Labshake search
Citations for Biotek :
101 - 150 of 1394 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 4-week-old Arabidopsis plants via a Plant RNA Kit (OMEGA Biotek, Norcross, USA). cDNA was synthesized via ReverTra Ace® qPCR RT Master Mix with gDNA Remover (TOYOBO) ...
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bioRxiv - Bioengineering 2020Quote: ... Well plates were always protected from light and fluorescence measurements were carried out at 37°C and 5% CO2 at Cytation 3 (Cell Imaging Multi-Mode Reader) from Biotek® (USA) ...
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bioRxiv - Cancer Biology 2023Quote: ... The absorbance at 412 nm was measured every 15 sec for 5 min on a Cytation 3 Imaging Reader (BioTek). Total concentration of GSH + GSSG was calculated based on a GSH standard curve.
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bioRxiv - Biochemistry 2024Quote: ... was measured every 10 minutes for 3 hours at 5% CO2 and 37°C using a Synergy H1 microplate reader and CO2 controller (BioTek).
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bioRxiv - Microbiology 2020Quote: ... was added to each well to stop the reaction and the optical density (OD) was measured at 492 nm on a Synergy 4 plate reader (BioTek). An average of OD values for blank wells plus three standard deviations was used to set a cutoff for plate blank outliers ...
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bioRxiv - Microbiology 2020Quote: ... and then optical density and fluorescence were monitored for 48 hours in an Biotek Synergy 4 plate reader (BioTek, USA) at 30 °C with fast continuous shaking ...
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bioRxiv - Cell Biology 2022Quote: ... Intracellular Fluo-4 AM was excited using automatic high-throughput living cell imaging and analysis system (BioTek, Lionheart FX, USA) at 494 nm ...
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bioRxiv - Microbiology 2020Quote: ... the reaction was stopped with 50 μls of 3M hydrochloric acid and absorbance was measured at 490 nm (OD490) using a Synergy 4 (BioTek) plate reader ...
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bioRxiv - Microbiology 2022Quote: ... Fluorescence of released 4-methylumbelliferone (4MU) was measured (Excitation 360 nm, Emission 450 nm) in a plate reader (Synergy MX, Biotek). One unit of chitinase activity releases 1 μmol of 4MU from the appropriate substrate per minute ...
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bioRxiv - Bioengineering 2022Quote: ... Pellet was then resuspended in 1 mL of M9 medium and flasks were inoculated to the starting OD600 of 0.1 and incubated at 30 °C with agitation (233 RPM for cultures with substrate concentration 4 g/L and 275 RPM for cultures with substrate concentration 8 g/L) using NB205 orbital incubator (N-Biotek). The ratio of medium to flask volume was 1:5 (20 mL:100 mL ...
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bioRxiv - Microbiology 2022Quote: ... Plates were incubated at RT for 10 minutes in the dark before luminescence was measured using a Synergy 4 (BioTek) plate reader ...
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bioRxiv - Neuroscience 2023Quote: ... Absorbance at 490 nm was measured 4 hours after the addition of the MTS dye using a SynergyNEO2 plate reader (BioTek).
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bioRxiv - Physiology 2023Quote: ... The cells are first washed from their culture medium by 4 washes of KRB-BSA with the EL406 washer distributor liquid handling device (Biotek) suitable for 384-well plates ...
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bioRxiv - Microbiology 2023Quote: ... The reading process for multiple plates is automated using a BioStack™ 4 microplate stacker (BioTek Instruments, Inc., Winooski, VT), which cycles the experimental 96-well microplates and removes the lids at the specified interval as to not interfere with spectrophotometric values ...
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bioRxiv - Microbiology 2024Quote: ... Plates were stacked without lids and the OD600 was recorded every hour for 24 hours using a stacker (Biostack 4, Agilent BioTek) and plate reader (Epoch 2 ...
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bioRxiv - Neuroscience 2024Quote: ... After 40 hours post-transfection, cells were fixed in 4% PFA, and images (eGFP, mCherry, and eBFP2) were acquired using cytationC10 (Biotek) with a 20x objective ...
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bioRxiv - Microbiology 2024Quote: ... at 37 °C with aeration at 200 rpm until they reached an OD600 between 0.8 and 1.0 (assessed using spectrophotometer BioTek Synergy 4). In the case of low OD600 ...
