Labshake search
Citations for Biotek :
1751 - 1800 of 2058 citations for Total Carbohydrate Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Ion transport modulators differentially modulate inflammatory responses in THP-1 derived macrophagesbioRxiv - Immunology 2021Quote: ... We then measured the absorbance of the resulting azo dye at 540 nm using a multi-mode microplate reader (Synergy™ HTX, Biotek, USA). The amount of NOx produced in each culture was then interpolated from a standard curve generated using sodium nitrite.
-
bioRxiv - Microbiology 2021Quote: ... OD600 values were measured every 10 min for 7 h while plates were grown at 28°C with agitation (205 cpm) in a microplate reader (BioTek SYNERGY H1).
-
bioRxiv - Microbiology 2021Quote: ... Cell suspensions were subsequently distributed into 96-well plates and incubated at 37°C in a BioTek Synergy H1 microplate reader (BioTek, Winooski, VT). Subsequent OD600 measurements were taken every hour for 21 hours ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... and cell viability was measured by a photometer at 595 nm (Synergy™ HTX Multi-Mode Microplate Reader, Biotek, Winooski, VT, USA).
-
bioRxiv - Biochemistry 2021Quote: ... Fluorescent polarization was read parallel and perpendicular to the excitation plane (ex. 485 nm/ em. 528 nm) using a Synergy 2 Microplate reader (BioTek, Winooski, VT) and the relationship between signal and time was linear (Figure S2) ...
-
bioRxiv - Cancer Biology 2020Quote: ... and the plate was returned to incubator for 2 hours after which absorbance was read at 450 nm in a microplate reader (BioTek Instruments Inc).
-
bioRxiv - Bioengineering 2021Quote: ... the media of lower channel was collected and the FITC-dextran concentration was determined by FITC fluorescence intensity using a microplate reader system (BioTek Synergy H1) at 490 nm (Ex ...
-
bioRxiv - Immunology 2021Quote: ... reactions were stopped with 0.16 M sulfuric acid and absorbance was read spectrophotometrically at 450 nm using the ELx808™ Absorbance Microplate Reader (BioTek, Winooski, VT). End-point titers were determined and expressed as the reciprocals of the highest dilution with OD reading above the cut-off value defined as the mean of the control group plus three standard deviations (SD).
-
bioRxiv - Microbiology 2020Quote: ... Growth was assessed by measuring the absorbance at 650 nm (OD650) at 2 h intervals for up to 24 h using an Epoch 2 microplate reader (BioTek, Vermont, USA). The competition assay of F ...
-
bioRxiv - Microbiology 2021Quote: ... 10% SDS solution was added to stop the reaction and the absorbance was measured at 490 nm using a microplate reader (BioTek, Winnooski, VT). Calu-3 cells were seeded in 96-well plate at a density of 40,000 cells/well ...
-
bioRxiv - Immunology 2021Quote: ... and light emission was measured every 2 minutes for a period of 120 min using the Synergy H1 microplate reader (Biotek Instruments, USA). The light emission levels are expressed as RLU (Relative Light Units = luminescence measured by the luminometer).
-
bioRxiv - Microbiology 2021Quote: ... Quantification was performed by measuring soluble A600 CV absorbance using a Biotek Synergy HT microplate reader and Gen 5 software (Biotek, Winooski, VT). Values were normalized to culture growth by dividing by final culture OD600.
-
bioRxiv - Microbiology 2022Quote: ... pH 7.2 at 30 °C) prewarmed to 30 °C and the absorbance at 340 nm was measured immediately using a microplate reader (SynergyMx, BioTek, VT, USA) for 1 h ...
-
bioRxiv - Cell Biology 2022Quote: ... and changes in intracellular Ca2+ concentrations were measured by recording the ratio of fluorescence intensities at 508/20 nm (15 second time interval) using a Synergy 4 multimode microplate reader (BioTek, Winooski, VT). Traces were normalized to their respective baseline intracellular Ca2+ level obtained by averaging the 340/11 nm/380/20 nm ratios of each experiment in the absence of stimulus ...
-
bioRxiv - Immunology 2022Quote: ... on a plate reader with excitation at 585 nm and emission at 530 nm (Synergy H4 Hybrid Microplate Reader, BioTek Instruments, Inc.). Whole-intestine permeability at each time point in each mouse was assessed by subtracting the average relative fluorescence units of baseline plasma and triplicate wells of PBS (sample blank ...
