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Citations for Biotek :
101 - 148 of 148 citations for 6 Piperidino 1 purine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... the reaction was stopped with NaOH (10 μl, 1 M) and absorbance at 410 nm was recorded using Synergy H1 hybrid multi-mode reader (BioTek). The value of each group measured at day 0 was set as 1.
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bioRxiv - Physiology 2023Quote: ... the exact number of nematode in each well was determined on a Cell Imaging Multi-Mode Reader (Cytation 1, BioTek) and was used for normalising/correcting OCR results ...
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bioRxiv - Microbiology 2023Quote: ... The absorbance at λ420nm was measured every minute for 1-2 h to follow the kinetics of ONPG hydrolysis at 28 °C in a Synergy H1 plate reader (BioTek). The velocity of the reaction was calculated by performing linear regression using GraphPad Prism software ...
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bioRxiv - Microbiology 2023Quote: ... supernatant or size exclusion purified vesicles were extracted with butanol (1:2) overnight at 4°C and the butanol fraction transferred to microtiter plates for absorbance measurement (Synergy, Biotek).
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bioRxiv - Physiology 2023Quote: ... the exact number of nematode in each well was determined on a Cell Imaging Multi-Mode Reader (Cytation 1, BioTek) and was used for normalising/correcting OCR results ...
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bioRxiv - Immunology 2024Quote: ... samples were qualitatively assessed using the Take3 plate on the Cytation 1 cell imaging multi-mode reader with Gen5 software (BioTek). Samples which met established quality guidelines set by the sequencing facility were sent to University of Texas Genomic Sequencing and Analysis Facility for TagSeq library prep and sequencing on NovaSeq with single end ...
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bioRxiv - Immunology 2021Quote: ... Fluorescence was measured (ex = 370 nm/em = 460 nm) for 1 hour with a Cytation 3 imaging reader (BioTek, Winooski, VT). Changes in fluorescence were converted to thrombin concentrations using a calibration curve created from sequential dilutions of human thrombin ...
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bioRxiv - Synthetic Biology 2020Quote: ... and incubated at 37 °C (with a 1 °C gradient to minimize condensation) while shaking at 807 double-orbital cycles per minute in a plate reader (BioTek, Neo2SM). Optical density at 600 nm (OD600 ...
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bioRxiv - Biochemistry 2021Quote: ... The released pNP (extinction coefficient ε=16,000 M-1cm-1) was measured at 405 nm using Epoch 2 plate reader (Biotek, US) set to 37°C and subtracting the blank reading ...
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bioRxiv - Plant Biology 2021Quote: ... 100 μL of supernatant were added to 100 μL of 1 N NaOH and the absorbance of the solution was measured at 440 nm using an Epoch microplate spectrophotometer (BioTek, USA).
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bioRxiv - Cell Biology 2020Quote: ... was measured at 37°C from 1 to 10 min using a fluorescence microtiter plate reader (Synergy HTX Multi-Mode Reader, BioTek, USA). eNOS activity was expressed as the VEGFA-dependent increase in fluorescence per μg of cellular protein ...
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bioRxiv - Bioengineering 2022Quote: Luminescence was measured on a plate reader (BioTek Synergy™ HTX for collecting data presented in Figures 1, 3, 5, S5, S8, S12, S14; BioTek Synergy™ Neo2 for Figures 2C-D ...
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bioRxiv - Biochemistry 2021Quote: ... Fluorescence was measured (ex = 370 nm/em = 460 nm) for 1 hour with a Cytation 3 imaging reader (BioTek, Winooski, VT). Changes in fluorescence were converted to thrombin concentrations using a calibration curve created from sequential dilutions of human thrombin ...
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bioRxiv - Microbiology 2020Quote: ... The reaction was followed by measuring the absorbance at 405 nm at 1-minute intervals for 10 minutes using a Microplate reader (BioTek, USA).
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bioRxiv - Molecular Biology 2021Quote: ... the samples’ absorbance was measured at 494 nm using a 1 cm pathlength cuvette and a plate reader spectrophotometer (PowerWave XS, BioTek Instruments). No free fluorophore was detected in the cassettes that had only AF488 (without collagen) ...