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bioRxiv - Microbiology 2024Quote: ... Plates were stacked without lids and incubated at 37 °C for 24 h in a stacker-incubator system (Biostack 4, Agilent BioTek) connected to a plate reader (Epoch 2 ...
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bioRxiv - Genetics 2022Quote: ... Brightfield and GFP images were taken every 3 hours for 30 hours using the Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments). GFP quantification was performed by a Cell Profiler program (https://cellprofiler.org/) ...
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bioRxiv - Microbiology 2022Quote: All bacteria were incubated anaerobically in the 24-well plate at 37°C for 3 hours in an Epoch 2 Microplate Spectrophotometer (BioTek), with optical density measured at 600 nm (OD600) ...
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bioRxiv - Neuroscience 2023Quote: ... Fluorescence emission (528, 590 nm) was measured over the course of 3 hours using a Synergy 2 microplate reader (Biotek) with excitation at 485 nm.
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bioRxiv - Molecular Biology 2021Quote: ... The kinetics of substrate cleavage (increase in fluorescence of the liberated 7-amino-4-methylcoumarin, AMC) was measured using a fluorometer (BioTek, USA) set at 370 nm excitation and 460 nm emission at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Endpoint titers were determined by 4-parameter analysis using the Gen5™ 2.0 Data Analysis software (BioTek Instruments, Inc., Winooski, VT) and defined as the reciprocal of the serum dilution producing a signal identical to that of negative control samples at the same dilution plus three times the standard deviation.
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bioRxiv - Biophysics 2021Quote: ... The fluorescence data points were collected for every 5-min interval using a microplate reader (Biotek Synergy 2) with an excitation and emission wavelengths of 440 and 485 nm ...
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bioRxiv - Neuroscience 2021Quote: ... Uptake of MEs labeled with FM 4-64 and RNA cargos labeled with ExoGlow-RNATM was quantified by using a microplate fluorescence reader (BioTek Instruments, Inc.) and confocal microscopy imaging respectively ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 11.1 μl of Alamar blue reagent was added and the readings were taken after 4 hours in a Synergy H1 plate reader (BioTek, Winooski, VT) with 560 nm (excitation ...
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bioRxiv - Cell Biology 2022Quote: ... and changes in intracellular Ca2+ concentrations were measured by recording the ratio of fluorescence intensities at 508/20 nm (15 second time interval) using a Synergy 4 multimode microplate reader (BioTek, Winooski, VT). Traces were normalized to their respective baseline intracellular Ca2+ level obtained by averaging the 340/11 nm/380/20 nm ratios of each experiment in the absence of stimulus ...
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bioRxiv - Microbiology 2022Quote: ... The plate was incubated with shaking at 37 °C and the optical density of each well was measured at a wavelength of 600 nm (BIOTEK, Synergy 4). The MIC values were taken as the lowest concentration for which no growth was discernible (<0.05 OD600 ...
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bioRxiv - Microbiology 2024Quote: ... NBT was reduced to blueformazan and spectrophotometrically read at OD 650 nm using a Synergy 4 microplate reader (BioTek, Santa Clara, USA). The concentration at which the drugs were able to inhibit 50% parasite growth (IC50 ...
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bioRxiv - Microbiology 2023Quote: ... The resulting 1,472 SICs were arranged in 16 96-well plates and incubated in the anaerobic chamber at 37 °C (by setting the temperature of the chamber to 37 °C) in a plate stacker (BioTek BioStack 4) with 2 control plates containing uninoculated media at the top and bottom of the stack to minimize edge effects ...
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bioRxiv - Neuroscience 2023Quote: ... After 40 hours, cells were fixed in 4% PFA and images (eGFP, mCherry and eBFP2) were acquired at cytationC10 (Biotek, Objective 60x). eGFP (interaction signal ...
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bioRxiv - Neuroscience 2023Quote: ... and fluorescence emitted by Atto488 (λex = 485 nm, λem = 538 nm) was measured in a Synergy 4 plate reader (BioTek Instruments GmbH) at intervals of 10 seconds ...
