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Citations for Biotek :
651 - 698 of 698 citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... The DNA resulting from this extraction was assessed for quality in a 1% agarose gel and quantified using Synergy™ HT Multi-detection microplate reader (BioTek®, USA). RAD-tag libraries were developed by the Beijing Genomic Institute (BGI ...
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bioRxiv - Immunology 2021Quote: ... The results were measured on the SynergyTM 4 Multi- Mode Microplate Reader with software GEN5CH 2.0 (BioTek, Winooski, VT).
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bioRxiv - Microbiology 2020Quote: ... The 96-well plates were incubated at 37°C for 16 hours using the BioTek synergy 4 (BioTek, USA) plate reader ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial growth was monitored once per hour for 24 h after 30 sec linear shaking (Eon + Biostack 4, Biotek) under anaerobic conditions ...
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bioRxiv - Microbiology 2020Quote: ... the plate was further incubated at room temperature for 4 minutes and read by a spectrophotometer (BioTek, VT, USA) using a 540nm filter.
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 4-week-old Arabidopsis plants via a Plant RNA Kit (OMEGA Biotek, Norcross, USA). cDNA was synthesized via ReverTra Ace® qPCR RT Master Mix with gDNA Remover (TOYOBO) ...
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bioRxiv - Cell Biology 2022Quote: ... lungs were lavaged with 1 ml of sterile PBS to evaluate lung vasculature permeability via microplate reader (495nM ex/519nm em) (Syngery Mx, BioTek Instruments, Inc., Winooski, VT) and tissues taken for histological analysis ...
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bioRxiv - Immunology 2023Quote: ... Colorimetric immune assays were run in triplicate on 96 well plates using a Cytation 1 cell imaging multi-mode reader and Gen 5 software (BioTek Instruments, Winooski, VT, USA). A Red660 (G Biosciences ...
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bioRxiv - Bioengineering 2022Quote: ... The plates were incubated for 15 min at RT on an orbital shaker in the dark and relative luminescence units were measured at 1 s integration time using a Synergy HTX multimode plate reader (BioTek Instruments Inc., Winooski, VT).
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bioRxiv - Microbiology 2020Quote: ... was added to each well to stop the reaction and the optical density (OD) was measured at 492 nm on a Synergy 4 plate reader (BioTek). An average of OD values for blank wells plus three standard deviations was used to set a cutoff for plate blank outliers ...
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bioRxiv - Microbiology 2020Quote: ... and then optical density and fluorescence were monitored for 48 hours in an Biotek Synergy 4 plate reader (BioTek, USA) at 30 °C with fast continuous shaking ...
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bioRxiv - Cell Biology 2022Quote: ... Intracellular Fluo-4 AM was excited using automatic high-throughput living cell imaging and analysis system (BioTek, Lionheart FX, USA) at 494 nm ...
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bioRxiv - Microbiology 2020Quote: ... the reaction was stopped with 50 μls of 3M hydrochloric acid and absorbance was measured at 490 nm (OD490) using a Synergy 4 (BioTek) plate reader ...
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bioRxiv - Cancer Biology 2023Quote: ... incubated for 4 h and fluorescence was read at 550 nm/590 nm on a Cytation 3 Imaging Reader (BioTek).
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bioRxiv - Microbiology 2022Quote: ... Fluorescence of released 4-methylumbelliferone (4MU) was measured (Excitation 360 nm, Emission 450 nm) in a plate reader (Synergy MX, Biotek). One unit of chitinase activity releases 1 μmol of 4MU from the appropriate substrate per minute ...
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bioRxiv - Bioengineering 2022Quote: ... Pellet was then resuspended in 1 mL of M9 medium and flasks were inoculated to the starting OD600 of 0.1 and incubated at 30 °C with agitation (233 RPM for cultures with substrate concentration 4 g/L and 275 RPM for cultures with substrate concentration 8 g/L) using NB205 orbital incubator (N-Biotek). The ratio of medium to flask volume was 1:5 (20 mL:100 mL ...
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bioRxiv - Microbiology 2022Quote: ... Plates were incubated at RT for 10 minutes in the dark before luminescence was measured using a Synergy 4 (BioTek) plate reader ...
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bioRxiv - Neuroscience 2023Quote: ... Absorbance at 490 nm was measured 4 hours after the addition of the MTS dye using a SynergyNEO2 plate reader (BioTek).
