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Citations for Biotek :
251 - 280 of 280 citations for Dog Trefoil Factor 3 TFF3 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Low-affinity complexes and unbound proteins were removed in consecutive washing steps with PBS-T 0.05% (EL406 washer, BioTek). Beads were incubated for 10 minutes with 0.4% paraformaldehyde ...
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bioRxiv - Bioengineering 2020Quote: ... 5% CO2 using an automated digital microscope with a 10× objective with a gas controller (Cytation 3 microplate reader, BioTek Instruments Inc., Winooski, VT, USA). Images were collected every 10 minutes for 24h ...
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bioRxiv - Cancer Biology 2020Quote: ... was added and intensities were measured for each well using a Cytation 3 Multi Modal Reader with Gen5 software (BioTek Instruments, Inc., Winooski, VT, USA). Along with cell viability ...
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bioRxiv - Biochemistry 2024Quote: The pyrene–actin based bulk–polymerization experiments in the absence and presence of VCA– activated Arp2/3 complex were performed at a Synergy 4™ microplate reader (BioTek Instruments, Winooski, VT, USA) using the built–in filter set (Excitation ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined by taking OD at 280 nm in Nano drop (Synergy H1 Hybrid Multi-Mode Reader Biotek) and mass extinction co-efficient of the proteins.
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bioRxiv - Neuroscience 2021Quote: ... The solubilized protein was quantified by BCA Assay Kit (Fisher) in triplicates at 590nm using a microplate reader (Synergy HT, BioTek). 15 to 20 µg of ß-mercaptoethanol denatured protein were loaded in Tris-glycine polyacrylamide gels (14% ...
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bioRxiv - Microbiology 2020Quote: The TreS activity domain of TreS-Mak protein was monitored following the conversion of trehalose into maltose and glucose using Epoch spectrophotometer (Biotek). 15µL of reaction sample were incubated 30 min at 58°C with 30 µL of 100 mM sodium citrate pH 4.6 containing whether 0.4 U of amyloglucosidase from Aspergillus niger (Megazyme ...
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bioRxiv - Neuroscience 2020Quote: ... All assays were allowed to incubate at 37°C for 30 min before protein concentration was read in at 562 nm (SynergyMix, Biotek).
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bioRxiv - Cancer Biology 2022Quote: ... Cell and tissue debris was removed by centrifugation at 16,000x g for 5 minutes at room temperature and protein concentration was measured using A280 values determined by Cytation 5 (BioTek). Lysates were normalized in Buffer A with 1% SDS and prepared with 6X Laemmli sample buffer containing 100 mM 2-mercaptoethanol (Sigma M6250) ...
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bioRxiv - Microbiology 2023Quote: ... Fluorescence of the Amcyan and Turborfp proteins produced by the spot colonies from each well was measured using the Synergy H1 plate reader (BioTek). We used the constitutive fluorescence of Amcyan to normalize TurboRFP production by reporter expression ...
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bioRxiv - Microbiology 2023Quote: ... The green fluorescent protein (GFP)-positive cells were then counted using Cytation 5 with Gen5 v3.03 software (BioTek, Winooski, VT). Dose-response curves were plotted and EC50 values determined using Prism 9 (GraphPad ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell debris was removed by centrifugation at 16,000x g for 5 minutes at room temperature and protein concentration was measured using A280 values determined by Cytation 5 (BioTek). Lysates were normalized in Buffer A with 1% SDS and prepared with 6X Laemmli sample buffer containing 100 mM 2-mercaptoethanol (Sigma M6250) ...
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bioRxiv - Microbiology 2024Quote: ... Samples were incubated at 95°C for 5 minutes and proteins were quantified using tryptophan fluorescence in a Synergy H1M microplate reader (BioTek) with an excitation wavelength of 280 nm and an emission wavelength of 360 nm ...
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bioRxiv - Cell Biology 2023Quote: ... the fluorescence was measured at 340 nm excitation wavelength and 480 nm emission wavelength in a Cytation™ 3 microplate reader (BioTek Instruments Inc., Winooski, VT, USA).
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bioRxiv - Cell Biology 2023Quote: ... The fluorescent signal was measured at 548 nm excitation wavelength and 574 nm emission wavelength in a Cytation™ 3 microplate reader (BioTek Instruments Inc., Winooski, VT, USA). As a positive control ...
