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Citations for Biotek :
151 - 200 of 375 citations for Mouse Acrosomal Protein SP 10 ACRV1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Low-affinity complexes and unbound proteins were removed in consecutive washing steps with PBS-T 0.05% (EL406 washer, BioTek). Beads were incubated for 10 minutes with 0.4% paraformaldehyde ...
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bioRxiv - Biochemistry 2020Quote: ... Readings were taken once every 10 seconds and analyzed by the Gen5 Data Analysis Software (BioTek). (3 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the plate was incubated for 10 minutes before FP measurements with a BioTek Synergy 4 (BioTek) at excitation and emission wavelengths of 485 and 528 nm ...
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bioRxiv - Immunology 2024Quote: ... HP Total RNA Kit (Omega BioTek, CAT#R6812-02). RNA was reverse transcribed to cDNA using the ABI Reverse Transcription Reagents system ...
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bioRxiv - Microbiology 2024Quote: ... an E.Z.N.A soil DNA kit (Omega Biotek,GA, USA) was used to extract total genomic DNA ...
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bioRxiv - Plant Biology 2019Quote: ... and the fluorescence intensity of Venus-tagged protein was measured on a Synergy H4 Hybrid reader (Biotek, Winooskit, VT, USA) at excitation and emission wavelengths of 485 nm and 528 nm ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined by taking OD at 280 nm in Nano drop (Synergy H1 Hybrid Multi-Mode Reader Biotek) and mass extinction co-efficient of the proteins.
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bioRxiv - Microbiology 2020Quote: The TreS activity domain of TreS-Mak protein was monitored following the conversion of trehalose into maltose and glucose using Epoch spectrophotometer (Biotek). 15µL of reaction sample were incubated 30 min at 58°C with 30 µL of 100 mM sodium citrate pH 4.6 containing whether 0.4 U of amyloglucosidase from Aspergillus niger (Megazyme ...
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bioRxiv - Neuroscience 2020Quote: ... All assays were allowed to incubate at 37°C for 30 min before protein concentration was read in at 562 nm (SynergyMix, Biotek).
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bioRxiv - Cancer Biology 2022Quote: ... Cell and tissue debris was removed by centrifugation at 16,000x g for 5 minutes at room temperature and protein concentration was measured using A280 values determined by Cytation 5 (BioTek). Lysates were normalized in Buffer A with 1% SDS and prepared with 6X Laemmli sample buffer containing 100 mM 2-mercaptoethanol (Sigma M6250) ...
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bioRxiv - Microbiology 2023Quote: ... Fluorescence of the Amcyan and Turborfp proteins produced by the spot colonies from each well was measured using the Synergy H1 plate reader (BioTek). We used the constitutive fluorescence of Amcyan to normalize TurboRFP production by reporter expression ...
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bioRxiv - Microbiology 2023Quote: ... The green fluorescent protein (GFP)-positive cells were then counted using Cytation 5 with Gen5 v3.03 software (BioTek, Winooski, VT). Dose-response curves were plotted and EC50 values determined using Prism 9 (GraphPad ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell debris was removed by centrifugation at 16,000x g for 5 minutes at room temperature and protein concentration was measured using A280 values determined by Cytation 5 (BioTek). Lysates were normalized in Buffer A with 1% SDS and prepared with 6X Laemmli sample buffer containing 100 mM 2-mercaptoethanol (Sigma M6250) ...
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bioRxiv - Molecular Biology 2023Quote: ... Fluorescence was measured at 10 seconds at Relative Luciferase Units (RLU) using a luminometer microplate reader (Biotek synergy H1 hybrid plate reader with Gen5v ...
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bioRxiv - Cancer Biology 2024Quote: ... Luminescence was read every 10 min for 1h using Cytation 5 microplate reader and Gen5 software (BioTek). Relative NAD(H ...
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bioRxiv - Microbiology 2024Quote: ... 450 nm) from 10 min to 45 min was obtained by the plate reader (Synergy HTX, BioTek) and used as the NA activity measured by MUNANA assay ...
