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Citations for Biotek :
851 - 900 of 1191 citations for tert Butyl 3 5 2 amino ethyl thiophen 3 yl propionate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... Images of each well were captured every 24 hours for the duration of the experiment using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek).
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bioRxiv - Immunology 2022Quote: ... We measured the cGAMP concentration in the filtrates against a calibration curve of cGAMP at 260 nm using Take3 Micro-Volume absorbance analyzer of Cytation 5 (BioTek). We calculated the final concentration of cGAMP in NanoSTING and encapsulation efficiency by subtracting the concentration of free drug in the filtrate.
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bioRxiv - Cancer Biology 2021Quote: ... for 10 min at room temperature and the imaging acquisition was performed by using DAPI 355nm and RFP 558nm on Cytation 5 Cell Imaging Multi-Mode Reader (BioTek). The percentage of positive cells was calculated with a mask function created on the Microscope Software.
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bioRxiv - Cancer Biology 2021Quote: ... The methanol was removed and the CSK buffer was added before imaging nucleus areaacquisition by using DAPI 355nm on Cytation 5 Cell Imaging Multi-Mode Reader (BioTek). The Nucleus area of cells was acquired by a mask function created on the Microscope Software.
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bioRxiv - Cancer Biology 2021Quote: ... Cell imaging acquisition was performed by using GFP 488nm and DAPI 355nm on Cytation 5 Cell Imaging MultiMode Reader (BioTek).
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bioRxiv - Biophysics 2020Quote: ... Fluorescence images of approximately ten thousand cells were acquired per well with a 10x objective in a Cytation 5 (BioTek). The total number of cells ...
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bioRxiv - Microbiology 2020Quote: ... 5 minutes) to remove the beads and the absorbance at 570 nm was measured from the resulting supernatant (Biotek Eon). One million amine plus phosphonate beads were analyzed ...
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bioRxiv - Microbiology 2022Quote: ... 75 µL of culture media was added for each well and the expression of EGFP was monitored every 20 min for 28 hours at 37 °C in Cytation 5 (BioTek) provided with 5% CO2 ...
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bioRxiv - Systems Biology 2022Quote: ... the wells were imaged using the TagBFP (Excitation-390nm, Emission-447nm) and RFP filters (Excitation-531/40 nm, Emission-593/40 nm) in Cytation 5 (Biotek). Each image was flatfield corrected and background subtracted using CellProfiler ...
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bioRxiv - Immunology 2022Quote: ... and the cell plate was sat in dark for 1 h before absorbance at 517 nm was recorded using Cytation 5 Cell Imaging Multi-Mode Reader (BioTek). All values were normalized to the background ...
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bioRxiv - Bioengineering 2020Quote: ... The plate was incubated in the dark at room temperature for 5 minutes followed by fluorescent quantification using an excitation/emission of 485/520 nm (BioTek Cytation5 spectrofluorometric plate reader) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Luminescence at 485 nm and fluorescent eYFP emission at 530 nm were measured for 1 second per well using a Cytation 5 plate Reader (BioTek). The ratio of eYFP/RLuc was calculated per well and data are presented as percent of dopamine response ...
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bioRxiv - Neuroscience 2020Quote: ... The intrinsic tryptophan fluorescence of L-PGDS was measured using Cytation 5 cell imaging multi-mode (Biotek Instrument Inc., USA) reader with λex=295 nm and λem=340 nm ...
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bioRxiv - Immunology 2020Quote: ... Fluorescence levels were recorded every 5 minutes over a period of 500 minutes using a Synergy H1 hybrid reader system (Biotek) at excitation/emission wavelengths of 488/515 nm ...
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bioRxiv - Neuroscience 2021Quote: ... Changes in medium pH during hypercapnia were measured by Phenol Red absorbance at 415 and 560 nm using the Cytation 5 Multi-Mode Reader (BioTek)89 ...
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bioRxiv - Immunology 2020Quote: ... Cells were imaged on a LSM 780 confocal microscope (Zeiss) with a 40 or 63x oil-immersed objective with Airyscan or a Cytation 5 (BioTek) with a 20x objective ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Fluorescence images of approximately ten thousand cells were acquired per well with a 10x objective in a Cytation 5 (BioTek). The total number of cells ...
