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Citations for Biotek :
751 - 794 of 794 citations for N Furan 2 ylmethyl 3 bromo 4 fluoro benzamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... ELISA plates were read with a Synergy 2 Gen 5 microplate reader at 405 nm absorbance (BioTek Instruments Inc., Winooski, VT, USA).
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bioRxiv - Cell Biology 2024Quote: ... and the samples’ optical density (OD) was measured in a 96 wells plate at 540 nm using a spectrophotometer (Epoch 2, BioTek, Winooski, USA). ODs were corrected by the total number of cells counted (Countess 3 Cell Counter ...
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bioRxiv - Cell Biology 2020Quote: ... The purified extracts of CanlonicSF were excited at 485 ± 20 nm and the intensity of fluorescence emission of cpGFP was recorded at 530 ± 15 nm using the multiwell plate analyzer Synergy 2 (Biotek, Winooski, Vermont, USA). Excitation and emission spectra were obtained by collecting the emissions at 515 ± 15 nm using a multiwell plate reader EnVision (PerkinElmer ...
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bioRxiv - Immunology 2022Quote: ... Reactions were stopped by the addition of 2M sulfuric acid and read using a BioTek Synergy 2 or Synergy H1 plate reader set at 450nm (BioTek, Winooski, VT, USA). Receiver operator curve (ROC ...
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bioRxiv - Physiology 2020Quote: ... The absorbance was determined spectrophotometrically at a wavelength of 450 nm using a spectrophotometer (Epoch 2 microplate spectrophotometer, BioTek Instruments, Inc, Vermont, USA).
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bioRxiv - Biochemistry 2021Quote: We measured the catalytic activity of the enzyme citrate synthase in mitochondrial samples monitoring the conversion of oxaloacetate and acetyl-CoA to citrate and CoA– SH and by measuring the formation of the colorimetric product thionitrobenzoic acid (TNB) at 412 nm and 37°C (Shepherd and Garland, 1969) on a microplate reader (Power Wave XS-2, Biotek Instruments, Winooski, USA). We calculated the enzyme activity using the changes in absorbance after substrate (250 µM oxaloacetate ...
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bioRxiv - Neuroscience 2020Quote: ... Fluorescence post-thallium addition was monitored every 21 seconds for 600 seconds total using a BioTek Synergy 2 (BioTek, Winooski, VT, United States) multi-well plate reader equipped with an excitation filter of 485/20 nm and emission filter 528/20 nm ...
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bioRxiv - Microbiology 2022Quote: ... The cells were grown with agitation at 30°C for 30 hours in a microplate reader (Infinite M Plex; Tecan, Austria, or Synergy 2; BioTek, Winooski, VT, USA), and the optical density at 600 nm (OD600 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Aerobic growth was monitored in technical duplicates or triplicates at 37°C in a BioTek Epoch 2 Microplate Spectrophotometer (BioTek, Bad Friedrichshall, Germany) by absorbance measurements (600nm ...
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bioRxiv - Microbiology 2021Quote: ... the cells were collected by centrifugation at 10,000 g for 5 min and the absorbance of the supernatant was measured at 562 nm with a Synergy 2 spectrophotometer (BioTek, Winooski, VT, USA). Quantities of ferrihydrite reduced were determined using a standard curve containing a 2-fold range of FeSO4 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... was grown at 27°C on a 96-well plate (200 μL) and absorbance at 600 mm was recorded on a microplate reader (Synergy 2 Multi-Mode Microplate Reader, BioTek, Winooski, Vermont, USA). Non-inoculated media were used as blank ...
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bioRxiv - Cell Biology 2021Quote: ... was measured every 5 min for 2 h at 25°C using Synergy H1 Hybrid Multi-Mode Microplate Reader (BioTek, cat. no. H1MFDG). The rate of kinetic reaction (slope ...
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bioRxiv - Immunology 2020Quote: ... Growth was measured as relative light units at 528 nm after excitation at 485 nm in a fluorescence microplate reader (Synergy 2, BioTek Instruments, Vermont, USA) at the indicated time points.
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bioRxiv - Genomics 2021Quote: ... absorbance was measured for each well at 435 nm and 545 nm on a Synergy 2 plate reader (Biotek Instruments, Winooski, VT, USA). Because of variation in background absorbance from well to well on each plate ...
