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Citations for Biotek :
501 - 550 of 642 citations for 6 Chlorotetrazolo 1 5 b pyridazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... RNA concentration and quality were measured using a biophotometer and on multimode spot reader using a Cytation™ 5 (BioTek Inc. Winooski, VT). Total RNA from different samples were used to synthesize cDNA using Superscript II reverse transcription kit (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... The neurons were transferred to an automated live-cell incubator/imaging system (BioSpa™ 8 Automated Incubator coupled with a Cytation™ 5 Cell Imaging Multi-Mode Reader, BioTek, USA). The plates were incubated in the BioSpa at 37°C and 5% CO2 for 4 days ...
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bioRxiv - Cancer Biology 2020Quote: ... The plates were then incubated for 1 hour at 37 °C and 5% CO2 and then absorbance at 450 nm was measured using a Synergy H1 Hybrid Multi-Mode Microplate Reader (Biotek, Winooski, VT, USA).
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bioRxiv - Cell Biology 2020Quote: ... neurons were stored in an automated incubator/imaging system (Cytation™ 5 Cell Imaging Multi-Mode Reader associated with a BioSpa™ 8 Automated Incubator, BioTek, USA). The plates were stored in the BioSpa at 37°C and 5% CO2 and were automatically transferred to the Cytation™ 5 for imaging ...
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bioRxiv - Cell Biology 2021Quote: ... isopropyl alcohol to quantitatively measure the density of the stain in different groups at 520 nm using microplate reader (Gene 5 Bio Tek Spectrometer, BioTek, northern Vermont, USA). Isopropanol was used as a blank (26)
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bioRxiv - Neuroscience 2020Quote: ... Immunofluorescence images were acquired using a Cytation 5 Cell Imaging Multi-Mode Reader and analyzed with Gen5™ software (BioTek, Winooski, VT, USA).
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bioRxiv - Microbiology 2021Quote: ... the cells were collected by centrifugation at 10,000 g for 5 min and the absorbance of the supernatant was measured at 562 nm with a Synergy 2 spectrophotometer (BioTek, Winooski, VT, USA). Quantities of ferrihydrite reduced were determined using a standard curve containing a 2-fold range of FeSO4 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... was measured every 5 min for 2 h at 25°C using Synergy H1 Hybrid Multi-Mode Microplate Reader (BioTek, cat. no. H1MFDG). The rate of kinetic reaction (slope ...
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bioRxiv - Cancer Biology 2022Quote: ... The fraction of cells surviving was quantified using either a MacsQuant Analyzer 10 (Milltenyi Biotec, Germany) or by the Cytation 5 system (BioTek, Santa Clara, California). Cell viability was measured according to the manufacturer’s instructions for both instruments ...
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bioRxiv - Biophysics 2022Quote: ... 100 μL of CellTiter-Glo 2.0 was added to each well and luminescence signal was measured in a Cytation 5 imaging reader (BioTek instruments Inc., Winooske, USA) after 10 min incubation.
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bioRxiv - Neuroscience 2023Quote: ... and protein concentrations were determined using the Bradford Assay and an Epoch BioTek Microplate Spectrophotometer (Gen 5 2.01; BioTek Instruments Inc; Winooski, VT). 25 μg of protein from tissue homogenates were combined with specific cytokine antibody-immobilized beads ...
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bioRxiv - Bioengineering 2023Quote: ... The luminescence signal which represents the amount of ATP in viable cells was measured with a microtiter well plate reader (BioTek Cytation 5, Agilent).
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bioRxiv - Bioengineering 2023Quote: ... The fluorescence emission of each well was measured at 593 nm (20 nm bandwidth) with an excitation at 554 nm (18 nm bandwidth) using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments SAS, France). Each condition was replicated three times ...
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bioRxiv - Microbiology 2023Quote: ... ODs were obtained by measuring absorbance at 600nM in a 96-well plate using Cytation Station 5 (BioTek Agilent Technologies, Santa Clara, CA). 108 colony-forming units (CFUs ...
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bioRxiv - Cancer Biology 2023Quote: Fluorescence intensity and absorbance measurements were carried out with Synergy NEO or Cytation 5 multi-mode readers (both from BioTek; Winooski, VT, USA). Bright-field and fluorescence microscopy was carried out with Cytation 5 multi-mode reader using 4× air objective (1.613 µm/pixel ...
