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Citations for Biotek :
1 - 50 of 612 citations for 2S 4S 4 Fmoc amino pyrrolidine 2 carboxylic acid methylester hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Plates were developed for 10 minutes at room temperature and then the reaction was stopped by adding 50 μL 3 M hydrochloric acid (HCl, Fisher) and plates were read using a Synergy 4 (BioTek) plate reader at an optical density (OD ...
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bioRxiv - Plant Biology 2020Quote: ... The total amino acid content was then measured in a Synergy HT plate reader (BioTek, Germany) at a wavelength of 550 nm ...
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bioRxiv - Microbiology 2021Quote: ... stopped using 3M hydrochloric acid (HCL) solution and plates were read at 490nm wavelength using ELISA plate reader (BioTek Instruments). The endpoint antibody titer was determined by using a cut-off value which is three-times the absorbance of first dilution of mock (uninfected ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and used to generate sequences with the desired amino acid substitution using Q5 Site-Directed Mutagenesis Kit (NEB, via BioTek, Praha, Czech Republic). Primers were designed using NEBaseChanger (https://nebasechanger.neb.com/) ...
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bioRxiv - Molecular Biology 2021Quote: ... The kinetics of substrate cleavage (increase in fluorescence of the liberated 7-amino-4-methylcoumarin, AMC) was measured using a fluorometer (BioTek, USA) set at 370 nm excitation and 460 nm emission at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... removal by vegetative cells was measured by sampling supernatant and assaying AcA using the change in absorbance at 380 nm in the presence of 2--amino benzamidoxime (ABAO), (as inspired by aldehyde quantifications by Ressmann et. al, 2019) using an Epoch microplate reader (BioTek; Gen5 v3.10 software) [42] ...
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bioRxiv - Microbiology 2020Quote: ... the reaction was stopped with 50 μls of 3M hydrochloric acid and absorbance was measured at 490 nm (OD490) using a Synergy 4 (BioTek) plate reader ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were quenched with 2 M sulfuric acid before determining absorbance at 450 nm using a Synergy BioHTX plate reader (BioTek). The background value from wells containing no serum was averaged and subtracted from all wells on the plate.
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bioRxiv - Cancer Biology 2024Quote: ... the staining was eluted with 10% acetic acid solution and the optical density was measured at 570 nm in the spectrophotometer Epoch 2 (Biotek).
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bioRxiv - Immunology 2021Quote: ... The reaction was stopped with 50 µL of 2N sulfuric acid and the absorbance at 450 nm was read with an Epoch 2 microplate reader (Biotek, USA).
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bioRxiv - Evolutionary Biology 2022Quote: ... Culture OD600 was recorded every 4 minutes over 24 hours in an Epoch 2 plate reader (BioTek) set to 300 rpm and 30°C.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The O.D./min was continuously recorded at 405 nm over 30 min to measure the hydrolytic product 5-mercapto-2-nitrobenzoic acid using a Synergy H1 microplate reader (Biotek; VT, USA). O.D./min was converted to catalytic rate using the Beer-Lambert law:
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bioRxiv - Immunology 2022Quote: ... Reactions were stopped by adding 50 μL 3M hydrochloric acid and absorbance at 492 nm was determined on a Synergy 4 plate reader (BioTek, Agilent Technologies inc., CA, USA) or similar ...
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bioRxiv - Immunology 2022Quote: ... Reactions were stopped by the addition of 2M sulfuric acid and read using a BioTek Synergy 2 or Synergy H1 plate reader set at 450nm (BioTek, Winooski, VT, USA). Receiver operator curve (ROC ...
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bioRxiv - Immunology 2022Quote: ... 1N HCl was used as a stop reagent and the absorbance was measured by BioTek® 800 ™ TS at 450 nm and 630 nm ...