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bioRxiv - Bioengineering 2019Quote: ... emission at 517 nm) of the membrane and cytoplasmic proteins was determined using fluorescence spectrophotometer (Synergy H4; BioTek, Winooski, VT, USA).
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bioRxiv - Neuroscience 2021Quote: ... Plasma osmolality was analyzed by vapor pressure osmometry (VAPRO 5520) and volemia was indirectly inferred by the plasma protein concentration which was measured in an absorbance microplate reader (BioTek EL800), according to the protocol proposed by Lowry et al ...
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bioRxiv - Synthetic Biology 2022Quote: The fluorescence intensity of the synthesized fluorescent proteins was measured by the multi-well plate fluorometer (Synergy HTX, BioTek, Winooski, VT). 5 µL of the cell-free synthesized fluorescent protein and 45 µL of Milli-Q water were mixed in a 96-well half-area black plate (Corning Incorporated ...
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bioRxiv - Biophysics 2023Quote: Assessment of the extent of protein oligomerization was based on monitoring absorbance at 550 nm using BioTek Plate Reader (Agilent BioTek NEO2) to reflect turbidity changes induced by protein oligomer formation ...
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bioRxiv - Plant Biology 2019Quote: ... Plant RNA kit with DNA digestion on column (Biotek, China) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmids were isolated using a DNA miniprep kit (Omega BioTek) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... or the E.Z.N.A.® HP Total RNA Kit (Omega BioTek). RNA was reverse transcribed to cDNA using the ABI Reverse Transcription Reagents system ...
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bioRxiv - Immunology 2023Quote: ... and E.Z.N.A.® HP Total RNA kit (Omega Biotek, U.S.). RNA samples were sequenced using the Illumina HiSeq platform to generate 50-bp paired-end reads ...
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bioRxiv - Developmental Biology 2022Quote: ... and using Total RNA Kit I (Omega Biotek, R6834-02) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... Red fluorescent protein (RFP) readings (excitation 580 nm, emission 610 nm) were taken using a BioTek Synergy H1m plate reader (BioTek, Winooski, VT) after 18 hours of incubation at 37 °C with 200 rpm shaking.
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bioRxiv - Immunology 2019Quote: ... The supernatant was subjected to a BCA protein assay (Pierce Biotechnology, Waltham, MA) and measured on a Synergy H1 Hybrid Multi-Mode Reader (BioTek, Winooski, VT). After normalizing the samples to the lowest sample concentration through the addition of PBS ...
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bioRxiv - Microbiology 2022Quote: ... The absorbance of each well with reaction mixture and protein (but without formaldehyde) was measured at 340 nm with a BioTek Synergy H1 plate reader (BioTek, Winooski, VT) in a Corning CoStar 48 well plate (Corning ...
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bioRxiv - Neuroscience 2022Quote: ... which used bovine serum albumin standards to create a best fit linear regression line based on protein concentrations using the 562nm absorbance mode of a microplate reader and its Gen5 Data Analysis program (Biotek, Winooski, VT). Equal amounts of protein were resolved in SDS-polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Synthetic Biology 2023Quote: The relative fluorescence units (RFU) of the synthesized fluorescent proteins were measured by the multi-well plate fluorometer (Synergy HTX, BioTek, Winooski, VT). 5 μL of the cell-free synthesized fluorescent protein and 45 μL of Milli-Q water were mixed in a 96-well half-area black plate (Corning Incorporated ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 mM Tris was used to extract protein-bound dye and optical density was measured with a BioTek Synergy HT plate reader (SN 269140, BioTek Instruments Inc.) at 490 and 540 nm ...
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bioRxiv - Neuroscience 2024Quote: ... which used bovine serum albumin standards to create a best fit linear regression line based on protein concentrations using the 562nm absorbance mode of a microplate reader and its Gen5 Data Analysis program (Biotek, Winooski, VT). Equal amounts of protein were resolved in SDS-polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... In the linear phase of the reaction (10 – 40 min) turnover rates were calculated using Gen5 software (BioTek) as RFU/min and were normalized by subtracting background hydrolysis rates measured for each substrate in reaction buffer in the absence of protein.