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bioRxiv - Cancer Biology 2023Quote: ... was supplied by gas controller into the incubator of Cytation 5 multi-mode reader system equilibrated at 37 °C (BioTek; Winooski, VT, USA).
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bioRxiv - Microbiology 2023Quote: ... ODs were obtained by measuring absorbance at 600nM in a 96-well plate using Cytation Station 5 (BioTek Agilent Technologies, Santa Clara, CA). The liquid cultures were centrifuged at 3500 rpm for 5 minutes (Sorvall Legend X1R M20 rotor ...
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bioRxiv - Cell Biology 2024Quote: ... 2.5 mM calcium chloride for 15 min at room temperature prior to recording fluorescence on a Citation 5 microplate reader (Agilent-BioTek, Santa Clara, CA), exciting at 488 nm and measuring emission at 535 nm ...
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bioRxiv - Cell Biology 2024Quote: ... 2.5 mM calcium chloride for 15 mins at RT prior to examining fluorescence on a Citation 5 microplate reader (Agilent-BioTek, Santa Clara, CA), exciting at 535 nm and measuring emission at 610 nm ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated for 24 h at 37 °C in the presence of 5% CO2.with an automated liquid dispenser (BioTek MicroFlo; Thermo-Fisher, Inc.) Drugs/chemicals were dosed in DMSO at 12 concentrations ranging from 50 pM to 50 μM (half-log dilutions ...
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bioRxiv - Microbiology 2023Quote: ... The fluorescence intensity of FM4-64 was measured at 515/20 nm excitation and 635/20 nm emission wavelength using Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, Winooski, VT, United States). A non-coated plate was used with EVs as a control.
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bioRxiv - Microbiology 2023Quote: ... All samples were mixed via pipetting before fluorescence was read at 490ex/535em and 440ex/535em after 5 minutes using a spectrophotometer (Biotek SynergyMx with Gen5 software). Background fluorescence from buffer alone was subtracted from each sample before calculating the ratiometric fluorescence of the calibration curve samples and experimental samples ...
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bioRxiv - Bioengineering 2024Quote: ... DMMB solution was added (200 µL/well) and absorbance was measured at 540 nm and 590 nm using a plate reader (BioTek Cytation 5, Agilent Technologies). For all samples ...
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bioRxiv - Bioengineering 2024Quote: ... Fluorescence was quantified using an excitation of 530/15 nm and emission of 590/15 nm on a fluorescent plate reader (BioTek Cytation 5, Agilent Technologies).
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bioRxiv - Bioengineering 2020Quote: ... 5% CO2 using an automated digital microscope with a 10× objective with a gas controller (Cytation 3 microplate reader, BioTek Instruments Inc., Winooski, VT, USA). Images were collected every 10 minutes for 24h ...
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bioRxiv - Synthetic Biology 2023Quote: ... was measured by exciting the samples with polarized light at 485/20 nm and emission at 528/20 nm at a parallel and perpendicular angle for 40 min with an interval of 5 min to make sure the plateau is maintained using a Synergy 4 plate reader (BioTek Synergy 4, Vermont, United States). Fluorescence polarization was then calculated using the following equation: Fǁ = fluorescence parallel to the excitation plane ...
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bioRxiv - Bioengineering 2023Quote: ... Doxorubicin fluorescence (480 nm excitation, 590 nm emission peak) was quantified with the Cytation 5 manager plate reader (BioTek® Instruments, Inc., Winooski, VT, USA). The doxorubicin standard curve was prepared by quantifying the fluorescence of a serial dilution in PBS from 1.0 × 103 to 1.2 × 10−4 µM ...
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bioRxiv - Evolutionary Biology 2023Quote: ... were determined under burst and steady-state conditions using ceftazidime (Δξ = −9,000 M−1 cm−1) at 260 nm by measuring the initial enzymatic reaction rate in a Epoch plate-reader (Biotek). Burst phase rates were determined at 4°C and steady-state parameters were determined at 25°C ...
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bioRxiv - Microbiology 2024Quote: Enzymatic activity of AldA was measured by continuously monitoring the spectrophotometric absorbance changes from NAD to NADH (ε340 nm = 6220 M−1 cm−1) at A340nm using an EPOCH2 microplate spectrophotometer (BioTek). Substrate screening experiments were conducted at 25°C with 1 mM of NAD+ and 5 mM of each aldehyde (i.e. ...