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bioRxiv - Genetics 2020Quote: ... Luciferase activity was determined by analyzing 50 μl of each cell extract in a luciferase assay system according to the standard protocol for the Synergy 2 Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA).
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bioRxiv - Immunology 2023Quote: ... AP-conjugated secondary reagents were detected using Lumi-Phos (Lumigen, Southfield, Michigan, USA; 33% solution in water) and a Synergy 2 Luminometer (BioTek, Winooski, Vermont, USA). Washing steps were performed on an ELx405 Select Deep Well Microplate Washer (BioTek ...
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bioRxiv - Biochemistry 2023Quote: ... The plates were incubated in the multi-well plate reader instruments (Tecan – Spark/infinite, Crailsheim, Germany or BioTek Epoch 2 - BioTek, Bad Friedrichshall, Germany) at 30 °C ...
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bioRxiv - Physiology 2024Quote: ... Cells were then loaded into individual wells of a white 96-well luminescence plate with an automatic injector unit and luminescence was measured for 20 seconds using a Synergy 2 Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA). Each well of the 96-well plate was pre-loaded with candidate ligands (recombinant ITPa ...
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bioRxiv - Zoology 2024Quote: ... and the luminescent response was measured for 20 seconds followed by additional measurements for 10 seconds after ATP injection to the same well with a Synergy 2 Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA).
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bioRxiv - Evolutionary Biology 2022Quote: ... The OD600 was measured every 2 hours for 24 hours at 37°C and slow continuous shaking using a plate reader (Synergy HT BioTek, Winooski, VT, USA). We used population growth rate and yield as fitness measures (Chavhan et al. ...
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bioRxiv - Synthetic Biology 2022Quote: ... of each well every ∼10 minutes with intermittent orbital and linear shaking in a BioTek Epoch 2 plate reader (BioTek, Bad Friedrichshall, Germany). As had previously been established empirically for the instrument ...
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bioRxiv - Biophysics 2022Quote: ... The amount of inorganic phosphate (Pi) at each time point was determined by taking the absorbance at 620 nm using a plate reader (BioTek Synergy Neo 2) and comparing to a standard Pi concentration plot.
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bioRxiv - Plant Biology 2024Quote: Total chlorophyll was extracted from 20 mg frozen powder with 2 ml methanol and quantified spectrophotometrically at 652 nm and 665 nm with a Synergy HTX microplate reader (BioTek, Bad Friedrichshall, DE). Chlorophyll content was calculated using the equation ...
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bioRxiv - Microbiology 2024Quote: ... FITC concentrations on the basolateral side were quantified by measuring the fluorescence intensity (485 nm excitation and 530 nm emission) in a microplate reader (Synergy-2, BioTek, Winooski, VT, USA). FITC levels were calculated using a standard curve of FITC in DMEM with the indicated supplements ...
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bioRxiv - Microbiology 2024Quote: ... removal by vegetative cells was measured by sampling supernatant and assaying AcA using the change in absorbance at 380 nm in the presence of 2--amino benzamidoxime (ABAO), (as inspired by aldehyde quantifications by Ressmann et. al, 2019) using an Epoch microplate reader (BioTek; Gen5 v3.10 software) [42] ...
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bioRxiv - Physiology 2024Quote: ... The increase in absorbance at 412 nm was monitored over time using a microplate reader (PowerWave XS 2, BioTek Instruments, Winooski, VT, USA). This increase in absorbance indicates the formation of thionitrobenzoic acid ...
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bioRxiv - Microbiology 2021Quote: ... flat-bottomed 96-well plate and the optical density values were read at 726nm on an Epoch 2 BioTek plate reader (BioTek Instruments, Winooski, VT, USA). The standard curve equation was used to determine total polyphenol content of each sample based on absorbance values.
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bioRxiv - Microbiology 2020Quote: ... transferred to 24-well culture plate and the OD600 determined kinetically (1 point/h) using a BioTek Synergy 2 spectrofluorometer (BioTeK U.S., Winooski, VT, USA) at 25°C with continuous slow agitation ...
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bioRxiv - Neuroscience 2020Quote: ... contents were mixed on an orbital shaker for 2 minutes and luminescence readings from experimental samples and ATP standards were acquired using a Synergy H1 microplate reader (BioTek® Instruments, Inc., 253147) and Gen5 Software ...
