaction was stopped with 50 μls of 3M hydrochloric acid and absorbance was measured at 490 nm (OD490) using a Synergy 4 (BioTek) plate reader ...
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bioRxiv - Microbiology 2022Quote: ... Fluorescence of released 4-methylumbelliferone (4MU) was measured (Excitation 360 nm, Emission 450 nm) in a plate reader (Synergy MX, Biotek). One unit of chitinase activity releases 1 μmol of 4MU from the appropriate substrate per minute ...
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bioRxiv - Bioengineering 2022Quote: ... Pellet was then resuspended in 1 mL of M9 medium and flasks were inoculated to the starting OD600 of 0.1 and incubated at 30 °C with agitation (233 RPM for cultures with substrate concentration 4 g/L and 275 RPM for cultures with substrate concentration 8 g/L) using NB205 orbital incubator (N-Biotek). The ratio of medium to flask volume was 1:5 (20 mL:100 mL ...
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bioRxiv - Microbiology 2022Quote: ... Plates were incubated at RT for 10 minutes in the dark before luminescence was measured using a Synergy 4 (BioTek) plate reader ...
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bioRxiv - Neuroscience 2023Quote: ... Absorbance at 490 nm was measured 4 hours after the addition of the MTS dye using a SynergyNEO2 plate reader (BioTek).
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bioRxiv - Physiology 2023Quote: ... The cells are first washed from their culture medium by 4 washes of KRB-BSA with the EL406 washer distributor liquid handling device (Biotek) suitable for 384-well plates ...
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bioRxiv - Cancer Biology 2023Quote: ... incubated for 4 h and fluorescence was read at 550 nm/590 nm on a Cytation 3 Imaging Reader (BioTek).
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bioRxiv - Microbiology 2023Quote: ... The reading process for multiple plates is automated using a BioStack™ 4 microplate stacker (BioTek Instruments, Inc., Winooski, VT), which cycles the experimental 96-well microplates and removes the lids at the specified interval as to not interfere with spectrophotometric values ...
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bioRxiv - Microbiology 2023Quote: ... supernatant or size exclusion purified vesicles were extracted with butanol (1:2) overnight at 4°C and the butanol fraction transferred to microtiter plates for absorbance measurement (Synergy, Biotek).
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bioRxiv - Immunology 2021Quote: ... Concentrations of antigens were determined using standard curves constructed with purified recombinant proteins and calculated with Gen5 3.02.2 (BioTek).
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bioRxiv - Bioengineering 2020Quote: ... Reactions for the fluorescent proteins were carried out in a Synergy Neo2 HTS Multi-Mode Microplate Reader (BioTek) at 282 cpm.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein quantification of exosome and HCQ@ZnS@exo@iRGD were done by using Bradford assay (BioTek, Winooski, USA). The stability of the vesicles was studied by monitoring the change in size and PDI over a 7-day period.
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bioRxiv - Immunology 2020Quote: ... unspecific protein binding sites were blocked by incubating sections with PBS containing 10% normal donkey serum (Pan-Biotek), 2% BSA and 0.2% Triton-X100 for 1 hour ...
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bioRxiv - Biophysics 2022Quote: In vivo and in vitro spectra of all proteins were recorded using Synergy H4 Hybrid Microplate Reader (BioTek) with resolution of 9 nm ...
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bioRxiv - Molecular Biology 2023Quote: ... and the total protein in each extract was estimated by reading the OD280nm using the spectrophotometer (Epoch, BioTek). For the Western blot ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total protein was quantified by absorbance at wavelength 280 nm using a Take3 microvolume plate (BioTek Synergy HT) and 1 μg was boiled for 10 min in 1x Laemmli Sample Buffer (BioRad ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total protein was quantified by absorbance at wavelength 280 nm using a Take3 microvolume plate (BioTek Synergy HT). In assay buffer ...
