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Citations for Biotek :
201 - 250 of 335 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... the reaction was stopped with 50 μls of 3M hydrochloric acid and absorbance was measured at 490 nm (OD490) using a Synergy 4 (BioTek) plate reader ...
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bioRxiv - Cancer Biology 2023Quote: ... incubated for 4 h and fluorescence was read at 550 nm/590 nm on a Cytation 3 Imaging Reader (BioTek).
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bioRxiv - Microbiology 2023Quote: ... supernatant or size exclusion purified vesicles were extracted with butanol (1:2) overnight at 4°C and the butanol fraction transferred to microtiter plates for absorbance measurement (Synergy, Biotek).
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bioRxiv - Microbiology 2022Quote: ... Fluorescence of released 4-methylumbelliferone (4MU) was measured (Excitation 360 nm, Emission 450 nm) in a plate reader (Synergy MX, Biotek). One unit of chitinase activity releases 1 μmol of 4MU from the appropriate substrate per minute ...
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bioRxiv - Bioengineering 2022Quote: ... Pellet was then resuspended in 1 mL of M9 medium and flasks were inoculated to the starting OD600 of 0.1 and incubated at 30 °C with agitation (233 RPM for cultures with substrate concentration 4 g/L and 275 RPM for cultures with substrate concentration 8 g/L) using NB205 orbital incubator (N-Biotek). The ratio of medium to flask volume was 1:5 (20 mL:100 mL ...
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bioRxiv - Neuroscience 2023Quote: ... Absorbance at 490 nm was measured 4 hours after the addition of the MTS dye using a SynergyNEO2 plate reader (BioTek).
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bioRxiv - Physiology 2023Quote: ... The cells are first washed from their culture medium by 4 washes of KRB-BSA with the EL406 washer distributor liquid handling device (Biotek) suitable for 384-well plates ...
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bioRxiv - Microbiology 2023Quote: ... The reading process for multiple plates is automated using a BioStack™ 4 microplate stacker (BioTek Instruments, Inc., Winooski, VT), which cycles the experimental 96-well microplates and removes the lids at the specified interval as to not interfere with spectrophotometric values ...
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bioRxiv - Immunology 2024Quote: ... Plates were developed for 10 minutes at room temperature and then the reaction was stopped by adding 50 μL 3 M hydrochloric acid (HCl, Fisher) and plates were read using a Synergy 4 (BioTek) plate reader at an optical density (OD ...
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bioRxiv - Microbiology 2024Quote: ... Plates were stacked without lids and the OD600 was recorded every hour for 24 hours using a stacker (Biostack 4, Agilent BioTek) and plate reader (Epoch 2 ...
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bioRxiv - Microbiology 2024Quote: ... at 37 °C with aeration at 200 rpm until they reached an OD600 between 0.8 and 1.0 (assessed using spectrophotometer BioTek Synergy 4). In the case of low OD600 ...
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bioRxiv - Microbiology 2024Quote: ... then samples were diluted (1:4) in PBS and quantified by measuring OD595 using a Synergy neo2 plate reader (BioTek).
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bioRxiv - Neuroscience 2024Quote: ... After 40 hours post-transfection, cells were fixed in 4% PFA, and images (eGFP, mCherry, and eBFP2) were acquired using cytationC10 (Biotek) with a 20x objective ...
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bioRxiv - Microbiology 2024Quote: ... Plates were stacked without lids and incubated at 37 °C for 24 h in a stacker-incubator system (Biostack 4, Agilent BioTek) connected to a plate reader (Epoch 2 ...
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bioRxiv - Cancer Biology 2022Quote: ... Images were taken on day 10 using Cytation 5 Imaging Multi-Mode Reader (Biotek®). Images were further analyze using ImageJ ...
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bioRxiv - Neuroscience 2024Quote: ... and imaged via confocal microscopy (Cytation 10, Agilent BioTek, 20x objective, z-stack tile-scans). In Fiji ...
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bioRxiv - Immunology 2022Quote: ... Endpoint titers were determined by 4-parameter analysis using the Gen5™ 2.0 Data Analysis software (BioTek Instruments, Inc., Winooski, VT) and defined as the reciprocal of the serum dilution producing a signal identical to that of negative control samples at the same dilution plus three times the standard deviation.
