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Citations for Biotek :
151 - 192 of 192 citations for DNA Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... RNA was extracted from frozen root material using the E.Z.N.A.® Plant RNA Kit with the associated RNase-free DNase I Set (Omega-Biotek, USA), following the standard protocol provided ...
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bioRxiv - Developmental Biology 2024Quote: ... and the band located at a 7.9 kb mark was cut and purified using the EZNA Cycle pure kit (Omega Biotek) Purified digested vector and inserts were ligated in a 1:30 vector/insert ratio using the T4 DNA ligase (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: ... the total RNA was extracted using the E.Z.N.A column-based total RNA kit (Omega BioTek, catalog number R6834-01) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... The solubilized protein was quantified by BCA Assay Kit (Fisher) in triplicates at 590nm using a microplate reader (Synergy HT, BioTek). 15 to 20 µg of ß-mercaptoethanol denatured protein were loaded in Tris-glycine polyacrylamide gels (14% ...
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bioRxiv - Neuroscience 2020Quote: ... The rats’ insulin levels were measured using an enzyme-linked immunosorbent assay (ELISA) kit (Hangzhou Eastbiopharm Co., Ltd., Hangzhou, China) and a spectrophotometer (Biotek Epoch™ Microplate Spectrophotometer ...
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bioRxiv - Microbiology 2020Quote: ... Each well was washed once with 1 mL 1X PBS and RNA was extracted using the EZNA Total RNA kit (Omega Biotek). 375 μL of TRK lysis buffer with β-Mercaptoethanol was added to each well and incubated for 3 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... or human IFN-β (1000 U/ml) and gaussia luciferase activity was determined using Gaussia Luciferase Glow Assay Kit (Thermo) on Cytation 3 (BioTek) multimode plate imager according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was isolated from clarified cell culture supernatants using an Omega Total RNA Isolation Kit 1 (Omega BioTek, VWR) and reverse transcribed at 42°C using an oligo dT primer and SuperScript II reverse transcriptase (Life Technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR products were purified by agarose gel and extracted using a GFX gel band extraction kit according to the manufacturer’s instruction (Omega Biotek, USA). 500ng of purified PCR fragments were used to prepare Digoxigenin or Fluorescein labelled antisense probes ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein quantification was performed using the Pierce BCA protein assay kit (ThermoScientific) and analyzed on a Cytation 3 plate reader (BioTek). Proteins were separated by NuPAGE 4-12% Bis-Tris Gel and transferred to a nitrocellulose membrane ...
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bioRxiv - Physiology 2021Quote: All serum insulin concentrations were quantified using a commercially available insulin immunoassay kit (32380; Toronto Bioscience) as per manufacturer’s instructions using Synergy H4 Hybrid microplate reader (BioTek Instruments). Insulin concentrations were interpolated from a standard curve determined by curve of 4-parameter as per manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: 72 hours after transfection cells were harvested and RNA extraction was performed using E.Z.N.A Total RNA Kit 1 (Omega Biotek cat #R6834-01). cDNA generation was performed using SuperScript IV Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... and lambda chains.48 The products from the second PCR were analyzed by agarose gel electrophoresis and purified PCR products (ENZA cycle pure kit; Omega Biotek) were submitted to Genewiz for sequencing ...
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bioRxiv - Plant Biology 2022Quote: ... Leaf tissue was flash frozen in liquid nitrogen and RNA extracted using the EZNA Plant RNA Kit I (Omega Biotek). DNA contamination was removed by treating total RNA with Turbo DNase with inactivation reagent (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA was isolated from frozen liver samples and DNase treated using commercially available kits (Omega Biotek and Ambion, respectively). Libraries were constructed and subsequently subjected to 151 bp paired-end cycles on the NovaSeq-6000 platform (Illumina) ...
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bioRxiv - Cell Biology 2022Quote: ... Lipid hydroperoxides were extracted from neuronal lysates and neuron-conditioned media and analyzed using a lipid hydroperoxide assay kit according to the manufacturers protocol and Synergy Mx Multi-Mode Microplate Reader (BioTek). VAMP7 and syntaxin 4 knockdown neurons used for the lipid hydroperoxide assay were transduced at an MOI 7.5.
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bioRxiv - Physiology 2023Quote: ... Soluble host protein of the supernatant was measured using the Pierce BCA Protein Assay Kit (Pierce Biotechnology, Waltham, Massachusetts, Catalog #23225) with two duplicates per sample measured by a 96 well microplate reader (BioTek Synergy HTX Multi-Mode Reader Model S1LFA ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA concentration measurements were performed using the Qubit dsDNA high sensitivity assay kit on the Synergy H1 Hybrid Microplate Reader (BioTek). Libraries were generated using the Nextera XT Library Prep kit (Illumina ...
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bioRxiv - Molecular Biology 2019Quote: ... All kits were performed according to manufacturer's instructions and plates were read using a 96-well spectrophotometer (BioTek, Winooski, VT, USA). Due to missed time points and one subject demonstrating exceptionally high PRE serum CK activity values ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from pure cultures using the Omega Mag-Bind Universal Pathogen Kit according to manufacturer’s protocol with a final elution volume of 60µl (Omega Biotek Inc., Norcross, GA). DNA samples were quantified using Qubit fluorometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from pure cultures using the Omega Mag-Bind Universal Pathogen Kit according to manufacturer’s protocol with a final elution volume of 60μl (Omega Biotek Inc., Norcross, GA). DNA samples were quantified using Qubit fluorometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from pure cultures using the Omega Mag-Bind Universal Pathogen Kit according to manufacturer’s protocol with a final elution volume of 60μl (Omega Biotek Inc., Norcross, GA). DNA samples were quantified using Qubit fluorometer (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: Total RNA extraction from H4 cells was carried out using the E.Z.N.A.® Total RNA Kit I (Omega Biotek, Norcross, GA, USA), and either purity (260nm/280nm ratio and 260nm/230nm ratio ...