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bioRxiv - Neuroscience 2021Quote: ... Plasma osmolality was analyzed by vapor pressure osmometry (VAPRO 5520) and volemia was indirectly inferred by the plasma protein concentration which was measured in an absorbance microplate reader (BioTek EL800), according to the protocol proposed by Lowry et al ...
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bioRxiv - Synthetic Biology 2022Quote: The fluorescence intensity of the synthesized fluorescent proteins was measured by the multi-well plate fluorometer (Synergy HTX, BioTek, Winooski, VT). 5 µL of the cell-free synthesized fluorescent protein and 45 µL of Milli-Q water were mixed in a 96-well half-area black plate (Corning Incorporated ...
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bioRxiv - Biophysics 2023Quote: Assessment of the extent of protein oligomerization was based on monitoring absorbance at 550 nm using BioTek Plate Reader (Agilent BioTek NEO2) to reflect turbidity changes induced by protein oligomer formation ...
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bioRxiv - Neuroscience 2024Quote: ... with Coomassie Blue-protein interaction assessed by absorbance at 595 nm using a microplate spectrophotometer (BioTek, Vermont, USA, VT 05404-0998). Sample concentrations were then adjusted to 1000 or 2000 µg/ml for Western blot analysis based on total protein yield ...
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bioRxiv - Cancer Biology 2024Quote: ... and the resulting supernatants were retained for protein quantification using a standard BCA protocol (Pierce BCA kit 23225) and a microplate reader (Biotek, Epoch2).
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bioRxiv - Bioengineering 2020Quote: ... Red fluorescent protein (RFP) readings (excitation 580 nm, emission 610 nm) were taken using a BioTek Synergy H1m plate reader (BioTek, Winooski, VT) after 18 hours of incubation at 37 °C with 200 rpm shaking.
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bioRxiv - Microbiology 2022Quote: ... The absorbance of each well with reaction mixture and protein (but without formaldehyde) was measured at 340 nm with a BioTek Synergy H1 plate reader (BioTek, Winooski, VT) in a Corning CoStar 48 well plate (Corning ...
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bioRxiv - Neuroscience 2022Quote: ... which used bovine serum albumin standards to create a best fit linear regression line based on protein concentrations using the 562nm absorbance mode of a microplate reader and its Gen5 Data Analysis program (Biotek, Winooski, VT). Equal amounts of protein were resolved in SDS-polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... which used bovine serum albumin standards to create a best fit linear regression line based on protein concentrations using the 562nm absorbance mode of a microplate reader and its Gen5 Data Analysis program (Biotek, Winooski, VT). Equal amounts of protein were resolved in SDS-polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 mM Tris was used to extract protein-bound dye and optical density was measured with a BioTek Synergy HT plate reader (SN 269140, BioTek Instruments Inc.) at 490 and 540 nm ...
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bioRxiv - Synthetic Biology 2023Quote: The relative fluorescence units (RFU) of the synthesized fluorescent proteins were measured by the multi-well plate fluorometer (Synergy HTX, BioTek, Winooski, VT). 5 μL of the cell-free synthesized fluorescent protein and 45 μL of Milli-Q water were mixed in a 96-well half-area black plate (Corning Incorporated ...
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bioRxiv - Immunology 2022Quote: ... Recombinant protein was checked for protein concentration by OD 260/280/320 using a Take3 micro-volume plate with a SynergyMX plate reader (BioTek, Winooski, VT, USA). NEU3 is 51 kDa ...
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bioRxiv - Immunology 2020Quote: ... Buffer-exchanged preparations were then checked for protein concentration by OD 260/280/320 using a Take3 micro-volume plate with a SynergyMX plate reader (BioTek, Winooski, VT, USA) as previously described (54) ...
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bioRxiv - Neuroscience 2023Quote: ... and protein concentrations were determined using the Bradford Assay and an Epoch BioTek Microplate Spectrophotometer (Gen 5 2.01; BioTek Instruments Inc; Winooski, VT). 25 μg of protein from tissue homogenates were combined with specific cytokine antibody-immobilized beads ...
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bioRxiv - Evolutionary Biology 2024Quote: Total host protein was quantified using the Bradford method (75) with absorbances read at 595 nm on a microplate reader (Biotek Synergy H1; CA, USA). Data were converted from absorbance to total protein concentrations (μg μL-1 ...