Labshake search
Citations for Biotek :
451 - 500 of 514 citations for 6 2 Ethoxyphenyl 6 oxohexanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... The 96-well plates were incubated anaerobically in a temperature-controlled plate reader at 37°C (Epoch 2 Microplate Spectrophotometer, BioTek, Swindon, UK). For each test and technical replicate ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2 h later the fluorescence was read at 340/505 and 380/505 on a Cytation™ 3 (BioTek (Vermont, MA, USA)) multifunctional microplate reader ...
-
bioRxiv - Biochemistry 2020Quote: ... Ten parallel bacterial cultures of 100 μL for each condition were shaken in a Synergy 2 microplate reader (BioTek, Winooski, VT, USA) at 37 oC ...
-
bioRxiv - Bioengineering 2021Quote: ... Then the plate was vortexed at 400 rpm for 2 min and luminescence read on a Synergy Neo2 plate reader (BioTek, Winooski, VT).
-
bioRxiv - Microbiology 2021Quote: ... The OD600 was used to monitor growth during incubation at 30 °C for 48 h without aeration using a Synergy 2 microplate reader (Biotek, Winooski, VT).
-
bioRxiv - Biochemistry 2021Quote: ... and inhibitor (APPI monomer, 0-400 nM; APPI dimer, 0-96 nM) in a Synergy 2 Multi-Detection Microplate Reader (BioTek, VT, USA). Mesotrypsin buffer (296 μL) ...
-
bioRxiv - Biochemistry 2021Quote: ... Fluorescent polarization was read parallel and perpendicular to the excitation plane (ex. 485 nm/ em. 528 nm) using a Synergy 2 Microplate reader (BioTek, Winooski, VT) and the relationship between signal and time was linear (Figure S2) ...
-
bioRxiv - Cancer Biology 2020Quote: ... and the plate was returned to incubator for 2 hours after which absorbance was read at 450 nm in a microplate reader (BioTek Instruments Inc).
-
bioRxiv - Microbiology 2020Quote: ... Serosal samples were collected every 30 minutes for 2 hours with FITC dextran flux (ng/ml/cm2/hr) determined by plate reader (Biotek Synergy H1).
-
bioRxiv - Immunology 2021Quote: ... and light emission was measured every 2 minutes for a period of 120 min using the Synergy H1 microplate reader (Biotek Instruments, USA). The light emission levels are expressed as RLU (Relative Light Units = luminescence measured by the luminometer).
-
bioRxiv - Plant Biology 2023Quote: ... The background luminescence of seedling was recorded for 2 min before application of flg22 and chitin (5 s for NaCl) on a microplate reader (BioTek Synergy H1). Then 50 μl of 200 nM flg22 ...
-
bioRxiv - Immunology 2023Quote: ... This was achieved by collecting samples from the serosal compartments every 30 minutes for 2 hours and measuring the respective FITC concentrations on a plate reader (Synergy H1; BioTek, Winooski, VT). Samples with visible FITC leakage in the serosal compartment were omitted ...
-
bioRxiv - Cancer Biology 2022Quote: ... and day 2 by addition of the CTG reagent followed by measurement of luminescence using the Biotek Cytation 5 (BioTek, Winooski, VT). Luminescence values were adjusted based on 2µM Adenosine triphosphate (ATP ...
-
bioRxiv - Genetics 2023Quote: ... The plates were sealed with clear plastic adhesive film and incubated at 30°C with no shaking in a microplate spectrophotometer (BioTek Epoch 2) with OD600 readings taken of each well every 30 minutes for 48 hours ...
-
bioRxiv - Plant Biology 2022Quote: ... The DNA was normalized to 5 ng/μL based on quantification with a Synergy 2 multimode microplate reader (Biotek Instruments, Winooski, VT) with the Quant-iT PicoGreen double-stranded DNA quantification assay (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... were incubated in a 96-well flat-bottom plate (Nunclon) at 37°C with continuous shaking (double orbital) in a microplate spectrophotometer (BioTek Epoch 2). Optical densities were recorded every 20 min ...
-
bioRxiv - Plant Biology 2022Quote: ... The reaction was followed by measuring the absorbance at 363 nm at 25°C for 15 min using a plate reader (BioTek Synergy 2). For NR activity measurements on material extracted from Arabidopsis ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The absorbance at 405 nm was measured every 1 min for at least 30 min and up to 2 h using a Synergy 4 microplate reader (Biotek, Winooski, VT). SEAP activities were calculated as a function of the increase in absorbance at 405 nm over time42.
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... were added to wells in a microplate and incubated for 2 min at 37°C in a microplate reader (Cytation 3 Imaging Reader, BioTek, VT, USA). Then ...
