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Citations for Biotek :
151 - 200 of 301 citations for QuantiFluo Protein Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2019Quote: ... Assay plates were incubated for 24 h (30°C, 160 rpm) in a Cytation3 cell imaging multimode reader (BioTek Instruments, Winooski, VT, USA). At 24 h ...
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bioRxiv - Genetics 2020Quote: ... Luciferase activity was determined by analyzing 50 μl of each cell extract in a luciferase assay system according to the standard protocol for the Synergy 2 Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA).
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bioRxiv - Microbiology 2023Quote: ... All endpoint assays and growth curves were carried out at 30°C and 548 rpm using a Synergy HTX plate reader (BioTek, Winooski, VT, USA) with OD600 readings measured every 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µl of the medium of the upper compartment was pipetted in a black 96-wells assay plate and fluorescence was measured with a plate reader (SynergyTM HTX, Biotek, Winooski, VT, USA) at excitation 530/25 nm ...
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bioRxiv - Bioengineering 2023Quote: ... 100 µl of the medium was pipetted in a black 96-wells assay plate and fluorescence was measured with a plate reader (SynergyTM HTX, Biotek, Winooski, VT, USA) at excitation 530/25 nm ...
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bioRxiv - Plant Biology 2022Quote: ... SP Plant DNA Kit (Omega BioTek, Norcross, GA) (Pyne et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA Kit 1 (Omega BioTek, Norcross, GA). In conditions where we wanted to observe the effects in myotubes ...
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bioRxiv - Biochemistry 2020Quote: ... Low-affinity complexes and unbound proteins were removed in consecutive washing steps with PBS-T 0.05% (EL406 washer, BioTek). Beads were incubated for 10 minutes with 0.4% paraformaldehyde ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we performed automated growth-assays on each of the 24 revived populations using a well-plate reader in multiple environments (Synergy HT, BIOTEK ® Winooski, VT, USA). Using optical density at 600 nm as the measure of population density ...
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bioRxiv - Microbiology 2023Quote: ... We performed PCA oxidation assays either by tracking the fluorescence of reduced PCA over time in a plate reader (BioTek Synergy 4 or HTX) housed in a Coy-brand anoxic chamber or by tracking the current generated by a culture when provided PCA and an electrode poised to continuously reduce any PCA that the cells oxidized ...
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bioRxiv - Immunology 2024Quote: ... HP Total RNA Kit (Omega BioTek, CAT#R6812-02). RNA was reverse transcribed to cDNA using the ABI Reverse Transcription Reagents system ...
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bioRxiv - Microbiology 2024Quote: ... an E.Z.N.A soil DNA kit (Omega Biotek,GA, USA) was used to extract total genomic DNA ...
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bioRxiv - Plant Biology 2019Quote: ... and the fluorescence intensity of Venus-tagged protein was measured on a Synergy H4 Hybrid reader (Biotek, Winooskit, VT, USA) at excitation and emission wavelengths of 485 nm and 528 nm ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined by taking OD at 280 nm in Nano drop (Synergy H1 Hybrid Multi-Mode Reader Biotek) and mass extinction co-efficient of the proteins.
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bioRxiv - Microbiology 2020Quote: The TreS activity domain of TreS-Mak protein was monitored following the conversion of trehalose into maltose and glucose using Epoch spectrophotometer (Biotek). 15µL of reaction sample were incubated 30 min at 58°C with 30 µL of 100 mM sodium citrate pH 4.6 containing whether 0.4 U of amyloglucosidase from Aspergillus niger (Megazyme ...
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bioRxiv - Neuroscience 2020Quote: ... All assays were allowed to incubate at 37°C for 30 min before protein concentration was read in at 562 nm (SynergyMix, Biotek).
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bioRxiv - Cancer Biology 2022Quote: ... Cell and tissue debris was removed by centrifugation at 16,000x g for 5 minutes at room temperature and protein concentration was measured using A280 values determined by Cytation 5 (BioTek). Lysates were normalized in Buffer A with 1% SDS and prepared with 6X Laemmli sample buffer containing 100 mM 2-mercaptoethanol (Sigma M6250) ...
