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Citations for Biotek :
1101 - 1150 of 1205 citations for 2 Amino 3 chloro 5 nitro 6 picoline since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... The fraction of cells surviving was quantified using either a MacsQuant Analyzer 10 (Milltenyi Biotec, Germany) or by the Cytation 5 system (BioTek, Santa Clara, California). Cell viability was measured according to the manufacturer’s instructions for both instruments ...
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bioRxiv - Biophysics 2022Quote: ... 100 μL of CellTiter-Glo 2.0 was added to each well and luminescence signal was measured in a Cytation 5 imaging reader (BioTek instruments Inc., Winooske, USA) after 10 min incubation.
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bioRxiv - Cell Biology 2024Quote: ... 2.5 mM calcium chloride for 15 min at room temperature prior to recording fluorescence on a Citation 5 microplate reader (Agilent-BioTek, Santa Clara, CA), exciting at 488 nm and measuring emission at 535 nm ...
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bioRxiv - Cell Biology 2024Quote: ... 2.5 mM calcium chloride for 15 mins at RT prior to examining fluorescence on a Citation 5 microplate reader (Agilent-BioTek, Santa Clara, CA), exciting at 535 nm and measuring emission at 610 nm ...
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bioRxiv - Neuroscience 2023Quote: ... and protein concentrations were determined using the Bradford Assay and an Epoch BioTek Microplate Spectrophotometer (Gen 5 2.01; BioTek Instruments Inc; Winooski, VT). 25 μg of protein from tissue homogenates were combined with specific cytokine antibody-immobilized beads ...
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bioRxiv - Bioengineering 2023Quote: ... The luminescence signal which represents the amount of ATP in viable cells was measured with a microtiter well plate reader (BioTek Cytation 5, Agilent).
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bioRxiv - Bioengineering 2023Quote: ... The fluorescence emission of each well was measured at 593 nm (20 nm bandwidth) with an excitation at 554 nm (18 nm bandwidth) using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments SAS, France). Each condition was replicated three times ...
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bioRxiv - Microbiology 2023Quote: ... ODs were obtained by measuring absorbance at 600nM in a 96-well plate using Cytation Station 5 (BioTek Agilent Technologies, Santa Clara, CA). 108 colony-forming units (CFUs ...
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bioRxiv - Cancer Biology 2023Quote: Fluorescence intensity and absorbance measurements were carried out with Synergy NEO or Cytation 5 multi-mode readers (both from BioTek; Winooski, VT, USA). Bright-field and fluorescence microscopy was carried out with Cytation 5 multi-mode reader using 4× air objective (1.613 µm/pixel ...
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bioRxiv - Cancer Biology 2023Quote: ... was supplied by gas controller into the incubator of Cytation 5 multi-mode reader system equilibrated at 37 °C (BioTek; Winooski, VT, USA).
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bioRxiv - Microbiology 2023Quote: ... ODs were obtained by measuring absorbance at 600nM in a 96-well plate using Cytation Station 5 (BioTek Agilent Technologies, Santa Clara, CA). The liquid cultures were centrifuged at 3500 rpm for 5 minutes (Sorvall Legend X1R M20 rotor ...
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bioRxiv - Cancer Biology 2024Quote: ... were seeded in 384-well plates in 25 µl of respective culture media using BioTek MultiFlo FX RAD (5 µl cassette) (Biotek, Winooski, VT, USA). Indicated concentrations of small molecules were added for 24 hours ...
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bioRxiv - Bioengineering 2024Quote: ... the absorbance of each well at 540 nm was measured using a Cytation 5 cell imaging multimode reader (Agilent Biotek, Santa Clara, California). The mean absorbance values were used for the calibration curve and the samples ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by detection of the absorbance at 420 and 550 nm at every 2 min using the Viento Nano microplate reader (BioTek Instruments). The relative LacZ activity was calculated as follows ...
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bioRxiv - Physiology 2020Quote: ... The samples were incubated 37 °C for 2 hours and absorbance was measured at 562 nm using a microplate spectrophotometer (BioTek Epoch).
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bioRxiv - Biochemistry 2021Quote: ... The released pNP (extinction coefficient ε=16,000 M-1cm-1) was measured at 405 nm using Epoch 2 plate reader (Biotek, US) set to 37°C and subtracting the blank reading ...