-
bioRxiv - Developmental Biology 2022Quote: ... Plates were maintained at 5% CO2 and 37°C during imaging using Cytation Gen5 Microplate Reader and Imager Software (BioTek, version 3.08.01). Protocols specific to Falcon 24 well companion plates (Corning ...
-
bioRxiv - Plant Biology 2022Quote: ... Chloroplast movement in tomato leaf was monitored by measuring the red light transmittance through leaf discs using a microplate reader (Biotek, Synergy HT) at 25°C as described by Kilambi et al ...
-
bioRxiv - Microbiology 2022Quote: ... The reaction was stopped after 20 min with 100 μL/well of APstopTM solution (KPL Inc.) and absorbance read at 630 nm on a microplate reader (BioTek Instruments Inc). An anti-PvDBP sera was used as negative control ...
-
bioRxiv - Microbiology 2022Quote: ... of the wild-type and mutant strains in 96 well microtiter plates (Surface Nunclon ™ Roskilde, Denmark) using a Sinergy H1 Hybrid Multi-Mode microplate reader (Biotek, USA). For this ...
-
bioRxiv - Plant Biology 2022Quote: ... The DNA was normalized to 5 ng/μL based on quantification with a Synergy 2 multimode microplate reader (Biotek Instruments, Winooski, VT) with the Quant-iT PicoGreen double-stranded DNA quantification assay (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... were incubated in a 96-well flat-bottom plate (Nunclon) at 37°C with continuous shaking (double orbital) in a microplate spectrophotometer (BioTek Epoch 2). Optical densities were recorded every 20 min ...
-
bioRxiv - Neuroscience 2022Quote: ... which used bovine serum albumin standards to create a best fit linear regression line based on protein concentrations using the 562nm absorbance mode of a microplate reader and its Gen5 Data Analysis program (Biotek, Winooski, VT). Equal amounts of protein were resolved in SDS-polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes (Bio-Rad ...
-
bioRxiv - Cancer Biology 2022Quote: ... The optical density (OD) of each well was measured at 510 using a BioTek Synergy™ HT microplate reader (BioTek, Winooski, VT). OD values at 72 h were corrected by 0 h OD subtraction to account for possible variations in initial seeding densities.
-
bioRxiv - Microbiology 2022Quote: ... fluorescence (ex/em = 579/616 nm) and absorbance (600 nm) of each sample were measured using a Synergy H1 microplate reader (BioTek, Vermont, USA). To calculate the relative promoter activity ...
-
bioRxiv - Microbiology 2023Quote: ... The reaction was stopped by adding 50 μl of stop solution and the absorbance was recorded at 450nm using Cytation 5 microplate reader (BioTek Instruments, USA).
-
bioRxiv - Microbiology 2023Quote: ... Plasmin activity was monitored by continuously measuring the rate of change of absorbance at 405 nm for 20 minutes using Cytation 5 microplate reader (BioTek Instruments, USA).
-
bioRxiv - Bioengineering 2023Quote: ... It was then incubated for 1 hour at room temperature before the absorbance was read at 570 nm using a microplate reader (BioTek, Cytation 3). The manufacturer’s standard protocol was used to calibrate the measurements.
-
bioRxiv - Bioengineering 2023Quote: ... All standards were allowed to cool at room temperature under complete darkness and the absorbance was measured at 560 nm using a synergy HTX microplate reader (BioTek, Winooski, VT). The amount of denatured collagen was expressed as the percentage of total amount of collagen.
-
bioRxiv - Plant Biology 2023Quote: ... The background luminescence of seedling was recorded for 2 min before application of flg22 and chitin (5 s for NaCl) on a microplate reader (BioTek Synergy H1). Then 50 μl of 200 nM flg22 ...
-
bioRxiv - Biophysics 2023Quote: ... OD values were read at 450 nm with 570 nm background subtraction using a Powerwave Microplate Spectrophotometer running Gen5 Data Analysis Software (BioTek, Winooski, VT). LDH assay was performed according to the manufacturer’s protocol ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The whole-cell fluorescence was read when the OD600 reached 3.0 with a Synergy HTX multi-mode microplate reader (BioTek, Winooski, VT, USA). Excitation and emission wavelengths were 485 nm and 528 nm ...
-
bioRxiv - Biochemistry 2023Quote: ... roGFP2 fluorescence was recorded for 18 h at 25 °C in a Synergy H1 multi-detection microplate reader (Biotek, Bad Friedrichshall, Germany) at the excitation wavelengths 405 and 488 nm and emission wavelength at 525 nm with the following settings ...
