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Citations for Biotek :
1151 - 1200 of 1300 citations for 7 Boc 3 chloro 5 6 7 8 tetrahydroimidazo 1 2 a pyrazine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Plates were washed 5 times with S Basal then liquid was aspirated using an EL 406 Plate washer / Liquid dispenser (BioTek, VT). 50 μL of 0.01% tween was added to each well to limit worms adhering to pipette tips ...
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bioRxiv - Microbiology 2022Quote: ... Plate was read for luminescence end-point kinetics with a 1s integration on a Cytation 5 Plate Reader using Gen5 software (Biotek Instruments). Relative luminescence units for each siRNA condition were normalized to NTC siRNA and displayed as a percentage.
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bioRxiv - Molecular Biology 2022Quote: ... and incubated at 95 °C for 5 min before the absorbance was measured at 540 nm using a microplate spectrophotometer (BioTek HT).
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bioRxiv - Physiology 2022Quote: ... three representative images of the samples were taken with a Cytation 5 cell imaging multi-mode reader with Gen5 software (Version 3.10; Biotek, Vermont, USA) and analyzed using ImageJ ...
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bioRxiv - Microbiology 2023Quote: ... and measurement of Renilla luciferase activity by adding Stop & Glo® were performed according to the manufacturer instructions using a luminometer reading (Cytation 5 imaging multimode reader, Biotek).
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CRISPR-Cas9 Engineered Extracellular Vesicles for the Treatment of Dominant Progressive Hearing LossbioRxiv - Bioengineering 2023Quote: ... and the resulting emulsion was collected within an Eppendorf tube or the microplate analyzed under the inverted microscope Cytation 5 (BioTek, USA). The isolation of aqueous phase containing RNP-EV from the oil phase was performed under the centrifuge at 2000-3000xg for 5-10min at room temperature ...
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bioRxiv - Biochemistry 2023Quote: PLpro enzyme titration and initial velocity analyses were performed using a Cytation 5 multi-mode microplate reader (BIOTEK Instruments, Winooski, VT), as described previously (Shetler ...
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bioRxiv - Bioengineering 2023Quote: ... the fluorescence of each well was measured at 560/590 nm (excitation/emission) using a microplate reader Cytation 5 (BioTek Instruments). The number of viable cells per well was calculated against a standard curve prepared by plating various concentrations of isolated cells ...
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bioRxiv - Cell Biology 2020Quote: ... values were normalized to the cell count using Hoechst dye in the final prot injection and transferred to the Cytation 5 (BioTek, Winooski, VT).
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bioRxiv - Cell Biology 2019Quote: Cells or spheroids were photographed with a microscope (Zeiss LSM 510) at indicated time points and videos were obtained with a high contrast instrument (Biotek, Cytation 5) for 72 hours.
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bioRxiv - Bioengineering 2020Quote: ... Wells were imaged at time of scratch and 24□h and 48 h later using Gen 5 software (BioTek Instruments, Winooski, VT) on a Cytation 5 plate reader (BioTek Instruments) ...
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bioRxiv - Cell Biology 2022Quote: ... COX activity was determined as the maximal rate of oxidation of fully reduced cytochrome c measured by the change in absorbance over time at 550 nm using an imaging multi-mode reader (BioTek, Cytation 5) at 30 °C ...
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bioRxiv - Microbiology 2020Quote: ... The RNA was quantified by spectrophotometry using a TAKE3 plate in a Cytation 5 multi-mode plate reader (Biotek, Winooski, VT, USA), and RNA quality was assessed by analysis of ribosomal RNA bands visualized following gel electrophoresis by SYBR-safe dye incorporation ...
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bioRxiv - Biochemistry 2021Quote: ... Daily cell number was determined by addition of 20 μl of AqueousOne Solution and measurement of absorbance at 490 nm using a Cytation 5 imaging system (BioTek, Winooski, VT). Fold change was calculated by dividing by absorbance measurements taken at day zero.
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bioRxiv - Microbiology 2021Quote: ... the polymerase activity was monitored by the detection of fluorescence reporter expression on a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, Sursee, Switzerland) equipped with a 4x air objective (numerical aperture (NA) ...
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bioRxiv - Microbiology 2019Quote: ... The cells were stained with DAPI and then visualized by the Cytation 5 Cell Imaging Multi-Mode Reader with objectives 40x PL FL and 20x PL FL (BioTek, Winooski, VT). Image acquisition was performed with BioTek Gen 5 Microplate Reader and Imager Software ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 50 ul of the reaction mixture was added to 200 ul of Stop solution and fluorescence was read using Cytation 5 (Biotek, Winooski, VA) at 360 nm (Excitation ...