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bioRxiv - Genetics 2020Quote: ... 1 µl of OB Protease Solution (Omega BioTek) and 0.2 µl of RNAse A (100 mg/ml) ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... a cell imaging multimode reader (BioTek Cytation 1, Agilent BioTek) and an inverted microscope (DMi8 ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... a cell imaging multimode reader (BioTek Cytation 1, Agilent BioTek) and an inverted microscope (DMi8 ...
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bioRxiv - Microbiology 2022Quote: ... Plates were imaged on a Cytation 1 Multimode Plate Reader (BioTek) using a 4X objective lens ...
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bioRxiv - Immunology 2023Quote: ... Cells were imaged using an automated microscope (Cytation 1, Agilent-BioTek). Infected and total cells were counted using a customized pipeline in CellProfiler (Broad Institute ...
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bioRxiv - Microbiology 2021Quote: ... Automated bright field imaging was conducted using a Cytation 1 reader (Biotek) fitted with high contrast bright field imaging capability controlled by Gen5 software (Biotek) ...
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bioRxiv - Microbiology 2021Quote: ... Collection of data was done using the Cytation 1 (BioTek, United States). The OD600 absorbance of all bacterial cultures was measured every 30 min for 24h at a temperature of 37°C and agitation of 355 cpm (cycles per minute) ...
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bioRxiv - Molecular Biology 2022Quote: ... The Luciferase signal was measured using the plate reader (Cytation 1, Biotek).
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bioRxiv - Immunology 2023Quote: ... Absorption was measured at 405nm on a Cytation 1 plate reader (BioTek). Data was analyzed and plotted with Prism version 10.0.0 (Graphpad).
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bioRxiv - Synthetic Biology 2023Quote: ... for incubation in a Cytation 1 multi-mode plate reader (Agilent BioTek). Plate lids were treated with 10% Triton X-100 in ethanol to prevent fogging (73) ...
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bioRxiv - Microbiology 2020Quote: ... samples were then processed using the BioTek Cytation 1 Imaging Plate Reader (BioTek).
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bioRxiv - Microbiology 2022Quote: ... Plates were imaged using a Cytation 1 multimode plate reader (Biotek, Winooski, VT). Multiple non-overlapping images were taken for each well to ensure at least 5,000 cells were captured ...
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bioRxiv - Genomics 2023Quote: ... cells were imaged using Cytation™1 Cell Imaging Multi-Mode Reader (BioTek). Green fluorescent channel was used for GFP ...
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bioRxiv - Cell Biology 2020Quote: ... The intensity of the purple color was measured using cytation 1 plate reader (BioTek) at 570 nm ...
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TSG101 Associates with PARP1 and is Essential for PARylation and DNA Damage-induced NF-κB ActivationbioRxiv - Molecular Biology 2021Quote: ... Crystal violet intensities were measured at 570 nm with a plate reader (Cytation 1, Biotek).
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bioRxiv - Bioengineering 2020Quote: ... luminescence was read immediately using a plate reader with a 1 second integration time (BioTek Synergy HT Plate Reader and Gen5 Software ...
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bioRxiv - Cell Biology 2019Quote: ... and unbound ZO-1 construct fluorescence intensity was analyzed using a fluorimeter (Biotek Instruments, Inc.). Bound fractions were fitted with the following equation ...
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bioRxiv - Genomics 2019Quote: ... The attached monocyte numbers were evaluated on Cytation™ 1 Cell Imaging Multi-Mode Reader (BioTek) using green fluorescent channel ...
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bioRxiv - Genetics 2020Quote: ... and samples were imaged on a Cytation 1 Cell Imaging Multi-Mode Reader (BioTek, Winooski, VT). Images were evaluated using CellProfiler66 and infection efficiencies were calculated from the percentage of virus mRNA positive-cells in each sample ...
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bioRxiv - Genomics 2023Quote: ... The attached monocyte numbers were quantified on Cytation™1 Cell Imaging Multi-Mode Reader (BioTek) using green fluorescent channel ...
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bioRxiv - Synthetic Biology 2019Quote: ... 1 mg/mL = 39.38 μM).23 Measurements were carried out on Synergy H1 and Neo2 (Biotek Instruments) plate readers ...