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Citations for Biotek :
401 - 450 of 609 citations for Human Mitochondrial Open Reading Frame Of The 12S rRNA c MOTS c CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... Red fluorescent protein (RFP) readings (excitation 580 nm, emission 610 nm) were taken using a BioTek Synergy H1m plate reader (BioTek, Winooski, VT) after 18 hours of incubation at 37 °C with 200 rpm shaking.
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bioRxiv - Bioengineering 2021Quote: ... after which the gels were transferred to a black 96-well plate and fluorescence readings were taken (BioTek Synergy H1 microplate reader).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 11.1 μl of Alamar blue reagent was added and the readings were taken after 4 hours in a Synergy H1 plate reader (BioTek, Winooski, VT) with 560 nm (excitation ...
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bioRxiv - Neuroscience 2019Quote: ... The plate was equilibrated at room temperature for 30 min to stabilize luminescent signal before reading with a Synergy H1 Microplate Reader (BioTek, Winooski, VT).
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bioRxiv - Microbiology 2020Quote: ... pH 7 by reading absorbance at 486 nm every minute for 15 minutes at room temperature in microtiter plates (Biotek Synergy H1M). β-lactamase activity was calculated as initial reaction rate (Vmax ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... acetic acid solution to solubilize the dye incorporated within the cells followed by a spectrophotometric reading at a wavelength of 595 nm using a microplate reader (Synergy HT BioTek, Milan, Italy).
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bioRxiv - Biochemistry 2019Quote: ... Plates were agitated at 25°C for 10 min and absorbance was recorded at 590 nm using a multi-well plate reader (Biotek-Epoch; Biotech Instruments). The average absorbance value of five replicate wells was used for each set and each experiment was repeated thrice ...
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bioRxiv - Biochemistry 2020Quote: ... 30 μL of a 10 nM solution of FphF in PBS/0.01%TritonX-100 was added and fluorescence (λex = 365 nm and λem = 455 nm) was read at 37°C in 1 min intervals on a Cytation 3 imaging reader (BioTek, Winooski, VT, USA) for 60 min ...
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bioRxiv - Microbiology 2020Quote: At least three replicates per strain and growth condition were grown in 200 μl of LB alone or supplemented with canavanine in a 96-well plate at 30°C with 140 rpm shaking in a BioTek Eon Microplate Spectrophotometer (BioTek, Winooski, VT, USA). The A600 was measured at 10 minutes intervals ...
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bioRxiv - Microbiology 2020Quote: ... Growth was monitored by OD600 measurements for 96 hours at 28°C using a microplate spectrophotometer (Synergy HT Multi-Mode Reader, BioTek Instruments, Inc, USA). Culture plates were shaken every 25 minutes for 30 s prior to the OD600 measurements ...
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bioRxiv - Biochemistry 2021Quote: We measured the catalytic activity of the enzyme citrate synthase in mitochondrial samples monitoring the conversion of oxaloacetate and acetyl-CoA to citrate and CoA– SH and by measuring the formation of the colorimetric product thionitrobenzoic acid (TNB) at 412 nm and 37°C (Shepherd and Garland, 1969) on a microplate reader (Power Wave XS-2, Biotek Instruments, Winooski, USA). We calculated the enzyme activity using the changes in absorbance after substrate (250 µM oxaloacetate ...
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bioRxiv - Microbiology 2020Quote: ... Em = 450 nm) over a period of 30-60 minutes at 37°C on a Cytation-3 imaging reader (BioTek, Winooski, VT, USA). Concentrations of hydrolyzed products were determined using a standard concentration curve (unconjugated 4MU) ...
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bioRxiv - Microbiology 2022Quote: ... The cells were grown with agitation at 30°C for 30 hours in a microplate reader (Infinite M Plex; Tecan, Austria, or Synergy 2; BioTek, Winooski, VT, USA), and the optical density at 600 nm (OD600 ...
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bioRxiv - Systems Biology 2019Quote: ... Assay plates were incubated for 24 h (30°C, 160 rpm) in a Cytation3 cell imaging multimode reader (BioTek Instruments, Winooski, VT, USA). At 24 h ...
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bioRxiv - Synthetic Biology 2020Quote: ... Aerobic growth was monitored in technical duplicates or triplicates at 37°C in a BioTek Epoch 2 Microplate Spectrophotometer (BioTek, Bad Friedrichshall, Germany) by absorbance measurements (600nm ...
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bioRxiv - Microbiology 2019Quote: ... The plate was sealed with optically-clear tape and incubated at 30°C in the dark without shaking in a Synergy HT plate reader (Biotek, Winooski, VT, USA). Optical density at 405 nm (OD405 ...
