Labshake search
Citations for Biotek :
401 - 450 of 459 citations for 2 Cyano 2' pyrrolidinomethyl benzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Serosal samples were collected every 30 minutes for 2 hours with FITC dextran flux (ng/ml/cm2/hr) determined by plate reader (Biotek Synergy H1).
-
bioRxiv - Immunology 2021Quote: ... and light emission was measured every 2 minutes for a period of 120 min using the Synergy H1 microplate reader (Biotek Instruments, USA). The light emission levels are expressed as RLU (Relative Light Units = luminescence measured by the luminometer).
-
bioRxiv - Plant Biology 2022Quote: ... The DNA was normalized to 5 ng/μL based on quantification with a Synergy 2 multimode microplate reader (Biotek Instruments, Winooski, VT) with the Quant-iT PicoGreen double-stranded DNA quantification assay (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... were incubated in a 96-well flat-bottom plate (Nunclon) at 37°C with continuous shaking (double orbital) in a microplate spectrophotometer (BioTek Epoch 2). Optical densities were recorded every 20 min ...
-
bioRxiv - Plant Biology 2022Quote: ... The reaction was followed by measuring the absorbance at 363 nm at 25°C for 15 min using a plate reader (BioTek Synergy 2). For NR activity measurements on material extracted from Arabidopsis ...
-
bioRxiv - Cancer Biology 2022Quote: ... and day 2 by addition of the CTG reagent followed by measurement of luminescence using the Biotek Cytation 5 (BioTek, Winooski, VT). Luminescence values were adjusted based on 2µM Adenosine triphosphate (ATP ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The O.D./min was continuously recorded at 405 nm over 30 min to measure the hydrolytic product 5-mercapto-2-nitrobenzoic acid using a Synergy H1 microplate reader (Biotek; VT, USA). O.D./min was converted to catalytic rate using the Beer-Lambert law:
-
bioRxiv - Plant Biology 2023Quote: ... The background luminescence of seedling was recorded for 2 min before application of flg22 and chitin (5 s for NaCl) on a microplate reader (BioTek Synergy H1). Then 50 μl of 200 nM flg22 ...
-
bioRxiv - Immunology 2023Quote: ... This was achieved by collecting samples from the serosal compartments every 30 minutes for 2 hours and measuring the respective FITC concentrations on a plate reader (Synergy H1; BioTek, Winooski, VT). Samples with visible FITC leakage in the serosal compartment were omitted ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The absorbance at 405 nm was measured every 1 min for at least 30 min and up to 2 h using a Synergy 4 microplate reader (Biotek, Winooski, VT). SEAP activities were calculated as a function of the increase in absorbance at 405 nm over time42.
-
bioRxiv - Genetics 2023Quote: ... The plates were sealed with clear plastic adhesive film and incubated at 30°C with no shaking in a microplate spectrophotometer (BioTek Epoch 2) with OD600 readings taken of each well every 30 minutes for 48 hours ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... were added to wells in a microplate and incubated for 2 min at 37°C in a microplate reader (Cytation 3 Imaging Reader, BioTek, VT, USA). Then ...
-
bioRxiv - Microbiology 2024Quote: ... OD660 nm was recorded every 10 min for 24 h at 30 °C under continuous shaking in an Epoch 2 absorbance reader (Biotek Instruments Inc.). When required ...
-
bioRxiv - Microbiology 2024Quote: ... the OD600 and the relative fluorescence units (RFU) of the cultures were recorded hourly using a Synergy 2 Multi-Mode reader (BioTek, Winooski, VT) and the RFU results were normalized against the OD600 of the culture (82).
-
bioRxiv - Neuroscience 2024Quote: ... ELISA plates were read with a Synergy 2 Gen 5 microplate reader at 405 nm absorbance (BioTek Instruments Inc., Winooski, VT, USA).
-
bioRxiv - Cell Biology 2024Quote: ... and the samples’ optical density (OD) was measured in a 96 wells plate at 540 nm using a spectrophotometer (Epoch 2, BioTek, Winooski, USA). ODs were corrected by the total number of cells counted (Countess 3 Cell Counter ...
-
bioRxiv - Cell Biology 2020Quote: ... The purified extracts of CanlonicSF were excited at 485 ± 20 nm and the intensity of fluorescence emission of cpGFP was recorded at 530 ± 15 nm using the multiwell plate analyzer Synergy 2 (Biotek, Winooski, Vermont, USA). Excitation and emission spectra were obtained by collecting the emissions at 515 ± 15 nm using a multiwell plate reader EnVision (PerkinElmer ...
-
bioRxiv - Immunology 2022Quote: ... Reactions were stopped by the addition of 2M sulfuric acid and read using a BioTek Synergy 2 or Synergy H1 plate reader set at 450nm (BioTek, Winooski, VT, USA). Receiver operator curve (ROC ...
