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Citations for Biotek :
751 - 800 of 897 citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and protein concentrations were determined using the Bradford Assay and an Epoch BioTek Microplate Spectrophotometer (Gen 5 2.01; BioTek Instruments Inc; Winooski, VT). 25 μg of protein from tissue homogenates were combined with specific cytokine antibody-immobilized beads ...
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bioRxiv - Bioengineering 2023Quote: ... The luminescence signal which represents the amount of ATP in viable cells was measured with a microtiter well plate reader (BioTek Cytation 5, Agilent).
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bioRxiv - Bioengineering 2023Quote: ... The fluorescence emission of each well was measured at 593 nm (20 nm bandwidth) with an excitation at 554 nm (18 nm bandwidth) using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments SAS, France). Each condition was replicated three times ...
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bioRxiv - Microbiology 2023Quote: ... ODs were obtained by measuring absorbance at 600nM in a 96-well plate using Cytation Station 5 (BioTek Agilent Technologies, Santa Clara, CA). 108 colony-forming units (CFUs ...
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bioRxiv - Cancer Biology 2023Quote: Fluorescence intensity and absorbance measurements were carried out with Synergy NEO or Cytation 5 multi-mode readers (both from BioTek; Winooski, VT, USA). Bright-field and fluorescence microscopy was carried out with Cytation 5 multi-mode reader using 4× air objective (1.613 µm/pixel ...
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bioRxiv - Cancer Biology 2023Quote: ... was supplied by gas controller into the incubator of Cytation 5 multi-mode reader system equilibrated at 37 °C (BioTek; Winooski, VT, USA).
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bioRxiv - Microbiology 2023Quote: ... ODs were obtained by measuring absorbance at 600nM in a 96-well plate using Cytation Station 5 (BioTek Agilent Technologies, Santa Clara, CA). The liquid cultures were centrifuged at 3500 rpm for 5 minutes (Sorvall Legend X1R M20 rotor ...
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bioRxiv - Cancer Biology 2024Quote: ... were seeded in 384-well plates in 25 µl of respective culture media using BioTek MultiFlo FX RAD (5 µl cassette) (Biotek, Winooski, VT, USA). Indicated concentrations of small molecules were added for 24 hours ...
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bioRxiv - Bioengineering 2024Quote: ... the absorbance of each well at 540 nm was measured using a Cytation 5 cell imaging multimode reader (Agilent Biotek, Santa Clara, California). The mean absorbance values were used for the calibration curve and the samples ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated for 24 h at 37 °C in the presence of 5% CO2.with an automated liquid dispenser (BioTek MicroFlo; Thermo-Fisher, Inc.) Drugs/chemicals were dosed in DMSO at 12 concentrations ranging from 50 pM to 50 μM (half-log dilutions ...
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bioRxiv - Microbiology 2023Quote: ... The fluorescence intensity of FM4-64 was measured at 515/20 nm excitation and 635/20 nm emission wavelength using Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, Winooski, VT, United States). A non-coated plate was used with EVs as a control.
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bioRxiv - Bioengineering 2024Quote: ... DMMB solution was added (200 µL/well) and absorbance was measured at 540 nm and 590 nm using a plate reader (BioTek Cytation 5, Agilent Technologies). For all samples ...
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bioRxiv - Bioengineering 2024Quote: ... Fluorescence was quantified using an excitation of 530/15 nm and emission of 590/15 nm on a fluorescent plate reader (BioTek Cytation 5, Agilent Technologies).
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bioRxiv - Microbiology 2024Quote: ... All samples were mixed via pipetting before fluorescence was read at 490ex/535em and 440ex/535em after 5 minutes (Biotek SynergyMx with Gen5 software). Background fluorescence from buffer alone was subtracted from each sample before calculating the ratiometric fluorescence of the calibration curve samples and experimental samples ...
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bioRxiv - Neuroscience 2024Quote: ... plates gently shaken for thorough homogenization and incubated for 24 h in standard culture conditions in the Cytation 5 Cell Imaging Multi-mode Reader (BioTek; CAPI – ICB, UFMG, Brazil). Four equally spaced image sets (bright field and RFP channels ...
