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Citations for Biotek :
451 - 500 of 548 citations for n4 Benzoyl 5 methyldeoxycytidine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Fluorescence intensities of the acidic solutions were measured at an excitation/emission of 443/505 nm on A Cytation 5 multimode plate reader (BioTek, Inc). Next ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... and infected cells were maintained at 37°C for 14 to 16 h post infection before manual counting of eGFP+ and mNG+ cells or automated counting using a Cytation 5 cell imaging multi-mode reader (BioTek Instruments) and a CellInsight CX5 imager (Thermo Fisher ...
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bioRxiv - Bioengineering 2022Quote: Luminescence was measured on a plate reader (BioTek Synergy™ HTX for collecting data presented in Figures 1, 3, 5, S5, S8, S12, S14; BioTek Synergy™ Neo2 for Figures 2C-D ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with 2.5μM CellROX for 30min at 37°C after which culture media was replaced for imaging on a BioTek Cytation 5 imaging reader (BioTek, VT). Images were captured from 6 random areas of each well every 10min for 2h ...
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bioRxiv - Plant Biology 2021Quote: ... Absorbance of 50 μl aliquots was determined at 648 and 665 nm using a Cytation 5 plate reader (BioTek, Winooski, VT) and content of chlorophyll a and b per μg wet weight determined using the equations in Barnes et al ...
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bioRxiv - Genetics 2022Quote: ... Brightfield and GFP images were taken every 3 hours for 30 hours using the Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments). GFP quantification was performed by a Cell Profiler program (https://cellprofiler.org/) ...
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bioRxiv - Genetics 2022Quote: ... Brightfield and RFP-channel images were taken every 30 minutes for 12 hours using the Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments). Fluorescence quantifications and the counting of fraction of dead worms were performed by a Cell Profiler program ...
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bioRxiv - Cancer Biology 2022Quote: Cancer cell abundance was observed throughout treatment by imaging mono-cultures and co-culture spheroids using a Cytation 5 cell imaging multimode reader (BioTek Instruments) under dual Bright Field and YFP (ex 500 / em 542 for Venus florescence ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were incubated at room temperature for 10 minutes and read using the Cytation 5 96 well plate reader (BioTek, Vermont) and Gen5 software (version 2.07.17 ...
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bioRxiv - Molecular Biology 2021Quote: ... the firefly LUC and Renilla LUC activities were measured by Dual Luciferase Reporter Gene Assay Kit (Yeasen, Shanghai) and Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, USA). Activity is expressed as the ratio of firefly LUC activity to Renilla LUC activity ...
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bioRxiv - Biochemistry 2020Quote: ... Transfer 100 µL of the solubilized crystal violet to another plate and quantify the absorbance at 550 nm using Cytation™ 5 Cell Imaging Multi-Mode Reader from BioTek.
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bioRxiv - Biochemistry 2020Quote: ... The spectra were recorded using Synergy H1 Hybrid Multi-Mode Reader and Cytation™ 5 Cell Imaging Multi-Mode Reader from BioTek and were averaged by 3 scans.
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bioRxiv - Immunology 2020Quote: ... Samples were incubated for 1 hour at 37°C with 5% CO2 and OD440nm & OD650nm were then measured using a 96-well plate spectrophotometer (Molecular Devices or BioTek). OD650nm was subtracted from OD440nm then percent viability was calculated relative to the no treatment condition.
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bioRxiv - Microbiology 2021Quote: ... coli cells suspensions were adjusted to an optical density at 600 nm (OD600; measured using a UV-visible spectrophotometer, Cytation 5, Biotek Inc.) measurement of 1 ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed 5 times with S Basal then liquid was aspirated using an EL 406 Plate washer / Liquid dispenser (BioTek, VT). 50 μL of 0.01% tween was added to each well to limit worms adhering to pipette tips ...
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bioRxiv - Physiology 2022Quote: ... three representative images of the samples were taken with a Cytation 5 cell imaging multi-mode reader with Gen5 software (Version 3.10; Biotek, Vermont, USA) and analyzed using ImageJ ...
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bioRxiv - Physiology 2024Quote: ... live cells stained with Hoechst 33342 (1:2,000 dilution) (ThermoScientific, Cat#62249) were enumerated using Cytation 5 plate reader (BioTek Instruments - Agilent). Values obtained from the Mito-Stress test were normalized per 1,000 cells.