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bioRxiv - Microbiology 2020Quote: ... Promoter activity was then followed by continuous measurement of the growth and GFP fluorescence (one measure every 30 min during 10 h) of the cultures with the Synergy™2 Multi-detection microplate reader (Biotek). Promoter activity was then estimated by dividing the fluorescence value by the OD600 value for each time point.
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bioRxiv - Physiology 2021Quote: ... A 1.5 h kinetics curve was monitored at 37° using a Synergy 2 multi-mode microplate reader (BioTek, Winooski, VT, USA). The absolute amount of cleaved substrate was calculated by an AMC standard curve ...
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bioRxiv - Immunology 2021Quote: ... The reaction was stopped with 50 µL of 2N sulfuric acid and the absorbance at 450 nm was read with an Epoch 2 microplate reader (Biotek, USA).
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bioRxiv - Neuroscience 2021Quote: ... Purified total RNA was quantified on a Synergy 2 Multimode Microplate Reader using a Take3 micro-volume plate (BioTek, Winooski, VT). For cDNA synthesis ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... DNA concentration and purity was monitored by electrophoresis on 2% (w/v) agarose gels and spectrophotometrically by determination of A260/A280 ratios (Synergy HT Microplate Reader, BioTek, USA). Amplicon based metagenomics was performed using the NEBNext® Ultra™ IIDNA Library Prep Kit (Cat No ...
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bioRxiv - Immunology 2020Quote: ... The reaction was stopped with stop solution and absorbance was read at 450 nm using Epoch 2 microplate reader (BioTek, USA). Data are expressed as stimulation index (S.I ...
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bioRxiv - Neuroscience 2021Quote: ... Relative Light Units (RLU) were measured at 28°C using the Synergy 2 SL Microplate Reader with Gen5™ software (BioTek).
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bioRxiv - Cancer Biology 2023Quote: ... Total protein quantification was assayed using the EZQ Protein Quantification Kit (Thermo R33200) according to the manufacturer’s protocol and results were read on a fluorescence-based microplate reader (BioTek Synergy 2). Click chemistry of the biotin-alkyne (PEG4 carboxamide-propargyl biotin ...
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bioRxiv - Biochemistry 2023Quote: ... Fluorescence was measured for 2 h at excitation and emission on a Synergy HTX Multi-Mode Microplate Reader (BioTek, Winooski, VT).
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bioRxiv - Microbiology 2023Quote: ... The cultures were grown under strong agitation at 37°C and analyzed in Synergy 2 Multi-mode microplate reader (BioTek Instruments). Relative Luminescence Units (RLU ...
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bioRxiv - Microbiology 2023Quote: ... transferred to a 96-well plate and incubated at 30°C with double-orbital shaking at 355 cpm in an Epoch 2 microplate reader (BioTek, Germany). Growth was monitored by measuring the OD600 at 10-min intervals until the stationary phase was reached ...
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bioRxiv - Microbiology 2024Quote: ... Optical density measurements at 600 nm were taken every 10 minutes using the Synergy™ 2 Multi-Detection Microplate Reader (BioTek).
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bioRxiv - Cell Biology 2024Quote: ... Samples were incubated 37 °C for 2 h and absorbance was measured at 562 nm using a microplate spectrophotometer (BioTek, Epoch). EV protein yield was determined by multiplying the protein concentration (µg/µL ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated for 24 h at 37 °C in the presence of 5% CO2.with an automated liquid dispenser (BioTek MicroFlo; Thermo-Fisher, Inc.) Drugs/chemicals were dosed in DMSO at 12 concentrations ranging from 50 pM to 50 μM (half-log dilutions ...
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bioRxiv - Microbiology 2023Quote: ... The fluorescence intensity of FM4-64 was measured at 515/20 nm excitation and 635/20 nm emission wavelength using Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, Winooski, VT, United States). A non-coated plate was used with EVs as a control.
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bioRxiv - Bioengineering 2024Quote: ... DMMB solution was added (200 µL/well) and absorbance was measured at 540 nm and 590 nm using a plate reader (BioTek Cytation 5, Agilent Technologies). For all samples ...
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bioRxiv - Bioengineering 2024Quote: ... Fluorescence was quantified using an excitation of 530/15 nm and emission of 590/15 nm on a fluorescent plate reader (BioTek Cytation 5, Agilent Technologies).