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bioRxiv - Cell Biology 2024Quote: ... Sixteen sites were acquired per well using 20x Olympus objective plan fluorite NA 0.45 (1320517) with Cytation 5 Hybrid Multidetection Reader (BioTek Instruments, Inc., Winooski, VT, USA). Images from Lysotracker Deep Red were acquired using CY5 led cube (excitation/emission = 628/685 nm) ...
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bioRxiv - Cell Biology 2024Quote: ... Sixteen sites per well were acquired using a 20x Olympus objective plan fluorite NA 0.45 (1320517) in Cytation 5 Hybrid Multidetection Reader (BioTek Instruments, Inc., Winooski, VT, USA), with the incubator on to maintain 37 °C and 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... 25 sites per well were acquired using a 20x Olympus objective plan fluorite NA 0.45 (1320517) in the fluorescence microscope Cytation 5 Hybrid Multidetection Reader (BioTek Instruments, Inc., Winooski, VT, USA), on AO-Green and AO-Red channels.
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bioRxiv - Synthetic Biology 2023Quote: ... was measured by exciting the samples with polarized light at 485/20 nm and emission at 528/20 nm at a parallel and perpendicular angle for 40 min with an interval of 5 min to make sure the plateau is maintained using a Synergy 4 plate reader (BioTek Synergy 4, Vermont, United States). Fluorescence polarization was then calculated using the following equation: Fǁ = fluorescence parallel to the excitation plane ...
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bioRxiv - Bioengineering 2023Quote: ... Doxorubicin fluorescence (480 nm excitation, 590 nm emission peak) was quantified with the Cytation 5 manager plate reader (BioTek® Instruments, Inc., Winooski, VT, USA). The doxorubicin standard curve was prepared by quantifying the fluorescence of a serial dilution in PBS from 1.0 × 103 to 1.2 × 10−4 µM ...
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A gated hydrophobic funnel within BAX binds long-chain alkenals to potentiate pro-apoptotic functionbioRxiv - Cell Biology 2024Quote: ... Plates were immediately subjected to fluorescence analyses for 90 minutes using either a Synergy H1 or Cytation 5 multi-mode microplate reader (BioTek/Agilent, Santa Clara, CA, USA) using parameters listed in the table below ...
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bioRxiv - Molecular Biology 2024Quote: ... 475 nm) and mCherry (excitation: 587 nm; emission: 610 nm) were measured using the clear bottom read option (SYNERGY H1, BioTek, Gen 5 software, version 3.08.01).
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A gated hydrophobic funnel within BAX binds long-chain alkenals to potentiate pro-apoptotic functionbioRxiv - Cell Biology 2024Quote: ... 0.2 ml of eluate from the indicated fractions was transferred to a black 96 well-plate and analyzed for fluorescence by a Cytation 5 multi-mode plate reader (BioTek/Agilent, Santa Clara, CA, USA) using the following parameters ...
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bioRxiv - Microbiology 2024Quote: Enzymatic activity of AldA was measured by continuously monitoring the spectrophotometric absorbance changes from NAD to NADH (ε340 nm = 6220 M−1 cm−1) at A340nm using an EPOCH2 microplate spectrophotometer (BioTek). Substrate screening experiments were conducted at 25°C with 1 mM of NAD+ and 5 mM of each aldehyde (i.e. ...
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bioRxiv - Evolutionary Biology 2023Quote: ... were determined under burst and steady-state conditions using ceftazidime (Δξ = −9,000 M−1 cm−1) at 260 nm by measuring the initial enzymatic reaction rate in a Epoch plate-reader (Biotek). Burst phase rates were determined at 4°C and steady-state parameters were determined at 25°C ...
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bioRxiv - Genetics 2020Quote: ... 1 µl of OB Protease Solution (Omega BioTek) and 0.2 µl of RNAse A (100 mg/ml) ...
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bioRxiv - Cancer Biology 2024Quote: ... the plates were loaded on CytationTM 1 (BioTek) for cell counting at the GFP channel ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA Kit 1 (Omega BioTek, Norcross, GA). In conditions where we wanted to observe the effects in myotubes ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... a cell imaging multimode reader (BioTek Cytation 1, Agilent BioTek) and an inverted microscope (DMi8 ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... a cell imaging multimode reader (BioTek Cytation 1, Agilent BioTek) and an inverted microscope (DMi8 ...
