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Citations for Biotek :
451 - 500 of 1217 citations for 2 Amino 6 chloro 9 3 5 di O p toluoyl beta D 2 deoxyribofurnanosyl purine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... Cytation 3 (Biotek, software Gen5 v4.2).
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bioRxiv - Cancer Biology 2021Quote: ... Day0 measurements were taken by acquiring 3×3 images with a 4x objective in Cytation5 (BioTek, USA). Cells were incubated for the subsequent 16 days and images were taken at Day4 ...
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bioRxiv - Cell Biology 2020Quote: ... The purified extracts of CanlonicSF were excited at 485 ± 20 nm and the intensity of fluorescence emission of cpGFP was recorded at 530 ± 15 nm using the multiwell plate analyzer Synergy 2 (Biotek, Winooski, Vermont, USA). Excitation and emission spectra were obtained by collecting the emissions at 515 ± 15 nm using a multiwell plate reader EnVision (PerkinElmer ...
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bioRxiv - Immunology 2022Quote: ... Reactions were stopped by the addition of 2M sulfuric acid and read using a BioTek Synergy 2 or Synergy H1 plate reader set at 450nm (BioTek, Winooski, VT, USA). Receiver operator curve (ROC ...
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bioRxiv - Physiology 2020Quote: ... The absorbance was determined spectrophotometrically at a wavelength of 450 nm using a spectrophotometer (Epoch 2 microplate spectrophotometer, BioTek Instruments, Inc, Vermont, USA).
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bioRxiv - Biochemistry 2021Quote: We measured the catalytic activity of the enzyme citrate synthase in mitochondrial samples monitoring the conversion of oxaloacetate and acetyl-CoA to citrate and CoA– SH and by measuring the formation of the colorimetric product thionitrobenzoic acid (TNB) at 412 nm and 37°C (Shepherd and Garland, 1969) on a microplate reader (Power Wave XS-2, Biotek Instruments, Winooski, USA). We calculated the enzyme activity using the changes in absorbance after substrate (250 µM oxaloacetate ...
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bioRxiv - Neuroscience 2020Quote: ... Fluorescence post-thallium addition was monitored every 21 seconds for 600 seconds total using a BioTek Synergy 2 (BioTek, Winooski, VT, United States) multi-well plate reader equipped with an excitation filter of 485/20 nm and emission filter 528/20 nm ...
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bioRxiv - Microbiology 2022Quote: ... The cells were grown with agitation at 30°C for 30 hours in a microplate reader (Infinite M Plex; Tecan, Austria, or Synergy 2; BioTek, Winooski, VT, USA), and the optical density at 600 nm (OD600 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Aerobic growth was monitored in technical duplicates or triplicates at 37°C in a BioTek Epoch 2 Microplate Spectrophotometer (BioTek, Bad Friedrichshall, Germany) by absorbance measurements (600nm ...
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bioRxiv - Microbiology 2021Quote: ... was grown at 27°C on a 96-well plate (200 μL) and absorbance at 600 mm was recorded on a microplate reader (Synergy 2 Multi-Mode Microplate Reader, BioTek, Winooski, Vermont, USA). Non-inoculated media were used as blank ...
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bioRxiv - Immunology 2020Quote: ... Growth was measured as relative light units at 528 nm after excitation at 485 nm in a fluorescence microplate reader (Synergy 2, BioTek Instruments, Vermont, USA) at the indicated time points.
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bioRxiv - Genomics 2021Quote: ... absorbance was measured for each well at 435 nm and 545 nm on a Synergy 2 plate reader (Biotek Instruments, Winooski, VT, USA). Because of variation in background absorbance from well to well on each plate ...
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bioRxiv - Genetics 2020Quote: ... Luciferase activity was determined by analyzing 50 μl of each cell extract in a luciferase assay system according to the standard protocol for the Synergy 2 Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA).
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bioRxiv - Evolutionary Biology 2022Quote: ... The OD600 was measured every 2 hours for 24 hours at 37°C and slow continuous shaking using a plate reader (Synergy HT BioTek, Winooski, VT, USA). We used population growth rate and yield as fitness measures (Chavhan et al. ...
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bioRxiv - Synthetic Biology 2022Quote: ... of each well every ∼10 minutes with intermittent orbital and linear shaking in a BioTek Epoch 2 plate reader (BioTek, Bad Friedrichshall, Germany). As had previously been established empirically for the instrument ...
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bioRxiv - Physiology 2024Quote: ... Cells were then loaded into individual wells of a white 96-well luminescence plate with an automatic injector unit and luminescence was measured for 20 seconds using a Synergy 2 Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA). Each well of the 96-well plate was pre-loaded with candidate ligands (recombinant ITPa ...
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bioRxiv - Zoology 2024Quote: ... and the luminescent response was measured for 20 seconds followed by additional measurements for 10 seconds after ATP injection to the same well with a Synergy 2 Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA).
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bioRxiv - Immunology 2023Quote: ... AP-conjugated secondary reagents were detected using Lumi-Phos (Lumigen, Southfield, Michigan, USA; 33% solution in water) and a Synergy 2 Luminometer (BioTek, Winooski, Vermont, USA). Washing steps were performed on an ELx405 Select Deep Well Microplate Washer (BioTek ...
