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Citations for Biotek :
101 - 150 of 880 citations for 6 Isoquinolinol 1 2 3 4 tetrahydro 1 4 methylphenyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 10 µL detection reagent was mixed with 10 µL sample in a white-walled 384 well plate and incubated for 1 hour at room temperature before data acquisition using a Synergy 2 plate reader (BioTek). Individual readings for NADH and NAD+ were compared to generate NADH:NAD+ ratios ...
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bioRxiv - Microbiology 2023Quote: ... The suspension was diluted (1:5) and the optical density at 600 nm (OD600) was measured with the Epoch 2 microplate reader (BioTek). The Relative Luminescent Units (RLU ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Fluorescence intensity was measured every 42 seconds for a duration of 1 hour at 37°C with a plate reader (Synergy 2, Biotek) (Temperature 37°C ...
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bioRxiv - Microbiology 2023Quote: ... The absorbance at λ420nm was measured every minute for 1-2 h to follow the kinetics of ONPG hydrolysis at 28 °C in a Synergy H1 plate reader (BioTek). The velocity of the reaction was calculated by performing linear regression using GraphPad Prism software ...
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bioRxiv - Microbiology 2020Quote: ... was added to each well to stop the reaction and the optical density (OD) was measured at 492 nm on a Synergy 4 plate reader (BioTek). An average of OD values for blank wells plus three standard deviations was used to set a cutoff for plate blank outliers ...
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bioRxiv - Microbiology 2019Quote: ... The resazurin was diluted at 0.004% in regular D10 medium and incubated with host cells at 37°C with 5% CO2 for 4 h before absorbance measurement at 570 and 600 nm by BioTek H1 Hybrid plate reader ...
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bioRxiv - Microbiology 2019Quote: ... The plates were sealed with a semi-permeable film (Thermo Fischer) and incubated at 30°C in a Synergy 4 plate reader (BioTek) with shaking and absorbance readings (600 nm ...
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bioRxiv - Biochemistry 2019Quote: ... Enzyme velocity was determined by measuring the formation of the 4-nitrophenolate product of hydrolysis (405 nm) for four minutes at room temperature using an Epoch microplate spectrophotometer (BioTek). Initial rates were corrected for non-enzymatic hydrolysis and the Michaelis constant was determined by fitting the initial rates to the Michaelis-Menten equation using GraphPad Prism (Supplementary Fig ...
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bioRxiv - Microbiology 2020Quote: ... and then optical density and fluorescence were monitored for 48 hours in an Biotek Synergy 4 plate reader (BioTek, USA) at 30 °C with fast continuous shaking ...
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bioRxiv - Cell Biology 2022Quote: ... Intracellular Fluo-4 AM was excited using automatic high-throughput living cell imaging and analysis system (BioTek, Lionheart FX, USA) at 494 nm ...
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bioRxiv - Synthetic Biology 2019Quote: ... and then optical density and fluorescence were monitored for 48 hours in a Biotek Synergy 4 plate reader (BioTek, USA) at 30 °C with fast continuous shaking ...
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bioRxiv - Synthetic Biology 2019Quote: ... and then optical density and fluorescence were monitored for 48□hours in an Biotek Synergy 4 plate reader (BioTek, USA) at 30□°C with fast continuous shaking ...
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bioRxiv - Biochemistry 2019Quote: ... Decrease in Cy5 fluorescence was monitored by measuring fluorescence at 680 nm by excitation at 635nm on a Synergy 4 plate reader (BioTek).
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bioRxiv - Biochemistry 2019Quote: Fluorescence anisotropy experiments were carried out at room temperature with 5 nM fluorescein (6FAM)-labelled DNA substrates (Table S2) using a Synergy 4 plate reader (BioTek). Titration with protein was performed by serially diluting protein in 40 μL of assay buffer (20 mM Tris Acetate pH 7.0 ...
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bioRxiv - Microbiology 2020Quote: ... the reaction was stopped with 50 μls of 3M hydrochloric acid and absorbance was measured at 490 nm (OD490) using a Synergy 4 (BioTek) plate reader ...
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bioRxiv - Microbiology 2022Quote: ... Fluorescence of released 4-methylumbelliferone (4MU) was measured (Excitation 360 nm, Emission 450 nm) in a plate reader (Synergy MX, Biotek). One unit of chitinase activity releases 1 μmol of 4MU from the appropriate substrate per minute ...
