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Citations for Biotek :
351 - 400 of 675 citations for 6 Diazo 5 6 dihydro 5 oxo 1 naphthalenesulfonic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Total RNA quantity and purity were assessed using a Cytation 5 Cell Imaging Multi-Mode Reader (Biotek, Winooski, United States) with the Take3 microvolume plate accessory (absorbance read at 230 nm ...
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bioRxiv - Microbiology 2021Quote: ... 75,000 effector cells/well were added to the plate and incubated for 6 h at 37°C.The luciferase substrate was added in the dark and the luminescence activity measured after 5 minutes using a Synergy Hybrid Reader (BioTek). The data was analysed using GraphPad prism 7.
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bioRxiv - Microbiology 2019Quote: ... The resazurin was diluted at 0.004% in regular D10 medium and incubated with host cells at 37°C with 5% CO2 for 4 h before absorbance measurement at 570 and 600 nm by BioTek H1 Hybrid plate reader ...
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bioRxiv - Evolutionary Biology 2021Quote: ... was added to the cells and incubated in the dark for 5 min before reading on a Synergy H1 Hybrid Multi-Mode plate reader (Biotek). Normalized cell entry levels of pseudovirus generated on different days (biological replicates ...
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bioRxiv - Immunology 2020Quote: ... Images were acquired in random fields at 20x magnification in brightfield and GFP (469nm/525nm) channels using Cytation 5 Imaging Multi-Mode Reader (Biotek) at 3 minutes intervals for 6 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were allowed to lyse for 5 minutes and then luminescence measured with a microtiter plate reader (Biotek, Winooski, VT).
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bioRxiv - Plant Biology 2019Quote: ... The image acquisition and analysis of roots and shots were performed in Cytation™ 5 cell imaging multimode reader and Gen3.03 software (BioTek). Venus was excited at 488 nm and detected at 498-530 nm ...
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bioRxiv - Immunology 2021Quote: ... Assay plates were placed on an orbital sharker for 2 min and incubated at 25 °C for 10 min before luminescence was read on a Cytation 5 multimodal plate reader (BioTek). The data was exported to spreadsheet software for analyses and visualized with GraphPad PRISM software (v 9.2.0).
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bioRxiv - Immunology 2021Quote: ... Fluorescence intensity was then measured for resazurin (535 nm/590 nm) and CFDA-AM (484 nm/530 nm) using a Cytation 5 multi-mode imaging plate reader (BioTek). In all cases ...
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bioRxiv - Cancer Biology 2021Quote: The proliferation assay was performed by DAPI staining of the cell nuclei and automatically counted by a CytationTM 5 Cell Imaging Reader (BioTek). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were centrifuged at 12000 rpm for 5 minutes and the absorbance of supernatants was read at 475nm in the Synergy-H1 microplate reader (BioTek). Absorbance values were compared to the standard curve of synthetic melanin (155343 ...
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bioRxiv - Neuroscience 2020Quote: ... Fluorescence intensity of each well containing AβpH was obtained on a Cytation™ 5 imaging multi-mode reader (BioTek Instruments) at 443/505 nm excitation/emission wavelengths ...
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bioRxiv - Neuroscience 2020Quote: ... The concentration of Aβ1-42 was determined by the absorbance value detected using a microplate reader (BioTek Cytation 5, US) at 450 nm and 590 nm.
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bioRxiv - Cell Biology 2021Quote: ... Bright field images of colonoids were collected at three different focal planes over the Matrigel drop using a Cytation 5 imager (BioTek). Colonoids that were in focus were scored as either spheroid or non-spheroid ...
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bioRxiv - Microbiology 2021Quote: ... The fluorescence signals were read at a 365 nm excitation wavelength and a 450 nm emission wavelength using a Cytation 5 Cell Imaging Multi-Mode Reader (Biotek) and a standard curve was created ...
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bioRxiv - Microbiology 2020Quote: ... erythromycin was added to the culture to a final concentration of 15 µg/ml (5-fold MIC) and growth curves were acquired for 20 h using a microplate spectrophotometer (EON, Biotek) by measuring the OD578 every hour after 30 sec of linear shaking.
