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Citations for Biotek :
201 - 250 of 276 citations for Rat Small Nuclear Ribonucleoprotein U5 Subunit 200 SNRNP200 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and U937 cells using the Omega Biotek E.Z.N.A Total RNA Kit I (Omega Biotek, R6834-02). For 3D cell extractions ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was isolated from exponentially growing cells via E.Z.N.A total RNA kit I (Omega BioTek) and converted to cDNA with high-capacity cDNA Reverse Trancriptase kit (Applied Biosciences) ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using a modified version of the EZNA Bacteria Kit (Omega Biotek, Norcross, GA). Microbial RNA was extracted using a modified version of the HP Total RNA Kit (Omega Biotek ...
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bioRxiv - Cancer Biology 2022Quote: ... and plasmid DNA was isolated using OMEGA’s EZNA plasmid DNA miniprep kit (Omega Biotek Inc Cat# D694202). Lentivirus containing supernatants were produced in packaging (293T ...
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bioRxiv - Genomics 2022Quote: Cell-free DNA was extracted from plasma using the Magbind ccfDNA extraction kit (Omega Biotek cat#M3298). Manufacturer recommendations for extraction were followed ...
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bioRxiv - Immunology 2020Quote: ... Samples were analyzed by agarose gel electrophoresis and purified PCR products (ENZA cycle pure kit; Omega Biotek) were cloned into the pCR2.1 vector using the Original TA cloning kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Extracellular viral RNA was isolated from infected cell supernatant using the E.Z.N.A Viral RNA kit (Omega-BioTek). Cell-associated viral RNA was extracted from infected cells using the E.Z.N.A Total RNA kit (Omega-BioTek) ...
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bioRxiv - Microbiology 2023Quote: ... Cell-associated viral RNA was extracted from infected cells using the E.Z.N.A Total RNA kit (Omega-BioTek). Viral RNA was reversed transcribed and quantified by qPCR.
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bioRxiv - Genomics 2021Quote: Viral load quantification: viral RNA was extracted from Mucilair apical washes using EZNA viral extraction kit (Omega Biotek) and quantified by using RT-qPCR with the QuantiTect kit (#204443 ...
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bioRxiv - Microbiology 2021Quote: Samples were prepared and DNA was extracted using the E.Z.N.A Soil DNA kit (Omega Biotek, Norcross, GA, USA). The tips of the swabs were placed in disruptor tubes by using a sterilized scissor ...
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bioRxiv - Developmental Biology 2022Quote: ... total RNA from the embryos was extracted with an OMEGA Total RNA extraction kit I (Omega Biotek, USA). To remove genomic DNA ...
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bioRxiv - Biochemistry 2020Quote: ... The −500 to +216 region corresponding to the HSPA1B promoter was amplified from the plasmid by PCR with Phusion polymerase (Thermo-Fisher #F530S) and purified via E.Z.N.A Gel Extraction Kit (Omega BioTek #D2500). The DNA was ethanol precipitated ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were harvested three days post transfection using E.Z.N.A Total RNA Kit 1 (Omega Biotek cat #R6834-01) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: Genomic DNA was isolated from the tissue samples of Þistilfjörður population using the E-Z 96 Tissue DNA Kit (Omega Biotek) according to the manufacturer’s recommendation ...
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bioRxiv - Genomics 2023Quote: ... DNA was extracted from single adult weevils for each species using the EZNA Insect DNA Kit (Omega Biotek) and quantified using a Nanodrop 2000 ...
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bioRxiv - Microbiology 2024Quote: ... Microbial RNA was extracted using a modified version of the HP Total RNA Kit (Omega Biotek, Norcross, GA). Detailed protocols for DNA and RNA extractions can be found here ...
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bioRxiv - Synthetic Biology 2021Quote: ... Both fragments were cleaned with the Viogene Gel/PCR DNA Isolation Kit (Viogene-Biotek Corporation, New Taipei, Taiwan R.O.C.) and then fused in an overlapping PCR to make an IS1 fragment lacking inverted repeats but having SspI sites on the ends and an XhoI site in the center ...
