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Citations for Biotek :
201 - 250 of 268 citations for 3 Thiophen 3 yl benzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Fluorescence (Ex = 335 nm and Em = 450 nm) was measured every minute on a Cytation 3 imaging reader (BioTek, Winooski, VT, USA) for 60 min ...
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bioRxiv - Microbiology 2024Quote: ... Quantification of the extracted DNA was conducted spectrophotometrically with the Take 3 plate in an Eon plate reader/incubator (BioTek, Winooski, VT).
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bioRxiv - Microbiology 2024Quote: ... Fluorescence microscopy images (4X and 40X magnification) were acquired with the Cytation™ 3 Cell Imaging Multi-Mode Reader (BioTek® Instruments). Macrophage-residing HIV-1 in VCCs was analyzed with 40X magnification images ...
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bioRxiv - Bioengineering 2023Quote: ... It was then incubated for 1 hour at room temperature before the absorbance was read at 570 nm using a microplate reader (BioTek, Cytation 3). The manufacturer’s standard protocol was used to calibrate the measurements.
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bioRxiv - Synthetic Biology 2023Quote: ... The plate was shaken at 300 rpm (orbital) for 3 min and BRET biosensor performance was recorded using the plate reader’s luminescence filter (BioTek Synergy H1, v2.04.11) with an integration time of 1 s ...
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bioRxiv - Microbiology 2024Quote: ... to initiate reactions and then the fluorescence signal (λex= 335 nm and λem= 450 nm) was monitored by Cytation 3 Multi-Mode Reader (BioTek, Winooski, VT) at 25 °C for 1 hour ...
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bioRxiv - Immunology 2024Quote: ... Fluorescence (λex = 380 nm, λem = 460 nm) of the AMC product was monitored by Cytation 3 Multi-Mode Reader (BioTek, Winooski, VT) (54 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... were added to wells in a microplate and incubated for 2 min at 37°C in a microplate reader (Cytation 3 Imaging Reader, BioTek, VT, USA). Then ...
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bioRxiv - Microbiology 2024Quote: ... Quantity and quality of the extracted DNA was determined with the Take 3 plate in an Eon plate reader/incubator (BioTek, Winooski, VT) (Culliney and Schmalenberger ...
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bioRxiv - Biochemistry 2020Quote: ... 30 μL of a 10 nM solution of FphF in PBS/0.01%TritonX-100 was added and fluorescence (λex = 365 nm and λem = 455 nm) was read at 37°C in 1 min intervals on a Cytation 3 imaging reader (BioTek, Winooski, VT, USA) for 60 min ...
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bioRxiv - Cell Biology 2022Quote: ... The reduction of resazurin to resorufin was measured fluorometrically at 540 nm excitation and 590 nm emission in a Cytation 3 microplate reader (BioTek Instruments Inc., USA). The results were expressed as % of untreated cells ...
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bioRxiv - Microbiology 2020Quote: ... Em = 450 nm) over a period of 30-60 minutes at 37°C on a Cytation-3 imaging reader (BioTek, Winooski, VT, USA). Concentrations of hydrolyzed products were determined using a standard concentration curve (unconjugated 4MU) ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were incubated at 37°C and fluorescence was measured (Ex = 410 nm, Em = 450 nm) over a period of 60 minutes on a Cytation 3 imaging reader (BioTek, Winooski, VT, USA). Reaction rates were determined by linear regression analysis of the initial linear phase of hydrolysis ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Relative dopaminergic cell viability was assessed by counting MAP2- and TH-positive neurons in a blinded manner using images taken with an automated Cytation 3 Cell Imaging Reader (BioTek Instruments, Winooski, VT) equipped with a 4X objective ...
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bioRxiv - Microbiology 2023Quote: ... and inhibitor simultaneously Cleavage was measured using fluorescence (EX 380 nm/EM 460 nm) with Cytation 3 imaging reader (BioTek, Winooski, VT, USA) for 60 minutes and slope was used to calculate the inhibition curve ...
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bioRxiv - Cancer Biology 2023Quote: ... incubated for 4 h and fluorescence was read at 550 nm/590 nm on a Cytation 3 Imaging Reader (BioTek, Winooski, Vermont, USA).