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bioRxiv - Microbiology 2022Quote: ... Plates were imaged on a Cytation 1 Multimode Plate Reader (BioTek) using a 4X objective lens ...
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bioRxiv - Immunology 2023Quote: ... Cells were imaged using an automated microscope (Cytation 1, Agilent-BioTek). Infected and total cells were counted using a customized pipeline in CellProfiler (Broad Institute ...
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bioRxiv - Microbiology 2021Quote: ... Automated bright field imaging was conducted using a Cytation 1 reader (Biotek) fitted with high contrast bright field imaging capability controlled by Gen5 software (Biotek) ...
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bioRxiv - Microbiology 2021Quote: ... Collection of data was done using the Cytation 1 (BioTek, United States). The OD600 absorbance of all bacterial cultures was measured every 30 min for 24h at a temperature of 37°C and agitation of 355 cpm (cycles per minute) ...
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bioRxiv - Molecular Biology 2022Quote: ... The Luciferase signal was measured using the plate reader (Cytation 1, Biotek).
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bioRxiv - Synthetic Biology 2023Quote: ... for incubation in a Cytation 1 multi-mode plate reader (Agilent BioTek). Plate lids were treated with 10% Triton X-100 in ethanol to prevent fogging (73) ...
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bioRxiv - Immunology 2023Quote: ... Absorption was measured at 405nm on a Cytation 1 plate reader (BioTek). Data was analyzed and plotted with Prism version 10.0.0 (Graphpad).
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bioRxiv - Immunology 2024Quote: ... Absorption was measured with the Cytation 1 (Biotek Instruments, Vermont, United States).
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bioRxiv - Microbiology 2020Quote: ... samples were then processed using the BioTek Cytation 1 Imaging Plate Reader (BioTek).
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bioRxiv - Microbiology 2022Quote: ... Plates were imaged using a Cytation 1 multimode plate reader (Biotek, Winooski, VT). Multiple non-overlapping images were taken for each well to ensure at least 5,000 cells were captured ...
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bioRxiv - Genomics 2023Quote: ... cells were imaged using Cytation™1 Cell Imaging Multi-Mode Reader (BioTek). Green fluorescent channel was used for GFP ...
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bioRxiv - Cell Biology 2020Quote: ... The intensity of the purple color was measured using cytation 1 plate reader (BioTek) at 570 nm ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... ethidium homodimer-1 fluorescence was measured using a Synergy 2 microplate reader (#7131000, BioTek) and Gen5 software (BioTek ...
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bioRxiv - Microbiology 2024Quote: ... The plate was sealed with a Breathe-Easy sealing membrane (Diversified Biotek #BEM-1), and baseline luminescence was measured with a Synergy HTX Multi-Mode Microplate Reader (Agilent ...
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TSG101 Associates with PARP1 and is Essential for PARylation and DNA Damage-induced NF-κB ActivationbioRxiv - Molecular Biology 2021Quote: ... Crystal violet intensities were measured at 570 nm with a plate reader (Cytation 1, Biotek).
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bioRxiv - Bioengineering 2020Quote: ... luminescence was read immediately using a plate reader with a 1 second integration time (BioTek Synergy HT Plate Reader and Gen5 Software ...
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bioRxiv - Microbiology 2024Quote: ... Infected cells (488 nm) and total cells (DAPI) were quantified using the Cytation 1 (Biotek) imaging cytometer ...
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bioRxiv - Genetics 2020Quote: ... and samples were imaged on a Cytation 1 Cell Imaging Multi-Mode Reader (BioTek, Winooski, VT). Images were evaluated using CellProfiler66 and infection efficiencies were calculated from the percentage of virus mRNA positive-cells in each sample ...
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bioRxiv - Genomics 2023Quote: ... The attached monocyte numbers were quantified on Cytation™1 Cell Imaging Multi-Mode Reader (BioTek) using green fluorescent channel ...
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bioRxiv - Synthetic Biology 2024Quote: ... cultures were adjusted to an OD600 of 1 using a Synergy HT Microplate Reader from BioTek, and then diluted 1:100 into fresh media ...