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bioRxiv - Microbiology 2020Quote: Microbial DNA was extracted from the samples using an E.Z.N.A.® Stool DNA Kit (Omega BioTek, Norcross, GA, USA) according to manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2023Quote: ... the total RNA was extracted using the E.Z.N.A column-based total RNA kit (Omega BioTek, catalog number R6834-01) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: RNA was extracted from swab or tissue supernatant using the Mag-Bind Viral DNA/RNA 96 Kit (Omega Biotek) on the KingFisher FLEX System (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... and the band located at a 7.9 kb mark was cut and purified using the EZNA Cycle pure kit (Omega Biotek) Purified digested vector and inserts were ligated in a 1:30 vector/insert ratio using the T4 DNA ligase (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... DNA was immediately extracted from a portion of the zooplankton tissue mass via the EZNA tissue kit (Omega Biotek) and was used to create a highly concentrated standard whose DNA concentration was measured with a Qubit 2.0 (ThermoFisher) ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was extracted from frozen root material using the E.Z.N.A.® Plant RNA Kit with the associated RNase-free DNase I Set (Omega-Biotek, USA), following the standard protocol provided ...
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bioRxiv - Immunology 2024Quote: ... adherent and non-adherent cells were harvested and either lysed in TRK lysis buffer (Omega Biotek Total RNA kit) for RNA isolation or prepared for co-immunoprecipitation (below) ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 4-week-old Arabidopsis plants via a Plant RNA Kit (OMEGA Biotek, Norcross, USA). cDNA was synthesized via ReverTra Ace® qPCR RT Master Mix with gDNA Remover (TOYOBO) ...
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bioRxiv - Neuroscience 2021Quote: ... The solubilized protein was quantified by BCA Assay Kit (Fisher) in triplicates at 590nm using a microplate reader (Synergy HT, BioTek). 15 to 20 µg of ß-mercaptoethanol denatured protein were loaded in Tris-glycine polyacrylamide gels (14% ...
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bioRxiv - Microbiology 2020Quote: ... Each well was washed once with 1 mL 1X PBS and RNA was extracted using the EZNA Total RNA kit (Omega Biotek). 375 μL of TRK lysis buffer with β-Mercaptoethanol was added to each well and incubated for 3 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... or human IFN-β (1000 U/ml) and gaussia luciferase activity was determined using Gaussia Luciferase Glow Assay Kit (Thermo) on Cytation 3 (BioTek) multimode plate imager according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was isolated from clarified cell culture supernatants using an Omega Total RNA Isolation Kit 1 (Omega BioTek, VWR) and reverse transcribed at 42°C using an oligo dT primer and SuperScript II reverse transcriptase (Life Technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR products were purified by agarose gel and extracted using a GFX gel band extraction kit according to the manufacturer’s instruction (Omega Biotek, USA). 500ng of purified PCR fragments were used to prepare Digoxigenin or Fluorescein labelled antisense probes ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein quantification was performed using the Pierce BCA protein assay kit (ThermoScientific) and analyzed on a Cytation 3 plate reader (BioTek). Proteins were separated by NuPAGE 4-12% Bis-Tris Gel and transferred to a nitrocellulose membrane ...
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bioRxiv - Physiology 2021Quote: All serum insulin concentrations were quantified using a commercially available insulin immunoassay kit (32380; Toronto Bioscience) as per manufacturer’s instructions using Synergy H4 Hybrid microplate reader (BioTek Instruments). Insulin concentrations were interpolated from a standard curve determined by curve of 4-parameter as per manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: 72 hours after transfection cells were harvested and RNA extraction was performed using E.Z.N.A Total RNA Kit 1 (Omega Biotek cat #R6834-01). cDNA generation was performed using SuperScript IV Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: ... Oligos were converted to double-strand DNA using qPCR and purified on a spin column (EZNA Cycle Pure kit from Omega BioTek). The pSC101 backbone was amplified with two separate primer pairs encoding BsaI cut sites that matched the insertion location of the oligos on the TtgR gene ...