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bioRxiv - Synthetic Biology 2023Quote: ... 80 µL ONE-Glo EX Reagent was then added and the plate was orbitally shaken at 300 rpm for 3 min in a microplate reader (BioTek Synergy H1, v2.04.11). Firefly luminescence was collected using the microplate reader’s luminescence fiber ...
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bioRxiv - Cancer Biology 2021Quote: ... was added to each well for 3 h and the absorbance at 450 nm was measured on an Epoch™ Microplate Spectrophotometer (Biotek, Winooski, VT, USA). Cytotoxicity data were expressed as mean ± standard derivation (SD ...
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bioRxiv - Cell Biology 2022Quote: ... 25 μL of xanthine 1x and the absorbance was read at 450 nm in a BioTek Cytation 3™ multiplate reader (BioTek Instruments Inc., USA). Results were expressed as % of the pool of controls.
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bioRxiv - Cell Biology 2024Quote: ... 100 µL of Tris (pH 10) was added and optical density was measured at 540 nm in Biotek Cytation 3 reader (Biotek Instruments, Winooski, VT, USA) and analyzed using the Wave Desktop 2.6.1 software (RRID:SCR_014526).
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bioRxiv - Bioengineering 2023Quote: ... We affixed the balloons to a glass microscope slide using tape and acquired images at 4x magnification with a Cytation 3 Image reader (BioTek Instruments Inc, Winooski, VT). All images were exported using a 16-bit resolution (65536 grey levels ...
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bioRxiv - Bioengineering 2020Quote: ... 5% CO2 using an automated digital microscope with a 10× objective with a gas controller (Cytation 3 microplate reader, BioTek Instruments Inc., Winooski, VT, USA). Images were collected every 10 minutes for 24h ...
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bioRxiv - Cancer Biology 2020Quote: ... was added and intensities were measured for each well using a Cytation 3 Multi Modal Reader with Gen5 software (BioTek Instruments, Inc., Winooski, VT, USA). Along with cell viability ...
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bioRxiv - Biochemistry 2024Quote: The pyrene–actin based bulk–polymerization experiments in the absence and presence of VCA– activated Arp2/3 complex were performed at a Synergy 4™ microplate reader (BioTek Instruments, Winooski, VT, USA) using the built–in filter set (Excitation ...
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bioRxiv - Cell Biology 2023Quote: ... the fluorescence was measured at 340 nm excitation wavelength and 480 nm emission wavelength in a Cytation™ 3 microplate reader (BioTek Instruments Inc., Winooski, VT, USA).
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bioRxiv - Cell Biology 2023Quote: ... The fluorescent signal was measured at 548 nm excitation wavelength and 574 nm emission wavelength in a Cytation™ 3 microplate reader (BioTek Instruments Inc., Winooski, VT, USA). As a positive control ...
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bioRxiv - Plant Biology 2020Quote: ... Nucleic acid concentration was measured using a microplate reader Synergy HT (Biotek, Germany), with the software Gen5™ (Biotek ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... with the Nucleic Acid Quantification workflow on a Synergy HT plate reader (BioTek) with Gen5 software.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The nucleic acid concentration was determined using a microplate reader (Synergy H1; BioTek), and the quality of the RNA was assessed using Agilent 2100 Bioanalyzer system (Agilent Technologies) ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... and yields were quantified the Nucleic Acid Quantification workflow on a Synergy HT plate reader (BioTek) with Gen5 software ...
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bioRxiv - Plant Biology 2020Quote: ... The total amino acid content was then measured in a Synergy HT plate reader (BioTek, Germany) at a wavelength of 550 nm ...
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bioRxiv - Microbiology 2020Quote: ... and RNA concentration was measured by nucleic acid quantification in Epoch Microplate Spectrophotometer (BioTek, Winooski, VT, USA). Transfer RNAs and 5S RNA were then removed using the MEGAclear Kit Purification for Large Scale Transcription Reactions (AMBION ...
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bioRxiv - Neuroscience 2024Quote: ... The extinction of the bicinchoninic acid (BCA) was measured at 562 nm utilizing the µQuant spectrometer (BioTek). A standard curve was set up using albumin to determine the protein concentration of each sample.
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bioRxiv - Cancer Biology 2021Quote: ... Dimerization of Homovanilic acid was recorded every 30 seconds for 20 minutes with a BioTek Synergy H1 platereader (BioTek) spectrophotometer with an Ex = 320 nm and Em = 420 nm.