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bioRxiv - Neuroscience 2021Quote: ... Uptake of MEs labeled with FM 4-64 and RNA cargos labeled with ExoGlow-RNATM was quantified by using a microplate fluorescence reader (BioTek Instruments, Inc.) and confocal microscopy imaging respectively ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 11.1 μl of Alamar blue reagent was added and the readings were taken after 4 hours in a Synergy H1 plate reader (BioTek, Winooski, VT) with 560 nm (excitation ...
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bioRxiv - Microbiology 2021Quote: ... 4 and 5 were performed using two plate readers: A Synergy Neo2 and a Synergy H1 (both from Biotek Inc., VT, USA). Both were set to 30°C with continuous shaking at an orbital displacement of 1 mm and a speed of 807 rpm ...
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bioRxiv - Microbiology 2023Quote: ... The resulting 1,472 SICs were arranged in 16 96-well plates and incubated in the anaerobic chamber at 37 °C (by setting the temperature of the chamber to 37 °C) in a plate stacker (BioTek BioStack 4) with 2 control plates containing uninoculated media at the top and bottom of the stack to minimize edge effects ...
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bioRxiv - Neuroscience 2023Quote: ... After 40 hours, cells were fixed in 4% PFA and images (eGFP, mCherry and eBFP2) were acquired at cytationC10 (Biotek, Objective 60x). eGFP (interaction signal ...
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bioRxiv - Microbiology 2022Quote: ... The plate was incubated with shaking at 37 °C and the optical density of each well was measured at a wavelength of 600 nm (BIOTEK, Synergy 4). The MIC values were taken as the lowest concentration for which no growth was discernible (<0.05 OD600 ...
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bioRxiv - Neuroscience 2023Quote: ... and fluorescence emitted by Atto488 (λex = 485 nm, λem = 538 nm) was measured in a Synergy 4 plate reader (BioTek Instruments GmbH) at intervals of 10 seconds ...
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bioRxiv - Microbiology 2024Quote: ... NBT was reduced to blueformazan and spectrophotometrically read at OD 650 nm using a Synergy 4 microplate reader (BioTek, Santa Clara, USA). The concentration at which the drugs were able to inhibit 50% parasite growth (IC50 ...
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bioRxiv - Microbiology 2023Quote: ... was recorded every 1 min over a period of 4 hours at 37°C using the Cytation 5 microplate reader (BioTek Instruments, USA).
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bioRxiv - Synthetic Biology 2023Quote: ... The absorbance at 405 nm was measured every 1 min for at least 30 min and up to 2 h using a Synergy 4 microplate reader (Biotek, Winooski, VT). SEAP activities were calculated as a function of the increase in absorbance at 405 nm over time42.
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bioRxiv - Plant Biology 2024Quote: ... Soil NH + and NO3- concentrations were analyzed in extracts from 20 g soil mixed with 80 mL 0.5M K2SO4 solution (1:4, soil: 0.5M K2SO4 solution) and measured calorimetrically at 650 nm on a µQuant microplate reader (BioTek, Winooski, VT, USA) following Sims et al ...
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bioRxiv - Biochemistry 2020Quote: ... Readings were taken once every 10 seconds and analyzed by the Gen5 Data Analysis Software (BioTek). (3 ...
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bioRxiv - Immunology 2024Quote: ... FISH slides were visualized and images collected on a Cytation 10 multimode plate reader (Agilent Biotek) using the 4X magnification lens ...
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bioRxiv - Cell Biology 2022Quote: ... We also performed immunofluorescence staining for pluripotency markers in these iPSC colonies using Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit (Thermo Fischer Scientific) as per manufacturer’s instruction and fluorescence were imaged using fluorescence microscopy (BioTek Lionheart LX Automated Microscope) as described previously.15 Alkaline phosphatase (ALP ...
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bioRxiv - Cell Biology 2021Quote: ... Absorbances were obtained at 620 nm and sample concentrations were determined by comparison to the CXCL12 standards using a 4-parameter curve fit (Synergy HT & Gen5 Software, BioTek Instruments, Winooski, VT). Serum level of CXCL12 was assessed using undiluted serum isolated from the blood of tumor-bearing mice or stage IV glioma patients.
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bioRxiv - Cancer Biology 2023Quote: ... incubated for 4 h and fluorescence was read at 550 nm/590 nm on a Cytation 3 Imaging Reader (BioTek, Winooski, Vermont, USA).