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bioRxiv - Bioengineering 2022Quote: ... Pellet was then resuspended in 1 mL of M9 medium and flasks were inoculated to the starting OD600 of 0.1 and incubated at 30 °C with agitation (233 RPM for cultures with substrate concentration 4 g/L and 275 RPM for cultures with substrate concentration 8 g/L) using NB205 orbital incubator (N-Biotek). The ratio of medium to flask volume was 1:5 (20 mL:100 mL ...
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bioRxiv - Microbiology 2022Quote: ... Plates were incubated at RT for 10 minutes in the dark before luminescence was measured using a Synergy 4 (BioTek) plate reader ...
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bioRxiv - Neuroscience 2023Quote: ... Absorbance at 490 nm was measured 4 hours after the addition of the MTS dye using a SynergyNEO2 plate reader (BioTek).
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bioRxiv - Physiology 2023Quote: ... The cells are first washed from their culture medium by 4 washes of KRB-BSA with the EL406 washer distributor liquid handling device (Biotek) suitable for 384-well plates ...
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bioRxiv - Microbiology 2023Quote: ... The reading process for multiple plates is automated using a BioStack™ 4 microplate stacker (BioTek Instruments, Inc., Winooski, VT), which cycles the experimental 96-well microplates and removes the lids at the specified interval as to not interfere with spectrophotometric values ...
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bioRxiv - Microbiology 2022Quote: All bacteria were incubated anaerobically in the 24-well plate at 37°C for 3 hours in an Epoch 2 Microplate Spectrophotometer (BioTek), with optical density measured at 600 nm (OD600) ...
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bioRxiv - Neuroscience 2023Quote: ... Fluorescence emission (528, 590 nm) was measured over the course of 3 hours using a Synergy 2 microplate reader (Biotek) with excitation at 485 nm.
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bioRxiv - Biochemistry 2021Quote: ... The released pNP (extinction coefficient ε=16,000 M-1cm-1) was measured at 405 nm using Epoch 2 plate reader (Biotek, US) set to 37°C and subtracting the blank reading ...
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bioRxiv - Microbiology 2019Quote: ... clear bottom 96-well plate and absorbance was measured at 425 nm in a Biotek Synergy 4 plate reader (BioTek, USA). A standard curve was made with bisdemethoxycurcumin standards dissolved in oleyl alcohol (Figure S3)
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bioRxiv - Molecular Biology 2021Quote: ... The kinetics of substrate cleavage (increase in fluorescence of the liberated 7-amino-4-methylcoumarin, AMC) was measured using a fluorometer (BioTek, USA) set at 370 nm excitation and 460 nm emission at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Endpoint titers were determined by 4-parameter analysis using the Gen5™ 2.0 Data Analysis software (BioTek Instruments, Inc., Winooski, VT) and defined as the reciprocal of the serum dilution producing a signal identical to that of negative control samples at the same dilution plus three times the standard deviation.
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bioRxiv - Biochemistry 2019Quote: ... Chemiluminescence was measured every 1-2 minutes over 1.5 hours at 37 °C using the Synergy H1 multi-detection microplate reader (Biotek, Bad Friedrichshall, Germany) in triplicates and chemiluminescence activity was expressed as integrated total counts as calculated by the addition of rectangles with unit width under individual data points ...
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bioRxiv - Synthetic Biology 2022Quote: ... with Cytation 1 (BioTek), or visualized with a blue light or UV transilluminator ...
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bioRxiv - Neuroscience 2021Quote: ... Uptake of MEs labeled with FM 4-64 and RNA cargos labeled with ExoGlow-RNATM was quantified by using a microplate fluorescence reader (BioTek Instruments, Inc.) and confocal microscopy imaging respectively ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 11.1 μl of Alamar blue reagent was added and the readings were taken after 4 hours in a Synergy H1 plate reader (BioTek, Winooski, VT) with 560 nm (excitation ...
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bioRxiv - Microbiology 2021Quote: ... 4 and 5 were performed using two plate readers: A Synergy Neo2 and a Synergy H1 (both from Biotek Inc., VT, USA). Both were set to 30°C with continuous shaking at an orbital displacement of 1 mm and a speed of 807 rpm ...
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bioRxiv - Cell Biology 2022Quote: ... and changes in intracellular Ca2+ concentrations were measured by recording the ratio of fluorescence intensities at 508/20 nm (15 second time interval) using a Synergy 4 multimode microplate reader (BioTek, Winooski, VT). Traces were normalized to their respective baseline intracellular Ca2+ level obtained by averaging the 340/11 nm/380/20 nm ratios of each experiment in the absence of stimulus ...