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bioRxiv - Immunology 2020Quote: ... After 5 to 10 min the reaction was stopped with 100 µL 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek). A standard curve was generated using rec 2-12C antibody (Absolute Antibody) ...
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bioRxiv - Immunology 2021Quote: ... Reactions were quenched with 1 N hydrochloric acid and absorbance was measured at 450 nm using a spectrophotometer (Biotek).
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bioRxiv - Microbiology 2023Quote: ... and protein concentrations determined using a Bicinchoninic Acid Assay (Pierce BCA Protein Assay, ThermoScientific) on an Eon spectrophotometer (BioTek). Total protein lysates were separated via SDS-PAGE and normalized across the isolates using Stain-FreeTM (BioRad ...
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bioRxiv - Cell Biology 2024Quote: ... Brown adipocytes were incubated in phenol red-free DMEM containing 0.5 mM glucose and 1% fatty acid-free BSA and imaged by a Lionheart FX Automated Microscope (BioTek) with a 10× objective in an environmentally controlled chamber at 37 °C and 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... TMB substrate was added and incubated for 2-5 min and reaction was stopped with 50µl of 1M sulphuric acid and absorbance was measured at 450 and 630nm (reference wavelength) on the Cytation3 Imaging Reader (Biotek). The MN titre was defined as the final dilution of serum that caused ≥50% reduction in NP expression.
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bioRxiv - Immunology 2020Quote: ... substrate the reaction was stopped with 1M sulfuric acid and absorbance was measured at 450 nm and 570 nm (reference wavelength) on the Cytation3 Imaging Reader (Biotek). The MN titers were expressed as half maximal inhibitory dilution (50% Inhibitory titer is ...
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bioRxiv - Microbiology 2021Quote: ... The reaction was stopped by adding 50µL of 3M hydrochloric acid and then absorbance (at 490nm) was immediately determined using Synergy H1 hybrid multimode microplate reader (BioTek). PRISM 7.0 was used to determine the effective concentration of each virus that would yield detectable NA activity ...
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bioRxiv - Microbiology 2022Quote: ... and sulfuric acid and quantified at 450 nm using a BioTek Synergy H1 plate reader (BioTek Instruments Inc., Winoski, VT). Values were analyzed in Prism 9.0 (GraphPad ...
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bioRxiv - Microbiology 2022Quote: ... Wells were washed twice with PBS and stained biofilms were resuspended in 7% acetic acid (v/v) and absorbance was read at 595 nm in a Synergy H1 hybrid multimode reader (BioTek).
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bioRxiv - Molecular Biology 2022Quote: ... stopping the reaction with 2M sulfuric acid and by measuring the absorbance at 450nm with a microplate spectrophotometer (Epoch BioTek).
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bioRxiv - Microbiology 2020Quote: ... the reaction was stopped with 50 μls of 3M hydrochloric acid and absorbance was measured at 490 nm (OD490) using a Synergy 4 (BioTek) plate reader ...
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bioRxiv - Microbiology 2021Quote: ... stopped using 3M hydrochloric acid (HCL) solution and plates were read at 490nm wavelength using ELISA plate reader (BioTek Instruments). The endpoint antibody titer was determined by using a cut-off value which is three-times the absorbance of first dilution of mock (uninfected ...
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bioRxiv - Immunology 2020Quote: ... After 5 to 10 min the reaction was stopped with 100 µl 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek). The cut off value was defined as the average of all blank wells plus three times the standard deviation of the blank wells.
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bioRxiv - Immunology 2020Quote: ... After 5 to 10 min the reaction was stopped with 100 µl 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek). The cut off value was defined as the average of all blank wells plus three times the standard deviation of the blank wells.
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bioRxiv - Microbiology 2022Quote: ... The absorbance was quantified at 570 nm using 30% acetic acid in water as the blank by a plate reader (Synergy H1 from BioTek).
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bioRxiv - Immunology 2024Quote: ... Reaction was stopped using 0.5 M/1N sulfuric acid and absorbance measured at 450 nm with subtracting reference at 620 nm using ELISA plate reader (Epoc, BioTek). For K18-hACE2 mice challenged with D614G for unspecific binding ...