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bioRxiv - Biochemistry 2021Quote: ... OD600 as well as GFP fluorescence (485 nm excitation, 528 nm emission) was measured in a microplate reader (Cytation 5 BioTek) and the fluorescence signal was normalized to OD600 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stained with Hoechst 33342 and counted for GFP+ and total cells with Cytation 5 high-content live-cell imaging system (Biotek) using a 4x objective.
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bioRxiv - Evolutionary Biology 2021Quote: ... Baseline fluorescence intensity was measured with excitation at 350 nm and emission at 435 nm and 500 nm for 5 min at 37°C on a Synergy H1 spectrophotometer (BioTek). Cells were then treated with 10% ethanol as a membrane fluidizer ...
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bioRxiv - Microbiology 2021Quote: ... Equal volumes of the cell suspension and the BacTiter-Glo reagent were mixed and incubated for 5 min before measuring luminescence using a Synergy Mx Monochromator-Based Multi-Mode Microplate Reader (BioTek). Signal intensities were corrected for cell numbers by dividing the luminescence signal by the OD 595 nm of the cell suspension ...
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bioRxiv - Immunology 2021Quote: ... after 1-5 minutes of adding TMB and absorbance at 450 nm was measured with a Synergy HTX plate reader (BioTek). Concentrations of antigens were determined using standard curves constructed with purified recombinant proteins and calculated with Gen5 3.02.2 (BioTek).
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bioRxiv - Microbiology 2021Quote: ... Sixteen fields were imaged per replicate at 40X using a Cytation 5 plate-reading microscope running Gen5 software (BioTek Instruments). To determine the number of parasites per vacuole ...
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bioRxiv - Immunology 2022Quote: ... The assay was performed for the next 7 days and each day absorbance was read at 570 nm using Cytation 5 Cell Imaging Multi-Mode Reader (Agilent, BioTek). The assay was performed in triplicate.
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bioRxiv - Microbiology 2022Quote: ... luminescence was quantified with an integration time of 1 s using the Cytation 5 Cell Imaging Multi-Mode Reader (BioTek).
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bioRxiv - Microbiology 2022Quote: ... Cell counts in each well were then determined with whole-well fluorescent imaging using a Cytation 5 Cell Imaging Multi-Mode Reader and Gen5 software (BioTek). In brief ...
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bioRxiv - Synthetic Biology 2022Quote: ... We found that we can remove the opaque substances by photobleaching a 5% Spectracide solution (in LB) in a Synergy H1 plate reader (Biotek), at 30°C and 800 r.p.m ...
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bioRxiv - Cell Biology 2022Quote: ... The absorbance was read at 450 nm (reference at 620 nm) using a Cytation 5 Cell Imaging Multi-Mode Reader (Agilent Biotek).
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bioRxiv - Immunology 2022Quote: ... We measured the cGAMP concentration in the filtrates against a calibration curve of cGAMP at 260 nm using Take3 Micro-Volume absorbance analyzer of Cytation 5 (BioTek). We calculated the final concentration of cGAMP in NanoSTING and encapsulation efficiency by subtracting the concentration of free drug in the filtrate.
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bioRxiv - Immunology 2022Quote: ... The assay plates were then incubated for another 30 min before fluorescence was recorded using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek). Next ...
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bioRxiv - Immunology 2022Quote: ... The assay plates were then incubated for another 30 min before fluorescence was recorded using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek). Next ...
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bioRxiv - Microbiology 2022Quote: ... and measured at an absorbance at 450 nm on a Perkin Elmer Wallac 1420 Victor2TM microplate reader or Cytation 5 cell imaging multi-mode reader (BioTek).
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bioRxiv - Microbiology 2022Quote: ... and measured at an absorbance of 450 nm on a Perkin Elmer Wallac 1420 Victor2TM microplate reader or Cytation 5 cell imaging multi-mode reader (BioTek).
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bioRxiv - Biophysics 2022Quote: ... the plate was transferred to a prewarmed (37 °C) and 5 % CO2 Cytation V plate reader (BioTeK, Winooski, United States) and incubated for another 10 mins ...