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bioRxiv - Physiology 2023Quote: ... The cells are first washed from their culture medium by 4 washes of KRB-BSA with the EL406 washer distributor liquid handling device (Biotek) suitable for 384-well plates ...
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bioRxiv - Microbiology 2023Quote: ... The reading process for multiple plates is automated using a BioStack™ 4 microplate stacker (BioTek Instruments, Inc., Winooski, VT), which cycles the experimental 96-well microplates and removes the lids at the specified interval as to not interfere with spectrophotometric values ...
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bioRxiv - Immunology 2024Quote: ... Plates were developed for 10 minutes at room temperature and then the reaction was stopped by adding 50 μL 3 M hydrochloric acid (HCl, Fisher) and plates were read using a Synergy 4 (BioTek) plate reader at an optical density (OD ...
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bioRxiv - Microbiology 2024Quote: ... Plates were stacked without lids and the OD600 was recorded every hour for 24 hours using a stacker (Biostack 4, Agilent BioTek) and plate reader (Epoch 2 ...
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bioRxiv - Microbiology 2024Quote: ... at 37 °C with aeration at 200 rpm until they reached an OD600 between 0.8 and 1.0 (assessed using spectrophotometer BioTek Synergy 4). In the case of low OD600 ...
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bioRxiv - Immunology 2024Quote: ... Cytotoxicity were measured at 37°C and 5% CO2 every 10 min for 4 h using a Synergy HTX multi-mode plate reader (BioTek) at 485 nm excitation and 528 nm emission wavelengths in the bottom reader mode ...
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bioRxiv - Neuroscience 2024Quote: ... After 40 hours post-transfection, cells were fixed in 4% PFA, and images (eGFP, mCherry, and eBFP2) were acquired using cytationC10 (Biotek) with a 20x objective ...
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bioRxiv - Microbiology 2024Quote: ... Plates were stacked without lids and incubated at 37 °C for 24 h in a stacker-incubator system (Biostack 4, Agilent BioTek) connected to a plate reader (Epoch 2 ...
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bioRxiv - Molecular Biology 2021Quote: ... The kinetics of substrate cleavage (increase in fluorescence of the liberated 7-amino-4-methylcoumarin, AMC) was measured using a fluorometer (BioTek, USA) set at 370 nm excitation and 460 nm emission at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Endpoint titers were determined by 4-parameter analysis using the Gen5™ 2.0 Data Analysis software (BioTek Instruments, Inc., Winooski, VT) and defined as the reciprocal of the serum dilution producing a signal identical to that of negative control samples at the same dilution plus three times the standard deviation.
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bioRxiv - Neuroscience 2021Quote: ... Uptake of MEs labeled with FM 4-64 and RNA cargos labeled with ExoGlow-RNATM was quantified by using a microplate fluorescence reader (BioTek Instruments, Inc.) and confocal microscopy imaging respectively ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 11.1 μl of Alamar blue reagent was added and the readings were taken after 4 hours in a Synergy H1 plate reader (BioTek, Winooski, VT) with 560 nm (excitation ...
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bioRxiv - Microbiology 2021Quote: ... 4 and 5 were performed using two plate readers: A Synergy Neo2 and a Synergy H1 (both from Biotek Inc., VT, USA). Both were set to 30°C with continuous shaking at an orbital displacement of 1 mm and a speed of 807 rpm ...
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bioRxiv - Microbiology 2023Quote: ... The resulting 1,472 SICs were arranged in 16 96-well plates and incubated in the anaerobic chamber at 37 °C (by setting the temperature of the chamber to 37 °C) in a plate stacker (BioTek BioStack 4) with 2 control plates containing uninoculated media at the top and bottom of the stack to minimize edge effects ...
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bioRxiv - Neuroscience 2023Quote: ... After 40 hours, cells were fixed in 4% PFA and images (eGFP, mCherry and eBFP2) were acquired at cytationC10 (Biotek, Objective 60x). eGFP (interaction signal ...
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bioRxiv - Microbiology 2022Quote: ... The plate was incubated with shaking at 37 °C and the optical density of each well was measured at a wavelength of 600 nm (BIOTEK, Synergy 4). The MIC values were taken as the lowest concentration for which no growth was discernible (<0.05 OD600 ...
