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Citations for Biotek :
651 - 700 of 898 citations for 3 4 Fluorophenyl 5 fluorobenzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Plates were stacked without lids and the OD600 was recorded every hour for 24 hours using a stacker (Biostack 4, Agilent BioTek) and plate reader (Epoch 2 ...
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bioRxiv - Neuroscience 2024Quote: ... After 40 hours post-transfection, cells were fixed in 4% PFA, and images (eGFP, mCherry, and eBFP2) were acquired using cytationC10 (Biotek) with a 20x objective ...
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bioRxiv - Microbiology 2024Quote: ... at 37 °C with aeration at 200 rpm until they reached an OD600 between 0.8 and 1.0 (assessed using spectrophotometer BioTek Synergy 4). In the case of low OD600 ...
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bioRxiv - Microbiology 2024Quote: ... Plates were stacked without lids and incubated at 37 °C for 24 h in a stacker-incubator system (Biostack 4, Agilent BioTek) connected to a plate reader (Epoch 2 ...
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bioRxiv - Biochemistry 2020Quote: ... 30 μL of a 10 nM solution of FphF in PBS/0.01%TritonX-100 was added and fluorescence (λex = 365 nm and λem = 455 nm) was read at 37°C in 1 min intervals on a Cytation 3 imaging reader (BioTek, Winooski, VT, USA) for 60 min ...
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bioRxiv - Cell Biology 2022Quote: ... The reduction of resazurin to resorufin was measured fluorometrically at 540 nm excitation and 590 nm emission in a Cytation 3 microplate reader (BioTek Instruments Inc., USA). The results were expressed as % of untreated cells ...
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bioRxiv - Microbiology 2020Quote: ... Em = 450 nm) over a period of 30-60 minutes at 37°C on a Cytation-3 imaging reader (BioTek, Winooski, VT, USA). Concentrations of hydrolyzed products were determined using a standard concentration curve (unconjugated 4MU) ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were incubated at 37°C and fluorescence was measured (Ex = 410 nm, Em = 450 nm) over a period of 60 minutes on a Cytation 3 imaging reader (BioTek, Winooski, VT, USA). Reaction rates were determined by linear regression analysis of the initial linear phase of hydrolysis ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Relative dopaminergic cell viability was assessed by counting MAP2- and TH-positive neurons in a blinded manner using images taken with an automated Cytation 3 Cell Imaging Reader (BioTek Instruments, Winooski, VT) equipped with a 4X objective ...
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bioRxiv - Microbiology 2023Quote: ... and inhibitor simultaneously Cleavage was measured using fluorescence (EX 380 nm/EM 460 nm) with Cytation 3 imaging reader (BioTek, Winooski, VT, USA) for 60 minutes and slope was used to calculate the inhibition curve ...
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bioRxiv - Synthetic Biology 2023Quote: ... 80 µL ONE-Glo EX Reagent was then added and the plate was orbitally shaken at 300 rpm for 3 min in a microplate reader (BioTek Synergy H1, v2.04.11). Firefly luminescence was collected using the microplate reader’s luminescence fiber ...
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bioRxiv - Molecular Biology 2021Quote: ... The kinetics of substrate cleavage (increase in fluorescence of the liberated 7-amino-4-methylcoumarin, AMC) was measured using a fluorometer (BioTek, USA) set at 370 nm excitation and 460 nm emission at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Endpoint titers were determined by 4-parameter analysis using the Gen5™ 2.0 Data Analysis software (BioTek Instruments, Inc., Winooski, VT) and defined as the reciprocal of the serum dilution producing a signal identical to that of negative control samples at the same dilution plus three times the standard deviation.
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bioRxiv - Cancer Biology 2022Quote: ... was added to a final concentration of 0.1 mg/ml and incubated for 5 h prior to measuring the excitation/emission (544/590) (Biotek). Cells were reverse transfected (Lipofectamine 3000 ...
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bioRxiv - Molecular Biology 2021Quote: The dual-luciferase assay was performed using a Cytation™ 5 plate reader with a dual-injector system (BioTek). For each sample ...
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bioRxiv - Biochemistry 2020Quote: ... The absorbance at 600 nm was monitored over time using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek) at 5 min time intervals for up to 12 hr with mixing ...
