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Citations for Biotek :
1101 - 1150 of 1217 citations for 2 Amino 6 chloro 9 3 5 di O p toluoyl beta D 2 deoxyribofurnanosyl purine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Two images per field (NucBlue and Alexa Fluor) and two fields per well were captured on a Cytation 5 cell imaging multimode reader (BioTek) (details in supporting methods and materials) ...
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bioRxiv - Immunology 2022Quote: ... The assay was performed for the next 7 days and each day absorbance was read at 570 nm using Cytation 5 Cell Imaging Multi-Mode Reader (Agilent, BioTek). The assay was performed in triplicate.
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bioRxiv - Microbiology 2022Quote: ... luminescence was quantified with an integration time of 1 s using the Cytation 5 Cell Imaging Multi-Mode Reader (BioTek).
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bioRxiv - Microbiology 2022Quote: ... Cell counts in each well were then determined with whole-well fluorescent imaging using a Cytation 5 Cell Imaging Multi-Mode Reader and Gen5 software (BioTek). In brief ...
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bioRxiv - Synthetic Biology 2022Quote: ... We found that we can remove the opaque substances by photobleaching a 5% Spectracide solution (in LB) in a Synergy H1 plate reader (Biotek), at 30°C and 800 r.p.m ...
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bioRxiv - Cell Biology 2022Quote: ... The absorbance was read at 450 nm (reference at 620 nm) using a Cytation 5 Cell Imaging Multi-Mode Reader (Agilent Biotek).
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bioRxiv - Immunology 2022Quote: ... We measured the cGAMP concentration in the filtrates against a calibration curve of cGAMP at 260 nm using Take3 Micro-Volume absorbance analyzer of Cytation 5 (BioTek). We calculated the final concentration of cGAMP in NanoSTING and encapsulation efficiency by subtracting the concentration of free drug in the filtrate.
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bioRxiv - Immunology 2022Quote: ... The assay plates were then incubated for another 30 min before fluorescence was recorded using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek). Next ...
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bioRxiv - Immunology 2022Quote: ... The assay plates were then incubated for another 30 min before fluorescence was recorded using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek). Next ...
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bioRxiv - Microbiology 2022Quote: ... and measured at an absorbance at 450 nm on a Perkin Elmer Wallac 1420 Victor2TM microplate reader or Cytation 5 cell imaging multi-mode reader (BioTek).
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bioRxiv - Microbiology 2022Quote: ... and measured at an absorbance of 450 nm on a Perkin Elmer Wallac 1420 Victor2TM microplate reader or Cytation 5 cell imaging multi-mode reader (BioTek).
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bioRxiv - Biochemistry 2022Quote: ... Cytotoxicity was evaluated with PrestoBlue Viability Reagent by incubating with cells at 37 °C for 15 minutes and reading fluorescence with excitation 550nm and emission 600nm using a Cytation 5 Plate Reader (BioTek). Luciferase activity was then measured with the Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Cancer Biology 2023Quote: ... Plates were placed on a plate shaker for 30 minutes at room temperature before the luminescence read on a Cytation 5 Imaging Plate Reader (BioTek).
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bioRxiv - Biophysics 2023Quote: ... The bottom of the attractant reservoir was imaged for migrated cells for 8 h with a Cytation 5 BioSpa (Biotek) at 37 °C with 5 % CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... One measure of fluorescence (Ex/Em 560/590 nm) was then read each hour (h) using a Cytation 5 (BioTek) driven by the Gene 5.1.11 Software ...
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bioRxiv - Neuroscience 2024Quote: ... The total RNA concentration was measured using an Eon absorbance microplate reader and Gen 5 software (BioTek, Winooski, VT, USA). The RNA was reverse transcribed using a High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Enzyme activity was monitored by measuring the reduction of NADPH at 340 nm using a plate reader (BioTek, Cytation 5) at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Levels of eGFP in fibroblast cultures infected with recombinant HCMV reporter viruses were measured using a BioTek Cytation 5 (BioTek). High passage HCMV strain Towne was also propagated as described above.