-
bioRxiv - Physiology 2020Quote: ... The absorbance was determined spectrophotometrically at a wavelength of 450 nm using a spectrophotometer (Epoch 2 microplate spectrophotometer, BioTek Instruments, Inc, Vermont, USA).
-
bioRxiv - Biochemistry 2021Quote: We measured the catalytic activity of the enzyme citrate synthase in mitochondrial samples monitoring the conversion of oxaloacetate and acetyl-CoA to citrate and CoA– SH and by measuring the formation of the colorimetric product thionitrobenzoic acid (TNB) at 412 nm and 37°C (Shepherd and Garland, 1969) on a microplate reader (Power Wave XS-2, Biotek Instruments, Winooski, USA). We calculated the enzyme activity using the changes in absorbance after substrate (250 µM oxaloacetate ...
-
bioRxiv - Neuroscience 2020Quote: ... Fluorescence post-thallium addition was monitored every 21 seconds for 600 seconds total using a BioTek Synergy 2 (BioTek, Winooski, VT, United States) multi-well plate reader equipped with an excitation filter of 485/20 nm and emission filter 528/20 nm ...
-
bioRxiv - Microbiology 2022Quote: ... The cells were grown with agitation at 30°C for 30 hours in a microplate reader (Infinite M Plex; Tecan, Austria, or Synergy 2; BioTek, Winooski, VT, USA), and the optical density at 600 nm (OD600 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Aerobic growth was monitored in technical duplicates or triplicates at 37°C in a BioTek Epoch 2 Microplate Spectrophotometer (BioTek, Bad Friedrichshall, Germany) by absorbance measurements (600nm ...
-
bioRxiv - Microbiology 2021Quote: ... the cells were collected by centrifugation at 10,000 g for 5 min and the absorbance of the supernatant was measured at 562 nm with a Synergy 2 spectrophotometer (BioTek, Winooski, VT, USA). Quantities of ferrihydrite reduced were determined using a standard curve containing a 2-fold range of FeSO4 (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2021Quote: ... was grown at 27°C on a 96-well plate (200 μL) and absorbance at 600 mm was recorded on a microplate reader (Synergy 2 Multi-Mode Microplate Reader, BioTek, Winooski, Vermont, USA). Non-inoculated media were used as blank ...
-
bioRxiv - Cell Biology 2021Quote: ... was measured every 5 min for 2 h at 25°C using Synergy H1 Hybrid Multi-Mode Microplate Reader (BioTek, cat. no. H1MFDG). The rate of kinetic reaction (slope ...
-
bioRxiv - Immunology 2020Quote: ... Growth was measured as relative light units at 528 nm after excitation at 485 nm in a fluorescence microplate reader (Synergy 2, BioTek Instruments, Vermont, USA) at the indicated time points.
-
bioRxiv - Genomics 2021Quote: ... absorbance was measured for each well at 435 nm and 545 nm on a Synergy 2 plate reader (Biotek Instruments, Winooski, VT, USA). Because of variation in background absorbance from well to well on each plate ...
-
bioRxiv - Genetics 2020Quote: ... Luciferase activity was determined by analyzing 50 μl of each cell extract in a luciferase assay system according to the standard protocol for the Synergy 2 Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA).
-
bioRxiv - Evolutionary Biology 2022Quote: ... The OD600 was measured every 2 hours for 24 hours at 37°C and slow continuous shaking using a plate reader (Synergy HT BioTek, Winooski, VT, USA). We used population growth rate and yield as fitness measures (Chavhan et al. ...
-
bioRxiv - Synthetic Biology 2022Quote: ... of each well every ∼10 minutes with intermittent orbital and linear shaking in a BioTek Epoch 2 plate reader (BioTek, Bad Friedrichshall, Germany). As had previously been established empirically for the instrument ...
-
bioRxiv - Physiology 2024Quote: ... Cells were then loaded into individual wells of a white 96-well luminescence plate with an automatic injector unit and luminescence was measured for 20 seconds using a Synergy 2 Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA). Each well of the 96-well plate was pre-loaded with candidate ligands (recombinant ITPa ...
-
bioRxiv - Zoology 2024Quote: ... and the luminescent response was measured for 20 seconds followed by additional measurements for 10 seconds after ATP injection to the same well with a Synergy 2 Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA).
-
bioRxiv - Immunology 2023Quote: ... AP-conjugated secondary reagents were detected using Lumi-Phos (Lumigen, Southfield, Michigan, USA; 33% solution in water) and a Synergy 2 Luminometer (BioTek, Winooski, Vermont, USA). Washing steps were performed on an ELx405 Select Deep Well Microplate Washer (BioTek ...
