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Citations for Biotek :
551 - 600 of 612 citations for 7 Benzyl 2 oxa 7 aza spiro4.4nonan 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and the cell plate was sat in dark for 1 h before absorbance at 517 nm was recorded using Cytation 5 Cell Imaging Multi-Mode Reader (BioTek). All values were normalized to the background ...
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bioRxiv - Microbiology 2020Quote: ... The DNA concentration and integrity were determined by electrophoresis on 1% agarose gels containing ethidium bromide and spectrophotometrically using an EPOCH instrument (BioTek). After confirmation ...
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bioRxiv - Biochemistry 2019Quote: ... 1 μg of total RNA (purity/integrity assessed by agarose gel electrophoresis and concentration determined by A260nm using a BioTek Cytation3 microplate reader with a Take3 accessory ...
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bioRxiv - Cancer Biology 2020Quote: ... The number of cells was determined at the end of the run using the SRB assay as previously described (62) or in plate cell counting by Cytation 1 imaging reader (Biotek).
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bioRxiv - Synthetic Biology 2021Quote: 72 hours after transfection cells were harvested and RNA extraction was performed using E.Z.N.A Total RNA Kit 1 (Omega Biotek cat #R6834-01). cDNA generation was performed using SuperScript IV Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... Luminescence was measured for around 70 h with 1 h intervals at a constant temperature of 25°C using a Synergy Mx Monochromator (Biotek).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Luminescence at 485 nm and fluorescent eYFP emission at 530 nm were measured for 1 second per well using a Cytation 5 plate Reader (BioTek). The ratio of eYFP/RLuc was calculated per well and data are presented as percent of dopamine response ...
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bioRxiv - Molecular Biology 2019Quote: ... Reactions were incubated for 1-2 min at room temperature and then absorbance measured at 412 nm for 10 min with 1 min interval using a Powerwave plate spectrophotometer (BioTek). The rate of change in absorbance per minute is linearly proportional to the total concentration of GSH ...
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bioRxiv - Immunology 2021Quote: ... after 1-5 minutes of adding TMB and absorbance at 450 nm was measured with a Synergy HTX plate reader (BioTek). Concentrations of antigens were determined using standard curves constructed with purified recombinant proteins and calculated with Gen5 3.02.2 (BioTek).
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bioRxiv - Microbiology 2022Quote: ... luminescence was quantified with an integration time of 1 s using the Cytation 5 Cell Imaging Multi-Mode Reader (BioTek).
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bioRxiv - Cell Biology 2022Quote: ... Scratches were imaged immediately after the addition of CM (0 hours) and after 8 and 24 hours incubation using the automated Cytation 1 Imaging Reader at 4X (BioTek, with Gen5 Version 3.04 software ...
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bioRxiv - Microbiology 2023Quote: ... 20 µl of 50 µg ml-1 ethidium bromide was added to each well and the absorbance was measured using the SynergyTM Mx microplate reader (BioTek) at 500 nm excitation and 580 nm emission wavelengths for 50 min.
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bioRxiv - Immunology 2022Quote: ... Reactions were quenched by addition of 1 N HCl and absorbance at 450 nm were analyzed using a plate reader (BioTek). ELISAs with gp120s and anti-idiotype monoclonal antibodies were performed as above except these proteins were immobilized directly onto high-binding 96-well assay plates (Costar ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were incubated with the reagent for at least 1 minutes prior to reading on Synergy H1 Hybrid Multi-Mode Reader (BioTek) or FLUOstar Omega Microplate Reader (BMG LABTECH) ...
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bioRxiv - Cell Biology 2023Quote: ... The absorbance at 360 nm was measured every 1 min over 45 min at 37°C by using a microplate reader (Synergy H1; BioTek). The detailed protocol was deposited in protocols.io (DOI ...
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bioRxiv - Cancer Biology 2023Quote: ... six wells for each shRNA were stained with 1 μg/mL Hoechst in PBS for 30 minutes and imaged using the Cytation 5 (Biotek) at 4X magnification taking six fields per well ...
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bioRxiv - Cell Biology 2023Quote: ... the exact number of nematode in each well was determined on a Cell Imaging Multi-Mode Reader (Cytation 1, BioTek) and was used for normalising/correcting OCR results.