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bioRxiv - Synthetic Biology 2021Quote: ... em 528/20) was collected every 5 minutes with a Synergy 2 multi-mode microplate reader (BioTek, Winooski, VT) for 6 hours at 37 °C.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2 h later the fluorescence was read at 340/505 and 380/505 on a Cytation™ 3 (BioTek (Vermont, MA, USA)) multifunctional microplate reader ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... were added to wells in a microplate and incubated for 2 min at 37°C in a microplate reader (Cytation 3 Imaging Reader, BioTek, VT, USA). Then ...
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bioRxiv - Cell Biology 2022Quote: ... We also performed immunofluorescence staining for pluripotency markers in these iPSC colonies using Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit (Thermo Fischer Scientific) as per manufacturer’s instruction and fluorescence were imaged using fluorescence microscopy (BioTek Lionheart LX Automated Microscope) as described previously.15 Alkaline phosphatase (ALP ...
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bioRxiv - Cell Biology 2021Quote: ... Absorbances were obtained at 620 nm and sample concentrations were determined by comparison to the CXCL12 standards using a 4-parameter curve fit (Synergy HT & Gen5 Software, BioTek Instruments, Winooski, VT). Serum level of CXCL12 was assessed using undiluted serum isolated from the blood of tumor-bearing mice or stage IV glioma patients.
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bioRxiv - Evolutionary Biology 2022Quote: ... and Abs750nm of all 42 plates was measured after 65h exposition (4-6 hours after light on) with an absorbance microplate reader Epoch (BioTek Instruments, VT, USA). 9 data points per well were analyzed and averaged using software Gen5 2.0 (Biotek ...
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bioRxiv - Cancer Biology 2020Quote: ... were added to each well and after 2 hours of incubation at 37°C and 5% CO2 450 nm absorbance was read on a Synergy 2 plate reader (BioTek).
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bioRxiv - Microbiology 2020Quote: ... The cultures were diluted back to a calculated OD 600 of 0.05 (replicated in 2-5 wells for each culture) and their growth measured using an Epoch microplate spectrophotometer (BioTek, VT) at 37 °C with continuous shaking ...
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bioRxiv - Genetics 2020Quote: ... Infected cell frequencies as measured by mNeonGreen expression were assessed at 2 dpi by high content imaging (Cytation 5, BioTek) configured with bright field and GFP cubes ...
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bioRxiv - Microbiology 2023Quote: ... was added to each well and then measured for luminescence using a Synergy 2 Gen 5 plate reader from BioTek.
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bioRxiv - Microbiology 2020Quote: ... on Cytation 3 (BioTek) multimode plate imager according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... on Cytation 3 (BioTek). Virus concentrations that gave signal higher than 105 RLUs/well were used in neutralization experiments ...
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bioRxiv - Microbiology 2021Quote: ... Three replicates of 150 µL of each sample was added to a 96-well plate and incubated with moderate shaking at 37°C (BioTek Synergy 4 Plate Reader) and the OD600 was measured at 30 min intervals for 16 h ...
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bioRxiv - Microbiology 2023Quote: ... We performed PCA oxidation assays either by tracking the fluorescence of reduced PCA over time in a plate reader (BioTek Synergy 4 or HTX) housed in a Coy-brand anoxic chamber or by tracking the current generated by a culture when provided PCA and an electrode poised to continuously reduce any PCA that the cells oxidized ...
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bioRxiv - Bioengineering 2020Quote: ... 5% CO2 using an automated digital microscope with a 10× objective with a gas controller (Cytation 3 microplate reader, BioTek Instruments Inc., Winooski, VT, USA). Images were collected every 10 minutes for 24h ...
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bioRxiv - Microbiology 2022Quote: ... 5 (BioTek). OD600nm readings were taken every hour and growth curves were plotted using GraphPad Prism 9.
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bioRxiv - Immunology 2022Quote: ... Reactions were stopped by adding 50 μL 3M hydrochloric acid and absorbance at 492 nm was determined on a Synergy 4 plate reader (BioTek, Agilent Technologies inc., CA, USA) or similar ...
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bioRxiv - Microbiology 2020Quote: ... UC500) and growth at 37 °C was monitored by measuring optical density at 600 nm every 15 minutes (Biotek Eon instrument with a BioStack 4). Carbon sources tested include D-glucose ...