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bioRxiv - Plant Biology 2020Quote: ... and total RNA was extracted using the protocol for difficult samples in E.Z.N.A.® Plant RNA Kits (Omega Biotek, Norcross, GA, USA). The samples were reverse transcribed and specific sequences were amplified using the SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... the firefly LUC and Renilla LUC activities were measured by Dual Luciferase Reporter Gene Assay Kit (Yeasen, Shanghai) and Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, USA). Activity is expressed as the ratio of firefly LUC activity to Renilla LUC activity ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from pure cultures using the Omega Mag-Bind Universal Pathogen Kit according to manufacturer’s protocol with a final elution volume of 60µl (Omega Biotek Inc., Norcross, GA). DNA samples were quantified using Qubit fluorometer (ThermoFisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... Plasma insulin was assessed using high sensitivity mouse insulin ELISA kit (Toronto Bioscience, Cat# 32270) and measured with a Synergy H4 Hybrid reader (Biotek Instruments). Area under the curve (AUC ...
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bioRxiv - Microbiology 2019Quote: ... was done according to the procedures in a commercial kit (Nanjing Jiancheng Biotechnology Institute, JiangSu, China) using microplate reader (Synergy 2, BioTek, USA).
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bioRxiv - Physiology 2021Quote: ... Plasma insulin levels were assessed using high sensitivity mouse insulin ELISA kit (Toronto Bioscience, Cat# 32270) and measured with a Synergy H4 Hybrid reader (Biotek Instruments). Area under the curve (AUC ...
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bioRxiv - Molecular Biology 2020Quote: ... Absorbance at the wavelengths specified in the kit manuals was determined using a BioTek Eon Microplate Spectrophotometer (BioTek, Winooski, VT, USA).
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bioRxiv - Microbiology 2022Quote: DNA was extracted from pure cultures using the Omega Mag-Bind Universal Pathogen Kit according to manufacturer’s protocol with a final elution volume of 60µl (Omega Biotek Inc., Norcross, GA). DNA samples were quantified using a Qubit fluorometer (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Total protein quantification was assayed using the EZQ Protein Quantification Kit (Thermo R33200) according to the manufacturer’s protocol and results were read on a fluorescence-based microplate reader (BioTek Synergy 2). Click chemistry of the biotin-alkyne (PEG4 carboxamide-propargyl biotin ...
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bioRxiv - Immunology 2021Quote: ... Secondary antibody binding was quantified using the Pierce TMB Substrate Kit (Thermo, Cat. 34021) and Synergy HT plate reader (BioTek, Winooski, VT). Antibody binding data were analyzed by nonlinear regression (One site specific binding with Hill slope ...
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bioRxiv - Microbiology 2021Quote: ... The quantity of DNA in each sample was fluorescently measured with the Quant-iT PicoGreen dsDNA Assay Kit (ThermoFicher, Cat# P11496) using a Synergy H1 Microplate reader (BioTek, Cat # BTH1M).
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bioRxiv - Microbiology 2022Quote: ... The quantity of DNA in each sample was measured with the Quant-iT PicoGreen dsDNA Assay Kit (ThermoFicher, Cat# P11496) read with a Synergy H1 Microplate reader (BioTek, Cat # BTH1M). Sequence libraries were prepared from the extracted DNA samples using the Nextera DNA Flex Library Prep Kit (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... high g pellet and high g supernatant was measured using an iron assay kit and Synergy Mx Multi-Mode Microplate Reader (BioTek Instruments Inc.).
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bioRxiv - Cell Biology 2022Quote: ... high g pellet and high g supernatant using a lipid hydroperoxide assay kit and Synergy Mx Multi-Mode Microplate Reader (BioTek Instruments Inc.). For the iron depletion assay ...
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ANGPTL8 R59W variant influences inflammation through modulating NF-κB pathway under TNFα stimulationbioRxiv - Biochemistry 2023Quote: ... The cell lysates were mixed with Luciferase reagent II (supplied in the kit) and was measured on Synergy Hybrid H4 plate reader (BioTek, Winooski, VT) and normalized according to protein concentration ...
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bioRxiv - Cell Biology 2022Quote: ... We also performed immunofluorescence staining for pluripotency markers in these iPSC colonies using Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit (Thermo Fischer Scientific) as per manufacturer’s instruction and fluorescence were imaged using fluorescence microscopy (BioTek Lionheart LX Automated Microscope) as described previously.15 Alkaline phosphatase (ALP ...
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bioRxiv - Cancer Biology 2020Quote: ... Magnetic cell sorting was performed to separate purified human versus mouse cell fractions using the Mouse Cell Depletion Kit (Miltenyi Biotek, 130-104-694). For the proteomic experiments ...
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bioRxiv - Systems Biology 2022Quote: ... The fluorogenic substrate provided with the kit was added and the plate was read for 30 minutes using a fluorometer (Biotek Synergy H1 microplate reader) at Ex:380/EM:460 nm.
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bioRxiv - Cell Biology 2020Quote: Total protein levels were calibrated using Bicinchoninic Acid (BCA) assay (ThermoFisher Pierce BCA Protein Assay kit, OD measured with BioTek synergy 2 multi-mode multiplate reader). Samples were ran on a 9% SDS polyacrylamide gel ...