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bioRxiv - Microbiology 2022Quote: ... The plate was incubated with shaking at 37 °C and the optical density of each well was measured at a wavelength of 600 nm (BIOTEK, Synergy 4). The MIC values were taken as the lowest concentration for which no growth was discernible (<0.05 OD600 ...
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bioRxiv - Microbiology 2023Quote: ... The resulting 1,472 SICs were arranged in 16 96-well plates and incubated in the anaerobic chamber at 37 °C (by setting the temperature of the chamber to 37 °C) in a plate stacker (BioTek BioStack 4) with 2 control plates containing uninoculated media at the top and bottom of the stack to minimize edge effects ...
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bioRxiv - Neuroscience 2023Quote: ... After 40 hours, cells were fixed in 4% PFA and images (eGFP, mCherry and eBFP2) were acquired at cytationC10 (Biotek, Objective 60x). eGFP (interaction signal ...
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bioRxiv - Neuroscience 2023Quote: ... and fluorescence emitted by Atto488 (λex = 485 nm, λem = 538 nm) was measured in a Synergy 4 plate reader (BioTek Instruments GmbH) at intervals of 10 seconds ...
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bioRxiv - Microbiology 2024Quote: ... NBT was reduced to blueformazan and spectrophotometrically read at OD 650 nm using a Synergy 4 microplate reader (BioTek, Santa Clara, USA). The concentration at which the drugs were able to inhibit 50% parasite growth (IC50 ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μL of substrates was added and fluorescence (λex◻=◻365◻nm and λem◻=◻455◻nm) was read at 37°C in 1 min intervals on a Cytation 3 imaging reader (BioTek, Winooski, VT, USA) for 60◻min ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2 h later the fluorescence was read at 340/505 and 380/505 on a Cytation™ 3 (BioTek (Vermont, MA, USA)) multifunctional microplate reader ...
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bioRxiv - Cell Biology 2022Quote: ... We also performed immunofluorescence staining for pluripotency markers in these iPSC colonies using Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit (Thermo Fischer Scientific) as per manufacturer’s instruction and fluorescence were imaged using fluorescence microscopy (BioTek Lionheart LX Automated Microscope) as described previously.15 Alkaline phosphatase (ALP ...
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bioRxiv - Cell Biology 2021Quote: ... Absorbances were obtained at 620 nm and sample concentrations were determined by comparison to the CXCL12 standards using a 4-parameter curve fit (Synergy HT & Gen5 Software, BioTek Instruments, Winooski, VT). Serum level of CXCL12 was assessed using undiluted serum isolated from the blood of tumor-bearing mice or stage IV glioma patients.
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bioRxiv - Microbiology 2020Quote: ... on Cytation 3 (BioTek) multimode plate imager according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... on Cytation 3 (BioTek). Virus concentrations that gave signal higher than 105 RLUs/well were used in neutralization experiments ...
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bioRxiv - Microbiology 2020Quote: ... transferred to 24-well culture plate and the OD600 determined kinetically (1 point/h) using a BioTek Synergy 2 spectrofluorometer (BioTeK U.S., Winooski, VT, USA) at 25°C with continuous slow agitation ...
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bioRxiv - Microbiology 2021Quote: ... Three replicates of 150 µL of each sample was added to a 96-well plate and incubated with moderate shaking at 37°C (BioTek Synergy 4 Plate Reader) and the OD600 was measured at 30 min intervals for 16 h ...
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bioRxiv - Microbiology 2023Quote: ... We performed PCA oxidation assays either by tracking the fluorescence of reduced PCA over time in a plate reader (BioTek Synergy 4 or HTX) housed in a Coy-brand anoxic chamber or by tracking the current generated by a culture when provided PCA and an electrode poised to continuously reduce any PCA that the cells oxidized ...
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bioRxiv - Immunology 2022Quote: ... Reactions were stopped by adding 50 μL 3M hydrochloric acid and absorbance at 492 nm was determined on a Synergy 4 plate reader (BioTek, Agilent Technologies inc., CA, USA) or similar ...
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bioRxiv - Microbiology 2020Quote: ... UC500) and growth at 37 °C was monitored by measuring optical density at 600 nm every 15 minutes (Biotek Eon instrument with a BioStack 4). Carbon sources tested include D-glucose ...
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bioRxiv - Biochemistry 2023Quote: The polymerization and depolymerization of pyrene–labeled recombinant WT or mutant actin (5% pyrene-labeled WT or mutant actin) was monitored as a function of increasing or decreasing pyrene– fluorescence using a Synergy 4™ microplate reader (BioTek Instruments, Winooski, VT, USA) and the built–in filter set (Excitation ...