Labshake search
Citations for Biotek :
2001 - 2050 of 2258 citations for Prolactin PRL ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Pangenomic Landscapes Shape Performances of a Synthetic Genetic Circuit Across Stutzerimonas SpeciesbioRxiv - Synthetic Biology 2024Quote: ... and mKate (Ex 585/ Em 615, gain 125) fluorescence was measured continuously using a Synergy H1 plate reader (Agilent Biotek, Serial Number 21031715) with continuous linear shaking (1096 cpm ...
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bioRxiv - Physiology 2024Quote: ... Purified total RNA samples were subsequently aliquoted onto a Take3 micro-volume plate and quantified on a Synergy Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA). To determine AedaeItp and AedaeItp-l transcript levels ...
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bioRxiv - Microbiology 2023Quote: ... All endpoint assays and growth curves were carried out at 30°C and 548 rpm using a Synergy HTX plate reader (BioTek, Winooski, VT, USA) with OD600 readings measured every 30 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... Absorbance was measured directly after mixture and then every 10 minutes to ensure values were within standard curve range using a plate reader (SynergyTM HTX, Biotek, Winooski, VT, USA) at 492 nm ...
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bioRxiv - Microbiology 2023Quote: ... incubated for 10 minutes and then fluorescent measurement was applied (Excitation at 550 nm, emission 650 nm) at Biotek Synergy H1 plate reader (Biotek, Winooski, VT, USA).
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bioRxiv - Microbiology 2023Quote: ... The 96-well plates were incubated and growth was recorded using a Synergy H1 Hybrid Multi-Mode Reader (BioTek Instruments, Inc., Vermont, USA) for 30h at 37°C and 5% CO2.
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bioRxiv - Bioengineering 2023Quote: ... The luminescence signal which represents the amount of ATP in viable cells was measured with a microtiter well plate reader (BioTek Cytation 5, Agilent).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 100 μl EGFR-mTurq vesicles were transferred to 96-well plates and full fluorescence emission spectra were collected with a H4 Synergy Hybrid Microplate Reader (BioTek Instruments, Winooski, VT). The samples were excited at 430 nm with a 9 nm bandwidth and the emitted fluorescence was collected from 450 – 620 nm with a 9 nm bandwidth with 5 nm steps ...
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bioRxiv - Synthetic Biology 2023Quote: ... The concentration of GvpU was then measured using absorbance at 280 nm (OD280) on a Take3 plate (Agilent BioTek Synergy H4 Hybrid, Agilent) and adjusted to a final concentration of 1 mM ...
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bioRxiv - Cancer Biology 2023Quote: ... Extracted RNA was quantified and its purity (absorbance at 260/280 nm) was verified using a spectrophotometric plate reader (Synergy HTX multi-mode reader, Biotek Instruments, Winooski, VT). RNA was reverse transcribed into cDNA using the cDNA Superscript Mix (Quanta Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... Absorbance was measured directly after mixture and then every 10 minutes to ensure values were within standard curve range using a plate reader (SynergyTM HTX, Biotek, Winooski, VT, USA) at 492 nm ...
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bioRxiv - Immunology 2023Quote: ... the plate was washed 3x with Wash Buffer (1x PBS and 0.05% (v/v) Tween-20) using a magnetic plate washer (Biotek 405TS, Biotek Instruments GmbH). To detect bound IgG RSV antibodies ...
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bioRxiv - Biochemistry 2023Quote: ... plates were shaking for 30 min and the absorbance was measured at 570 nm in a multiwell plate reader Synergy H1 Hybrid Reader (Biotek Instruments Inc., USA).
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bioRxiv - Biophysics 2022Quote: ... The amount of inorganic phosphate (Pi) at each time point was determined by taking the absorbance at 620 nm using a plate reader (BioTek Synergy Neo 2) and comparing to a standard Pi concentration plot.
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bioRxiv - Microbiology 2023Quote: ... were statically incubated at 30 °C for 48h and biofilm measurements were done as previously reported (41) using a Cytation5 plate reader (BioTek, Winooski, VT, USA). The experiment was replicated three times in total with 36 technical replicates each ...
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bioRxiv - Cell Biology 2023Quote: ... The NBD fluorescence spectra were recorded at 25°C with excitation at 470 nm and an emission range of 520 – 600 nm using a Cytation five imaging plate reader (Biotek Instruments, Winooski, VT). Lipid blanks were prepared at the highest and lowest pH ...
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bioRxiv - Biochemistry 2023Quote: ... Phosphorylation reaction progress was monitored by measuring fluorescence at excitation 530 nm and emission 590 nm every 2 minutes at 37 °C on a plate reader (BioTek Synergy Neo 2). The fluorescence units (RFU ...