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bioRxiv - Microbiology 2023Quote: ... Fluorescence of the Amcyan and Turborfp proteins produced by the spot colonies from each well was measured using the Synergy H1 plate reader (BioTek). We used the constitutive fluorescence of Amcyan to normalize TurboRFP production by reporter expression ...
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bioRxiv - Microbiology 2023Quote: ... The green fluorescent protein (GFP)-positive cells were then counted using Cytation 5 with Gen5 v3.03 software (BioTek, Winooski, VT). Dose-response curves were plotted and EC50 values determined using Prism 9 (GraphPad ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell debris was removed by centrifugation at 16,000x g for 5 minutes at room temperature and protein concentration was measured using A280 values determined by Cytation 5 (BioTek). Lysates were normalized in Buffer A with 1% SDS and prepared with 6X Laemmli sample buffer containing 100 mM 2-mercaptoethanol (Sigma M6250) ...
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bioRxiv - Microbiology 2024Quote: ... Samples were incubated at 95°C for 5 minutes and proteins were quantified using tryptophan fluorescence in a Synergy H1M microplate reader (BioTek) with an excitation wavelength of 280 nm and an emission wavelength of 360 nm ...
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bioRxiv - Physiology 2019Quote: ... We calculated T concentration by comparing sample absorbance with the absorbance of the assay’s standard curve (Gen5 curve-fitting software, Biotek EPOCH plate reader, Winooski, VT, USA). Intra-assay CV was 4.44% and inter-plate CV was 5.94%.
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... We calculated T concentration by comparing sample absorbance with the absorbance of the assay’s standard curve (Gen5 curve-fitting software, Biotek EPOCH plate reader, Winooski, VT, USA). Samples from 3 females and 2 males initially showed greater than 80% maximum binding ...
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bioRxiv - Biochemistry 2023Quote: ... The xanthine/xanthine oxidase method based on the production of O2- anions was used to assay T-SOD activity by ELISA (Biotek, Vermont, America, λ= 450 nm). The liveness of T-SOD is shown as units per milligram of protein (U/mg protein).
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bioRxiv - Bioengineering 2019Quote: ... emission at 517 nm) of the membrane and cytoplasmic proteins was determined using fluorescence spectrophotometer (Synergy H4; BioTek, Winooski, VT, USA).
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bioRxiv - Neuroscience 2021Quote: ... Plasma osmolality was analyzed by vapor pressure osmometry (VAPRO 5520) and volemia was indirectly inferred by the plasma protein concentration which was measured in an absorbance microplate reader (BioTek EL800), according to the protocol proposed by Lowry et al ...
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bioRxiv - Synthetic Biology 2022Quote: The fluorescence intensity of the synthesized fluorescent proteins was measured by the multi-well plate fluorometer (Synergy HTX, BioTek, Winooski, VT). 5 µL of the cell-free synthesized fluorescent protein and 45 µL of Milli-Q water were mixed in a 96-well half-area black plate (Corning Incorporated ...
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bioRxiv - Biophysics 2023Quote: Assessment of the extent of protein oligomerization was based on monitoring absorbance at 550 nm using BioTek Plate Reader (Agilent BioTek NEO2) to reflect turbidity changes induced by protein oligomer formation ...
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bioRxiv - Neuroscience 2024Quote: ... with Coomassie Blue-protein interaction assessed by absorbance at 595 nm using a microplate spectrophotometer (BioTek, Vermont, USA, VT 05404-0998). Sample concentrations were then adjusted to 1000 or 2000 µg/ml for Western blot analysis based on total protein yield ...
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bioRxiv - Microbiology 2024Quote: ... fumarate) or flurometric (citrate, acetyl-CoA) assays (cat. no. KA1674, ab102516, KA3791 and MAK039, respectively) and a microplate reader (BioTek Synergy Neo2, Agilent, Santa Clara, CA) according to manufacturer’s instructions (Abnova ...