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bioRxiv - Cancer Biology 2020Quote: ... were added to each well and after 2 hours of incubation at 37°C and 5% CO2 450 nm absorbance was read on a Synergy 2 plate reader (BioTek).
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bioRxiv - Cell Biology 2020Quote: Cells were imaged in either a 96- or 24-well dish every two or four hours for up to five days using the Cytation 5 Cell Imaging Multi-Mode Reader (Biotek) driven by Gen5 software (Biotek) ...
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bioRxiv - Microbiology 2022Quote: ... The plates were further incubated for six hours at 37°C in a 5% CO2 atmosphere and then analyzed spectrophotometrically (Synergy H1, Biotek) at 640 nm ...
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bioRxiv - Biochemistry 2022Quote: ... by measuring the absorbance at 600 nm over time of samples in a 96-well clear plate (Costar) using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek). To remove background absorbance ...
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bioRxiv - Physiology 2022Quote: ... Images of each well were captured every 24 hours for the duration of the experiment using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek).
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bioRxiv - Immunology 2022Quote: ... We measured the cGAMP concentration in the filtrates against a calibration curve of cGAMP at 260 nm using Take3 Micro-Volume absorbance analyzer of Cytation 5 (BioTek). We calculated the final concentration of cGAMP in NanoSTING and encapsulation efficiency by subtracting the concentration of free drug in the filtrate.
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bioRxiv - Cancer Biology 2021Quote: ... for 10 min at room temperature and the imaging acquisition was performed by using DAPI 355nm and RFP 558nm on Cytation 5 Cell Imaging Multi-Mode Reader (BioTek). The percentage of positive cells was calculated with a mask function created on the Microscope Software.
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bioRxiv - Cancer Biology 2021Quote: ... The methanol was removed and the CSK buffer was added before imaging nucleus areaacquisition by using DAPI 355nm on Cytation 5 Cell Imaging Multi-Mode Reader (BioTek). The Nucleus area of cells was acquired by a mask function created on the Microscope Software.
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bioRxiv - Cancer Biology 2021Quote: ... Cell imaging acquisition was performed by using GFP 488nm and DAPI 355nm on Cytation 5 Cell Imaging MultiMode Reader (BioTek).
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bioRxiv - Biophysics 2020Quote: ... Fluorescence images of approximately ten thousand cells were acquired per well with a 10x objective in a Cytation 5 (BioTek). The total number of cells ...
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bioRxiv - Microbiology 2020Quote: ... 5 minutes) to remove the beads and the absorbance at 570 nm was measured from the resulting supernatant (Biotek Eon). One million amine plus phosphonate beads were analyzed ...
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bioRxiv - Microbiology 2022Quote: ... 75 µL of culture media was added for each well and the expression of EGFP was monitored every 20 min for 28 hours at 37 °C in Cytation 5 (BioTek) provided with 5% CO2 ...
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bioRxiv - Systems Biology 2022Quote: ... the wells were imaged using the TagBFP (Excitation-390nm, Emission-447nm) and RFP filters (Excitation-531/40 nm, Emission-593/40 nm) in Cytation 5 (Biotek). Each image was flatfield corrected and background subtracted using CellProfiler ...
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bioRxiv - Biochemistry 2019Quote: ... Developing reagent and quench reagent were delivered sequentially by an EL406 automated multi-channel liquid dispenser with 5 µL Dispense Cassette (BioTek). Absorbance was determined at λ= 620 nm (A620 ...
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bioRxiv - Bioengineering 2020Quote: ... The plate was incubated in the dark at room temperature for 5 minutes followed by fluorescent quantification using an excitation/emission of 485/520 nm (BioTek Cytation5 spectrofluorometric plate reader) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Fluorescence measurement was performed one hour after incubation at 37 °C and 5 % CO2 for each cell line with the Synergy HT microtiter plate reader (BioTek Instruments Inc. ...
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bioRxiv - Microbiology 2019Quote: ... The OD600 nm of the cultures was measured after 24 h incubation at 37°C in a 5% CO2 aerobic atmosphere with a Synergy 2 multimode microplate reader (BioTek). The experiment was performed at least four times.