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bioRxiv - Bioengineering 2021Quote: ... Sample fluorescence (Ex/Em = 488 nm/525 nm) were measured for 180 minutes using Cytation 5 plate reader (Biotek). Enzyme cleavage rates were quantified as relative fluorescence increase over time normalized to fluorescence before addition of protease ...
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bioRxiv - Cell Biology 2021Quote: ... The kinetics of the GTP hydrolysis was continuously monitored by the absorbance at 360 nm in Cytation 5 (BioTek) at 22 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... em 528/20) was collected every 5 minutes with a Synergy 2 multi-mode microplate reader (BioTek, Winooski, VT) for 6 hours at 37 °C.
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bioRxiv - Cell Biology 2021Quote: ... The absorbance at 600 nm was monitored over time using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek) at 5 min time intervals for up to 12 h with mixing and subtracted from a blank buffer with no turbidity.
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bioRxiv - Cancer Biology 2022Quote: ... HPTS and RuBPY fluorescence were monitored for 17 h using a Cytation 5 device (BioTek, Agilent, Winooski, VT, USA). Excitation was provided by a monochromator ...
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bioRxiv - Synthetic Biology 2022Quote: ... Plasmid concentrations were quantified using the Cytation 5 plate reader with the Take3 Micro-Volume Plate (BioTek, Winooski, VT) and all fell in the range of 100-200 ng/μL ...
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bioRxiv - Microbiology 2022Quote: ... The cell plate was infected with virus at approximately 0.5 TCID50/well and incubated at 37 °C in the Cytation 5 (BioTek) supplemented with 5% CO2 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Cell counts were performed automatically using the confluence estimation method using a Cytation-5 multi-mode plate reader (BioTek), doubling time was estimated as the number of hours cells took to grow from ∼20% to ∼40% confluency.
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bioRxiv - Synthetic Biology 2020Quote: ... the luminescence signals were quantified by using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, Winooski, VT, USA).
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bioRxiv - Microbiology 2020Quote: ... Plates were imaged with both bright field and red channel RFP using a Cytation 5 plate reader (BioTek, VT). Images were analyzed for worm survival using CellProfiler ...
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bioRxiv - Microbiology 2022Quote: ... Luminescence was quantified with an integration time of 1s using the Cytation 5 Cell Imaging Multi-Mode Reader (BioTek).
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bioRxiv - Cancer Biology 2022Quote: ... The UV absorbance was then read on a Cytation-5 plate reader at 405 nm (BioTek, Winooski, VT, USA). Fraction affected was calculated as follows:
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bioRxiv - Developmental Biology 2022Quote: Images of cultured gastruloids and CFC plates were captured using the Cytation 5 Cell Imaging Multi-Mode Reader (Biotek) plate reader using bright field and FITC channels ...
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bioRxiv - Microbiology 2022Quote: ... Fluorescence was recorded for 5 mins at 1 min intervals at 37°C (BioTek Neo; Ex. 494nm; Em. 520nm). After 5 min ...
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bioRxiv - Bioengineering 2022Quote: ... RNA concentration was measured for both isolated RNA aliquots using a Cytation 5 Multi-Mode Reader (Agilent Biotek Instruments) with a Take3 Micro-Volume Plate at the wavelengths of 260 ...
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bioRxiv - Cancer Biology 2024Quote: ... GFP images were taken 20 hrs post infection and fluorescence measured by the Cytation 5 Imaging Plate Reader (BioTek). Viral replication was measured from supernatants collected 24 hrs post infection and viability determined at 48 hrs by 3D CellTiter-Glo assay following the manufacturer’s instructions (Promega).
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bioRxiv - Bioengineering 2024Quote: ... Primary Cells were stained with DAPI and then imaged with a high throughput imaging system (BioTek Cytation 5, Agilent).
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bioRxiv - Immunology 2023Quote: ... The luminescence from the wells was measured using Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments, VT, USA). The luminescence from blank wells containing 120 µL DMEM with 10% fetal bovine serum and 50 µL CellTitre Glo reagent was recorded as baseline values ...