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bioRxiv - Microbiology 2022Quote: Total microbial DNA was extracted from the fecal samples using the OMEGA Soil DNA Kit (Omega BioTek, Norcross, GA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Leaf tissue was flash frozen in liquid nitrogen and RNA extracted using the EZNA Plant RNA Kit I (Omega Biotek). DNA contamination was removed by treating total RNA with Turbo DNase with inactivation reagent (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA was isolated from frozen liver samples and DNase treated using commercially available kits (Omega Biotek and Ambion, respectively). Libraries were constructed and subsequently subjected to 151 bp paired-end cycles on the NovaSeq-6000 platform (Illumina) ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA concentration measurements were performed using the Qubit dsDNA high sensitivity assay kit on the Synergy H1 Hybrid Microplate Reader (BioTek). Libraries were generated using the Nextera XT Library Prep kit (Illumina ...
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bioRxiv - Biochemistry 2022Quote: ... Oligonucleotides were converted to double-stranded DNA using 15 cycles of PCR amplification and purified on a spin column (EZNA Cycle Pure kit from Omega BioTek). A pSC101 backbone containing extant E ...
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bioRxiv - Immunology 2022Quote: ... and lambda chains.48 The products from the second PCR were analyzed by agarose gel electrophoresis and purified PCR products (ENZA cycle pure kit; Omega Biotek) were submitted to Genewiz for sequencing ...
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bioRxiv - Cell Biology 2022Quote: ... Lipid hydroperoxides were extracted from neuronal lysates and neuron-conditioned media and analyzed using a lipid hydroperoxide assay kit according to the manufacturers protocol and Synergy Mx Multi-Mode Microplate Reader (BioTek). VAMP7 and syntaxin 4 knockdown neurons used for the lipid hydroperoxide assay were transduced at an MOI 7.5.
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bioRxiv - Microbiology 2024Quote: DNA was extracted from 200µL aliquots of individual samples using the EZNA Bacterial DNA Kit (Omega Biotek, Norcross, GA, USA) with some modifications ...
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bioRxiv - Cell Biology 2024Quote: Total soluble RNA (≥18 nucleotides) was extracted from frozen cell pellets using the E.Z.N.A.® DNA/RNA Isolation kit (Omega BioTek: R6731-01) according to manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from pure cultures using the Omega Mag-Bind Universal Pathogen Kit according to manufacturer’s protocol with a final elution volume of 60µl (Omega Biotek Inc., Norcross, GA). DNA samples were quantified using Qubit fluorometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from pure cultures using the Omega Mag-Bind Universal Pathogen Kit according to manufacturer’s protocol with a final elution volume of 60μl (Omega Biotek Inc., Norcross, GA). DNA samples were quantified using Qubit fluorometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from pure cultures using the Omega Mag-Bind Universal Pathogen Kit according to manufacturer’s protocol with a final elution volume of 60μl (Omega Biotek Inc., Norcross, GA). DNA samples were quantified using Qubit fluorometer (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: Total RNA extraction from H4 cells was carried out using the E.Z.N.A.® Total RNA Kit I (Omega Biotek, Norcross, GA, USA), and either purity (260nm/280nm ratio and 260nm/230nm ratio ...
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bioRxiv - Plant Biology 2020Quote: ... and total RNA was extracted using the protocol for difficult samples in E.Z.N.A.® Plant RNA Kits (Omega Biotek, Norcross, GA, USA). The samples were reverse transcribed and specific sequences were amplified using the SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... the firefly LUC and Renilla LUC activities were measured by Dual Luciferase Reporter Gene Assay Kit (Yeasen, Shanghai) and Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, USA). Activity is expressed as the ratio of firefly LUC activity to Renilla LUC activity ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from pure cultures using the Omega Mag-Bind Universal Pathogen Kit according to manufacturer’s protocol with a final elution volume of 60µl (Omega Biotek Inc., Norcross, GA). DNA samples were quantified using Qubit fluorometer (ThermoFisher Scientific ...