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bioRxiv - Evolutionary Biology 2022Quote: ... and Abs750nm of all 42 plates was measured after 65h exposition (4-6 hours after light on) with an absorbance microplate reader Epoch (BioTek Instruments, VT, USA). 9 data points per well were analyzed and averaged using software Gen5 2.0 (Biotek ...
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bioRxiv - Cancer Biology 2024Quote: ... Luminescence was read every 10 min for 1h using Cytation 5 microplate reader and Gen5 software (BioTek). Relative NAD(H ...
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bioRxiv - Microbiology 2024Quote: ... 450 nm) from 10 min to 45 min was obtained by the plate reader (Synergy HTX, BioTek) and used as the NA activity measured by MUNANA assay ...
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bioRxiv - Molecular Biology 2023Quote: ... Fluorescence was measured at 10 seconds at Relative Luciferase Units (RLU) using a luminometer microplate reader (Biotek synergy H1 hybrid plate reader with Gen5v ...
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bioRxiv - Microbiology 2024Quote: ... cultures were diluted 1:10 and final OD600 was measured using an Epoch 2 plate reader (Biotek). Isolates were mixed with glycerol (final concentration of 12% ...
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bioRxiv - Microbiology 2021Quote: ... Three replicates of 150 µL of each sample was added to a 96-well plate and incubated with moderate shaking at 37°C (BioTek Synergy 4 Plate Reader) and the OD600 was measured at 30 min intervals for 16 h ...
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bioRxiv - Molecular Biology 2024Quote: ... 2-4 images at the central callus and distal callus regions were taken using an automatic microscope (Agilent, BioTek Lionheart FX, Santa Clara, CA). Central callus regions were defined as proximal to the fracture site on either side of the bone ...
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bioRxiv - Microbiology 2023Quote: ... We performed PCA oxidation assays either by tracking the fluorescence of reduced PCA over time in a plate reader (BioTek Synergy 4 or HTX) housed in a Coy-brand anoxic chamber or by tracking the current generated by a culture when provided PCA and an electrode poised to continuously reduce any PCA that the cells oxidized ...
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bioRxiv - Biochemistry 2020Quote: ... In the linear phase of the reaction (10 – 40 min) turnover rates were calculated using Gen5 software (BioTek) as RFU/min and were normalized by subtracting background hydrolysis rates measured for each substrate in reaction buffer in the absence of protein.
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bioRxiv - Immunology 2022Quote: ... 1:10) were prepared in PBS and the absorbance was measured at 380 nm (Epoch Microplate Spectrophotometer, BioTek) to calculate the amount needed to make the required dose using the bacterial growth standard curve.
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bioRxiv - Immunology 2020Quote: ... unspecific protein binding sites were blocked by incubating sections with PBS containing 10% normal donkey serum (Pan-Biotek), 2% BSA and 0.2% Triton-X100 for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... Whole-miBrain images were collected after 24 hours (Cytation 10, Agilent BioTek, 4x objective, z-stack tile-scans). In Fiji ...
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bioRxiv - Immunology 2022Quote: ... Reactions were stopped by adding 50 μL 3M hydrochloric acid and absorbance at 492 nm was determined on a Synergy 4 plate reader (BioTek, Agilent Technologies inc., CA, USA) or similar ...
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bioRxiv - Biochemistry 2024Quote: The pyrene–actin based bulk–polymerization experiments in the absence and presence of VCA– activated Arp2/3 complex were performed at a Synergy 4™ microplate reader (BioTek Instruments, Winooski, VT, USA) using the built–in filter set (Excitation ...
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bioRxiv - Biochemistry 2024Quote: The polymerization and depolymerization of pyrene–labeled recombinant WT or mutant actin (5% pyrene-labeled WT or mutant actin) was monitored as a function of increasing or decreasing pyrene– fluorescence using a Synergy 4™ microplate reader (BioTek Instruments, Winooski, VT, USA) and the built–in filter set (Excitation ...
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bioRxiv - Neuroscience 2021Quote: ... λem= 490 nm was measured every 10 min for up to 24 h using a fluorescence plate reader (Biotek). Once the optimal concentrations of approximately 20 μM Aβ42 ...
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bioRxiv - Microbiology 2022Quote: ... The growth was assessed by measuring the OD600 every 10 minutes for 72 hours using a microplate reader (Biotek synergy H1 ...