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Citations for Biotek :
651 - 700 of 817 citations for 1 2 Chloropyridin 3 yl 3 3 dimethylazetidin 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... absorbance was measured for each well at 435 nm and 545 nm on a Synergy 2 plate reader (Biotek Instruments, Winooski, VT, USA). Because of variation in background absorbance from well to well on each plate ...
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bioRxiv - Genetics 2020Quote: ... Luciferase activity was determined by analyzing 50 μl of each cell extract in a luciferase assay system according to the standard protocol for the Synergy 2 Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA).
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bioRxiv - Immunology 2023Quote: ... AP-conjugated secondary reagents were detected using Lumi-Phos (Lumigen, Southfield, Michigan, USA; 33% solution in water) and a Synergy 2 Luminometer (BioTek, Winooski, Vermont, USA). Washing steps were performed on an ELx405 Select Deep Well Microplate Washer (BioTek ...
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bioRxiv - Biochemistry 2023Quote: ... The plates were incubated in the multi-well plate reader instruments (Tecan – Spark/infinite, Crailsheim, Germany or BioTek Epoch 2 - BioTek, Bad Friedrichshall, Germany) at 30 °C ...
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bioRxiv - Physiology 2024Quote: ... Cells were then loaded into individual wells of a white 96-well luminescence plate with an automatic injector unit and luminescence was measured for 20 seconds using a Synergy 2 Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA). Each well of the 96-well plate was pre-loaded with candidate ligands (recombinant ITPa ...
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bioRxiv - Zoology 2024Quote: ... and the luminescent response was measured for 20 seconds followed by additional measurements for 10 seconds after ATP injection to the same well with a Synergy 2 Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA).
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bioRxiv - Evolutionary Biology 2022Quote: ... The OD600 was measured every 2 hours for 24 hours at 37°C and slow continuous shaking using a plate reader (Synergy HT BioTek, Winooski, VT, USA). We used population growth rate and yield as fitness measures (Chavhan et al. ...
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bioRxiv - Synthetic Biology 2022Quote: ... of each well every ∼10 minutes with intermittent orbital and linear shaking in a BioTek Epoch 2 plate reader (BioTek, Bad Friedrichshall, Germany). As had previously been established empirically for the instrument ...
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bioRxiv - Biophysics 2022Quote: ... The amount of inorganic phosphate (Pi) at each time point was determined by taking the absorbance at 620 nm using a plate reader (BioTek Synergy Neo 2) and comparing to a standard Pi concentration plot.
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bioRxiv - Plant Biology 2024Quote: Total chlorophyll was extracted from 20 mg frozen powder with 2 ml methanol and quantified spectrophotometrically at 652 nm and 665 nm with a Synergy HTX microplate reader (BioTek, Bad Friedrichshall, DE). Chlorophyll content was calculated using the equation ...
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bioRxiv - Microbiology 2024Quote: ... FITC concentrations on the basolateral side were quantified by measuring the fluorescence intensity (485 nm excitation and 530 nm emission) in a microplate reader (Synergy-2, BioTek, Winooski, VT, USA). FITC levels were calculated using a standard curve of FITC in DMEM with the indicated supplements ...
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bioRxiv - Microbiology 2024Quote: ... removal by vegetative cells was measured by sampling supernatant and assaying AcA using the change in absorbance at 380 nm in the presence of 2--amino benzamidoxime (ABAO), (as inspired by aldehyde quantifications by Ressmann et. al, 2019) using an Epoch microplate reader (BioTek; Gen5 v3.10 software) [42] ...
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bioRxiv - Physiology 2024Quote: ... The increase in absorbance at 412 nm was monitored over time using a microplate reader (PowerWave XS 2, BioTek Instruments, Winooski, VT, USA). This increase in absorbance indicates the formation of thionitrobenzoic acid ...
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bioRxiv - Molecular Biology 2023Quote: ... Absorbance at 450 nm was read with Gen5 All-in-One Microplate Reader (BioTek). ELISA data were analyzed with the software Gen5 V2 Data Analysis Software.
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bioRxiv - Microbiology 2021Quote: ... flat-bottomed 96-well plate and the optical density values were read at 726nm on an Epoch 2 BioTek plate reader (BioTek Instruments, Winooski, VT, USA). The standard curve equation was used to determine total polyphenol content of each sample based on absorbance values.
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bioRxiv - Neuroscience 2020Quote: ... contents were mixed on an orbital shaker for 2 minutes and luminescence readings from experimental samples and ATP standards were acquired using a Synergy H1 microplate reader (BioTek® Instruments, Inc., 253147) and Gen5 Software ...
