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Citations for Biotek :
51 - 100 of 129 citations for Nonanoic acid reaction products with diethanolamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Assay plates were measured for the formazan product at 490 nM using a Synergy 2 plate reader (BioTek, Winooski, VT). Net lactate production was determined by subtracting lactate in the initial media from cancer cell’s lactate production at each time point during the experiments.
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bioRxiv - Immunology 2022Quote: ... and lambda chains.48 The products from the second PCR were analyzed by agarose gel electrophoresis and purified PCR products (ENZA cycle pure kit; Omega Biotek) were submitted to Genewiz for sequencing ...
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bioRxiv - Biochemistry 2024Quote: ... (0-10 mM) and the formation of p-nitrophenol product were monitored at 405 nm on a Synergy H1 microplate reader (BioTek). The bis-pNPP reaction was monitored for 2 h at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... DNA of fecal samples (200 mg) were extracted using Mag-Bind® Universal Metagenomics Kit (Product# M5633-01, Omega Biotek) and DNeasy PowerSoil Kit (Catalog# 12888-100 ...
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bioRxiv - Biochemistry 2020Quote: ... In the linear phase of the reaction (10 – 40 min) turnover rates were calculated using Gen5 software (BioTek) as RFU/min and were normalized by subtracting background hydrolysis rates measured for each substrate in reaction buffer in the absence of protein.
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bioRxiv - Bioengineering 2020Quote: ... Reactions for the fluorescent proteins were carried out in a Synergy Neo2 HTS Multi-Mode Microplate Reader (BioTek) at 282 cpm.
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bioRxiv - Plant Biology 2024Quote: ... The absorbance of the reaction mixture was measured at 630 nm in a microplate reader (Synergy HT, BioTek).
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bioRxiv - Biochemistry 2022Quote: ... Enzyme activity was measured by monitoring the increase in optical density at 235 nm due to formation of unsaturated uronide product using UV/VIS Spectrophotometer (PowerWave Xs2, BioTek, France) during 60 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Absorbance of the MTS-derived formazan product was measured at 490 nm with the Synergy HT plate reader (Biotek, Winooski, VT). Following background subtraction ...
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bioRxiv - Systems Biology 2021Quote: ... 1 mM Dithiothreitol) at 30°C in 100 μl reaction volume using a Synergy H1 plate reader from BioTek, using Corning 3881 96-well half-area clear-bottom non-binding surface plates ...
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bioRxiv - Cell Biology 2020Quote: ... Reactions were incubated for 30 minutes at 37 °C and fluorescence measured using a Synergy H4 microplate reader (BioTek) (Ex/Em = 535/595 nm) ...
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bioRxiv - Immunology 2023Quote: ... the reaction stopped with 1 M HCl and the plates analyzed using a Synergy H1 microplate reader (BioTek Instruments) at 450 nm ...
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bioRxiv - Physiology 2024Quote: ... The final colour change reaction was measured at 450 nm using a Synergy HT microplate reader (Biotek, Vermont, USA). GraphPad Prism software version 9.5.1 for Windows (GraphPad ...
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bioRxiv - Cancer Biology 2021Quote: ... Dimerization of Homovanilic acid was recorded every 30 seconds for 20 minutes with a BioTek Synergy H1 platereader (BioTek) spectrophotometer with an Ex = 320 nm and Em = 420 nm.
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bioRxiv - Immunology 2020Quote: ... After 5 to 10 min the reaction was stopped with 100 µL 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek). A standard curve was generated using rec 2-12C antibody (Absolute Antibody) ...
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bioRxiv - Microbiology 2023Quote: ... and protein concentrations determined using a Bicinchoninic Acid Assay (Pierce BCA Protein Assay, ThermoScientific) on an Eon spectrophotometer (BioTek). Total protein lysates were separated via SDS-PAGE and normalized across the isolates using Stain-FreeTM (BioRad ...
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bioRxiv - Cell Biology 2024Quote: ... Brown adipocytes were incubated in phenol red-free DMEM containing 0.5 mM glucose and 1% fatty acid-free BSA and imaged by a Lionheart FX Automated Microscope (BioTek) with a 10× objective in an environmentally controlled chamber at 37 °C and 5% CO2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Both products were DpnI digested and cleaned with the Viogene Gel/PCR DNA Isolation Kit (Viogene-Biotek Corporation, New Taipei, Taiwan R.O.C.). These fragments were assembled in a Circular Polymerase Extension Cloning (CPEC ...