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bioRxiv - Microbiology 2023Quote: ... The OD600 was measured at 37°C every 3 minutes for 1 hour with continuous linear shaking in a Synergy H1 microplate reader (Biotek). All germinant stocks solutions were solubilized in water and filter sterilized ...
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bioRxiv - Cancer Biology 2023Quote: ... the reaction was stopped with NaOH (10 μl, 1 M) and absorbance at 410 nm was recorded using Synergy H1 hybrid multi-mode reader (BioTek). The value of each group measured at day 0 was set as 1.
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bioRxiv - Physiology 2023Quote: ... the exact number of nematode in each well was determined on a Cell Imaging Multi-Mode Reader (Cytation 1, BioTek) and was used for normalising/correcting OCR results ...
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bioRxiv - Physiology 2023Quote: ... the exact number of nematode in each well was determined on a Cell Imaging Multi-Mode Reader (Cytation 1, BioTek) and was used for normalising/correcting OCR results ...
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bioRxiv - Immunology 2024Quote: ... samples were qualitatively assessed using the Take3 plate on the Cytation 1 cell imaging multi-mode reader with Gen5 software (BioTek). Samples which met established quality guidelines set by the sequencing facility were sent to University of Texas Genomic Sequencing and Analysis Facility for TagSeq library prep and sequencing on NovaSeq with single end ...
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bioRxiv - Immunology 2021Quote: ... Fluorescence was measured (ex = 370 nm/em = 460 nm) for 1 hour with a Cytation 3 imaging reader (BioTek, Winooski, VT). Changes in fluorescence were converted to thrombin concentrations using a calibration curve created from sequential dilutions of human thrombin ...
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bioRxiv - Synthetic Biology 2020Quote: ... and incubated at 37 °C (with a 1 °C gradient to minimize condensation) while shaking at 807 double-orbital cycles per minute in a plate reader (BioTek, Neo2SM). Optical density at 600 nm (OD600 ...
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bioRxiv - Plant Biology 2021Quote: ... 100 μL of supernatant were added to 100 μL of 1 N NaOH and the absorbance of the solution was measured at 440 nm using an Epoch microplate spectrophotometer (BioTek, USA).
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bioRxiv - Cell Biology 2020Quote: ... was measured at 37°C from 1 to 10 min using a fluorescence microtiter plate reader (Synergy HTX Multi-Mode Reader, BioTek, USA). eNOS activity was expressed as the VEGFA-dependent increase in fluorescence per μg of cellular protein ...
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bioRxiv - Bioengineering 2022Quote: Luminescence was measured on a plate reader (BioTek Synergy™ HTX for collecting data presented in Figures 1, 3, 5, S5, S8, S12, S14; BioTek Synergy™ Neo2 for Figures 2C-D ...
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bioRxiv - Biochemistry 2021Quote: ... Fluorescence was measured (ex = 370 nm/em = 460 nm) for 1 hour with a Cytation 3 imaging reader (BioTek, Winooski, VT). Changes in fluorescence were converted to thrombin concentrations using a calibration curve created from sequential dilutions of human thrombin ...
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bioRxiv - Microbiology 2020Quote: ... The reaction was followed by measuring the absorbance at 405 nm at 1-minute intervals for 10 minutes using a Microplate reader (BioTek, USA).
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bioRxiv - Molecular Biology 2021Quote: ... the samples’ absorbance was measured at 494 nm using a 1 cm pathlength cuvette and a plate reader spectrophotometer (PowerWave XS, BioTek Instruments). No free fluorophore was detected in the cassettes that had only AF488 (without collagen) ...
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bioRxiv - Microbiology 2022Quote: ... intracellular NR dye was extracted using a destaining solution (50% ethanol, 1% acetic acid and water) and absorbance was measured at 540 nm using a spectrophotometer (Biotek, Elx800). Percentage of inhibition of each drug was calculated normalizing data against the negative control absorbance (100% value).
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bioRxiv - Bioengineering 2022Quote: ... or four 75% washes (for 96-well plate) with starvation media (DMEM +1% P/S) using an automated plate washer (BioTEK ELx405). Experiments were performed 3-4 hours post-starvation ...
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bioRxiv - Physiology 2024Quote: ... live cells stained with Hoechst 33342 (1:2,000 dilution) (ThermoScientific, Cat#62249) were enumerated using Cytation 5 plate reader (BioTek Instruments - Agilent). Values obtained from the Mito-Stress test were normalized per 1,000 cells.