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bioRxiv - Systems Biology 2019Quote: ... Fluorescence intensity with an excitation wavelength of 380 nm and an emission wavelength of 460 nm was recorded at 5-minute intervals using a Neo2 plate reader (BioTek) set to mix constantly and maintain 30 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... LDL uptake was determined by quantification of the fluorescence signal using a multi-mode fluorescent microplate reader (Cytation 5, Biotek), and corrected for the protein contents in the same lysates as determined with the BCA assay ...
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bioRxiv - Microbiology 2019Quote: ... The mixture was incubated at room temperature for 5 min and the absorbance (A650) was read using a Synergy H4 microplate reader (BioTek). Inhibition was observed with varying concentrations of 1.
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bioRxiv - Biochemistry 2019Quote: Fluorescence anisotropy experiments were carried out at room temperature with 5 nM fluorescein (6FAM)-labelled DNA substrates (Table S2) using a Synergy 4 plate reader (BioTek). Titration with protein was performed by serially diluting protein in 40 μL of assay buffer (20 mM Tris Acetate pH 7.0 ...
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bioRxiv - Microbiology 2019Quote: ... or RFP (excitation 555 nm, emission 610 nm) relative fluorescence units (RFU) recorded every 5 min with the Gen5 (BioTek) software ...
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bioRxiv - Developmental Biology 2019Quote: ... Firefly and Renilla bioluminescence readings were obtained from 20 μl of cleared lysate using the Synergy 2 plate reader and Gen 5 analysis software system (BioTek). Firefly bioluminescence was normalized to renilla levels ...
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bioRxiv - Neuroscience 2020Quote: ... The intrinsic tryptophan fluorescence of L-PGDS was measured using Cytation 5 cell imaging multi-mode (Biotek Instrument Inc., USA) reader with λex=295 nm and λem=340 nm ...
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bioRxiv - Immunology 2020Quote: ... Fluorescence levels were recorded every 5 minutes over a period of 500 minutes using a Synergy H1 hybrid reader system (Biotek) at excitation/emission wavelengths of 488/515 nm ...
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bioRxiv - Neuroscience 2021Quote: ... Changes in medium pH during hypercapnia were measured by Phenol Red absorbance at 415 and 560 nm using the Cytation 5 Multi-Mode Reader (BioTek)89 ...
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bioRxiv - Immunology 2020Quote: ... Cells were imaged on a LSM 780 confocal microscope (Zeiss) with a 40 or 63x oil-immersed objective with Airyscan or a Cytation 5 (BioTek) with a 20x objective ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Fluorescence images of approximately ten thousand cells were acquired per well with a 10x objective in a Cytation 5 (BioTek). The total number of cells ...
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bioRxiv - Biochemistry 2021Quote: ... OD600 as well as GFP fluorescence (485 nm excitation, 528 nm emission) was measured in a microplate reader (Cytation 5 BioTek) and the fluorescence signal was normalized to OD600 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stained with Hoechst 33342 and counted for GFP+ and total cells with Cytation 5 high-content live-cell imaging system (Biotek) using a 4x objective.
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bioRxiv - Bioengineering 2020Quote: ... Well plates were always protected from light and fluorescence measurements were carried out at 37°C and 5% CO2 at Cytation 3 (Cell Imaging Multi-Mode Reader) from Biotek® (USA) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Baseline fluorescence intensity was measured with excitation at 350 nm and emission at 435 nm and 500 nm for 5 min at 37°C on a Synergy H1 spectrophotometer (BioTek). Cells were then treated with 10% ethanol as a membrane fluidizer ...
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bioRxiv - Microbiology 2021Quote: ... Equal volumes of the cell suspension and the BacTiter-Glo reagent were mixed and incubated for 5 min before measuring luminescence using a Synergy Mx Monochromator-Based Multi-Mode Microplate Reader (BioTek). Signal intensities were corrected for cell numbers by dividing the luminescence signal by the OD 595 nm of the cell suspension ...
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bioRxiv - Microbiology 2020Quote: ... The cultures were diluted back to a calculated OD 600 of 0.05 (replicated in 2-5 wells for each culture) and their growth measured using an Epoch microplate spectrophotometer (BioTek, VT) at 37 °C with continuous shaking ...