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bioRxiv - Microbiology 2021Quote: For the expression level determination of the strains with IPTG‐induced folA‐gfp expression (Fig. 5) we used a combination of plate readers (Biotek Synergy H1) for optical density measurements for growth rate determination (see section growth rate measurements for details ...
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bioRxiv - Microbiology 2021Quote: ... 4 and 5 were performed using two plate readers: A Synergy Neo2 and a Synergy H1 (both from Biotek Inc., VT, USA). Both were set to 30°C with continuous shaking at an orbital displacement of 1 mm and a speed of 807 rpm ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The relative luciferase units (RLUs) in each well were detected using Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, Winooski, VT, USA).
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bioRxiv - Microbiology 2021Quote: ... The growth and lysis kinetics were recorded by measuring the optical density at a wavelength of 600nm (OD600nm) at 37°C with 5-s shaking every 20 min using a 96-well plate reader (Synergy; BioTek, Winooski, VT)
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bioRxiv - Microbiology 2020Quote: ... The reaction was stopped with 3M sulfuric acid 5-10 minutes after OPD addition and absorbance values were measured at a wavelength of 492nm on a spectrophotometer (Biotek Cytation5 system). Absorbance values were normalized by subtracting uncoated wells from antigen-coated wells at the corresponding serum dilution ...
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bioRxiv - Microbiology 2021Quote: ... Quantification was performed by measuring soluble A600 CV absorbance using a Biotek Synergy HT microplate reader and Gen 5 software (Biotek, Winooski, VT). Values were normalized to culture growth by dividing by final culture OD600.
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bioRxiv - Biochemistry 2022Quote: Cy5.5-ALN was characterized in terms of absorbance in the NIR region using a plate reader (Cytation 5, BioTek, Winooski, VT, USA). Yield was calculated using ε675nm=211000 M/cm ...
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bioRxiv - Developmental Biology 2022Quote: ... Plates were maintained at 5% CO2 and 37°C during imaging using Cytation Gen5 Microplate Reader and Imager Software (BioTek, version 3.08.01). Protocols specific to Falcon 24 well companion plates (Corning ...
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bioRxiv - Microbiology 2023Quote: ... The reaction was stopped by adding 50 μl of stop solution and the absorbance was recorded at 450nm using Cytation 5 microplate reader (BioTek Instruments, USA).
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bioRxiv - Microbiology 2023Quote: ... Plasmin activity was monitored by continuously measuring the rate of change of absorbance at 405 nm for 20 minutes using Cytation 5 microplate reader (BioTek Instruments, USA).
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bioRxiv - Microbiology 2023Quote: GFP or mCherry-positive cells were imaged on the Cytation 5 imaging reader and masked using the accompanying Gen5 software (BioTek Instruments, Inc.). The final cell-to-cell spread was calculated as GFP or mCherry-positive cells per area of the entire well ...
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bioRxiv - Bioengineering 2023Quote: ... was quantified by measuring the absorption at 620 nm using a UV/Vis-spectrophotometer (Cytation 5 imaging reader: BioTek, Winooski, VT, USA). A standard curve was used to correlate the absorption value with the amount of HA ...
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bioRxiv - Microbiology 2023Quote: ... 2N H2SO4 solution was added to stop the reaction and absorbance was measured at 450 nm using Cytation 5 microplate reader (BioTek Instruments, USA).
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bioRxiv - Microbiology 2020Quote: ... cells were fixed in 4% PFA and stained with Hoechst nuclear stain before GFP-positive cells were counted using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments, Winooski, VT) with the onboard software for calculations of percentage infected cells.
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bioRxiv - Microbiology 2020Quote: ... Infected cells were then maintained at 37°C for 14-16 h post infection before automatic counting of GFP-positive cells using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments, Winooski, VT) and using the onboard software for calculations of percentage infected cells.
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bioRxiv - Cancer Biology 2021Quote: ... at 1.5×104 cells/ well in 50 μl of DMEM supplemented with 10% FBS using BioTek™ MultiFlo™ FX Microplate Dispensers (BioTek Instruments). Cells were treated with an FDA approved library (Selleckchem ...