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bioRxiv - Cell Biology 2023Quote: ... 5 mM d-glucose) twice before a 10 minute incubation in a 37°C plate reader (Synergy H4, BioTek), optionally with DMSO or Dyngo4a (30 μM ...
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bioRxiv - Immunology 2023Quote: ... Plates were incubated for 10 min at room temperature and luminescence was then acquired on a Cytation 5 (BioTek) with an integration time of 0.2 seconds.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The microplate was then incubated and allowed to equilibrate at 37°C in a microplate reader (BioTek Cytation 5) while kinetic fluorescence readings (ex./em. ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then rinsed with PBS containing 1% FBS prior to imaging using a Cytation 5 instrument (Agilent BioTek). Mean fluorescence was calculated using Gen5 software (RRID:SCR_017317).
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bioRxiv - Microbiology 2024Quote: ... Bacterial growth was measured every 30 min for 18 h using a Cytation 5 cell imaging multimode reader (BioTek).
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bioRxiv - Cancer Biology 2021Quote: ... was added to each well for 3 h and the absorbance at 450 nm was measured on an Epoch™ Microplate Spectrophotometer (Biotek, Winooski, VT, USA). Cytotoxicity data were expressed as mean ± standard derivation (SD ...
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bioRxiv - Cell Biology 2022Quote: ... 25 μL of xanthine 1x and the absorbance was read at 450 nm in a BioTek Cytation 3™ multiplate reader (BioTek Instruments Inc., USA). Results were expressed as % of the pool of controls.
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bioRxiv - Cell Biology 2024Quote: ... 100 µL of Tris (pH 10) was added and optical density was measured at 540 nm in Biotek Cytation 3 reader (Biotek Instruments, Winooski, VT, USA) and analyzed using the Wave Desktop 2.6.1 software (RRID:SCR_014526).
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bioRxiv - Bioengineering 2023Quote: ... We affixed the balloons to a glass microscope slide using tape and acquired images at 4x magnification with a Cytation 3 Image reader (BioTek Instruments Inc, Winooski, VT). All images were exported using a 16-bit resolution (65536 grey levels ...
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bioRxiv - Neuroscience 2021Quote: ... Uptake of MEs labeled with FM 4-64 and RNA cargos labeled with ExoGlow-RNATM was quantified by using a microplate fluorescence reader (BioTek Instruments, Inc.) and confocal microscopy imaging respectively ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 11.1 μl of Alamar blue reagent was added and the readings were taken after 4 hours in a Synergy H1 plate reader (BioTek, Winooski, VT) with 560 nm (excitation ...
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bioRxiv - Cell Biology 2022Quote: ... and changes in intracellular Ca2+ concentrations were measured by recording the ratio of fluorescence intensities at 508/20 nm (15 second time interval) using a Synergy 4 multimode microplate reader (BioTek, Winooski, VT). Traces were normalized to their respective baseline intracellular Ca2+ level obtained by averaging the 340/11 nm/380/20 nm ratios of each experiment in the absence of stimulus ...
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bioRxiv - Microbiology 2022Quote: ... The plate was incubated with shaking at 37 °C and the optical density of each well was measured at a wavelength of 600 nm (BIOTEK, Synergy 4). The MIC values were taken as the lowest concentration for which no growth was discernible (<0.05 OD600 ...
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bioRxiv - Microbiology 2024Quote: ... NBT was reduced to blueformazan and spectrophotometrically read at OD 650 nm using a Synergy 4 microplate reader (BioTek, Santa Clara, USA). The concentration at which the drugs were able to inhibit 50% parasite growth (IC50 ...
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bioRxiv - Microbiology 2023Quote: ... The resulting 1,472 SICs were arranged in 16 96-well plates and incubated in the anaerobic chamber at 37 °C (by setting the temperature of the chamber to 37 °C) in a plate stacker (BioTek BioStack 4) with 2 control plates containing uninoculated media at the top and bottom of the stack to minimize edge effects ...
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bioRxiv - Neuroscience 2023Quote: ... After 40 hours, cells were fixed in 4% PFA and images (eGFP, mCherry and eBFP2) were acquired at cytationC10 (Biotek, Objective 60x). eGFP (interaction signal ...