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Citations for Biotek :
1001 - 1050 of 1078 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated for 24 h at 37 °C in the presence of 5% CO2.with an automated liquid dispenser (BioTek MicroFlo; Thermo-Fisher, Inc.) Drugs/chemicals were dosed in DMSO at 12 concentrations ranging from 50 pM to 50 μM (half-log dilutions ...
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bioRxiv - Immunology 2023Quote: ... Colorimetric immune assays were run in triplicate on 96 well plates using a Cytation 1 cell imaging multi-mode reader and Gen 5 software (BioTek Instruments, Winooski, VT, USA). A Red660 (G Biosciences ...
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bioRxiv - Bioengineering 2024Quote: ... Fluorescence was quantified using an excitation of 530/15 nm and emission of 590/15 nm on a fluorescent plate reader (BioTek Cytation 5, Agilent Technologies).
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bioRxiv - Bioengineering 2024Quote: ... DMMB solution was added (200 µL/well) and absorbance was measured at 540 nm and 590 nm using a plate reader (BioTek Cytation 5, Agilent Technologies). For all samples ...
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bioRxiv - Microbiology 2023Quote: ... The fluorescence intensity of FM4-64 was measured at 515/20 nm excitation and 635/20 nm emission wavelength using Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, Winooski, VT, United States). A non-coated plate was used with EVs as a control.
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bioRxiv - Microbiology 2024Quote: ... All samples were mixed via pipetting before fluorescence was read at 490ex/535em and 440ex/535em after 5 minutes (Biotek SynergyMx with Gen5 software). Background fluorescence from buffer alone was subtracted from each sample before calculating the ratiometric fluorescence of the calibration curve samples and experimental samples ...
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bioRxiv - Neuroscience 2024Quote: ... plates gently shaken for thorough homogenization and incubated for 24 h in standard culture conditions in the Cytation 5 Cell Imaging Multi-mode Reader (BioTek; CAPI – ICB, UFMG, Brazil). Four equally spaced image sets (bright field and RFP channels ...
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bioRxiv - Cell Biology 2024Quote: ... Sixteen sites were acquired per well using 20x Olympus objective plan fluorite NA 0.45 (1320517) with Cytation 5 Hybrid Multidetection Reader (BioTek Instruments, Inc., Winooski, VT, USA). Images from Lysotracker Deep Red were acquired using CY5 led cube (excitation/emission = 628/685 nm) ...
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bioRxiv - Cell Biology 2024Quote: ... Sixteen sites per well were acquired using a 20x Olympus objective plan fluorite NA 0.45 (1320517) in Cytation 5 Hybrid Multidetection Reader (BioTek Instruments, Inc., Winooski, VT, USA), with the incubator on to maintain 37 °C and 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... 25 sites per well were acquired using a 20x Olympus objective plan fluorite NA 0.45 (1320517) in the fluorescence microscope Cytation 5 Hybrid Multidetection Reader (BioTek Instruments, Inc., Winooski, VT, USA), on AO-Green and AO-Red channels.
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bioRxiv - Bioengineering 2020Quote: ... the perfusate from both the fetal and maternal channels was collected and its fluorescence intensity quantified utilizing a microplate reader (BioTek Synergy 2).
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bioRxiv - Evolutionary Biology 2022Quote: ... Chlorophyll a and b were extracted using an acetone and alcohol protocol(Zhao et al., 2018) and quantified using Synergy™2 multi-detection microplate reader (Biotek, VT). Light response curve was measured based on the red and blue light sources using Li 6800 (Li-COR ...
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bioRxiv - Molecular Biology 2021Quote: Fluorescence intensities were measured by a Cary Eclipse spectrofluorometer (Varian, Palo Alto, CA) for single time point measurements and a Synergy 2 plate reader (Biotek, Winooski, VT) for multiple time point measurements.
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bioRxiv - Microbiology 2020Quote: ... OD600 recordings were taken every 30 minutes for 44 hours and then every 60 minutes for 96 hours (6 days total) with an Epoch 2 Microplate Spectrophotometer (Biotek, Winooski, VT) at 45°C within a rigid gloveless hypoxic chamber (Coy Lab ...
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bioRxiv - Microbiology 2020Quote: Pure cultures of defined mutants were diluted to A600 0.003 and grown +/− antibiotic in 96-well microtiter format at 37°C with shaking in a plate reader (Tecan M200 Pro, Biotek Epoch 2, or Biotek Synergy H2M). Growth was monitored as change in A600 ...
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bioRxiv - Microbiology 2021Quote: ... The 96-well plates were incubated anaerobically in a temperature-controlled plate reader at 37°C (Epoch 2 Microplate Spectrophotometer, BioTek, Swindon, UK). For each test and technical replicate ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2 h later the fluorescence was read at 340/505 and 380/505 on a Cytation™ 3 (BioTek (Vermont, MA, USA)) multifunctional microplate reader ...