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bioRxiv - Immunology 2023Quote: ... Colorimetric immune assays were run in triplicate on 96 well plates using a Cytation 1 cell imaging multi-mode reader and Gen 5 software (BioTek Instruments, Winooski, VT, USA). A Red660 (G Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... All samples were mixed via pipetting before fluorescence was read at 490ex/535em and 440ex/535em after 5 minutes (Biotek SynergyMx with Gen5 software). Background fluorescence from buffer alone was subtracted from each sample before calculating the ratiometric fluorescence of the calibration curve samples and experimental samples ...
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bioRxiv - Neuroscience 2024Quote: ... plates gently shaken for thorough homogenization and incubated for 24 h in standard culture conditions in the Cytation 5 Cell Imaging Multi-mode Reader (BioTek; CAPI – ICB, UFMG, Brazil). Four equally spaced image sets (bright field and RFP channels ...
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bioRxiv - Cell Biology 2024Quote: ... Sixteen sites were acquired per well using 20x Olympus objective plan fluorite NA 0.45 (1320517) with Cytation 5 Hybrid Multidetection Reader (BioTek Instruments, Inc., Winooski, VT, USA). Images from Lysotracker Deep Red were acquired using CY5 led cube (excitation/emission = 628/685 nm) ...
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bioRxiv - Cell Biology 2024Quote: ... Sixteen sites per well were acquired using a 20x Olympus objective plan fluorite NA 0.45 (1320517) in Cytation 5 Hybrid Multidetection Reader (BioTek Instruments, Inc., Winooski, VT, USA), with the incubator on to maintain 37 °C and 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... 25 sites per well were acquired using a 20x Olympus objective plan fluorite NA 0.45 (1320517) in the fluorescence microscope Cytation 5 Hybrid Multidetection Reader (BioTek Instruments, Inc., Winooski, VT, USA), on AO-Green and AO-Red channels.
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bioRxiv - Bioengineering 2020Quote: ... the perfusate from both the fetal and maternal channels was collected and its fluorescence intensity quantified utilizing a microplate reader (BioTek Synergy 2).
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bioRxiv - Evolutionary Biology 2022Quote: ... Chlorophyll a and b were extracted using an acetone and alcohol protocol(Zhao et al., 2018) and quantified using Synergy™2 multi-detection microplate reader (Biotek, VT). Light response curve was measured based on the red and blue light sources using Li 6800 (Li-COR ...
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bioRxiv - Molecular Biology 2021Quote: Fluorescence intensities were measured by a Cary Eclipse spectrofluorometer (Varian, Palo Alto, CA) for single time point measurements and a Synergy 2 plate reader (Biotek, Winooski, VT) for multiple time point measurements.
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bioRxiv - Microbiology 2020Quote: Pure cultures of defined mutants were diluted to A600 0.003 and grown +/− antibiotic in 96-well microtiter format at 37°C with shaking in a plate reader (Tecan M200 Pro, Biotek Epoch 2, or Biotek Synergy H2M). Growth was monitored as change in A600 ...
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bioRxiv - Microbiology 2021Quote: ... The 96-well plates were incubated anaerobically in a temperature-controlled plate reader at 37°C (Epoch 2 Microplate Spectrophotometer, BioTek, Swindon, UK). For each test and technical replicate ...
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bioRxiv - Biochemistry 2020Quote: ... Ten parallel bacterial cultures of 100 μL for each condition were shaken in a Synergy 2 microplate reader (BioTek, Winooski, VT, USA) at 37 oC ...
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bioRxiv - Bioengineering 2021Quote: ... Then the plate was vortexed at 400 rpm for 2 min and luminescence read on a Synergy Neo2 plate reader (BioTek, Winooski, VT).
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bioRxiv - Microbiology 2021Quote: ... The OD600 was used to monitor growth during incubation at 30 °C for 48 h without aeration using a Synergy 2 microplate reader (Biotek, Winooski, VT).
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bioRxiv - Biochemistry 2021Quote: ... and inhibitor (APPI monomer, 0-400 nM; APPI dimer, 0-96 nM) in a Synergy 2 Multi-Detection Microplate Reader (BioTek, VT, USA). Mesotrypsin buffer (296 μL) ...
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bioRxiv - Biochemistry 2021Quote: ... Fluorescent polarization was read parallel and perpendicular to the excitation plane (ex. 485 nm/ em. 528 nm) using a Synergy 2 Microplate reader (BioTek, Winooski, VT) and the relationship between signal and time was linear (Figure S2) ...
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bioRxiv - Cancer Biology 2020Quote: ... and the plate was returned to incubator for 2 hours after which absorbance was read at 450 nm in a microplate reader (BioTek Instruments Inc).