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bioRxiv - Immunology 2024Quote: ... Fluorescence (λex = 380 nm, λem = 460 nm) of the AMC product was monitored by Cytation 3 Multi-Mode Reader (BioTek, Winooski, VT) (54 ...
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bioRxiv - Microbiology 2020Quote: ... was added to each well to stop the reaction and the optical density (OD) was measured at 492 nm on a Synergy 4 plate reader (BioTek). An average of OD values for blank wells plus three standard deviations was used to set a cutoff for plate blank outliers ...
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bioRxiv - Biochemistry 2021Quote: ... Absorbance was measured in a 200 µl reaction volume using a 96-well plate reader tested by Synergy NEO (BioTeK). Data analysis was performed using GraphPad Prism 5.
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bioRxiv - Microbiology 2020Quote: ... Reactions were initiated by adding 5 μl of 2 mM MUNANA and the fluorescence was measured on Cytation 5 (Biotek) plate reader at 37°C for 10 min using 30 sec intervals and a 365 nm excitation wavelength and a 450 nm emission wavelength ...
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bioRxiv - Synthetic Biology 2022Quote: ... The reactions were next transferred to a 384 conical well plate and incubated in the Synergy H1 plate reader (BioTek) at 30 °C for 3-4 h ...
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bioRxiv - Biochemistry 2020Quote: ... was used as a substrate for the peroxidase and intensity of the reaction was proportional to absorbance measured at 450 nm with SynergyMx (BioTek) after addition of 50 μl H2SO4 at 1M.
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bioRxiv - Developmental Biology 2021Quote: ... the absorbance of the reaction mixture was measured at 520 nm with a microporous plate spectrophotometer (MQX200R2+Take3™, BioTek) according to the user’s manual ...
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bioRxiv - Immunology 2020Quote: ... The colorimetric reaction was measured at OD = 450 nm by a Synergy HTX Multi-Mode Microplate Reader (BioTek Instruments, Inc.).
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bioRxiv - Evolutionary Biology 2023Quote: ... were determined under burst and steady-state conditions using ceftazidime (Δξ = −9,000 M−1 cm−1) at 260 nm by measuring the initial enzymatic reaction rate in a Epoch plate-reader (Biotek). Burst phase rates were determined at 4°C and steady-state parameters were determined at 25°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were incubated in a fluorescence plate reader (most measurements used the Synergy™ H1 hybrid multi-mode reader, BioTek Instruments ...
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bioRxiv - Microbiology 2024Quote: ... was added to start the sialidase reaction and fluorescence 330/440 nm was measured while incubating at 37 °C with shaking in a plate reader (Biotek).
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bioRxiv - Microbiology 2022Quote: ... 96 μL PBS and 4 μL 500 μg/mL nitrocefin (Oxoid) and the reaction was followed by monitoring A486 for 2 h in a microplate reader (Eon Biotek) with readings every 5 min.
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bioRxiv - Neuroscience 2024Quote: ... The reactions were started by the addition of kinase to the reaction mix and the NADH fluorescence was measured using a Synergy H1 microplate reader (Biotek) at 450 nm at 30°C for 10 min with 20 sec intervals ...
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bioRxiv - Synthetic Biology 2024Quote: ... 100 µL reactions were prepared on ice in 96-well plates containing 84 µL nuclease free water (Omega Biotek, PD092), 10 µL of 10X Taq standard buffer (New England Biolabs ...
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bioRxiv - Immunology 2021Quote: ... TMB substrate was added and incubated for 2-5 min and reaction was stopped with 50µl of 1M sulphuric acid and absorbance was measured at 450 and 630nm (reference wavelength) on the Cytation3 Imaging Reader (Biotek). The MN titre was defined as the final dilution of serum that caused ≥50% reduction in NP expression.
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bioRxiv - Immunology 2020Quote: ... substrate the reaction was stopped with 1M sulfuric acid and absorbance was measured at 450 nm and 570 nm (reference wavelength) on the Cytation3 Imaging Reader (Biotek). The MN titers were expressed as half maximal inhibitory dilution (50% Inhibitory titer is ...