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bioRxiv - Microbiology 2022Quote: ... intracellular NR dye was extracted using a destaining solution (50% ethanol, 1% acetic acid and water) and absorbance was measured at 540 nm using a spectrophotometer (Biotek, Elx800). Percentage of inhibition of each drug was calculated normalizing data against the negative control absorbance (100% value).
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bioRxiv - Bioengineering 2022Quote: ... or four 75% washes (for 96-well plate) with starvation media (DMEM +1% P/S) using an automated plate washer (BioTEK ELx405). Experiments were performed 3-4 hours post-starvation ...
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bioRxiv - Physiology 2024Quote: ... live cells stained with Hoechst 33342 (1:2,000 dilution) (ThermoScientific, Cat#62249) were enumerated using Cytation 5 plate reader (BioTek Instruments - Agilent). Values obtained from the Mito-Stress test were normalized per 1,000 cells.
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bioRxiv - Cancer Biology 2024Quote: Serum insulin and insulin like growth factor 1 (IGF1) levels were measured by ELISA using a microplate reader (BioTek Instruments Inc.) and respective kits (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... Images of 2D-cultured RFP+ melanoma cells were captured using a Cytation 1 Cell Imaging Multi-Mode Reader (BioTek Instruments, Winooski, VT).
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bioRxiv - Biochemistry 2019Quote: ... Chemiluminescence was measured every 1-2 minutes over 1.5 hours at 37 °C using the Synergy H1 multi-detection microplate reader (Biotek, Bad Friedrichshall, Germany) in triplicates and chemiluminescence activity was expressed as integrated total counts as calculated by the addition of rectangles with unit width under individual data points ...
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bioRxiv - Bioengineering 2020Quote: ... luminescence was read immediately using a plate reader with a 1 second integration time (BioTek Synergy HT Plate Reader and Gen5 Software, BioTek Instruments, Inc.). Relative luminescence for each sample was calculated by subtracting the luminescence from the sample blank (cell medium ...
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bioRxiv - Microbiology 2020Quote: ... Samples were examined using an Olympus 1×81 spinning disk confocal microscope (Tokyo, Japan) or Cytation 5 Cell Imaging Multi-Mode Reader instrument (Biotek, Winooski, VT). Images were analyzed using Volocity or Gen5 software ...
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bioRxiv - Microbiology 2019Quote: ... Maximum specific growth rates (μmax) presented in Table 1 were assayed at 30 °C using a Synergy HTX multi-mode microplate reader (BioTek, Winooski, VT) using 48-well plates ...
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bioRxiv - Bioengineering 2023Quote: ... It was then incubated for 1 hour at room temperature before the absorbance was read at 570 nm using a microplate reader (BioTek, Cytation 3). The manufacturer’s standard protocol was used to calibrate the measurements.
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bioRxiv - Microbiology 2023Quote: ... was recorded every 1 min over a period of 4 hours at 37°C using the Cytation 5 microplate reader (BioTek Instruments, USA).
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bioRxiv - Synthetic Biology 2023Quote: ... The absorbance at 405 nm was measured every 1 min for at least 30 min and up to 2 h using a Synergy 4 microplate reader (Biotek, Winooski, VT). SEAP activities were calculated as a function of the increase in absorbance at 405 nm over time42.
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bioRxiv - Physiology 2023Quote: ... was incubated with a working calcium color reagent for 1 min and the absorbance read at 575 nm using a spectrophotometer (BioTek Synergy HTX).
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bioRxiv - Biochemistry 2023Quote: ... Reactions were started by addition of 1 mM Mg-ATP and NADH absorbance was monitored at 340 nm in a plate reader (Biotek PowerWave HT) at 26°C ...
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bioRxiv - Microbiology 2024Quote: ... The remaining crystal violet was fully dissolved in 1 ml 95% ethanol with constantly shaking and measured its optical density at 590 nm (Biotek microplate reader). The assay was repeated at least three times with similar results.
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bioRxiv - Microbiology 2024Quote: ... The growth behavior of the different mutants of AB5075-VUB was assessed in liquid culture using the Cytation 1 plate reader (BioTek, United States). Each well of a 96-well plate was filled with 200 µL of bacterial culture diluted to an initial OD600 of 0.1 ...
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bioRxiv - Immunology 2021Quote: ... and absorbance was measured at 450 nm using a plate reader after stopping the reaction with 50 μl of 1 N HCl (Biotek Epok, Model # 3296573).