-
bioRxiv - Microbiology 2024Quote: ... the OD600 and the relative fluorescence units (RFU) of the cultures were recorded hourly using a Synergy 2 Multi-Mode reader (BioTek, Winooski, VT) and the RFU results were normalized against the OD600 of the culture (82).
-
bioRxiv - Microbiology 2024Quote: ... OD660 nm was recorded every 10 min for 24 h at 30 °C under continuous shaking in an Epoch 2 absorbance reader (Biotek Instruments Inc.). When required ...
-
bioRxiv - Neuroscience 2024Quote: ... ELISA plates were read with a Synergy 2 Gen 5 microplate reader at 405 nm absorbance (BioTek Instruments Inc., Winooski, VT, USA).
-
bioRxiv - Cell Biology 2024Quote: ... and the samples’ optical density (OD) was measured in a 96 wells plate at 540 nm using a spectrophotometer (Epoch 2, BioTek, Winooski, USA). ODs were corrected by the total number of cells counted (Countess 3 Cell Counter ...
-
bioRxiv - Cell Biology 2020Quote: ... The purified extracts of CanlonicSF were excited at 485 ± 20 nm and the intensity of fluorescence emission of cpGFP was recorded at 530 ± 15 nm using the multiwell plate analyzer Synergy 2 (Biotek, Winooski, Vermont, USA). Excitation and emission spectra were obtained by collecting the emissions at 515 ± 15 nm using a multiwell plate reader EnVision (PerkinElmer ...
-
bioRxiv - Physiology 2020Quote: ... The absorbance was determined spectrophotometrically at a wavelength of 450 nm using a spectrophotometer (Epoch 2 microplate spectrophotometer, BioTek Instruments, Inc, Vermont, USA).
-
bioRxiv - Biochemistry 2021Quote: We measured the catalytic activity of the enzyme citrate synthase in mitochondrial samples monitoring the conversion of oxaloacetate and acetyl-CoA to citrate and CoA– SH and by measuring the formation of the colorimetric product thionitrobenzoic acid (TNB) at 412 nm and 37°C (Shepherd and Garland, 1969) on a microplate reader (Power Wave XS-2, Biotek Instruments, Winooski, USA). We calculated the enzyme activity using the changes in absorbance after substrate (250 µM oxaloacetate ...
-
bioRxiv - Neuroscience 2020Quote: ... Fluorescence post-thallium addition was monitored every 21 seconds for 600 seconds total using a BioTek Synergy 2 (BioTek, Winooski, VT, United States) multi-well plate reader equipped with an excitation filter of 485/20 nm and emission filter 528/20 nm ...
-
bioRxiv - Microbiology 2022Quote: ... The cells were grown with agitation at 30°C for 30 hours in a microplate reader (Infinite M Plex; Tecan, Austria, or Synergy 2; BioTek, Winooski, VT, USA), and the optical density at 600 nm (OD600 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Aerobic growth was monitored in technical duplicates or triplicates at 37°C in a BioTek Epoch 2 Microplate Spectrophotometer (BioTek, Bad Friedrichshall, Germany) by absorbance measurements (600nm ...
-
bioRxiv - Microbiology 2021Quote: ... the cells were collected by centrifugation at 10,000 g for 5 min and the absorbance of the supernatant was measured at 562 nm with a Synergy 2 spectrophotometer (BioTek, Winooski, VT, USA). Quantities of ferrihydrite reduced were determined using a standard curve containing a 2-fold range of FeSO4 (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2021Quote: ... was grown at 27°C on a 96-well plate (200 μL) and absorbance at 600 mm was recorded on a microplate reader (Synergy 2 Multi-Mode Microplate Reader, BioTek, Winooski, Vermont, USA). Non-inoculated media were used as blank ...
-
bioRxiv - Cell Biology 2021Quote: ... was measured every 5 min for 2 h at 25°C using Synergy H1 Hybrid Multi-Mode Microplate Reader (BioTek, cat. no. H1MFDG). The rate of kinetic reaction (slope ...
-
bioRxiv - Immunology 2020Quote: ... Growth was measured as relative light units at 528 nm after excitation at 485 nm in a fluorescence microplate reader (Synergy 2, BioTek Instruments, Vermont, USA) at the indicated time points.
-
bioRxiv - Genomics 2021Quote: ... absorbance was measured for each well at 435 nm and 545 nm on a Synergy 2 plate reader (Biotek Instruments, Winooski, VT, USA). Because of variation in background absorbance from well to well on each plate ...