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bioRxiv - Microbiology 2021Quote: ... These aerobic cultures were monitored periodically every 60 min for 37 °C growth at 600 nm in Synergy H1 plate reader (BioTek Instruments Inc, VT).
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bioRxiv - Cell Biology 2021Quote: ... was measured every 5 min for 2 h at 25°C using Synergy H1 Hybrid Multi-Mode Microplate Reader (BioTek, cat. no. H1MFDG). The rate of kinetic reaction (slope ...
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bioRxiv - Synthetic Biology 2021Quote: ... The OD600 was measured at 10 min intervals for 20 hr at 32°C in orbital shaking mode on a SynergyH1 plate reader (Biotek, Winooski, VT, USA). Growth data for each strain was obtained from three replicate wells and three independent cultures ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The OD600 was measured every 2 hours for 24 hours at 37°C and slow continuous shaking using a plate reader (Synergy HT BioTek, Winooski, VT, USA). We used population growth rate and yield as fitness measures (Chavhan et al. ...
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bioRxiv - Microbiology 2023Quote: ... Fluorescence was measured at 50°C over 1 h at excitation 360 nm emission 460 nm on a BioTek Synergy HTX Multimode Reader (BioTek, Agilent, Tx, USA). The reaction rate was calculated as the maximum velocity over 12 sequential readings ...
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bioRxiv - Microbiology 2023Quote: ... were statically incubated at 30 °C for 48h and biofilm measurements were done as previously reported (41) using a Cytation5 plate reader (BioTek, Winooski, VT, USA). The experiment was replicated three times in total with 36 technical replicates each ...
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bioRxiv - Biochemistry 2023Quote: ... Phosphorylation reaction progress was monitored by measuring fluorescence at excitation 530 nm and emission 590 nm every 2 minutes at 37 °C on a plate reader (BioTek Synergy Neo 2). The fluorescence units (RFU ...
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bioRxiv - Cell Biology 2023Quote: ... the stained cells were subjected to measurements of the absorbances of 455 nm and 525 nm at 37 °C using a plate reader equipped with kinetic mode (BioTek SynergyH4 Hybrid Reader).
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bioRxiv - Cancer Biology 2023Quote: ... was supplied by gas controller into the incubator of Cytation 5 multi-mode reader system equilibrated at 37 °C (BioTek; Winooski, VT, USA).
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bioRxiv - Microbiology 2023Quote: ... All endpoint assays and growth curves were carried out at 30°C and 548 rpm using a Synergy HTX plate reader (BioTek, Winooski, VT, USA) with OD600 readings measured every 30 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... was used for quantifying changes in mitochondrial membrane potential in live cells using a Synergy H1 Hybrid microplate reader (BioTek, Winooski, VT). The assay implements FCCP (carbonyl cyanide 4-(trifluoromethoxy ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were allowed to equilibrate for 10 min post-lysis followed by luminescence reading using a Cytation3 multimode plate reader (BioTek, Winooski, VT, USA) set to luminescence ...
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bioRxiv - Molecular Biology 2023Quote: ... and fluorescent readings (excitation = 485 nm, emission = 520 nm, gain = 70) were taken on a Synergy H1 Hybrid Multi-Mode Reader (BioTek, #11-120-535) using Gen5 Microplate Reader and Imager software (v2.04) ...
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bioRxiv - Microbiology 2021Quote: ... Three replicates of 150 µL of each sample was added to a 96-well plate and incubated with moderate shaking at 37°C (BioTek Synergy 4 Plate Reader) and the OD600 was measured at 30 min intervals for 16 h ...
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bioRxiv - Microbiology 2020Quote: ... Frozen −80°C stocks of each pathogen were thawed and grown to early/mid exponential phase using a Cytation3 plate reader (BioTek Instruments Inc., Winooski, VT) and approximately 2×105 CFU/mL of pathogen was inoculated into 180 μL of sterile Luria Bertani (LB ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated for 24 h at 37 °C in the presence of 5% CO2.with an automated liquid dispenser (BioTek MicroFlo; Thermo-Fisher, Inc.) Drugs/chemicals were dosed in DMSO at 12 concentrations ranging from 50 pM to 50 μM (half-log dilutions ...
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bioRxiv - Microbiology 2020Quote: ... which were incubated during 22 h at 37 °C with shaking (250 rpm) in a plate reader (Synergy HTX Multi-Mode Reader, BioTek Instruments, Inc, VT, USA). Optical densities (OD ...
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bioRxiv - Synthetic Biology 2019Quote: ... to prevent evaporation of lactate esters and incubated at 37°C with continuous shaking using a BioTek Synergy HT microplate reader (BioTek Instruments, Inc., VT, USA). OD600nm was measured at 20 min intervals ...