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bioRxiv - Cell Biology 2022Quote: ... Samples from basolateral chambers were collected at 1 and 2 hours after the start of the experiment and signals from the samples were read by a fluorimeter (BioTek Instruments, Winooski, VT, USA) to determine the amount of dextran passing through the epithelial monolayers.
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bioRxiv - Physiology 2022Quote: ... All assays were run in duplicate under control conditions or in the presence of 5 mM DTT in a 96-well plate and changes in absorbance were tracked using a microplate reader (Synergy 2, BioTek/Agilent, Santa Clara, CA). This concentration was selected based on similar experiments in a previous study [15] ...
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bioRxiv - Physiology 2020Quote: ... The absorbance change vs time for each biomarker was measured spectrophotometrically at a specific wavelength using a spectrophotometer (Epoch 2 microplate spectrophotometer, BioTek Instruments, Inc, Vermont, USA).
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bioRxiv - Biochemistry 2023Quote: ... 250 μl ABTS was mixed with 10 μl of the sample (ethanol and GD extracts) and measured at the wavelength 724 nm using UV/Vis microplate reader (Epoch 2 Microplate Spectrophotometer, BioTek Instruments, Winooski, Vermont, USA). after 15 min of incubation in RT ...
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bioRxiv - Biochemistry 2023Quote: TPC analyses were carried out with the protocol of Singleton and Rossi (Singleton & Rossi, 1965) adopted for microplate reader (Epoch 2 Microplate Spectrophotometer, BioTek Instruments, Winooski, Vermont, USA). Ten microliters of extract ...
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bioRxiv - Microbiology 2023Quote: ... Bacteria were then grown for 24 h at 30°C under continuous shaking in an Epoch 2 Microplate Spectrophotometer from BioTek (Santa Clara, CA, USA). The absorbance at O.D.660 nm was measured every 10 min.
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bioRxiv - Molecular Biology 2024Quote: ... 2-4 images at the central callus and distal callus regions were taken using an automatic microscope (Agilent, BioTek Lionheart FX, Santa Clara, CA). Central callus regions were defined as proximal to the fracture site on either side of the bone ...
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bioRxiv - Cell Biology 2023Quote: ... after which the formazan crystals formed were dissolved using DMSO and the absorbance of the resulting solution at 570 nm measured on a microplate spectrophotometer (Epoch 2, BioTek company, Gen5 software, USA).
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bioRxiv - Microbiology 2023Quote: ... prior to reading under OD885 against the standard solutions of potassium dihydrogen phosphate (0-2 mg/L) using a microplate reader (EPOCH2, Biotek, Santa Clara, California, USA).
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bioRxiv - Microbiology 2023Quote: Optical density of the microplates is measured at 600 nm (OD600) over 72 hours at an interval of 30 mins using Epoch 2 Microplate Spectrophotometer (BioTek Instruments, Inc., Winooski, VT) with the Corning® 96-well clear flat bottom polystyrene microplate #3370 ...
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bioRxiv - Bioengineering 2024Quote: ... This mixture was incubated at 37°C for 2 h and the absorbance was measured at 562 nm using a SYNERGY HTX multi-mode reader (BioTek Instruments Inc., Winooski, VT).
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bioRxiv - Microbiology 2024Quote: ... The extracted RNA was re-suspended in 30 μl RNase-free water then quantified with a BioTek Synergy™2 Multi-detection Microplate Reader (BioTek Instruments, Winooski, VT) and agarose gel electrophoresis ...
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bioRxiv - Synthetic Biology 2024Quote: ... Quantification of red (mScarlet) fluorescence was performed in a 96-well plate format in an automated Synergy 2 plate reader (BioTek Instruments; Winooski, VT, USA) [53–55] ...
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bioRxiv - Microbiology 2024Quote: ... Biolog Phenotypic Microarray plates (Biolog Inc., Hayward, CA) were inoculated and incubated at 37 °C in a stationary incubator and A490 recorded (BioTek Synergy 2, Winooski, VT, USA). End point readings for carbon sources (PM1 and PM2 plates ...
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bioRxiv - Cell Biology 2020Quote: Total protein levels were calibrated using Bicinchoninic Acid (BCA) assay (ThermoFisher Pierce BCA Protein Assay kit, OD measured with BioTek synergy 2 multi-mode multiplate reader). Samples were ran on a 9% SDS polyacrylamide gel ...