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bioRxiv - Synthetic Biology 2023Quote: ... 80 µL ONE-Glo EX Reagent was then added and the plate was orbitally shaken at 300 rpm for 3 min in a microplate reader (BioTek Synergy H1, v2.04.11). Firefly luminescence was collected using the microplate reader’s luminescence fiber ...
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bioRxiv - Microbiology 2023Quote: ... ODs were obtained by measuring absorbance at 600nM in a 96-well plate using Cytation Station 5 (BioTek Agilent Technologies, Santa Clara, CA). 108 colony-forming units (CFUs ...
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bioRxiv - Cancer Biology 2023Quote: ... Extracted RNA was quantified and its purity (absorbance at 260/280 nm) was verified using a spectrophotometric plate reader (Synergy HTX multi-mode reader, Biotek Instruments, Winooski, VT). Library preparation and sequencing were done at the University of Michigan Advanced Genomics Core ...
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bioRxiv - Bioengineering 2023Quote: ... Absorbance was measured at 450 nm at the start of the incubation period and after 15 minutes using a plate reader (SynergyTM HTX, Biotek, Winooski, VT, USA). ALP activity per explant was calculated using standards with known concentrations of p-nitrophenol ...
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bioRxiv - Cell Biology 2023Quote: ... the stained cells were subjected to measurements of the absorbances of 455 nm and 525 nm at 37 °C using a plate reader equipped with kinetic mode (BioTek SynergyH4 Hybrid Reader).
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bioRxiv - Molecular Biology 2023Quote: ... Fluorescence was measured at 10 seconds at Relative Luciferase Units (RLU) using a luminometer microplate reader (Biotek synergy H1 hybrid plate reader with Gen5v. Software, Biotek, Winooski, VT, USA).
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bioRxiv - Microbiology 2023Quote: ... ODs were obtained by measuring absorbance at 600nM in a 96-well plate using Cytation Station 5 (BioTek Agilent Technologies, Santa Clara, CA). The liquid cultures were centrifuged at 3500 rpm for 5 minutes (Sorvall Legend X1R M20 rotor ...
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bioRxiv - Microbiology 2024Quote: ... with ancestral or evolved phages were obtained from sequential OD600 readings using a microtiter plate reader (Epoch Microplate spectrophotometer; BioTek, Winooski, VT, USA). We added bacterial cultures at exponential growth to individual wells of a 96-well microtiter plate and mixed them with phage lysate at an MOI of 1 ...
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bioRxiv - Cell Biology 2024Quote: ... Pyrene fluorescence was measured using time-based scans on a plate reader (Biotek Synergy H4 Hybrid Multi-Mode Microplate Reader with Gen5 software, BioTek Instruments, Winooski, VT) at 25°C with excitation at 365 nm and emission at 407 nm.
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bioRxiv - Synthetic Biology 2022Quote: ... with Cytation 1 (BioTek), or visualized with a blue light or UV transilluminator ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we performed automated growth-assays on each of the 24 revived populations using a well-plate reader in multiple environments (Synergy HT, BIOTEK ® Winooski, VT, USA). Using optical density at 600 nm as the measure of population density ...
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bioRxiv - Bioengineering 2021Quote: ... Methods for aggregation assays for recombinant human insulin were adapted from Webber et al.11 Formulation samples were plated at 150 μL per well in a clear 96-well plate and an absorbance reading was taken at 540 nm (BioTek Synergy H1 microplate reader). The aggregation of insulin leads to light scattering ...
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bioRxiv - Neuroscience 2019Quote: ... Experiments with CSF samples and neurons on 96-well plates were acquired using a Biotek Cytation 3 Imaging Reader (BioTek Instruments, Winooski, VT, USA) equipped with a 20× ...
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Mitochondrial and metabolic remodeling in human skin fibroblasts in response to glucose availabilitybioRxiv - Cell Biology 2021Quote: ... and the kinetics of the fluorescence signal was recorded in a BioTek® Cytation3 UV–vis multi-well plate imaging reader (BioTek, Winooski, Vermont, USA) for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... to ∼5 × 106 CFU/mL and growth determined after 16 h incubation by removing 200 µL samples which were transferred to a polystyrene 96-well plate and OD570 measured using a BioTek ELx800 (BioTek Instruments, Winooski, VT, USA) plate reader.
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bioRxiv - Microbiology 2020Quote: ... Frozen −80°C stocks of each pathogen were thawed and grown to early/mid exponential phase using a Cytation3 plate reader (BioTek Instruments Inc., Winooski, VT) and approximately 2×105 CFU/mL of pathogen was inoculated into 180 μL of sterile Luria Bertani (LB ...