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bioRxiv - Microbiology 2023Quote: ... Absorbance measurements were taken at 30 min intervals for 7 d at 600 nm under shaken conditions at 30°C (Biotek Synergy H1, Winooski, VT). The maximum growth rate was identified from the linear region of the kinetic curves.
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bioRxiv - Microbiology 2023Quote: ... Bacteria were then grown for 24 h at 30°C under continuous shaking in an Epoch 2 Microplate Spectrophotometer from BioTek (Santa Clara, CA, USA). The absorbance at O.D.660 nm was measured every 10 min.
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bioRxiv - Microbiology 2023Quote: ... the infected cells were transferred to a 96-well plate and incubated at 40 °C in a microplate reader and absorbance was measured every 15 minutes for 16 hours (BioTek Synergy HTX Multimode Reader). Time at maximum rate (t at max V ...
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bioRxiv - Bioengineering 2023Quote: ... This mixture was incubated at 37°C for 2 h and the absorbance was measured at 562 nm using a SYNERGY HTX multi-mode reader (BioTek Instruments Inc., Winooski, VT).
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bioRxiv - Synthetic Biology 2023Quote: ... The absorbance at 340 nm (A340) was monitored in 50 s-intervals at 30°C in an EPOCH2 microplate reader (BioTek Instruments, Winooski, VT, USA). Enzymatic activities were determined by comparison with the corresponding blank experiments ...
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bioRxiv - Bioengineering 2021Quote: ... Methods for aggregation assays for recombinant human insulin were adapted from Webber et al.11 Formulation samples were plated at 150 μL per well in a clear 96-well plate and an absorbance reading was taken at 540 nm (BioTek Synergy H1 microplate reader). The aggregation of insulin leads to light scattering ...
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bioRxiv - Neuroscience 2020Quote: ... contents were mixed on an orbital shaker for 2 minutes and luminescence readings from experimental samples and ATP standards were acquired using a Synergy H1 microplate reader (BioTek® Instruments, Inc., 253147) and Gen5 Software ...
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bioRxiv - Pathology 2021Quote: ... The total sugar and starch content was calculated by reading the absorbance at 630 nm in a microplate reader (BioTek Synergy H1. VT, USA) using a calibration curve performed with glucose.
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bioRxiv - Immunology 2019Quote: ... The plate absorbance was measured by reading the plates at 450 nm using a Multi Mode reader (BioTek™ Cytation™ 3; ThermoFischer Scientific).
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bioRxiv - Immunology 2023Quote: ... The decreased calcein fluorescence in target cells due to cell lysis was measured at 485 nm excitation wavelength and 528 nm emission wavelength in the bottom reading mode using a Synergy HTX multi-mode plate reader (BioTek, Santa Clara, California, USA). The fluorescence for the experimental condition was adjusted by the parameter γ according to the live target cell control fluorescence ...
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bioRxiv - Microbiology 2021Quote: ... medium (HuanKai Microbial, 024051) (28°C, shaken at 200 rpm under a normal aerobic atmosphere) and harvested at ∼0.8 OD600 (BioTek, Synergy H1 Hybrid Multi-Mode Reader). Bacterial cells were centrifuged at 5,000 ’g for 5 minutes ...
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bioRxiv - Microbiology 2021Quote: The lactobacilli growth rate analysis was performed at 30 and 37°C, under agitation (double orbital, fast mode) in Synergy H1 plate readers (Biotek Instruments, Inc. Winooski, VT, USA). OD was measured every 30 minutes for 24h ...
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bioRxiv - Microbiology 2020Quote: ... UC500) and growth at 37 °C was monitored by measuring optical density at 600 nm every 15 minutes (Biotek Eon instrument with a BioStack 4). Carbon sources tested include D-glucose ...
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bioRxiv - Microbiology 2020Quote: Pure cultures of defined mutants were diluted to A600 0.003 and grown +/− antibiotic in 96-well microtiter format at 37°C with shaking in a plate reader (Tecan M200 Pro, Biotek Epoch 2, or Biotek Synergy H2M). Growth was monitored as change in A600 ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μL of substrates was added and fluorescence (λex◻=◻365◻nm and λem◻=◻455◻nm) was read at 37°C in 1 min intervals on a Cytation 3 imaging reader (BioTek, Winooski, VT, USA) for 60◻min ...
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bioRxiv - Cancer Biology 2020Quote: ... Magnetic cell sorting was performed to separate purified human versus mouse cell fractions using the Mouse Cell Depletion Kit (Miltenyi Biotek, 130-104-694). For the proteomic experiments ...