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bioRxiv - Cell Biology 2022Quote: ... Samples from basolateral chambers were collected at 1 and 2 hours after the start of the experiment and signals from the samples were read by a fluorimeter (BioTek Instruments, Winooski, VT, USA) to determine the amount of dextran passing through the epithelial monolayers.
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bioRxiv - Physiology 2022Quote: ... All assays were run in duplicate under control conditions or in the presence of 5 mM DTT in a 96-well plate and changes in absorbance were tracked using a microplate reader (Synergy 2, BioTek/Agilent, Santa Clara, CA). This concentration was selected based on similar experiments in a previous study [15] ...
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bioRxiv - Physiology 2020Quote: ... The absorbance change vs time for each biomarker was measured spectrophotometrically at a specific wavelength using a spectrophotometer (Epoch 2 microplate spectrophotometer, BioTek Instruments, Inc, Vermont, USA).
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bioRxiv - Biochemistry 2023Quote: ... 250 μl ABTS was mixed with 10 μl of the sample (ethanol and GD extracts) and measured at the wavelength 724 nm using UV/Vis microplate reader (Epoch 2 Microplate Spectrophotometer, BioTek Instruments, Winooski, Vermont, USA). after 15 min of incubation in RT ...
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bioRxiv - Biochemistry 2023Quote: TPC analyses were carried out with the protocol of Singleton and Rossi (Singleton & Rossi, 1965) adopted for microplate reader (Epoch 2 Microplate Spectrophotometer, BioTek Instruments, Winooski, Vermont, USA). Ten microliters of extract ...
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bioRxiv - Microbiology 2023Quote: ... Bacteria were then grown for 24 h at 30°C under continuous shaking in an Epoch 2 Microplate Spectrophotometer from BioTek (Santa Clara, CA, USA). The absorbance at O.D.660 nm was measured every 10 min.
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bioRxiv - Cell Biology 2023Quote: ... after which the formazan crystals formed were dissolved using DMSO and the absorbance of the resulting solution at 570 nm measured on a microplate spectrophotometer (Epoch 2, BioTek company, Gen5 software, USA).
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bioRxiv - Microbiology 2023Quote: ... prior to reading under OD885 against the standard solutions of potassium dihydrogen phosphate (0-2 mg/L) using a microplate reader (EPOCH2, Biotek, Santa Clara, California, USA).
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bioRxiv - Microbiology 2023Quote: Optical density of the microplates is measured at 600 nm (OD600) over 72 hours at an interval of 30 mins using Epoch 2 Microplate Spectrophotometer (BioTek Instruments, Inc., Winooski, VT) with the Corning® 96-well clear flat bottom polystyrene microplate #3370 ...
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bioRxiv - Bioengineering 2024Quote: ... This mixture was incubated at 37°C for 2 h and the absorbance was measured at 562 nm using a SYNERGY HTX multi-mode reader (BioTek Instruments Inc., Winooski, VT).
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bioRxiv - Microbiology 2024Quote: ... The extracted RNA was re-suspended in 30 μl RNase-free water then quantified with a BioTek Synergy™2 Multi-detection Microplate Reader (BioTek Instruments, Winooski, VT) and agarose gel electrophoresis ...
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bioRxiv - Synthetic Biology 2024Quote: ... Quantification of red (mScarlet) fluorescence was performed in a 96-well plate format in an automated Synergy 2 plate reader (BioTek Instruments; Winooski, VT, USA) [53–55] ...
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bioRxiv - Microbiology 2024Quote: ... Biolog Phenotypic Microarray plates (Biolog Inc., Hayward, CA) were inoculated and incubated at 37 °C in a stationary incubator and A490 recorded (BioTek Synergy 2, Winooski, VT, USA). End point readings for carbon sources (PM1 and PM2 plates ...
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bioRxiv - Cell Biology 2020Quote: Total protein levels were calibrated using Bicinchoninic Acid (BCA) assay (ThermoFisher Pierce BCA Protein Assay kit, OD measured with BioTek synergy 2 multi-mode multiplate reader). Samples were ran on a 9% SDS polyacrylamide gel ...
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bioRxiv - Microbiology 2020Quote: Pure cultures of defined mutants were diluted to A600 0.003 and grown +/− antibiotic in 96-well microtiter format at 37°C with shaking in a plate reader (Tecan M200 Pro, Biotek Epoch 2, or Biotek Synergy H2M). Growth was monitored as change in A600 ...