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bioRxiv - Microbiology 2024Quote: ... The OD600 of the samples and dasherGFP abundance (485 ex/528 em) were then measured using a Cytation 5 (Biotek). The transcriptional activity of the rmp promoter was calculated by dividing the dasherGFP fluorescence intensity by the OD600 for each sample.
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bioRxiv - Neuroscience 2024Quote: ... Fluorescence measurements (Ex/Em 535nm/587nm) were made for 30 minutes on a Cytation 5 plate reader (BioTek, Winooski, VT) with PMT gain set at 50 and a read interval of 2 minutes.
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bioRxiv - Immunology 2024Quote: ... Cytotoxicity were measured at 37°C and 5% CO2 every 10 min for 4 h using a Synergy HTX multi-mode plate reader (BioTek) at 485 nm excitation and 528 nm emission wavelengths in the bottom reader mode ...
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bioRxiv - Microbiology 2024Quote: ... Samples were incubated at 95°C for 5 minutes and proteins were quantified using tryptophan fluorescence in a Synergy H1M microplate reader (BioTek) with an excitation wavelength of 280 nm and an emission wavelength of 360 nm ...
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bioRxiv - Synthetic Biology 2024Quote: ... OD600 readings were recorded every 5 minutes for 30 hours at 30 °C using a microplate reader (Biotek, SYNERGY H1), which included a 10-second shake before each measurement ...
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bioRxiv - Cell Biology 2024Quote: ... Fluorescence was read (excitation of 440 and 490 nm, both with emission at 535 nm) on a Cytation 5 (BioTek) plate reader incubated at 37°C with 5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... was added and intensities were measured for each well using a Cytation 3 Multi Modal Reader with Gen5 software (BioTek Instruments, Inc., Winooski, VT, USA). Along with cell viability ...
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bioRxiv - Biochemistry 2024Quote: The pyrene–actin based bulk–polymerization experiments in the absence and presence of VCA– activated Arp2/3 complex were performed at a Synergy 4™ microplate reader (BioTek Instruments, Winooski, VT, USA) using the built–in filter set (Excitation ...
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bioRxiv - Cell Biology 2023Quote: ... the fluorescence was measured at 340 nm excitation wavelength and 480 nm emission wavelength in a Cytation™ 3 microplate reader (BioTek Instruments Inc., Winooski, VT, USA).
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bioRxiv - Cell Biology 2023Quote: ... The fluorescent signal was measured at 548 nm excitation wavelength and 574 nm emission wavelength in a Cytation™ 3 microplate reader (BioTek Instruments Inc., Winooski, VT, USA). As a positive control ...
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bioRxiv - Molecular Biology 2021Quote: ... and wells were washed three times with 300 μl of diluted washing solution (soak time > 5 seconds) using an automated plate washer (BioTek 50TS). After washing ...
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bioRxiv - Neuroscience 2020Quote: ... Fluorescence intensities of the acidic solutions were measured at an excitation/emission of 443/505 nm on A Cytation 5 multimode plate reader (BioTek, Inc). Next ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... and infected cells were maintained at 37°C for 14 to 16 h post infection before manual counting of eGFP+ and mNG+ cells or automated counting using a Cytation 5 cell imaging multi-mode reader (BioTek Instruments) and a CellInsight CX5 imager (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with 2.5μM CellROX for 30min at 37°C after which culture media was replaced for imaging on a BioTek Cytation 5 imaging reader (BioTek, VT). Images were captured from 6 random areas of each well every 10min for 2h ...
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bioRxiv - Plant Biology 2021Quote: ... Absorbance of 50 μl aliquots was determined at 648 and 665 nm using a Cytation 5 plate reader (BioTek, Winooski, VT) and content of chlorophyll a and b per μg wet weight determined using the equations in Barnes et al ...
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bioRxiv - Genetics 2022Quote: ... Brightfield and RFP-channel images were taken every 30 minutes for 12 hours using the Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments). Fluorescence quantifications and the counting of fraction of dead worms were performed by a Cell Profiler program ...
