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Citations for Biotek :
1 - 50 of 975 citations for 7 8 DIHYDRO 1 3 DIOXOLO 4 5 G ISOQUINOLIN 5 6H ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Pellet was then resuspended in 1 mL of M9 medium and flasks were inoculated to the starting OD600 of 0.1 and incubated at 30 °C with agitation (233 RPM for cultures with substrate concentration 4 g/L and 275 RPM for cultures with substrate concentration 8 g/L) using NB205 orbital incubator (N-Biotek). The ratio of medium to flask volume was 1:5 (20 mL:100 mL ...
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bioRxiv - Microbiology 2020Quote: ... and 3 dpi by high content imaging (BioTek Cytation 5) configured with brightfield and GFP cubes ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell and tissue debris was removed by centrifugation at 16,000x g for 5 minutes at room temperature and protein concentration was measured using A280 values determined by Cytation 5 (BioTek). Lysates were normalized in Buffer A with 1% SDS and prepared with 6X Laemmli sample buffer containing 100 mM 2-mercaptoethanol (Sigma M6250) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell debris was removed by centrifugation at 16,000x g for 5 minutes at room temperature and protein concentration was measured using A280 values determined by Cytation 5 (BioTek). Lysates were normalized in Buffer A with 1% SDS and prepared with 6X Laemmli sample buffer containing 100 mM 2-mercaptoethanol (Sigma M6250) ...
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bioRxiv - Immunology 2023Quote: ... The cells were incubated at 37°C and 5% CO2 in a Cytation 7 plate Imager (Biotek) and both brightfield and GFP images were collected every 30 minutes for 18 hours ...
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bioRxiv - Synthetic Biology 2024Quote: Incubate for 3 h and 5 min in a plate reader (Biotek Neo2SM).
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bioRxiv - Synthetic Biology 2024Quote: Incubate for 3 h and 5 min in a plate reader (Biotek Neo2SM).
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated at 37°C with 5% CO2 using the Biospa 8 Automated Incubator (Biotek). Gen5 software (Biotek ...
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bioRxiv - Microbiology 2022Quote: ... 5 (BioTek). OD600nm readings were taken every hour and growth curves were plotted using GraphPad Prism 9.
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bioRxiv - Bioengineering 2022Quote: Luminescence was measured on a plate reader (BioTek Synergy™ HTX for collecting data presented in Figures 1, 3, 5, S5, S8, S12, S14; BioTek Synergy™ Neo2 for Figures 2C-D ...
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bioRxiv - Cancer Biology 2023Quote: ... Spheroids were visualized at day 0 and day 8 post-treatment by using Cytation 5 imaging reader (BioTek) and ImageJ was used to process the areas of spheroids.
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bioRxiv - Microbiology 2023Quote: ... was recorded every 1 min over a period of 4 hours at 37°C using the Cytation 5 microplate reader (BioTek Instruments, USA).
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bioRxiv - Cell Biology 2023Quote: ... One measure of fluorescence (Ex/Em 560/590 nm) was then read each hour (h) using a Cytation 5 (BioTek) driven by the Gene 5.1.11 Software ...
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bioRxiv - Cell Biology 2021Quote: ... for 6h and measuring the absorbance at 595 nm with a spectrophotometer (BioTek Synergy).
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bioRxiv - Bioengineering 2022Quote: ... using Cytation 5 (Biotek). Maximal respiration was calculated by subtracting non-mitochondrial OCR from FCCP OCR ...
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bioRxiv - Synthetic Biology 2023Quote: Fluorescence microscopy was performed with Cytation 5 Cell Imaging Multimode Reader (Bio-Tek) equipped with a BioSpa 8 Automated Microplate Incubator (BioTek). A 20× objective was used to collect bright-field images (autofocusing auto exposure) ...
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bioRxiv - Biophysics 2023Quote: ... The bottom of the attractant reservoir was imaged for migrated cells for 8 h with a Cytation 5 BioSpa (Biotek) at 37 °C with 5 % CO2 ...