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bioRxiv - Plant Biology 2019Quote: ... Plant RNA kit with DNA digestion on column (Biotek, China) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmids were isolated using a DNA miniprep kit (Omega BioTek) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... or the E.Z.N.A.® HP Total RNA Kit (Omega BioTek). RNA was reverse transcribed to cDNA using the ABI Reverse Transcription Reagents system ...
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bioRxiv - Immunology 2023Quote: ... and E.Z.N.A.® HP Total RNA kit (Omega Biotek, U.S.). RNA samples were sequenced using the Illumina HiSeq platform to generate 50-bp paired-end reads ...
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bioRxiv - Developmental Biology 2022Quote: ... and using Total RNA Kit I (Omega Biotek, R6834-02) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... Red fluorescent protein (RFP) readings (excitation 580 nm, emission 610 nm) were taken using a BioTek Synergy H1m plate reader (BioTek, Winooski, VT) after 18 hours of incubation at 37 °C with 200 rpm shaking.
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bioRxiv - Microbiology 2022Quote: ... The absorbance of each well with reaction mixture and protein (but without formaldehyde) was measured at 340 nm with a BioTek Synergy H1 plate reader (BioTek, Winooski, VT) in a Corning CoStar 48 well plate (Corning ...
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bioRxiv - Neuroscience 2022Quote: ... which used bovine serum albumin standards to create a best fit linear regression line based on protein concentrations using the 562nm absorbance mode of a microplate reader and its Gen5 Data Analysis program (Biotek, Winooski, VT). Equal amounts of protein were resolved in SDS-polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... which used bovine serum albumin standards to create a best fit linear regression line based on protein concentrations using the 562nm absorbance mode of a microplate reader and its Gen5 Data Analysis program (Biotek, Winooski, VT). Equal amounts of protein were resolved in SDS-polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Synthetic Biology 2023Quote: The relative fluorescence units (RFU) of the synthesized fluorescent proteins were measured by the multi-well plate fluorometer (Synergy HTX, BioTek, Winooski, VT). 5 μL of the cell-free synthesized fluorescent protein and 45 μL of Milli-Q water were mixed in a 96-well half-area black plate (Corning Incorporated ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 mM Tris was used to extract protein-bound dye and optical density was measured with a BioTek Synergy HT plate reader (SN 269140, BioTek Instruments Inc.) at 490 and 540 nm ...
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bioRxiv - Immunology 2020Quote: ... Plasmids were purified using the EZNA plasmid maxi kit (Omega BioTek), according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... and DNA purification and gel extraction kits (Omega Biotek, GA, USA) were used to amplify DNA fragments and purify the PCR products ...
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bioRxiv - Microbiology 2023Quote: ... followed by extraction using E.Z.N.A Viral RNA Kit (R6874, Omega Biotek). Then ...
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bioRxiv - Plant Biology 2023Quote: ... The RNA-prep pure PLANT Kit (DP432, Tiangen Biotek, Beijing, China) was used to isolate total RNA from the rice grains ...
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bioRxiv - Synthetic Biology 2024Quote: ... Amplicons are then purified using EZNA Cycle Pure kits (Omega BioTek). Isothermal assembly followed Gibson Assembly protocols (NEB) ...
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bioRxiv - Plant Biology 2024Quote: ... RNA extraction was performed using E.Z.N.A Total RNA kit (Omega BioTek) following manufacturer directions ...
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bioRxiv - Immunology 2022Quote: ... Recombinant protein was checked for protein concentration by OD 260/280/320 using a Take3 micro-volume plate with a SynergyMX plate reader (BioTek, Winooski, VT, USA). NEU3 is 51 kDa ...
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bioRxiv - Immunology 2020Quote: ... Buffer-exchanged preparations were then checked for protein concentration by OD 260/280/320 using a Take3 micro-volume plate with a SynergyMX plate reader (BioTek, Winooski, VT, USA) as previously described (54) ...
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bioRxiv - Synthetic Biology 2021Quote: ... The amplicon was then purified using a Cycle Pure Kit (Omega Biotek). The vector backbone fragment was amplified from pET28a-M-CaM-cTdTS plasmid DNA constructed above using PfuUltra II Hotstart PCR Master Mix using DNA primers P29 – P36 listed in Table 1 ...