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bioRxiv - Immunology 2021Quote: ... Total RNA was quantified using the Take3 microvolume plate accessory on a Cytation 5 Cell Imaging Multi-Mode Reader (Biotek, Winooski, United States), RNA purity was determined by analyzing A260/280 and A260/230 ratios ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... TuCPR activity was measured as the reduction of cytochrome c by calculating absorbance at 550 nm over 5 minutes by with the kinetic mode in a Synergy H1 Hybrid Multi-Mode Reader microplate reader (Biotek, Winooski, VT, USA). The control reaction was the non-enzymatic reduction of cytochrome under the same conditions ...
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bioRxiv - Plant Biology 2020Quote: ... Fluorescence intensity was measured at the vascular bundles of leaves 5 mm from the detachment sight using the Lionheart FX Gen5 image analysis mode (BioTek, Winnoski, VT, USA). Untreated plants were used as control with 6 biological repeats ...
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bioRxiv - Microbiology 2020Quote: ... RNA concentration and quality were measured using a biophotometer and on multimode spot reader using a Cytation™ 5 (BioTek Inc. Winooski, VT). Total RNA from different samples were used to synthesize cDNA using Superscript II reverse transcription kit (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... The plates were then incubated for 1 hour at 37 °C and 5% CO2 and then absorbance at 450 nm was measured using a Synergy H1 Hybrid Multi-Mode Microplate Reader (Biotek, Winooski, VT, USA).
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bioRxiv - Cell Biology 2021Quote: ... isopropyl alcohol to quantitatively measure the density of the stain in different groups at 520 nm using microplate reader (Gene 5 Bio Tek Spectrometer, BioTek, northern Vermont, USA). Isopropanol was used as a blank (26)
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bioRxiv - Neuroscience 2020Quote: ... Immunofluorescence images were acquired using a Cytation 5 Cell Imaging Multi-Mode Reader and analyzed with Gen5™ software (BioTek, Winooski, VT, USA).
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bioRxiv - Cancer Biology 2022Quote: ... The fraction of cells surviving was quantified using either a MacsQuant Analyzer 10 (Milltenyi Biotec, Germany) or by the Cytation 5 system (BioTek, Santa Clara, California). Cell viability was measured according to the manufacturer’s instructions for both instruments ...
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bioRxiv - Biophysics 2022Quote: ... 100 μL of CellTiter-Glo 2.0 was added to each well and luminescence signal was measured in a Cytation 5 imaging reader (BioTek instruments Inc., Winooske, USA) after 10 min incubation.
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bioRxiv - Neuroscience 2023Quote: ... and protein concentrations were determined using the Bradford Assay and an Epoch BioTek Microplate Spectrophotometer (Gen 5 2.01; BioTek Instruments Inc; Winooski, VT). 25 μg of protein from tissue homogenates were combined with specific cytokine antibody-immobilized beads ...
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bioRxiv - Bioengineering 2023Quote: ... The luminescence signal which represents the amount of ATP in viable cells was measured with a microtiter well plate reader (BioTek Cytation 5, Agilent).
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bioRxiv - Bioengineering 2023Quote: ... The fluorescence emission of each well was measured at 593 nm (20 nm bandwidth) with an excitation at 554 nm (18 nm bandwidth) using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments SAS, France). Each condition was replicated three times ...
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bioRxiv - Cancer Biology 2023Quote: ... was supplied by gas controller into the incubator of Cytation 5 multi-mode reader system equilibrated at 37 °C (BioTek; Winooski, VT, USA).
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bioRxiv - Cancer Biology 2023Quote: Fluorescence intensity and absorbance measurements were carried out with Synergy NEO or Cytation 5 multi-mode readers (both from BioTek; Winooski, VT, USA). Bright-field and fluorescence microscopy was carried out with Cytation 5 multi-mode reader using 4× air objective (1.613 µm/pixel ...
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bioRxiv - Microbiology 2023Quote: ... ODs were obtained by measuring absorbance at 600nM in a 96-well plate using Cytation Station 5 (BioTek Agilent Technologies, Santa Clara, CA). 108 colony-forming units (CFUs ...
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bioRxiv - Microbiology 2023Quote: ... ODs were obtained by measuring absorbance at 600nM in a 96-well plate using Cytation Station 5 (BioTek Agilent Technologies, Santa Clara, CA). The liquid cultures were centrifuged at 3500 rpm for 5 minutes (Sorvall Legend X1R M20 rotor ...
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bioRxiv - Cell Biology 2024Quote: ... 2.5 mM calcium chloride for 15 min at room temperature prior to recording fluorescence on a Citation 5 microplate reader (Agilent-BioTek, Santa Clara, CA), exciting at 488 nm and measuring emission at 535 nm ...