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bioRxiv - Microbiology 2019Quote: ... clear bottom 96-well plate and absorbance was measured at 425 nm in a Biotek Synergy 4 plate reader (BioTek, USA). A standard curve was made with bisdemethoxycurcumin standards dissolved in oleyl alcohol (Figure S3)
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bioRxiv - Molecular Biology 2021Quote: ... The kinetics of substrate cleavage (increase in fluorescence of the liberated 7-amino-4-methylcoumarin, AMC) was measured using a fluorometer (BioTek, USA) set at 370 nm excitation and 460 nm emission at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Endpoint titers were determined by 4-parameter analysis using the Gen5™ 2.0 Data Analysis software (BioTek Instruments, Inc., Winooski, VT) and defined as the reciprocal of the serum dilution producing a signal identical to that of negative control samples at the same dilution plus three times the standard deviation.
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bioRxiv - Biochemistry 2020Quote: ... Low-affinity complexes and unbound proteins were removed in consecutive washing steps with PBS-T 0.05% (EL406 washer, BioTek). Beads were incubated for 10 minutes with 0.4% paraformaldehyde ...
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bioRxiv - Neuroscience 2021Quote: ... Uptake of MEs labeled with FM 4-64 and RNA cargos labeled with ExoGlow-RNATM was quantified by using a microplate fluorescence reader (BioTek Instruments, Inc.) and confocal microscopy imaging respectively ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 11.1 μl of Alamar blue reagent was added and the readings were taken after 4 hours in a Synergy H1 plate reader (BioTek, Winooski, VT) with 560 nm (excitation ...
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bioRxiv - Microbiology 2021Quote: ... 4 and 5 were performed using two plate readers: A Synergy Neo2 and a Synergy H1 (both from Biotek Inc., VT, USA). Both were set to 30°C with continuous shaking at an orbital displacement of 1 mm and a speed of 807 rpm ...
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bioRxiv - Cell Biology 2022Quote: ... and changes in intracellular Ca2+ concentrations were measured by recording the ratio of fluorescence intensities at 508/20 nm (15 second time interval) using a Synergy 4 multimode microplate reader (BioTek, Winooski, VT). Traces were normalized to their respective baseline intracellular Ca2+ level obtained by averaging the 340/11 nm/380/20 nm ratios of each experiment in the absence of stimulus ...
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bioRxiv - Microbiology 2022Quote: ... The plate was incubated with shaking at 37 °C and the optical density of each well was measured at a wavelength of 600 nm (BIOTEK, Synergy 4). The MIC values were taken as the lowest concentration for which no growth was discernible (<0.05 OD600 ...
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bioRxiv - Microbiology 2023Quote: ... The resulting 1,472 SICs were arranged in 16 96-well plates and incubated in the anaerobic chamber at 37 °C (by setting the temperature of the chamber to 37 °C) in a plate stacker (BioTek BioStack 4) with 2 control plates containing uninoculated media at the top and bottom of the stack to minimize edge effects ...
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bioRxiv - Neuroscience 2023Quote: ... After 40 hours, cells were fixed in 4% PFA and images (eGFP, mCherry and eBFP2) were acquired at cytationC10 (Biotek, Objective 60x). eGFP (interaction signal ...
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bioRxiv - Microbiology 2023Quote: ... was recorded every 1 min over a period of 4 hours at 37°C using the Cytation 5 microplate reader (BioTek Instruments, USA).
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bioRxiv - Synthetic Biology 2023Quote: ... The absorbance at 405 nm was measured every 1 min for at least 30 min and up to 2 h using a Synergy 4 microplate reader (Biotek, Winooski, VT). SEAP activities were calculated as a function of the increase in absorbance at 405 nm over time42.
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bioRxiv - Neuroscience 2023Quote: ... and fluorescence emitted by Atto488 (λex = 485 nm, λem = 538 nm) was measured in a Synergy 4 plate reader (BioTek Instruments GmbH) at intervals of 10 seconds ...
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bioRxiv - Microbiology 2024Quote: ... NBT was reduced to blueformazan and spectrophotometrically read at OD 650 nm using a Synergy 4 microplate reader (BioTek, Santa Clara, USA). The concentration at which the drugs were able to inhibit 50% parasite growth (IC50 ...
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bioRxiv - Plant Biology 2019Quote: ... and the fluorescence intensity of Venus-tagged protein was measured on a Synergy H4 Hybrid reader (Biotek, Winooskit, VT, USA) at excitation and emission wavelengths of 485 nm and 528 nm ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined by taking OD at 280 nm in Nano drop (Synergy H1 Hybrid Multi-Mode Reader Biotek) and mass extinction co-efficient of the proteins.