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bioRxiv - Developmental Biology 2022Quote: ... Automated cell counting was conducted using the Cytation 3 and Cytation 5 Cell Imaging Multi-Mode Readers (Biotek).
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bioRxiv - Bioengineering 2023Quote: ... Cytation 5 imaging reader (BioTek) was used to read the plate for absorbance at 450nm when the assay was complete.
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bioRxiv - Microbiology 2021Quote: ... Imaging was performed using a Cytation 5 multimode imaging system with Gen 5 software (Biotek).
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bioRxiv - Microbiology 2021Quote: ... Images were captured as a 5×4 matrix and GFP positive cells were enumerated using Gen5 software (Biotek).
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bioRxiv - Immunology 2022Quote: ... Fluorescence images of the entire well were acquired with a 4 X objective in a Cytation 5 (BioTek). The total number of cells indicated by the nuclei staining and the infected cells indicated by the NP staining were quantified with the cellular analysis module of the Gen5 software (BioTek) ...
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bioRxiv - Microbiology 2022Quote: ... The quality and yield of RNA were analyzed by the Take-3 Plate and Cytation 5 plate reader (BioTek). For the elimination of genomic DNA contamination ...
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bioRxiv - Synthetic Biology 2023Quote: ... was measured by exciting the samples with polarized light at 485/20 nm and emission at 528/20 nm at a parallel and perpendicular angle for 40 min with an interval of 5 min to make sure the plateau is maintained using a Synergy 4 plate reader (BioTek Synergy 4, Vermont, United States). Fluorescence polarization was then calculated using the following equation: Fǁ = fluorescence parallel to the excitation plane ...
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bioRxiv - Neuroscience 2020Quote: ... The neurons were transferred to an automated live-cell incubator/imaging system (BioSpa™ 8 Automated Incubator coupled with a Cytation™ 5 Cell Imaging Multi-Mode Reader, BioTek, USA). The plates were incubated in the BioSpa at 37°C and 5% CO2 for 4 days ...
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bioRxiv - Cell Biology 2020Quote: ... neurons were stored in an automated incubator/imaging system (Cytation™ 5 Cell Imaging Multi-Mode Reader associated with a BioSpa™ 8 Automated Incubator, BioTek, USA). The plates were stored in the BioSpa at 37°C and 5% CO2 and were automatically transferred to the Cytation™ 5 for imaging ...
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bioRxiv - Immunology 2023Quote: ... with a Cytation 5 reader (Biotek). A portion of the lysate was boiled at 95°C for 10 minutes in 2X Laemmli buffer to evaluate protein expression via immunoblot.
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bioRxiv - Cell Biology 2019Quote: ... a Lionheart FX automated microscope in combination with sirDNA77 staining was used to generate growth curves with a time resolution of 4 hours for a total time span of 136 hours (microscope maintained at 37°C, 5% CO2 using a 4× lens and a Sony CCD, 1.25 megapixel camera with 2 times binning; BioTek). Quantification of cell number was performed by Gen5 software (BioTek).
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bioRxiv - Bioengineering 2020Quote: ... Well plates were always protected from light and fluorescence measurements were carried out at 37°C and 5% CO2 at Cytation 3 (Cell Imaging Multi-Mode Reader) from Biotek® (USA) ...
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bioRxiv - Cancer Biology 2023Quote: ... The absorbance at 412 nm was measured every 15 sec for 5 min on a Cytation 3 Imaging Reader (BioTek). Total concentration of GSH + GSSG was calculated based on a GSH standard curve.
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bioRxiv - Microbiology 2019Quote: ... The resazurin was diluted at 0.004% in regular D10 medium and incubated with host cells at 37°C with 5% CO2 for 4 h before absorbance measurement at 570 and 600 nm by BioTek H1 Hybrid plate reader ...