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bioRxiv - Microbiology 2021Quote: ... The reaction was stopped by adding 50µL of 3M hydrochloric acid and then absorbance (at 490nm) was immediately determined using Synergy H1 hybrid multimode microplate reader (BioTek). PRISM 7.0 was used to determine the effective concentration of each virus that would yield detectable NA activity ...
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bioRxiv - Microbiology 2022Quote: ... and sulfuric acid and quantified at 450 nm using a BioTek Synergy H1 plate reader (BioTek Instruments Inc., Winoski, VT). Values were analyzed in Prism 9.0 (GraphPad ...
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bioRxiv - Microbiology 2022Quote: ... Wells were washed twice with PBS and stained biofilms were resuspended in 7% acetic acid (v/v) and absorbance was read at 595 nm in a Synergy H1 hybrid multimode reader (BioTek).
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bioRxiv - Microbiology 2020Quote: ... the reaction was stopped with 50 μls of 3M hydrochloric acid and absorbance was measured at 490 nm (OD490) using a Synergy 4 (BioTek) plate reader ...
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bioRxiv - Microbiology 2021Quote: ... stopped using 3M hydrochloric acid (HCL) solution and plates were read at 490nm wavelength using ELISA plate reader (BioTek Instruments). The endpoint antibody titer was determined by using a cut-off value which is three-times the absorbance of first dilution of mock (uninfected ...
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bioRxiv - Immunology 2020Quote: ... After 5 to 10 min the reaction was stopped with 100 µl 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek). The cut off value was defined as the average of all blank wells plus three times the standard deviation of the blank wells.
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bioRxiv - Immunology 2020Quote: ... After 5 to 10 min the reaction was stopped with 100 µl 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek). The cut off value was defined as the average of all blank wells plus three times the standard deviation of the blank wells.
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bioRxiv - Cancer Biology 2023Quote: ... Colonies were solubilized in 10% acetic acid to quantify clonogenic capacity and read for absorbance at 590nm in a plate reader (BioTek).
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bioRxiv - Immunology 2024Quote: ... Reaction was stopped using 0.5 M/1N sulfuric acid and absorbance measured at 450 nm with subtracting reference at 620 nm using ELISA plate reader (Epoc, BioTek). For K18-hACE2 mice challenged with D614G for unspecific binding ...
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bioRxiv - Microbiology 2022Quote: ... The absorbance was quantified at 570 nm using 30% acetic acid in water as the blank by a plate reader (Synergy H1 from BioTek).
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bioRxiv - Biochemistry 2022Quote: ... Reactions were quenched with 2 M sulfuric acid before determining absorbance at 450 nm using a Synergy BioHTX plate reader (BioTek). The background value from wells containing no serum was averaged and subtracted from all wells on the plate.
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bioRxiv - Immunology 2024Quote: ... Plates were developed for 10 minutes at room temperature and then the reaction was stopped by adding 50 μL 3 M hydrochloric acid (HCl, Fisher) and plates were read using a Synergy 4 (BioTek) plate reader at an optical density (OD ...
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bioRxiv - Immunology 2024Quote: ... The reaction was stopped with 100 μL of 1 N sulfuric acid and then read at an absorbance of 450 nm with a Synergy H1 hybrid multimode microplate reader (BioTek). Area under the curve analysis was conducted using GraphPad Prism.
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bioRxiv - Cancer Biology 2024Quote: ... Dried crystals were dissolved in 15% acetic acid and absorbance was measured at 590 nm using an Epoch microplate spectrophotometer (Biotek). Percentage of cell survival was calculated by normalizing the absorbance values of each condition to their respective control in each experiment ...
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bioRxiv - Cancer Biology 2024Quote: ... the staining was eluted with 10% acetic acid solution and the optical density was measured at 570 nm in the spectrophotometer Epoch 2 (Biotek).
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bioRxiv - Cancer Biology 2020Quote: ... absorbance of the soluble formazan product (λ = 490 nm) of MTS reduction was measured on a Synergy H1MF Multi-Mode microplate-reader (BioTek, Winooski, VT), with a reference wavelength of 650 nm to subtract background ...