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bioRxiv - Immunology 2021Quote: ... and absorbance was measured at 450 nm using a plate reader after stopping the reaction with 50 μl of 1 N HCl (Biotek Epok, Model # 3296573).
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bioRxiv - Biochemistry 2020Quote: ... 30 μL of a 10 nM solution of FphF in PBS/0.01%TritonX-100 was added and fluorescence (λex = 365 nm and λem = 455 nm) was read at 37°C in 1 min intervals on a Cytation 3 imaging reader (BioTek, Winooski, VT, USA) for 60 min ...
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bioRxiv - Cancer Biology 2021Quote: ... and the percentage of GFP+ CAFs or RFP+ melanoma cells was determined using a Cytation 1 Cell Imaging Multi-Mode Reader (BioTek Instruments, Winooski, VT). The efficiency of β-catenin ablation by shRNA in CAFs was determined by Western blotting.
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bioRxiv - Genetics 2020Quote: ... 10 μl of ligand was added to each well and luminescence measurements were recorded immediately for 30 min at 1-min intervals (Synergy H1 Hybrid Multi-Mode Reader, BioTek, Winooski, VT, USA).
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bioRxiv - Cancer Biology 2022Quote: ... Co-culture was imaged at 1-hour intervals for up to 72 hours using an Agilent® Biotek® LionHeart™ FX (BioTek) inverted Motorized Widefield Fluorescence Microscope ...
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bioRxiv - Bioengineering 2022Quote: ... Absorbency was measured immediately after adding stop solution at 450 nm on a plate reader (Citation 1 imaging reader, BioTek, Winooski, VT, US).
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bioRxiv - Plant Biology 2022Quote: ... The DNA resulting from this extraction was assessed for quality in a 1% agarose gel and quantified using Synergy™ HT Multi-detection microplate reader (BioTek®, USA). RAD-tag libraries were developed by the Beijing Genomic Institute (BGI ...
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bioRxiv - Microbiology 2023Quote: ... Fluorescence was measured at 50°C over 1 h at excitation 360 nm emission 460 nm on a BioTek Synergy HTX Multimode Reader (BioTek, Agilent, Tx, USA). The reaction rate was calculated as the maximum velocity over 12 sequential readings ...
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bioRxiv - Bioengineering 2024Quote: ... and relative luminescence units at 1 s integration time were measured using a plate reader (Synergy HTX multimode plate reader, BioTek Inc., Winooski, VT). Relative ATP levels were calculated by normalizing the relative luminescence units (RLU ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then diluted 1:100 and incubated with increasing concentrations of antibiotics in the Synergy HT BioTek plate reader (BioTek Instruments Inc., USA) at 37°C for 16 hours ...
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bioRxiv - Microbiology 2020Quote: ... transferred to 24-well culture plate and the OD600 determined kinetically (1 point/h) using a BioTek Synergy 2 spectrofluorometer (BioTeK U.S., Winooski, VT, USA) at 25°C with continuous slow agitation ...
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bioRxiv - Cell Biology 2022Quote: ... lungs were lavaged with 1 ml of sterile PBS to evaluate lung vasculature permeability via microplate reader (495nM ex/519nm em) (Syngery Mx, BioTek Instruments, Inc., Winooski, VT) and tissues taken for histological analysis ...
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bioRxiv - Molecular Biology 2019Quote: ... The plate was incubated in the dark at room temperature for 45 minutes and subsequently read at 1 sec integrated reads in a luminescent plate reader (Biotek Synergy 4 plate reader).
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bioRxiv - Bioengineering 2022Quote: ... The plates were incubated for 15 min at RT on an orbital shaker in the dark and relative luminescence units were measured at 1 s integration time using a Synergy HTX multimode plate reader (BioTek Instruments Inc., Winooski, VT).
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bioRxiv - Immunology 2023Quote: ... Colorimetric immune assays were run in triplicate on 96 well plates using a Cytation 1 cell imaging multi-mode reader and Gen 5 software (BioTek Instruments, Winooski, VT, USA). A Red660 (G Biosciences ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μL of substrates was added and fluorescence (λex◻=◻365◻nm and λem◻=◻455◻nm) was read at 37°C in 1 min intervals on a Cytation 3 imaging reader (BioTek, Winooski, VT, USA) for 60◻min ...