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bioRxiv - Biochemistry 2020Quote: ... Ten parallel bacterial cultures of 100 μL for each condition were shaken in a Synergy 2 microplate reader (BioTek, Winooski, VT, USA) at 37 oC ...
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bioRxiv - Bioengineering 2021Quote: ... Then the plate was vortexed at 400 rpm for 2 min and luminescence read on a Synergy Neo2 plate reader (BioTek, Winooski, VT).
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bioRxiv - Microbiology 2021Quote: ... The OD600 was used to monitor growth during incubation at 30 °C for 48 h without aeration using a Synergy 2 microplate reader (Biotek, Winooski, VT).
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bioRxiv - Biochemistry 2021Quote: ... and inhibitor (APPI monomer, 0-400 nM; APPI dimer, 0-96 nM) in a Synergy 2 Multi-Detection Microplate Reader (BioTek, VT, USA). Mesotrypsin buffer (296 μL) ...
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bioRxiv - Biochemistry 2021Quote: ... Fluorescent polarization was read parallel and perpendicular to the excitation plane (ex. 485 nm/ em. 528 nm) using a Synergy 2 Microplate reader (BioTek, Winooski, VT) and the relationship between signal and time was linear (Figure S2) ...
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bioRxiv - Cancer Biology 2020Quote: ... and the plate was returned to incubator for 2 hours after which absorbance was read at 450 nm in a microplate reader (BioTek Instruments Inc).
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bioRxiv - Microbiology 2020Quote: ... Serosal samples were collected every 30 minutes for 2 hours with FITC dextran flux (ng/ml/cm2/hr) determined by plate reader (Biotek Synergy H1).
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bioRxiv - Immunology 2021Quote: ... and light emission was measured every 2 minutes for a period of 120 min using the Synergy H1 microplate reader (Biotek Instruments, USA). The light emission levels are expressed as RLU (Relative Light Units = luminescence measured by the luminometer).
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bioRxiv - Immunology 2023Quote: ... This was achieved by collecting samples from the serosal compartments every 30 minutes for 2 hours and measuring the respective FITC concentrations on a plate reader (Synergy H1; BioTek, Winooski, VT). Samples with visible FITC leakage in the serosal compartment were omitted ...
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bioRxiv - Genetics 2023Quote: ... The plates were sealed with clear plastic adhesive film and incubated at 30°C with no shaking in a microplate spectrophotometer (BioTek Epoch 2) with OD600 readings taken of each well every 30 minutes for 48 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... were incubated in a 96-well flat-bottom plate (Nunclon) at 37°C with continuous shaking (double orbital) in a microplate spectrophotometer (BioTek Epoch 2). Optical densities were recorded every 20 min ...
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bioRxiv - Plant Biology 2022Quote: ... The reaction was followed by measuring the absorbance at 363 nm at 25°C for 15 min using a plate reader (BioTek Synergy 2). For NR activity measurements on material extracted from Arabidopsis ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... were added to wells in a microplate and incubated for 2 min at 37°C in a microplate reader (Cytation 3 Imaging Reader, BioTek, VT, USA). Then ...
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bioRxiv - Microbiology 2024Quote: ... the OD600 and the relative fluorescence units (RFU) of the cultures were recorded hourly using a Synergy 2 Multi-Mode reader (BioTek, Winooski, VT) and the RFU results were normalized against the OD600 of the culture (82).
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bioRxiv - Microbiology 2024Quote: ... OD660 nm was recorded every 10 min for 24 h at 30 °C under continuous shaking in an Epoch 2 absorbance reader (Biotek Instruments Inc.). When required ...
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bioRxiv - Cell Biology 2024Quote: ... and the samples’ optical density (OD) was measured in a 96 wells plate at 540 nm using a spectrophotometer (Epoch 2, BioTek, Winooski, USA). ODs were corrected by the total number of cells counted (Countess 3 Cell Counter ...
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bioRxiv - Bioengineering 2020Quote: ... 5% CO2 using an automated digital microscope with a 10× objective with a gas controller (Cytation 3 microplate reader, BioTek Instruments Inc., Winooski, VT, USA). Images were collected every 10 minutes for 24h ...
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bioRxiv - Bioengineering 2023Quote: ... Doxorubicin fluorescence (480 nm excitation, 590 nm emission peak) was quantified with the Cytation 5 manager plate reader (BioTek® Instruments, Inc., Winooski, VT, USA). The doxorubicin standard curve was prepared by quantifying the fluorescence of a serial dilution in PBS from 1.0 × 103 to 1.2 × 10−4 µM ...