-
bioRxiv - Microbiology 2023Quote: ... was recorded every 1 min over a period of 4 hours at 37°C using the Cytation 5 microplate reader (BioTek Instruments, USA).
-
bioRxiv - Synthetic Biology 2023Quote: ... The absorbance at 405 nm was measured every 1 min for at least 30 min and up to 2 h using a Synergy 4 microplate reader (Biotek, Winooski, VT). SEAP activities were calculated as a function of the increase in absorbance at 405 nm over time42.
-
bioRxiv - Microbiology 2023Quote: ... 2N H2SO4 solution was added to stop the reaction and absorbance was measured at 450 nm using Cytation 5 microplate reader (BioTek Instruments, USA).
-
In vitro impact of fluconazole on oral microbial communities, bacterial growth and biofilm formationbioRxiv - Microbiology 2023Quote: ... The cells were incubated at 37°C and the relative light unit (RLU) and optical density (OD) were measured at various time intervals during growth in a microplate reader (Synergy HT; BioTek, Winooski, VT).
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... acetic acid solution to solubilize the dye incorporated within the cells followed by a spectrophotometric reading at a wavelength of 595 nm using a microplate reader (Synergy HT BioTek, Milan, Italy).
-
bioRxiv - Microbiology 2024Quote: ... NBT was reduced to blueformazan and spectrophotometrically read at OD 650 nm using a Synergy 4 microplate reader (BioTek, Santa Clara, USA). The concentration at which the drugs were able to inhibit 50% parasite growth (IC50 ...
-
bioRxiv - Genomics 2023Quote: ... Both plasma and urine samples were analyzed for urea N in duplicates using a microplate reader (Synergy HTX; BioTek Instruments, Winooski, VT) as described by Jung et al [16] and modified by Zawada et al [17] ...
-
bioRxiv - Developmental Biology 2023Quote: ... The intensity of the color is inversely proportional to the amount of ecdysteroid present as determined by spectrophotometry on a μQuant Microplate Spectrophotometer plate reader at 410 nm (BioTek Instruments, Vermont). The kit solutions were resuspended in UltraPure water (Cayman Chemical) ...
-
bioRxiv - Genetics 2023Quote: ... The plates were sealed with clear plastic adhesive film and incubated at 30°C with no shaking in a microplate spectrophotometer (BioTek Epoch 2) with OD600 readings taken of each well every 30 minutes for 48 hours ...
-
bioRxiv - Microbiology 2024Quote: ... The remaining crystal violet was fully dissolved in 1 ml 95% ethanol with constantly shaking and measured its optical density at 590 nm (Biotek microplate reader). The assay was repeated at least three times with similar results.
-
bioRxiv - Molecular Biology 2024Quote: ... The RNA concentration and purity were assessed using the HTX Synergy Multi-Mode Microplate Reader spectrophotometer (Agilent BioTek, BioTek Instruments Inc, USA). To verify RNA integrity ...
-
bioRxiv - Molecular Biology 2024Quote: ... The RNA concentration and purity were assessed using the HTX Synergy Multi-Mode Microplate Reader spectrophotometer (Agilent BioTek, BioTek Instruments Inc, USA). To verify RNA integrity ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The O.D./min was continuously recorded at 405 nm over 30 min to measure the hydrolytic product 5-mercapto-2-nitrobenzoic acid using a Synergy H1 microplate reader (Biotek; VT, USA). O.D./min was converted to catalytic rate using the Beer-Lambert law:
-
bioRxiv - Neuroscience 2024Quote: ... which used bovine serum albumin standards to create a best fit linear regression line based on protein concentrations using the 562nm absorbance mode of a microplate reader and its Gen5 Data Analysis program (Biotek, Winooski, VT). Equal amounts of protein were resolved in SDS-polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes (Bio-Rad ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Absorbance readings of control and treated wells were taken against a DMSO blank at 570 nm using an Epoch microplate reader (Biotek, Winooski, VT).
-
bioRxiv - Cell Biology 2022Quote: ... both assays were measured with a plate reader (BioTek, Synergy 2) at a 570 nm wavelength ...
-
bioRxiv - Molecular Biology 2021Quote: This assay was performed with two different equipment: Synergy H1 (BioTek) with 96-well plate and LumiCycle luminometer (Actimetrics ...
-
bioRxiv - Microbiology 2022Quote: ... Assay plates were loaded into a Biotek Cytation5 (Biotek, Vermont, USA) pre-equilibrated to 37 °C and 4% CO2 ...