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bioRxiv - Bioengineering 2023Quote: ... the fluorescence of each well was measured at 560/590 nm (excitation/emission) using a microplate reader Cytation 5 (BioTek Instruments). The number of viable cells per well was calculated against a standard curve prepared by plating various concentrations of isolated cells ...
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CRISPR-Cas9 Engineered Extracellular Vesicles for the Treatment of Dominant Progressive Hearing LossbioRxiv - Bioengineering 2023Quote: ... and the resulting emulsion was collected within an Eppendorf tube or the microplate analyzed under the inverted microscope Cytation 5 (BioTek, USA). The isolation of aqueous phase containing RNP-EV from the oil phase was performed under the centrifuge at 2000-3000xg for 5-10min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Plate was read for luminescence end-point kinetics with a 1s integration on a Cytation 5 Plate Reader using Gen5 software (Biotek Instruments). Relative luminescence units for each siRNA condition were normalized to NTC siRNA and displayed as a percentage.
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bioRxiv - Molecular Biology 2022Quote: ... and incubated at 95 °C for 5 min before the absorbance was measured at 540 nm using a microplate spectrophotometer (BioTek HT).
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bioRxiv - Microbiology 2023Quote: ... and measurement of Renilla luciferase activity by adding Stop & Glo® were performed according to the manufacturer instructions using a luminometer reading (Cytation 5 imaging multimode reader, Biotek).
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bioRxiv - Pathology 2024Quote: ... Alexa 488 fluorescence (485/20, 528/20 nm) or Alexa 647 (635/25, 670/25) was measured by the plate reader Cytation 5 (BioTek, USA).
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bioRxiv - Biochemistry 2024Quote: PLpro enzyme titration and initial velocity analyses were performed using a Cytation 5 multi-mode microplate reader (BIOTEK Instruments, Winooski, VT) as described previously 15 ...
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bioRxiv - Biophysics 2024Quote: ... λex 320 nm) for each reaction was recorded every 20 seconds on a Cytation 5 plate reader (Biotek, software Gen v3.04). The fluorescence data over time were fitted to the monoexponential decay using the curve_fit function in Scipy Python package: ...
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bioRxiv - Biochemistry 2024Quote: ... detectable light signals in the 520 to 620 nm range were produced and captured with a Cytation 5 plate reader (BioTek®) with the gain value set at 180 ...
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bioRxiv - Cell Biology 2020Quote: ... values were normalized to the cell count using Hoechst dye in the final prot injection and transferred to the Cytation 5 (BioTek, Winooski, VT).
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bioRxiv - Bioengineering 2020Quote: ... Wells were imaged at time of scratch and 24□h and 48 h later using Gen 5 software (BioTek Instruments, Winooski, VT) on a Cytation 5 plate reader (BioTek Instruments) ...
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bioRxiv - Cell Biology 2022Quote: ... COX activity was determined as the maximal rate of oxidation of fully reduced cytochrome c measured by the change in absorbance over time at 550 nm using an imaging multi-mode reader (BioTek, Cytation 5) at 30 °C ...
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bioRxiv - Microbiology 2020Quote: ... Samples were examined using an Olympus 1×81 spinning disk confocal microscope (Tokyo, Japan) or Cytation 5 Cell Imaging Multi-Mode Reader instrument (Biotek, Winooski, VT). Images were analyzed using Volocity or Gen5 software ...
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bioRxiv - Microbiology 2020Quote: ... The RNA was quantified by spectrophotometry using a TAKE3 plate in a Cytation 5 multi-mode plate reader (Biotek, Winooski, VT, USA), and RNA quality was assessed by analysis of ribosomal RNA bands visualized following gel electrophoresis by SYBR-safe dye incorporation ...
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bioRxiv - Biochemistry 2021Quote: ... Daily cell number was determined by addition of 20 μl of AqueousOne Solution and measurement of absorbance at 490 nm using a Cytation 5 imaging system (BioTek, Winooski, VT). Fold change was calculated by dividing by absorbance measurements taken at day zero.
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bioRxiv - Microbiology 2021Quote: ... the polymerase activity was monitored by the detection of fluorescence reporter expression on a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, Sursee, Switzerland) equipped with a 4x air objective (numerical aperture (NA) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 50 ul of the reaction mixture was added to 200 ul of Stop solution and fluorescence was read using Cytation 5 (Biotek, Winooski, VA) at 360 nm (Excitation ...