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bioRxiv - Immunology 2022Quote: ... 1:10) were prepared in PBS and the absorbance was measured at 380 nm (Epoch Microplate Spectrophotometer, BioTek) to calculate the amount needed to make the required dose using the bacterial growth standard curve.
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bioRxiv - Cell Biology 2019Quote: ... mixed and the luminescence after 10 minutes of incubation was read in a microplate reader (Synergy H1, Biotek). Cell proliferation rates determined from a standard calibration curve and reported as log2 fold change of cell numbers relative to that at the initial time point prior to treatment.
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bioRxiv - Immunology 2020Quote: ... Plasmids were purified using the EZNA plasmid maxi kit (Omega BioTek), according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... and DNA purification and gel extraction kits (Omega Biotek, GA, USA) were used to amplify DNA fragments and purify the PCR products ...
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bioRxiv - Microbiology 2023Quote: ... followed by extraction using E.Z.N.A Viral RNA Kit (R6874, Omega Biotek). Then ...
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bioRxiv - Plant Biology 2023Quote: ... The RNA-prep pure PLANT Kit (DP432, Tiangen Biotek, Beijing, China) was used to isolate total RNA from the rice grains ...
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bioRxiv - Synthetic Biology 2024Quote: ... Amplicons are then purified using EZNA Cycle Pure kits (Omega BioTek). Isothermal assembly followed Gibson Assembly protocols (NEB) ...
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bioRxiv - Plant Biology 2024Quote: ... RNA extraction was performed using E.Z.N.A Total RNA kit (Omega BioTek) following manufacturer directions ...
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bioRxiv - Immunology 2022Quote: ... Recombinant protein was checked for protein concentration by OD 260/280/320 using a Take3 micro-volume plate with a SynergyMX plate reader (BioTek, Winooski, VT, USA). NEU3 is 51 kDa ...
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bioRxiv - Immunology 2020Quote: ... Buffer-exchanged preparations were then checked for protein concentration by OD 260/280/320 using a Take3 micro-volume plate with a SynergyMX plate reader (BioTek, Winooski, VT, USA) as previously described (54) ...
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bioRxiv - Neuroscience 2023Quote: ... and protein concentrations were determined using the Bradford Assay and an Epoch BioTek Microplate Spectrophotometer (Gen 5 2.01; BioTek Instruments Inc; Winooski, VT). 25 μg of protein from tissue homogenates were combined with specific cytokine antibody-immobilized beads ...
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bioRxiv - Neuroscience 2021Quote: ... λem= 490 nm was measured every 10 min for up to 24 h using a fluorescence plate reader (Biotek). Once the optimal concentrations of approximately 20 μM Aβ42 ...
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bioRxiv - Microbiology 2022Quote: ... The growth was assessed by measuring the OD600 every 10 minutes for 72 hours using a microplate reader (Biotek synergy H1 ...
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bioRxiv - Microbiology 2022Quote: ... The growth was assessed by measuring the OD600 every 10 minutes for 72 hours using a microplate reader (Biotek synergy H1 ...
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bioRxiv - Microbiology 2022Quote: ... The growth was assessed by measuring the OD600 every 10 minutes for 72 hours using a microplate reader (Biotek synergy H1 ...
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bioRxiv - Bioengineering 2023Quote: ... Cell abundance in the multiwell was measured every 10 min by OD600 using a microplate spectrophotometer (Epoch 2, Biotek). Each microplate was incubated at 37°C for 20 h in shaking mode ...
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bioRxiv - Cell Biology 2023Quote: ... 5 mM d-glucose) twice before a 10 minute incubation in a 37°C plate reader (Synergy H4, BioTek), optionally with DMSO or Dyngo4a (30 μM ...