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bioRxiv - Microbiology 2020Quote: Pure cultures of defined mutants were diluted to A600 0.003 and grown +/− antibiotic in 96-well microtiter format at 37°C with shaking in a plate reader (Tecan M200 Pro, Biotek Epoch 2, or Biotek Synergy H2M). Growth was monitored as change in A600 ...
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bioRxiv - Biochemistry 2023Quote: ... Representative images are taken at 20x magnification using the Y5 filter cube set of the Cytation-One (Biotek). Representative images are cropped from their original size to emphasize only a few cells per field of view ...
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bioRxiv - Biochemistry 2021Quote: ... Measurements were taken at 340 nm based on the decrease of NADH absorbance over the course of one hour on SYNERGY H1 microplate reader (BioTek). Statistical analysis was performed using GraphPad Prism 8 ...
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bioRxiv - Cell Biology 2023Quote: ... One measure of fluorescence (Ex/Em 560/590 nm) was then read each hour (h) using a Cytation 5 (BioTek) driven by the Gene 5.1.11 Software ...
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bioRxiv - Zoology 2020Quote: ... we extracted DNA from the well-preserved core of one fecal pellet per sample (Menke, Meier, & Sommer, 2015) using the E.Z.N.A.® DNA isolation kit (Omega Biotek, Norcross, GA, U.S.). The DNA concentration and quality were evaluated using Nanodrop™ 2000 Spectrophotometer (Nanodrop ...
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bioRxiv - Cell Biology 2024Quote: ... 90 µl of ATP reagent was added to each well and ATP-dependent bioluminescence (RLU1) was measured after one minute as relative light units (RLU) by Synergy H1 plate reader (BioTek, Agilent). Two wells without cells were used as a blank (RLU0) ...
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bioRxiv - Immunology 2024Quote: ... 24 h post-dsRNA transfection cells were treated with Pierce™ Firefly Luc One-Step Glow Assay Kit and luminescence of firefly luciferase was measured in Synergy H1 (BioTek) microplate reader.
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bioRxiv - Bioengineering 2024Quote: ... and absorbance at 492 nm was monitored in one-minute kinetic interval cycles for 30 minutes using a plate reader (Biotek Cytation 5). The experiment was repeated for the samples after fabrication and biweekly upon storage in physiological conditions ...
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bioRxiv - Synthetic Biology 2022Quote: ... with Cytation 1 (BioTek), or visualized with a blue light or UV transilluminator ...
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bioRxiv - Evolutionary Biology 2023Quote: ... were determined under burst and steady-state conditions using ceftazidime (Δξ = −9,000 M−1 cm−1) at 260 nm by measuring the initial enzymatic reaction rate in a Epoch plate-reader (Biotek). Burst phase rates were determined at 4°C and steady-state parameters were determined at 25°C ...
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bioRxiv - Microbiology 2024Quote: Enzymatic activity of AldA was measured by continuously monitoring the spectrophotometric absorbance changes from NAD to NADH (ε340 nm = 6220 M−1 cm−1) at A340nm using an EPOCH2 microplate spectrophotometer (BioTek). Substrate screening experiments were conducted at 25°C with 1 mM of NAD+ and 5 mM of each aldehyde (i.e. ...
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bioRxiv - Genetics 2020Quote: ... 1 µl of OB Protease Solution (Omega BioTek) and 0.2 µl of RNAse A (100 mg/ml) ...
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bioRxiv - Cancer Biology 2024Quote: ... the plates were loaded on CytationTM 1 (BioTek) for cell counting at the GFP channel ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA Kit 1 (Omega BioTek, Norcross, GA). In conditions where we wanted to observe the effects in myotubes ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... a cell imaging multimode reader (BioTek Cytation 1, Agilent BioTek) and an inverted microscope (DMi8 ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... a cell imaging multimode reader (BioTek Cytation 1, Agilent BioTek) and an inverted microscope (DMi8 ...
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bioRxiv - Microbiology 2022Quote: ... Plates were imaged on a Cytation 1 Multimode Plate Reader (BioTek) using a 4X objective lens ...
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bioRxiv - Immunology 2023Quote: ... Cells were imaged using an automated microscope (Cytation 1, Agilent-BioTek). Infected and total cells were counted using a customized pipeline in CellProfiler (Broad Institute ...
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bioRxiv - Microbiology 2021Quote: ... Automated bright field imaging was conducted using a Cytation 1 reader (Biotek) fitted with high contrast bright field imaging capability controlled by Gen5 software (Biotek) ...