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Citations for Biotek :
1901 - 1950 of 2116 citations for Allopregnanolone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... we performed automated growth-assays on each of the 24 revived populations using a well-plate reader in multiple environments (Synergy HT, BIOTEK ® Winooski, VT, USA). Using optical density at 600 nm as the measure of population density ...
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bioRxiv - Bioengineering 2021Quote: ... Methods for aggregation assays for recombinant human insulin were adapted from Webber et al.11 Formulation samples were plated at 150 μL per well in a clear 96-well plate and an absorbance reading was taken at 540 nm (BioTek Synergy H1 microplate reader). The aggregation of insulin leads to light scattering ...
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bioRxiv - Neuroscience 2019Quote: ... Experiments with CSF samples and neurons on 96-well plates were acquired using a Biotek Cytation 3 Imaging Reader (BioTek Instruments, Winooski, VT, USA) equipped with a 20× ...
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Mitochondrial and metabolic remodeling in human skin fibroblasts in response to glucose availabilitybioRxiv - Cell Biology 2021Quote: ... and the kinetics of the fluorescence signal was recorded in a BioTek® Cytation3 UV–vis multi-well plate imaging reader (BioTek, Winooski, Vermont, USA) for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... to ∼5 × 106 CFU/mL and growth determined after 16 h incubation by removing 200 µL samples which were transferred to a polystyrene 96-well plate and OD570 measured using a BioTek ELx800 (BioTek Instruments, Winooski, VT, USA) plate reader.
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bioRxiv - Microbiology 2020Quote: ... Frozen −80°C stocks of each pathogen were thawed and grown to early/mid exponential phase using a Cytation3 plate reader (BioTek Instruments Inc., Winooski, VT) and approximately 2×105 CFU/mL of pathogen was inoculated into 180 μL of sterile Luria Bertani (LB ...
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bioRxiv - Microbiology 2020Quote: ... which were incubated during 22 h at 37 °C with shaking (250 rpm) in a plate reader (Synergy HTX Multi-Mode Reader, BioTek Instruments, Inc, VT, USA). Optical densities (OD ...
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bioRxiv - Physiology 2022Quote: ... All assays were run in duplicate under control conditions or in the presence of 5 mM DTT in a 96-well plate and changes in absorbance were tracked using a microplate reader (Synergy 2, BioTek/Agilent, Santa Clara, CA). This concentration was selected based on similar experiments in a previous study [15] ...
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bioRxiv - Neuroscience 2022Quote: ... The serum was diluted 1:100 for LBP detection and the optical density (OD) of the plate was read at 450 nanometers (nm) by an Eon Microplate Spectrophotometer (BioTek Instruments Inc., Winooski VT). Data was calculated from a serial dilution curve using Gen5 Data Analysis Software and samples with a coefficient of variation above 15% were eliminated from the analysis.
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bioRxiv - Biochemistry 2021Quote: ... The reaction was stopped with 2M of sulfuric acid and absorbance measured at 450 nm in a Cytation5® plate reader (BioTek, Winooski, VT, USA). The performance of blank ...
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bioRxiv - Microbiology 2022Quote: ... The plate was then cooled to room temperature before the optical density at 562 nm (OD562) was measured in a BioTek Synergy H1 plate reader (BioTek Instruments, Inc, Winooski, VT) after a 5 second orbital shake ...
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bioRxiv - Synthetic Biology 2022Quote: ... 200 mL of a cell suspension at an optical density measured at 600 nm (OD600) of 0.05 were incubated with orbital shaking in a Synergy H1 microtiter plate reader (BioTek Instruments Inc., Winooski, VT, USA) or in an ELx808 microtiter plate reader (BioTek Instruments Inc.) ...
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bioRxiv - Immunology 2023Quote: ... The decreased calcein fluorescence in target cells due to cell lysis was measured at 485 nm excitation wavelength and 528 nm emission wavelength in the bottom reading mode using a Synergy HTX multi-mode plate reader (BioTek, Santa Clara, California, USA). The fluorescence for the experimental condition was adjusted by the parameter γ according to the live target cell control fluorescence ...
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bioRxiv - Plant Biology 2023Quote: ... Absorbance measurements at 649 nm (A649) and 665 nm (A665) were recorded in each well using a plate reader (Biotek Synergy H1; Biotek Instruments, Winooski, VT USA), with triplicates averaged and corrected by the mean of the blank absorbance value ...