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bioRxiv - Cancer Biology 2022Quote: Cancer cell abundance was observed throughout treatment by imaging mono-cultures and co-culture spheroids using a Cytation 5 cell imaging multimode reader (BioTek Instruments) under dual Bright Field and YFP (ex 500 / em 542 for Venus florescence ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were incubated at room temperature for 10 minutes and read using the Cytation 5 96 well plate reader (BioTek, Vermont) and Gen5 software (version 2.07.17 ...
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bioRxiv - Molecular Biology 2021Quote: ... the firefly LUC and Renilla LUC activities were measured by Dual Luciferase Reporter Gene Assay Kit (Yeasen, Shanghai) and Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, USA). Activity is expressed as the ratio of firefly LUC activity to Renilla LUC activity ...
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bioRxiv - Biochemistry 2020Quote: ... Transfer 100 µL of the solubilized crystal violet to another plate and quantify the absorbance at 550 nm using Cytation™ 5 Cell Imaging Multi-Mode Reader from BioTek.
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bioRxiv - Biochemistry 2020Quote: ... The spectra were recorded using Synergy H1 Hybrid Multi-Mode Reader and Cytation™ 5 Cell Imaging Multi-Mode Reader from BioTek and were averaged by 3 scans.
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bioRxiv - Immunology 2020Quote: ... Samples were incubated for 1 hour at 37°C with 5% CO2 and OD440nm & OD650nm were then measured using a 96-well plate spectrophotometer (Molecular Devices or BioTek). OD650nm was subtracted from OD440nm then percent viability was calculated relative to the no treatment condition.
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bioRxiv - Microbiology 2021Quote: ... coli cells suspensions were adjusted to an optical density at 600 nm (OD600; measured using a UV-visible spectrophotometer, Cytation 5, Biotek Inc.) measurement of 1 ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed 5 times with S Basal then liquid was aspirated using an EL 406 Plate washer / Liquid dispenser (BioTek, VT). 50 μL of 0.01% tween was added to each well to limit worms adhering to pipette tips ...
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bioRxiv - Physiology 2022Quote: ... three representative images of the samples were taken with a Cytation 5 cell imaging multi-mode reader with Gen5 software (Version 3.10; Biotek, Vermont, USA) and analyzed using ImageJ ...
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bioRxiv - Physiology 2024Quote: ... live cells stained with Hoechst 33342 (1:2,000 dilution) (ThermoScientific, Cat#62249) were enumerated using Cytation 5 plate reader (BioTek Instruments - Agilent). Values obtained from the Mito-Stress test were normalized per 1,000 cells.
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bioRxiv - Bioengineering 2023Quote: ... the fluorescence of each well was measured at 560/590 nm (excitation/emission) using a microplate reader Cytation 5 (BioTek Instruments). The number of viable cells per well was calculated against a standard curve prepared by plating various concentrations of isolated cells ...
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CRISPR-Cas9 Engineered Extracellular Vesicles for the Treatment of Dominant Progressive Hearing LossbioRxiv - Bioengineering 2023Quote: ... and the resulting emulsion was collected within an Eppendorf tube or the microplate analyzed under the inverted microscope Cytation 5 (BioTek, USA). The isolation of aqueous phase containing RNP-EV from the oil phase was performed under the centrifuge at 2000-3000xg for 5-10min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Plate was read for luminescence end-point kinetics with a 1s integration on a Cytation 5 Plate Reader using Gen5 software (Biotek Instruments). Relative luminescence units for each siRNA condition were normalized to NTC siRNA and displayed as a percentage.
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bioRxiv - Molecular Biology 2022Quote: ... and incubated at 95 °C for 5 min before the absorbance was measured at 540 nm using a microplate spectrophotometer (BioTek HT).
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bioRxiv - Microbiology 2023Quote: ... and measurement of Renilla luciferase activity by adding Stop & Glo® were performed according to the manufacturer instructions using a luminometer reading (Cytation 5 imaging multimode reader, Biotek).
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bioRxiv - Pathology 2024Quote: ... Alexa 488 fluorescence (485/20, 528/20 nm) or Alexa 647 (635/25, 670/25) was measured by the plate reader Cytation 5 (BioTek, USA).