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bioRxiv - Cell Biology 2021Quote: ... Changes in intracellular Ca2+ concentrations were measured by recording the ratio of fluorescence intensities at 508/20 nm resulting from excitation of Fura-2 AM at 340/11 nm or 380/20 nm (center/bandpass) using a Synergy 4 multimode microplate reader (BioTek). Ratios were acquired every 0.7 seconds for 15 seconds before injection and 2 minutes after injection ...
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bioRxiv - Microbiology 2023Quote: ... supernatant or size exclusion purified vesicles were extracted with butanol (1:2) overnight at 4°C and the butanol fraction transferred to microtiter plates for absorbance measurement (Synergy, Biotek).
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bioRxiv - Cell Biology 2020Quote: Total protein levels were calibrated using Bicinchoninic Acid (BCA) assay (ThermoFisher Pierce BCA Protein Assay kit, OD measured with BioTek synergy 2 multi-mode multiplate reader). Samples were ran on a 9% SDS polyacrylamide gel ...
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bioRxiv - Synthetic Biology 2023Quote: ... The absorbance at 405 nm was measured every 1 min for at least 30 min and up to 2 h using a Synergy 4 microplate reader (Biotek, Winooski, VT). SEAP activities were calculated as a function of the increase in absorbance at 405 nm over time42.
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bioRxiv - Immunology 2021Quote: ... Synergy 4 (BioTek), at an absorbance of 490 nanometers ...
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bioRxiv - Neuroscience 2024Quote: ... Synergy 4 (BioteK) plate reader was used to determine the concentration of LCN2 ...
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bioRxiv - Immunology 2020Quote: ... The developing reaction was quenched by addition of 100µl 1N HCl and absorbance was measured at 450nm using Gen5 software on a Synergy Neo2 Reader (BioTek).
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bioRxiv - Immunology 2022Quote: ... 100 μl 1N HCL as stop solution was added to each well and the absorbance was measured by BioTek® 800 ™ TS at 450 nm and 630 nm (as Reference filter).
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bioRxiv - Molecular Biology 2022Quote: ... production catalyzed by SARM1 was measured after adding the reaction reagents containing 50 μM PC6 and 100 μM NAD in 50 mM Tris-HCl (pH 7.5) on a Synergy H1 Hybird Reader (BioTek). The initial rate (RFU/min ...
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bioRxiv - Immunology 2023Quote: ... the reaction stopped with 1 M HCl and the plates analyzed using a Synergy H1 microplate reader (BioTek Instruments) at 450 nm ...
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bioRxiv - Microbiology 2021Quote: ... 200 μl of the HCl supernatant layer containing the extracted pyocyanin was measured for absorbance at 520 nm with 0.2 M HCl as blank in a multi-mode plate reader (BioTek, USA). The concentration of pyocyanin was calculated by the formula [19]:
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bioRxiv - Microbiology 2021Quote: ... the reaction was stopped by adding 50 μl/well of 3M HCl and the plates read at an absorbance of 490 nm using a Synergy H1 hybrid multimode microplate reader (BioTek). The IC50 values were calculated by using GraphPad Prism 7.
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bioRxiv - Cell Biology 2024Quote: ... The MTT crystal were solubilized overnight in solubilization solution (10% SDS in 0.01N HCl) and absorbance was measured and at 570 nm and 660 nm was measured using Cytation 5 plate reader (BioTek) 21.
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bioRxiv - Immunology 2022Quote: ... Reactions were quenched by addition of 1 N HCl and absorbance at 450 nm were analyzed using a plate reader (BioTek). ELISAs with gp120s and anti-idiotype monoclonal antibodies were performed as above except these proteins were immobilized directly onto high-binding 96-well assay plates (Costar ...
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bioRxiv - Microbiology 2024Quote: ... The assay was developed using 3,3’,5,5’-tetramethylbenzidine (TMB) (SeraCare) followed by 1N HCl and the absorbance measured at 450 nm (BioTek Synergy H1). The endpoint dilution was calculated as the final dilution > 2-fold above the average background OD450 ...