-
bioRxiv - Genetics 2020Quote: ... Luciferase activity was determined by analyzing 50 μl of each cell extract in a luciferase assay system according to the standard protocol for the Synergy 2 Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA).
-
bioRxiv - Immunology 2023Quote: ... AP-conjugated secondary reagents were detected using Lumi-Phos (Lumigen, Southfield, Michigan, USA; 33% solution in water) and a Synergy 2 Luminometer (BioTek, Winooski, Vermont, USA). Washing steps were performed on an ELx405 Select Deep Well Microplate Washer (BioTek ...
-
bioRxiv - Biochemistry 2023Quote: ... The plates were incubated in the multi-well plate reader instruments (Tecan – Spark/infinite, Crailsheim, Germany or BioTek Epoch 2 - BioTek, Bad Friedrichshall, Germany) at 30 °C ...
-
bioRxiv - Physiology 2024Quote: ... Cells were then loaded into individual wells of a white 96-well luminescence plate with an automatic injector unit and luminescence was measured for 20 seconds using a Synergy 2 Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA). Each well of the 96-well plate was pre-loaded with candidate ligands (recombinant ITPa ...
-
bioRxiv - Zoology 2024Quote: ... and the luminescent response was measured for 20 seconds followed by additional measurements for 10 seconds after ATP injection to the same well with a Synergy 2 Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA).
-
bioRxiv - Evolutionary Biology 2022Quote: ... The OD600 was measured every 2 hours for 24 hours at 37°C and slow continuous shaking using a plate reader (Synergy HT BioTek, Winooski, VT, USA). We used population growth rate and yield as fitness measures (Chavhan et al. ...
-
bioRxiv - Synthetic Biology 2022Quote: ... of each well every ∼10 minutes with intermittent orbital and linear shaking in a BioTek Epoch 2 plate reader (BioTek, Bad Friedrichshall, Germany). As had previously been established empirically for the instrument ...
-
bioRxiv - Biophysics 2022Quote: ... The amount of inorganic phosphate (Pi) at each time point was determined by taking the absorbance at 620 nm using a plate reader (BioTek Synergy Neo 2) and comparing to a standard Pi concentration plot.
-
bioRxiv - Plant Biology 2024Quote: Total chlorophyll was extracted from 20 mg frozen powder with 2 ml methanol and quantified spectrophotometrically at 652 nm and 665 nm with a Synergy HTX microplate reader (BioTek, Bad Friedrichshall, DE). Chlorophyll content was calculated using the equation ...
-
bioRxiv - Microbiology 2024Quote: ... FITC concentrations on the basolateral side were quantified by measuring the fluorescence intensity (485 nm excitation and 530 nm emission) in a microplate reader (Synergy-2, BioTek, Winooski, VT, USA). FITC levels were calculated using a standard curve of FITC in DMEM with the indicated supplements ...
-
bioRxiv - Microbiology 2024Quote: ... removal by vegetative cells was measured by sampling supernatant and assaying AcA using the change in absorbance at 380 nm in the presence of 2--amino benzamidoxime (ABAO), (as inspired by aldehyde quantifications by Ressmann et. al, 2019) using an Epoch microplate reader (BioTek; Gen5 v3.10 software) [42] ...
-
bioRxiv - Physiology 2024Quote: ... The increase in absorbance at 412 nm was monitored over time using a microplate reader (PowerWave XS 2, BioTek Instruments, Winooski, VT, USA). This increase in absorbance indicates the formation of thionitrobenzoic acid ...
-
bioRxiv - Microbiology 2021Quote: ... flat-bottomed 96-well plate and the optical density values were read at 726nm on an Epoch 2 BioTek plate reader (BioTek Instruments, Winooski, VT, USA). The standard curve equation was used to determine total polyphenol content of each sample based on absorbance values.
-
bioRxiv - Microbiology 2020Quote: ... transferred to 24-well culture plate and the OD600 determined kinetically (1 point/h) using a BioTek Synergy 2 spectrofluorometer (BioTeK U.S., Winooski, VT, USA) at 25°C with continuous slow agitation ...
-
bioRxiv - Neuroscience 2020Quote: ... contents were mixed on an orbital shaker for 2 minutes and luminescence readings from experimental samples and ATP standards were acquired using a Synergy H1 microplate reader (BioTek® Instruments, Inc., 253147) and Gen5 Software ...
-
bioRxiv - Cell Biology 2022Quote: ... Samples from basolateral chambers were collected at 1 and 2 hours after the start of the experiment and signals from the samples were read by a fluorimeter (BioTek Instruments, Winooski, VT, USA) to determine the amount of dextran passing through the epithelial monolayers.