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bioRxiv - Cancer Biology 2024Quote: Serum insulin and insulin like growth factor 1 (IGF1) levels were measured by ELISA using a microplate reader (BioTek Instruments Inc.) and respective kits (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... Images of 2D-cultured RFP+ melanoma cells were captured using a Cytation 1 Cell Imaging Multi-Mode Reader (BioTek Instruments, Winooski, VT).
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bioRxiv - Bioengineering 2020Quote: ... luminescence was read immediately using a plate reader with a 1 second integration time (BioTek Synergy HT Plate Reader and Gen5 Software, BioTek Instruments, Inc.). Relative luminescence for each sample was calculated by subtracting the luminescence from the sample blank (cell medium ...
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bioRxiv - Microbiology 2020Quote: ... Samples were examined using an Olympus 1×81 spinning disk confocal microscope (Tokyo, Japan) or Cytation 5 Cell Imaging Multi-Mode Reader instrument (Biotek, Winooski, VT). Images were analyzed using Volocity or Gen5 software ...
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bioRxiv - Microbiology 2019Quote: ... Maximum specific growth rates (μmax) presented in Table 1 were assayed at 30 °C using a Synergy HTX multi-mode microplate reader (BioTek, Winooski, VT) using 48-well plates ...
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bioRxiv - Physiology 2023Quote: ... cells were imaged with 6 fields/well using a high content fluorescent image acquisition and analysis platform Cytation 1 (BioTek, VT, USA) [37 ...
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bioRxiv - Bioengineering 2023Quote: ... It was then incubated for 1 hour at room temperature before the absorbance was read at 570 nm using a microplate reader (BioTek, Cytation 3). The manufacturer’s standard protocol was used to calibrate the measurements.
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bioRxiv - Microbiology 2023Quote: ... was recorded every 1 min over a period of 4 hours at 37°C using the Cytation 5 microplate reader (BioTek Instruments, USA).
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bioRxiv - Physiology 2023Quote: ... was incubated with a working calcium color reagent for 1 min and the absorbance read at 575 nm using a spectrophotometer (BioTek Synergy HTX).
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bioRxiv - Biochemistry 2023Quote: ... Reactions were started by addition of 1 mM Mg-ATP and NADH absorbance was monitored at 340 nm in a plate reader (Biotek PowerWave HT) at 26°C ...
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bioRxiv - Cell Biology 2024Quote: Cell proliferation rates were measured with label-free high-contrast brightfield microscopy in 6-well plates using Cytation 1 Cell Imaging Multi-Mode Reader (BioTek Instruments, Agilent) as described previously 58 ...
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bioRxiv - Microbiology 2024Quote: ... The remaining crystal violet was fully dissolved in 1 ml 95% ethanol with constantly shaking and measured its optical density at 590 nm (Biotek microplate reader). The assay was repeated at least three times with similar results.
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bioRxiv - Microbiology 2024Quote: ... The growth behavior of the different mutants of AB5075-VUB was assessed in liquid culture using the Cytation 1 plate reader (BioTek, United States). Each well of a 96-well plate was filled with 200 µL of bacterial culture diluted to an initial OD600 of 0.1 ...
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bioRxiv - Immunology 2021Quote: ... and absorbance was measured at 450 nm using a plate reader after stopping the reaction with 50 μl of 1 N HCl (Biotek Epok, Model # 3296573).
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bioRxiv - Immunology 2021Quote: ... and absorbance was measured at 450 nm using a plate reader after stopping the reaction with 50 μl of 1 N HCl (Biotek Epok, Model # 3296573).
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bioRxiv - Biochemistry 2020Quote: ... 30 μL of a 10 nM solution of FphF in PBS/0.01%TritonX-100 was added and fluorescence (λex = 365 nm and λem = 455 nm) was read at 37°C in 1 min intervals on a Cytation 3 imaging reader (BioTek, Winooski, VT, USA) for 60 min ...
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bioRxiv - Cancer Biology 2021Quote: ... and the percentage of GFP+ CAFs or RFP+ melanoma cells was determined using a Cytation 1 Cell Imaging Multi-Mode Reader (BioTek Instruments, Winooski, VT). The efficiency of β-catenin ablation by shRNA in CAFs was determined by Western blotting.