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bioRxiv - Molecular Biology 2024Quote: ... 2-4 images at the central callus and distal callus regions were taken using an automatic microscope (Agilent, BioTek Lionheart FX, Santa Clara, CA). Central callus regions were defined as proximal to the fracture site on either side of the bone ...
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bioRxiv - Bioengineering 2024Quote: The reaction was stopped with 1 M aqueous HCl solution and a colorimetric readout was performed at 450 nm in absorbance using a Synergy H1 microplate reader (BioTek Instruments). Absorbance versus dilution curves of each dataset were generated and fitted with a five-parameter non-linear logistic regression using GraphPad 10 ...
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bioRxiv - Bioengineering 2021Quote: ... 25 μL of 3 N-HCl was added to each well and then the absorbance at 450 nm was recorded using a plate reader (BioTek Synergy HTX). Concentrations were calculated based on a standard curve ...
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bioRxiv - Immunology 2021Quote: ... and absorbance was measured at 450 nm using a plate reader after stopping the reaction with 50 μl of 1 N HCl (Biotek Epok, Model # 3296573).
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bioRxiv - Immunology 2021Quote: ... and absorbance was measured at 450 nm using a plate reader after stopping the reaction with 50 μl of 1 N HCl (Biotek Epok, Model # 3296573).
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bioRxiv - Cell Biology 2021Quote: ... Synergy 2 (BioTek).
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bioRxiv - Microbiology 2022Quote: ... measuring luminescence with Synergy 4 (Biotek). 1% of Triton-X100 and 0.25% DMSO were used as the positive and negative control ...
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bioRxiv - Plant Biology 2020Quote: ... Nucleic acid concentration was measured using a microplate reader Synergy HT (Biotek, Germany), with the software Gen5™ (Biotek ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... with the Nucleic Acid Quantification workflow on a Synergy HT plate reader (BioTek) with Gen5 software.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The nucleic acid concentration was determined using a microplate reader (Synergy H1; BioTek), and the quality of the RNA was assessed using Agilent 2100 Bioanalyzer system (Agilent Technologies) ...
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bioRxiv - Bioengineering 2023Quote: ... Images were taken by acquiring 2 × 2 images on Cytation5 (BioTek, USA) and analyzed on the Cytation 5 Gen5 software Image Prime v3.10 (Biotek,USA ...
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bioRxiv - Systems Biology 2020Quote: ... every 2 min during 24 to 36 h in a spectrophotometer (Synergy 2, Biotek) with temperature control and shaking (fast continuous setting) ...
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bioRxiv - Biochemistry 2021Quote: ... and colorimeter-EPOCH|2 (BioTek Instruments ...
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bioRxiv - Synthetic Biology 2022Quote: ... Epoch 2 microplate reader (BioTek) was used for anaerobic real-timer OD600 measurements ...
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bioRxiv - Biochemistry 2020Quote: ... using a BioTek Synergy 4 (BioTek, Winooski, VT) with the excitation and emission wavelengths of 485 nm and 528 nm ...
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bioRxiv - Microbiology 2021Quote: ... Fluorescence was measured (BioTek Synergy 4 Plate Reader) at an excitation wavelength of 620 nm and an emission wavelength of 685 nm at the start time ...
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bioRxiv - Microbiology 2020Quote: ... and an automated microplate stacker (Biostack 4, Biotek) fitted inside a custom-made incubator (EMBL Mechanical Workshop) ...
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bioRxiv - Microbiology 2023Quote: ... coupled with a Biostack 4 microplate stacker (Biotek), both housed inside an incubator (EMBL workshop) ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... and yields were quantified the Nucleic Acid Quantification workflow on a Synergy HT plate reader (BioTek) with Gen5 software ...
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bioRxiv - Systems Biology 2020Quote: ... or an Epoch 2 (BioTek Instruments) plate reader using Gen5 v ...