-
bioRxiv - Biophysics 2022Quote: ... The amount of inorganic phosphate (Pi) at each time point was determined by taking the absorbance at 620 nm using a plate reader (BioTek Synergy Neo 2) and comparing to a standard Pi concentration plot.
-
bioRxiv - Biochemistry 2023Quote: ... The plates were incubated in the multi-well plate reader instruments (Tecan – Spark/infinite, Crailsheim, Germany or BioTek Epoch 2 - BioTek, Bad Friedrichshall, Germany) at 30 °C ...
-
bioRxiv - Microbiology 2024Quote: ... removal by vegetative cells was measured by sampling supernatant and assaying AcA using the change in absorbance at 380 nm in the presence of 2--amino benzamidoxime (ABAO), (as inspired by aldehyde quantifications by Ressmann et. al, 2019) using an Epoch microplate reader (BioTek; Gen5 v3.10 software) [42] ...
-
bioRxiv - Physiology 2024Quote: ... The increase in absorbance at 412 nm was monitored over time using a microplate reader (PowerWave XS 2, BioTek Instruments, Winooski, VT, USA). This increase in absorbance indicates the formation of thionitrobenzoic acid ...
-
bioRxiv - Microbiology 2024Quote: ... FITC concentrations on the basolateral side were quantified by measuring the fluorescence intensity (485 nm excitation and 530 nm emission) in a microplate reader (Synergy-2, BioTek, Winooski, VT, USA). FITC levels were calculated using a standard curve of FITC in DMEM with the indicated supplements ...
-
bioRxiv - Plant Biology 2024Quote: Total chlorophyll was extracted from 20 mg frozen powder with 2 ml methanol and quantified spectrophotometrically at 652 nm and 665 nm with a Synergy HTX microplate reader (BioTek, Bad Friedrichshall, DE). Chlorophyll content was calculated using the equation ...
-
bioRxiv - Microbiology 2021Quote: ... flat-bottomed 96-well plate and the optical density values were read at 726nm on an Epoch 2 BioTek plate reader (BioTek Instruments, Winooski, VT, USA). The standard curve equation was used to determine total polyphenol content of each sample based on absorbance values.
-
bioRxiv - Microbiology 2020Quote: ... transferred to 24-well culture plate and the OD600 determined kinetically (1 point/h) using a BioTek Synergy 2 spectrofluorometer (BioTeK U.S., Winooski, VT, USA) at 25°C with continuous slow agitation ...
-
bioRxiv - Neuroscience 2020Quote: ... contents were mixed on an orbital shaker for 2 minutes and luminescence readings from experimental samples and ATP standards were acquired using a Synergy H1 microplate reader (BioTek® Instruments, Inc., 253147) and Gen5 Software ...
-
bioRxiv - Cell Biology 2022Quote: ... Samples from basolateral chambers were collected at 1 and 2 hours after the start of the experiment and signals from the samples were read by a fluorimeter (BioTek Instruments, Winooski, VT, USA) to determine the amount of dextran passing through the epithelial monolayers.
-
bioRxiv - Physiology 2022Quote: ... All assays were run in duplicate under control conditions or in the presence of 5 mM DTT in a 96-well plate and changes in absorbance were tracked using a microplate reader (Synergy 2, BioTek/Agilent, Santa Clara, CA). This concentration was selected based on similar experiments in a previous study [15] ...
-
bioRxiv - Physiology 2020Quote: ... The absorbance change vs time for each biomarker was measured spectrophotometrically at a specific wavelength using a spectrophotometer (Epoch 2 microplate spectrophotometer, BioTek Instruments, Inc, Vermont, USA).
-
bioRxiv - Microbiology 2023Quote: ... prior to reading under OD885 against the standard solutions of potassium dihydrogen phosphate (0-2 mg/L) using a microplate reader (EPOCH2, Biotek, Santa Clara, California, USA).
-
bioRxiv - Molecular Biology 2024Quote: ... 2-4 images at the central callus and distal callus regions were taken using an automatic microscope (Agilent, BioTek Lionheart FX, Santa Clara, CA). Central callus regions were defined as proximal to the fracture site on either side of the bone ...
-
bioRxiv - Biochemistry 2023Quote: ... 250 μl ABTS was mixed with 10 μl of the sample (ethanol and GD extracts) and measured at the wavelength 724 nm using UV/Vis microplate reader (Epoch 2 Microplate Spectrophotometer, BioTek Instruments, Winooski, Vermont, USA). after 15 min of incubation in RT ...
-
bioRxiv - Biochemistry 2023Quote: TPC analyses were carried out with the protocol of Singleton and Rossi (Singleton & Rossi, 1965) adopted for microplate reader (Epoch 2 Microplate Spectrophotometer, BioTek Instruments, Winooski, Vermont, USA). Ten microliters of extract ...