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bioRxiv - Microbiology 2023Quote: ... We performed PCA oxidation assays either by tracking the fluorescence of reduced PCA over time in a plate reader (BioTek Synergy 4 or HTX) housed in a Coy-brand anoxic chamber or by tracking the current generated by a culture when provided PCA and an electrode poised to continuously reduce any PCA that the cells oxidized ...
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bioRxiv - Cell Biology 2023Quote: ... the fluorescence of antibodies was measured at 633 nm excitation/665 nm emission using a plate reader (Biotek Synergy H4 or Biotek Synergy Neo2) in technical triplicate as we previously described41 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We then grew all the 96 revived populations in each of the five environments and measured their optical density at 600 nm every 20 minutes using an automated well-plate reader (Synergy HT, BIOTEK® Winooski, VT, USA). The physical conditions during growth measurement were identical to those during the evolution experiment ...
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bioRxiv - Bioengineering 2024Quote: ... Fluorescence was quantified using an excitation of 530/15 nm and emission of 590/15 nm on a fluorescent plate reader (BioTek Cytation 5, Agilent Technologies).
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bioRxiv - Bioengineering 2024Quote: ... DMMB solution was added (200 µL/well) and absorbance was measured at 540 nm and 590 nm using a plate reader (BioTek Cytation 5, Agilent Technologies). For all samples ...
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bioRxiv - Genomics 2020Quote: ... SQ RNA/DNA/Protein Extraction Kit (Omega Biotek Inc.). Briefly ...
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bioRxiv - Immunology 2024Quote: ... HP Total RNA Kit (Omega BioTek, CAT#R6812-02). RNA was reverse transcribed to cDNA using the ABI Reverse Transcription Reagents system ...
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bioRxiv - Microbiology 2024Quote: ... an E.Z.N.A soil DNA kit (Omega Biotek,GA, USA) was used to extract total genomic DNA ...
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bioRxiv - Physiology 2019Quote: ... We calculated T concentration by comparing sample absorbance with the absorbance of the assay’s standard curve (Gen5 curve-fitting software, Biotek EPOCH plate reader, Winooski, VT, USA). Intra-assay CV was 4.44% and inter-plate CV was 5.94%.
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bioRxiv - Microbiology 2020Quote: ... Absorbance at 600 nm of all cultures was measured every 24 hours from 100 μL aliquots dispensed into 96-well flat-bottom plates using an Epoch microplate spectrophotometer (BioTek Instruments Inc. Winooski, VT, USA). Sterile 10% marine broth was used as blank to correct for background media absorbance ...
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bioRxiv - Immunology 2022Quote: ... Reactions were stopped by adding 50 μL 3M hydrochloric acid and absorbance at 492 nm was determined on a Synergy 4 plate reader (BioTek, Agilent Technologies inc., CA, USA) or similar ...
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bioRxiv - Microbiology 2021Quote: The lactobacilli growth rate analysis was performed at 30 and 37°C, under agitation (double orbital, fast mode) in Synergy H1 plate readers (Biotek Instruments, Inc. Winooski, VT, USA). OD was measured every 30 minutes for 24h ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... We calculated T concentration by comparing sample absorbance with the absorbance of the assay’s standard curve (Gen5 curve-fitting software, Biotek EPOCH plate reader, Winooski, VT, USA). Samples from 3 females and 2 males initially showed greater than 80% maximum binding ...
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bioRxiv - Synthetic Biology 2023Quote: ... was measured by exciting the samples with polarized light at 485/20 nm and emission at 528/20 nm at a parallel and perpendicular angle for 40 min with an interval of 5 min to make sure the plateau is maintained using a Synergy 4 plate reader (BioTek Synergy 4, Vermont, United States). Fluorescence polarization was then calculated using the following equation: Fǁ = fluorescence parallel to the excitation plane ...
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bioRxiv - Plant Biology 2023Quote: The aliquot of 100 µL taken from the lipophilic phase of the MTBE extraction was diluted 10-fold with methanol and the extracts were measured under an Epoch2 96-well plate reader (Biotek/Agilent; Santa Clara, CA, U.S.A.). Spectrum was measured at 470 nm ...
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bioRxiv - Synthetic Biology 2024Quote: ... Growth kinetics and related parameters during physiological characterization of engineered strains were derived from measurements of the optical density at 600 nm (OD600) with light path correction in a SynergyTM MX microtiter plate reader (BioTek Instruments Inc., Winooski, VT, USA). For whole-cell 2-pentanone production by engineered P ...