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bioRxiv - Microbiology 2021Quote: For the expression level determination of the strains with IPTG‐induced folA‐gfp expression (Fig. 5) we used a combination of plate readers (Biotek Synergy H1) for optical density measurements for growth rate determination (see section growth rate measurements for details ...
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bioRxiv - Microbiology 2021Quote: ... 4 and 5 were performed using two plate readers: A Synergy Neo2 and a Synergy H1 (both from Biotek Inc., VT, USA). Both were set to 30°C with continuous shaking at an orbital displacement of 1 mm and a speed of 807 rpm ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The relative luciferase units (RLUs) in each well were detected using Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, Winooski, VT, USA).
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bioRxiv - Microbiology 2021Quote: ... The growth and lysis kinetics were recorded by measuring the optical density at a wavelength of 600nm (OD600nm) at 37°C with 5-s shaking every 20 min using a 96-well plate reader (Synergy; BioTek, Winooski, VT)
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bioRxiv - Microbiology 2020Quote: ... The reaction was stopped with 3M sulfuric acid 5-10 minutes after OPD addition and absorbance values were measured at a wavelength of 492nm on a spectrophotometer (Biotek Cytation5 system). Absorbance values were normalized by subtracting uncoated wells from antigen-coated wells at the corresponding serum dilution ...
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bioRxiv - Microbiology 2021Quote: ... Quantification was performed by measuring soluble A600 CV absorbance using a Biotek Synergy HT microplate reader and Gen 5 software (Biotek, Winooski, VT). Values were normalized to culture growth by dividing by final culture OD600.
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bioRxiv - Microbiology 2023Quote: ... The reaction was stopped by adding 50 μl of stop solution and the absorbance was recorded at 450nm using Cytation 5 microplate reader (BioTek Instruments, USA).
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bioRxiv - Microbiology 2023Quote: ... Plasmin activity was monitored by continuously measuring the rate of change of absorbance at 405 nm for 20 minutes using Cytation 5 microplate reader (BioTek Instruments, USA).
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bioRxiv - Microbiology 2023Quote: GFP or mCherry-positive cells were imaged on the Cytation 5 imaging reader and masked using the accompanying Gen5 software (BioTek Instruments, Inc.). The final cell-to-cell spread was calculated as GFP or mCherry-positive cells per area of the entire well ...
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bioRxiv - Bioengineering 2023Quote: ... was quantified by measuring the absorption at 620 nm using a UV/Vis-spectrophotometer (Cytation 5 imaging reader: BioTek, Winooski, VT, USA). A standard curve was used to correlate the absorption value with the amount of HA ...
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bioRxiv - Plant Biology 2023Quote: ... The background luminescence of seedling was recorded for 2 min before application of flg22 and chitin (5 s for NaCl) on a microplate reader (BioTek Synergy H1). Then 50 μl of 200 nM flg22 ...
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bioRxiv - Cancer Biology 2022Quote: ... and day 2 by addition of the CTG reagent followed by measurement of luminescence using the Biotek Cytation 5 (BioTek, Winooski, VT). Luminescence values were adjusted based on 2µM Adenosine triphosphate (ATP ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The O.D./min was continuously recorded at 405 nm over 30 min to measure the hydrolytic product 5-mercapto-2-nitrobenzoic acid using a Synergy H1 microplate reader (Biotek; VT, USA). O.D./min was converted to catalytic rate using the Beer-Lambert law:
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bioRxiv - Biochemistry 2022Quote: Cy5.5-ALN was characterized in terms of absorbance in the NIR region using a plate reader (Cytation 5, BioTek, Winooski, VT, USA). Yield was calculated using ε675nm=211000 M/cm ...
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bioRxiv - Developmental Biology 2022Quote: ... Plates were maintained at 5% CO2 and 37°C during imaging using Cytation Gen5 Microplate Reader and Imager Software (BioTek, version 3.08.01). Protocols specific to Falcon 24 well companion plates (Corning ...
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bioRxiv - Plant Biology 2022Quote: ... The DNA was normalized to 5 ng/μL based on quantification with a Synergy 2 multimode microplate reader (Biotek Instruments, Winooski, VT) with the Quant-iT PicoGreen double-stranded DNA quantification assay (Thermo Fisher Scientific ...