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bioRxiv - Cell Biology 2024Quote: ... The plates were further incubated for 5 minutes at room temperature before the BRET signal was read on a Synergy Neo (Biotek) equipped with dual photomultiplier tubes (PMTs ...
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bioRxiv - Neuroscience 2024Quote: Microphotographs were obtained with the cell imaging multi-mode plate reader Cytation™ 5 (BioTek Instruments Inc., Winooski, VT, USA) using a green fluorescent protein (GFP ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then incubated for 72 hrs at 37°C in a humidified atmosphere of 5% CO2 before 10 μl of CellTiter-Glo reagent was added to the wells with the MultiFlo Microplate Dispenser (BioTek). Cells were incubated at room temperature for a minimum of 10 minutes after which luminescence readings were recorded by an Infinite M1000 Microplate Reader (Tecan) ...
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bioRxiv - Cell Biology 2024Quote: ... The MTT crystal were solubilized overnight in solubilization solution (10% SDS in 0.01N HCl) and absorbance was measured and at 570 nm and 660 nm was measured using Cytation 5 plate reader (BioTek) 21.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Two images per field (NucBlue and Alexa Fluor) and two fields per well were captured on a Cytation 5 cell imaging multimode reader (BioTek) (details in supporting methods and materials) ...
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bioRxiv - Bioengineering 2024Quote: ... Luminescence intensity from the opaque 384-well plate was measured using a microtiter well plate reader (BioTek® Cytation 5).
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bioRxiv - Cell Biology 2023Quote: ... at an excitation wavelength of 400 nm and an emission wavelength of 505 nm using the Cytation 5 microplate reader (Biotek). Fold-increase in Cathepsin L activity was determined by comparing the relative fluorescence units (RFU ...
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bioRxiv - Synthetic Biology 2023Quote: Fluorescence microscopy was performed with Cytation 5 Cell Imaging Multimode Reader (Bio-Tek) equipped with a BioSpa 8 Automated Microplate Incubator (BioTek). A 20× objective was used to collect bright-field images (autofocusing auto exposure) ...
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bioRxiv - Cancer Biology 2023Quote: ... six wells for each shRNA were stained with 1 μg/mL Hoechst in PBS for 30 minutes and imaged using the Cytation 5 (Biotek) at 4X magnification taking six fields per well ...
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bioRxiv - Neuroscience 2023Quote: ... blue and red fluorescence images were acquired per well at 20X every hour for 12h using the Cytation 5 cell imaging reader (Biotek). Imaging analysis was performed with Gen5 software (Biotek) ...
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bioRxiv - Neuroscience 2023Quote: ... Twenty phase and green fluorescence images were acquired per well from two technical replicates at 20X using the Cytation 5 cell imaging reader (Biotek). Images were acquired immediately after washing and every hour for four hours ...
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bioRxiv - Microbiology 2023Quote: ... The green fluorescent protein (GFP)-positive cells were then counted using Cytation 5 with Gen5 v3.03 software (BioTek, Winooski, VT). Dose-response curves were plotted and EC50 values determined using Prism 9 (GraphPad ...
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bioRxiv - Immunology 2023Quote: ... the changes in the number of fluorescently labeled bacteria and PMNs in the larvae were recorded by taking photos at different time points with a fluorescence microscope (Agilent BioTek Cytation 5, BioTek, Inc. ...
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bioRxiv - Biochemistry 2022Quote: ... Cytotoxicity was evaluated with PrestoBlue Viability Reagent by incubating with cells at 37 °C for 15 minutes and reading fluorescence with excitation 550nm and emission 600nm using a Cytation 5 Plate Reader (BioTek). Luciferase activity was then measured with the Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Cancer Biology 2023Quote: ... Plates were placed on a plate shaker for 30 minutes at room temperature before the luminescence read on a Cytation 5 Imaging Plate Reader (BioTek).
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bioRxiv - Biophysics 2023Quote: ... The bottom of the attractant reservoir was imaged for migrated cells for 8 h with a Cytation 5 BioSpa (Biotek) at 37 °C with 5 % CO2 ...