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bioRxiv - Bioengineering 2023Quote: ... Doxorubicin fluorescence (480 nm excitation, 590 nm emission peak) was quantified with the Cytation 5 manager plate reader (BioTek® Instruments, Inc., Winooski, VT, USA). The doxorubicin standard curve was prepared by quantifying the fluorescence of a serial dilution in PBS from 1.0 × 103 to 1.2 × 10−4 µM ...
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bioRxiv - Synthetic Biology 2023Quote: ... and msfGFP fluorescence (lexcitation/lemission = 475 nm/510 nm) were measured along the experiment in a Synergy HI plate reader (BioTek Instruments, Inc., Winooski, VT, USA). Unless stated otherwise ...
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bioRxiv - Cancer Biology 2024Quote: ... and 100 µL of culture supernatant was transferred to a new plate and analyzed for fluorescence intensity using BioTek Synergy H1 Multimode Reader (BioTek Instruments, Inc, Winooski, VT, USA). Percent Specific Lysis was expressed as a percent relative to maximum release control ...
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bioRxiv - Plant Biology 2019Quote: ... Plant RNA kit with DNA digestion on column (Biotek, China) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmids were isolated using a DNA miniprep kit (Omega BioTek) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... or the E.Z.N.A.® HP Total RNA Kit (Omega BioTek). RNA was reverse transcribed to cDNA using the ABI Reverse Transcription Reagents system ...
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bioRxiv - Immunology 2023Quote: ... and E.Z.N.A.® HP Total RNA kit (Omega Biotek, U.S.). RNA samples were sequenced using the Illumina HiSeq platform to generate 50-bp paired-end reads ...
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bioRxiv - Developmental Biology 2022Quote: ... and using Total RNA Kit I (Omega Biotek, R6834-02) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Pure cultures of defined mutants were diluted to A600 0.003 and grown +/− antibiotic in 96-well microtiter format at 37°C with shaking in a plate reader (Tecan M200 Pro, Biotek Epoch 2, or Biotek Synergy H2M). Growth was monitored as change in A600 ...
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bioRxiv - Cell Biology 2023Quote: ... cell culture media from the bottom chamber of the Transwell system was collected (three technical replicates per Transwell) for spectrofluorometric analysis at 490 nm excitation/520 nm emission using a fluorescent plate reader (Biotek Synergy H4 or Biotek Synergy Neo2).
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bioRxiv - Immunology 2020Quote: ... Plasmids were purified using the EZNA plasmid maxi kit (Omega BioTek), according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... and DNA purification and gel extraction kits (Omega Biotek, GA, USA) were used to amplify DNA fragments and purify the PCR products ...
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bioRxiv - Microbiology 2023Quote: ... followed by extraction using E.Z.N.A Viral RNA Kit (R6874, Omega Biotek). Then ...
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bioRxiv - Plant Biology 2023Quote: ... The RNA-prep pure PLANT Kit (DP432, Tiangen Biotek, Beijing, China) was used to isolate total RNA from the rice grains ...
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bioRxiv - Synthetic Biology 2024Quote: ... Amplicons are then purified using EZNA Cycle Pure kits (Omega BioTek). Isothermal assembly followed Gibson Assembly protocols (NEB) ...
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bioRxiv - Plant Biology 2024Quote: ... RNA extraction was performed using E.Z.N.A Total RNA kit (Omega BioTek) following manufacturer directions ...
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bioRxiv - Synthetic Biology 2021Quote: ... The amplicon was then purified using a Cycle Pure Kit (Omega Biotek). The vector backbone fragment was amplified from pET28a-M-CaM-cTdTS plasmid DNA constructed above using PfuUltra II Hotstart PCR Master Mix using DNA primers P29 – P36 listed in Table 1 ...
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bioRxiv - Cell Biology 2022Quote: ... and DNase-treated using commercially available kits (Omega Biotek and Ambion, respectively). Libraries were constructed and subsequently subjected to 151bp paired-end cycles on the NovaSeq-6000 platform (Illumina) ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR product was subsequently cleaned up using PCR cleanup kit (Omega Biotek) and stored in dH2O ...
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bioRxiv - Synthetic Biology 2023Quote: ... The product was subsequently cleaned up using PCR cleanup kit (Omega Biotek) and stored in dH2O ...