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A gated hydrophobic funnel within BAX binds long-chain alkenals to potentiate pro-apoptotic functionbioRxiv - Cell Biology 2024Quote: ... Plates were immediately subjected to fluorescence analyses for 90 minutes using either a Synergy H1 or Cytation 5 multi-mode microplate reader (BioTek/Agilent, Santa Clara, CA, USA) using parameters listed in the table below ...
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bioRxiv - Molecular Biology 2024Quote: ... 475 nm) and mCherry (excitation: 587 nm; emission: 610 nm) were measured using the clear bottom read option (SYNERGY H1, BioTek, Gen 5 software, version 3.08.01).
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A gated hydrophobic funnel within BAX binds long-chain alkenals to potentiate pro-apoptotic functionbioRxiv - Cell Biology 2024Quote: ... 0.2 ml of eluate from the indicated fractions was transferred to a black 96 well-plate and analyzed for fluorescence by a Cytation 5 multi-mode plate reader (BioTek/Agilent, Santa Clara, CA, USA) using the following parameters ...
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bioRxiv - Cell Biology 2020Quote: ... The purified extracts of CanlonicSF were excited at 485 ± 20 nm and the intensity of fluorescence emission of cpGFP was recorded at 530 ± 15 nm using the multiwell plate analyzer Synergy 2 (Biotek, Winooski, Vermont, USA). Excitation and emission spectra were obtained by collecting the emissions at 515 ± 15 nm using a multiwell plate reader EnVision (PerkinElmer ...
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bioRxiv - Immunology 2022Quote: ... Reactions were stopped by the addition of 2M sulfuric acid and read using a BioTek Synergy 2 or Synergy H1 plate reader set at 450nm (BioTek, Winooski, VT, USA). Receiver operator curve (ROC ...
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bioRxiv - Physiology 2020Quote: ... The absorbance was determined spectrophotometrically at a wavelength of 450 nm using a spectrophotometer (Epoch 2 microplate spectrophotometer, BioTek Instruments, Inc, Vermont, USA).
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bioRxiv - Biochemistry 2021Quote: We measured the catalytic activity of the enzyme citrate synthase in mitochondrial samples monitoring the conversion of oxaloacetate and acetyl-CoA to citrate and CoA– SH and by measuring the formation of the colorimetric product thionitrobenzoic acid (TNB) at 412 nm and 37°C (Shepherd and Garland, 1969) on a microplate reader (Power Wave XS-2, Biotek Instruments, Winooski, USA). We calculated the enzyme activity using the changes in absorbance after substrate (250 µM oxaloacetate ...
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bioRxiv - Neuroscience 2020Quote: ... Fluorescence post-thallium addition was monitored every 21 seconds for 600 seconds total using a BioTek Synergy 2 (BioTek, Winooski, VT, United States) multi-well plate reader equipped with an excitation filter of 485/20 nm and emission filter 528/20 nm ...
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bioRxiv - Microbiology 2022Quote: ... The cells were grown with agitation at 30°C for 30 hours in a microplate reader (Infinite M Plex; Tecan, Austria, or Synergy 2; BioTek, Winooski, VT, USA), and the optical density at 600 nm (OD600 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Aerobic growth was monitored in technical duplicates or triplicates at 37°C in a BioTek Epoch 2 Microplate Spectrophotometer (BioTek, Bad Friedrichshall, Germany) by absorbance measurements (600nm ...
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bioRxiv - Microbiology 2021Quote: ... was grown at 27°C on a 96-well plate (200 μL) and absorbance at 600 mm was recorded on a microplate reader (Synergy 2 Multi-Mode Microplate Reader, BioTek, Winooski, Vermont, USA). Non-inoculated media were used as blank ...
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bioRxiv - Immunology 2020Quote: ... Growth was measured as relative light units at 528 nm after excitation at 485 nm in a fluorescence microplate reader (Synergy 2, BioTek Instruments, Vermont, USA) at the indicated time points.
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bioRxiv - Genomics 2021Quote: ... absorbance was measured for each well at 435 nm and 545 nm on a Synergy 2 plate reader (Biotek Instruments, Winooski, VT, USA). Because of variation in background absorbance from well to well on each plate ...
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bioRxiv - Genetics 2020Quote: ... Luciferase activity was determined by analyzing 50 μl of each cell extract in a luciferase assay system according to the standard protocol for the Synergy 2 Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA).
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bioRxiv - Immunology 2023Quote: ... AP-conjugated secondary reagents were detected using Lumi-Phos (Lumigen, Southfield, Michigan, USA; 33% solution in water) and a Synergy 2 Luminometer (BioTek, Winooski, Vermont, USA). Washing steps were performed on an ELx405 Select Deep Well Microplate Washer (BioTek ...