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bioRxiv - Neuroscience 2023Quote: ... and fluorescence emitted by Atto488 (λex = 485 nm, λem = 538 nm) was measured in a Synergy 4 plate reader (BioTek Instruments GmbH) at intervals of 10 seconds ...
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bioRxiv - Microbiology 2024Quote: ... NBT was reduced to blueformazan and spectrophotometrically read at OD 650 nm using a Synergy 4 microplate reader (BioTek, Santa Clara, USA). The concentration at which the drugs were able to inhibit 50% parasite growth (IC50 ...
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bioRxiv - Cell Biology 2022Quote: ... and changes in intracellular Ca2+ concentrations were measured by recording the ratio of fluorescence intensities at 508/20 nm (15 second time interval) using a Synergy 4 multimode microplate reader (BioTek, Winooski, VT). Traces were normalized to their respective baseline intracellular Ca2+ level obtained by averaging the 340/11 nm/380/20 nm ratios of each experiment in the absence of stimulus ...
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bioRxiv - Cell Biology 2024Quote: ... Signal amplification was performed with HRP-conjugated secondary antibodies (Goat Anti Mouse IgG and Anti Rabbit IgG von Southern Biotek, 130-05 1:8000, Lot: E2518-Z909), diluted 1:8,000 in blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... We also performed immunofluorescence staining for pluripotency markers in these iPSC colonies using Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit (Thermo Fischer Scientific) as per manufacturer’s instruction and fluorescence were imaged using fluorescence microscopy (BioTek Lionheart LX Automated Microscope) as described previously.15 Alkaline phosphatase (ALP ...
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bioRxiv - Cell Biology 2021Quote: ... Absorbances were obtained at 620 nm and sample concentrations were determined by comparison to the CXCL12 standards using a 4-parameter curve fit (Synergy HT & Gen5 Software, BioTek Instruments, Winooski, VT). Serum level of CXCL12 was assessed using undiluted serum isolated from the blood of tumor-bearing mice or stage IV glioma patients.
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bioRxiv - Cancer Biology 2023Quote: ... incubated for 4 h and fluorescence was read at 550 nm/590 nm on a Cytation 3 Imaging Reader (BioTek, Winooski, Vermont, USA).
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bioRxiv - Evolutionary Biology 2022Quote: ... and Abs750nm of all 42 plates was measured after 65h exposition (4-6 hours after light on) with an absorbance microplate reader Epoch (BioTek Instruments, VT, USA). 9 data points per well were analyzed and averaged using software Gen5 2.0 (Biotek ...
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bioRxiv - Microbiology 2021Quote: ... Three replicates of 150 µL of each sample was added to a 96-well plate and incubated with moderate shaking at 37°C (BioTek Synergy 4 Plate Reader) and the OD600 was measured at 30 min intervals for 16 h ...
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bioRxiv - Microbiology 2023Quote: ... We performed PCA oxidation assays either by tracking the fluorescence of reduced PCA over time in a plate reader (BioTek Synergy 4 or HTX) housed in a Coy-brand anoxic chamber or by tracking the current generated by a culture when provided PCA and an electrode poised to continuously reduce any PCA that the cells oxidized ...
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bioRxiv - Immunology 2022Quote: ... Reactions were stopped by adding 50 μL 3M hydrochloric acid and absorbance at 492 nm was determined on a Synergy 4 plate reader (BioTek, Agilent Technologies inc., CA, USA) or similar ...
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bioRxiv - Microbiology 2020Quote: ... UC500) and growth at 37 °C was monitored by measuring optical density at 600 nm every 15 minutes (Biotek Eon instrument with a BioStack 4). Carbon sources tested include D-glucose ...
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bioRxiv - Biochemistry 2024Quote: The pyrene–actin based bulk–polymerization experiments in the absence and presence of VCA– activated Arp2/3 complex were performed at a Synergy 4™ microplate reader (BioTek Instruments, Winooski, VT, USA) using the built–in filter set (Excitation ...
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bioRxiv - Biochemistry 2024Quote: The polymerization and depolymerization of pyrene–labeled recombinant WT or mutant actin (5% pyrene-labeled WT or mutant actin) was monitored as a function of increasing or decreasing pyrene– fluorescence using a Synergy 4™ microplate reader (BioTek Instruments, Winooski, VT, USA) and the built–in filter set (Excitation ...