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bioRxiv - Microbiology 2020Quote: ... which were incubated during 22 h at 37 °C with shaking (250 rpm) in a plate reader (Synergy HTX Multi-Mode Reader, BioTek Instruments, Inc, VT, USA). Optical densities (OD ...
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bioRxiv - Physiology 2022Quote: ... All assays were run in duplicate under control conditions or in the presence of 5 mM DTT in a 96-well plate and changes in absorbance were tracked using a microplate reader (Synergy 2, BioTek/Agilent, Santa Clara, CA). This concentration was selected based on similar experiments in a previous study [15] ...
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bioRxiv - Neuroscience 2022Quote: ... The serum was diluted 1:100 for LBP detection and the optical density (OD) of the plate was read at 450 nanometers (nm) by an Eon Microplate Spectrophotometer (BioTek Instruments Inc., Winooski VT). Data was calculated from a serial dilution curve using Gen5 Data Analysis Software and samples with a coefficient of variation above 15% were eliminated from the analysis.
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bioRxiv - Biochemistry 2021Quote: ... The reaction was stopped with 2M of sulfuric acid and absorbance measured at 450 nm in a Cytation5® plate reader (BioTek, Winooski, VT, USA). The performance of blank ...
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bioRxiv - Microbiology 2022Quote: ... The plate was then cooled to room temperature before the optical density at 562 nm (OD562) was measured in a BioTek Synergy H1 plate reader (BioTek Instruments, Inc, Winooski, VT) after a 5 second orbital shake ...
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bioRxiv - Synthetic Biology 2022Quote: ... 200 mL of a cell suspension at an optical density measured at 600 nm (OD600) of 0.05 were incubated with orbital shaking in a Synergy H1 microtiter plate reader (BioTek Instruments Inc., Winooski, VT, USA) or in an ELx808 microtiter plate reader (BioTek Instruments Inc.) ...
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bioRxiv - Bioengineering 2024Quote: ... DMMB solution was added (200 µL/well) and absorbance was measured at 540 nm and 590 nm using a plate reader (BioTek Cytation 5, Agilent Technologies). For all samples ...
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bioRxiv - Bioengineering 2024Quote: ... Fluorescence was quantified using an excitation of 530/15 nm and emission of 590/15 nm on a fluorescent plate reader (BioTek Cytation 5, Agilent Technologies).
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bioRxiv - Immunology 2023Quote: ... The decreased calcein fluorescence in target cells due to cell lysis was measured at 485 nm excitation wavelength and 528 nm emission wavelength in the bottom reading mode using a Synergy HTX multi-mode plate reader (BioTek, Santa Clara, California, USA). The fluorescence for the experimental condition was adjusted by the parameter γ according to the live target cell control fluorescence ...
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bioRxiv - Plant Biology 2023Quote: ... Absorbance measurements at 649 nm (A649) and 665 nm (A665) were recorded in each well using a plate reader (Biotek Synergy H1; Biotek Instruments, Winooski, VT USA), with triplicates averaged and corrected by the mean of the blank absorbance value ...
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bioRxiv - Microbiology 2023Quote: ... We performed PCA oxidation assays either by tracking the fluorescence of reduced PCA over time in a plate reader (BioTek Synergy 4 or HTX) housed in a Coy-brand anoxic chamber or by tracking the current generated by a culture when provided PCA and an electrode poised to continuously reduce any PCA that the cells oxidized ...
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bioRxiv - Cell Biology 2023Quote: ... the fluorescence of antibodies was measured at 633 nm excitation/665 nm emission using a plate reader (Biotek Synergy H4 or Biotek Synergy Neo2) in technical triplicate as we previously described41 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We then grew all the 96 revived populations in each of the five environments and measured their optical density at 600 nm every 20 minutes using an automated well-plate reader (Synergy HT, BIOTEK® Winooski, VT, USA). The physical conditions during growth measurement were identical to those during the evolution experiment ...
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bioRxiv - Genomics 2020Quote: ... SQ RNA/DNA/Protein Extraction Kit (Omega Biotek Inc.). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... an E.Z.N.A soil DNA kit (Omega Biotek,GA, USA) was used to extract total genomic DNA ...
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bioRxiv - Immunology 2024Quote: ... HP Total RNA Kit (Omega BioTek, CAT#R6812-02). RNA was reverse transcribed to cDNA using the ABI Reverse Transcription Reagents system ...
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bioRxiv - Physiology 2019Quote: ... We calculated T concentration by comparing sample absorbance with the absorbance of the assay’s standard curve (Gen5 curve-fitting software, Biotek EPOCH plate reader, Winooski, VT, USA). Intra-assay CV was 4.44% and inter-plate CV was 5.94%.