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bioRxiv - Biochemistry 2019Quote: Fluorescence anisotropy experiments were carried out at room temperature with 5 nM fluorescein (6FAM)-labelled DNA substrates (Table S2) using a Synergy 4 plate reader (BioTek). Titration with protein was performed by serially diluting protein in 40 μL of assay buffer (20 mM Tris Acetate pH 7.0 ...
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bioRxiv - Genetics 2022Quote: ... Brightfield and GFP images were taken every 3 hours for 30 hours using the Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments). GFP quantification was performed by a Cell Profiler program (https://cellprofiler.org/) ...
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bioRxiv - Synthetic Biology 2019Quote: ... The absorbance reading was done at 340 nm every 5 seconds for 3 minutes in a BioTek PowerWave XS microplate reader (Biotek, USA). For NADH at 340 nm 6.22 mM cm-1 extinction coefficient (εM ...
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bioRxiv - Immunology 2021Quote: ... 5% CO2 and real-time monitoring of NK cell killing was performed on a Cytation(tm) 5 (Biotek). Cell images were processed using GEN5 software (Biotek) ...
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bioRxiv - Bioengineering 2021Quote: ... using a plate reader (BioTek Cytation 5). Experiments were done in triplicates.
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bioRxiv - Immunology 2021Quote: ... on the Cytation 5 Microplate Reader (BioTek), and cDNA size distribution was measured using the High-Sensitivity DNA Bioanalyzer Kit (Agilent ...
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bioRxiv - Biochemistry 2020Quote: ... or a Cytation 5 plate reader (Biotek), respectively.
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bioRxiv - Biochemistry 2020Quote: ... using a Cytation 5 reader (BioTek Instruments).
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bioRxiv - Neuroscience 2023Quote: ... using a 5 μL dispense cassette (BioTek) and incubated for at least 1 hour at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... Absorbance values at 600 nm were measured at 5-minute intervals over 21 hours at specified temperature with orbital shaking using a Cytation 3 Plate Reader (Biotek; Winooski, VT). Doubling time was calculated by determining the OD600 values to an exponential curve as using a custom Matlab code as described previously (Fees and Moore 2018) ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were initiated by adding 5 μl of 2 mM MUNANA and the fluorescence was measured on Cytation 5 (Biotek) plate reader at 37°C for 10 min using 30 sec intervals and a 365 nm excitation wavelength and a 450 nm emission wavelength ...
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bioRxiv - Immunology 2022Quote: ... Fluorescence was measured at Ex/Em: 485/535 nm recorded at 37°C every 5 min using Cytation 5 Cell Imaging Multi-Mode Reader (BioTek).
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bioRxiv - Microbiology 2022Quote: ... AM readings were performed by measuring fluorescence at 340/11 nm and 380/20 nm excitation and 508/20 emission every 20 seconds for 5 minutes using a Cytation 5 (BioTek). The solution was replaced with Ca2+-free Ringer’s solution containing either 2 μM thapsigargin (TG ...
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bioRxiv - Immunology 2022Quote: ... PI fluorescence was measured at Ex/Em: 535/617 nm at 37°C every 5 min using Cytation 5 Cell Imaging Multi-Mode Reader (BioTek). The obtained measurements were baseline corrected to DMSO at each timepoint and was presented as Ftreatment - FDMSO ...
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bioRxiv - Microbiology 2020Quote: ... LDH absorbance was read using Cytation 5 (BioTek) at manufacturer’s recommended excitation and emissions.
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bioRxiv - Bioengineering 2020Quote: ... on a Cytation 5 plate reader (BioTek Instruments) (37°C ...
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bioRxiv - Bioengineering 2020Quote: ... on a Cytation 5 plate reader (BioTek Instruments). The number of cells that had migrated within the scratch region were quantified using ImageJ software.
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bioRxiv - Bioengineering 2020Quote: ... on a Cytation 5 plate reader (BioTek Instruments) (37°C ...
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bioRxiv - Microbiology 2020Quote: ... using Cytation 5 cell imager (Biotek, Winooski, VT). Infection was normalized to control (0 µM drug ...