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bioRxiv - Biophysics 2022Quote: ... The amount of inorganic phosphate (Pi) at each time point was determined by taking the absorbance at 620 nm using a plate reader (BioTek Synergy Neo 2) and comparing to a standard Pi concentration plot.
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bioRxiv - Biochemistry 2023Quote: ... The plates were incubated in the multi-well plate reader instruments (Tecan – Spark/infinite, Crailsheim, Germany or BioTek Epoch 2 - BioTek, Bad Friedrichshall, Germany) at 30 °C ...
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bioRxiv - Physiology 2024Quote: ... The increase in absorbance at 412 nm was monitored over time using a microplate reader (PowerWave XS 2, BioTek Instruments, Winooski, VT, USA). This increase in absorbance indicates the formation of thionitrobenzoic acid ...
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bioRxiv - Microbiology 2024Quote: ... FITC concentrations on the basolateral side were quantified by measuring the fluorescence intensity (485 nm excitation and 530 nm emission) in a microplate reader (Synergy-2, BioTek, Winooski, VT, USA). FITC levels were calculated using a standard curve of FITC in DMEM with the indicated supplements ...
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bioRxiv - Plant Biology 2024Quote: Total chlorophyll was extracted from 20 mg frozen powder with 2 ml methanol and quantified spectrophotometrically at 652 nm and 665 nm with a Synergy HTX microplate reader (BioTek, Bad Friedrichshall, DE). Chlorophyll content was calculated using the equation ...
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bioRxiv - Bioengineering 2022Quote: ... using Cytation 5 (Biotek). Maximal respiration was calculated by subtracting non-mitochondrial OCR from FCCP OCR ...
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bioRxiv - Molecular Biology 2022Quote: ... using the Cytation 3 plate-reader (BioTek).
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bioRxiv - Microbiology 2021Quote: ... flat-bottomed 96-well plate and the optical density values were read at 726nm on an Epoch 2 BioTek plate reader (BioTek Instruments, Winooski, VT, USA). The standard curve equation was used to determine total polyphenol content of each sample based on absorbance values.
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bioRxiv - Microbiology 2020Quote: ... transferred to 24-well culture plate and the OD600 determined kinetically (1 point/h) using a BioTek Synergy 2 spectrofluorometer (BioTeK U.S., Winooski, VT, USA) at 25°C with continuous slow agitation ...
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bioRxiv - Neuroscience 2020Quote: ... contents were mixed on an orbital shaker for 2 minutes and luminescence readings from experimental samples and ATP standards were acquired using a Synergy H1 microplate reader (BioTek® Instruments, Inc., 253147) and Gen5 Software ...
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bioRxiv - Cell Biology 2022Quote: ... Samples from basolateral chambers were collected at 1 and 2 hours after the start of the experiment and signals from the samples were read by a fluorimeter (BioTek Instruments, Winooski, VT, USA) to determine the amount of dextran passing through the epithelial monolayers.
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bioRxiv - Physiology 2022Quote: ... All assays were run in duplicate under control conditions or in the presence of 5 mM DTT in a 96-well plate and changes in absorbance were tracked using a microplate reader (Synergy 2, BioTek/Agilent, Santa Clara, CA). This concentration was selected based on similar experiments in a previous study [15] ...
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bioRxiv - Physiology 2020Quote: ... The absorbance change vs time for each biomarker was measured spectrophotometrically at a specific wavelength using a spectrophotometer (Epoch 2 microplate spectrophotometer, BioTek Instruments, Inc, Vermont, USA).
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bioRxiv - Microbiology 2023Quote: ... prior to reading under OD885 against the standard solutions of potassium dihydrogen phosphate (0-2 mg/L) using a microplate reader (EPOCH2, Biotek, Santa Clara, California, USA).
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bioRxiv - Molecular Biology 2024Quote: ... 2-4 images at the central callus and distal callus regions were taken using an automatic microscope (Agilent, BioTek Lionheart FX, Santa Clara, CA). Central callus regions were defined as proximal to the fracture site on either side of the bone ...
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bioRxiv - Biochemistry 2023Quote: ... 250 μl ABTS was mixed with 10 μl of the sample (ethanol and GD extracts) and measured at the wavelength 724 nm using UV/Vis microplate reader (Epoch 2 Microplate Spectrophotometer, BioTek Instruments, Winooski, Vermont, USA). after 15 min of incubation in RT ...
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bioRxiv - Biochemistry 2023Quote: TPC analyses were carried out with the protocol of Singleton and Rossi (Singleton & Rossi, 1965) adopted for microplate reader (Epoch 2 Microplate Spectrophotometer, BioTek Instruments, Winooski, Vermont, USA). Ten microliters of extract ...
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bioRxiv - Microbiology 2023Quote: ... Bacteria were then grown for 24 h at 30°C under continuous shaking in an Epoch 2 Microplate Spectrophotometer from BioTek (Santa Clara, CA, USA). The absorbance at O.D.660 nm was measured every 10 min.
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bioRxiv - Microbiology 2023Quote: Optical density of the microplates is measured at 600 nm (OD600) over 72 hours at an interval of 30 mins using Epoch 2 Microplate Spectrophotometer (BioTek Instruments, Inc., Winooski, VT) with the Corning® 96-well clear flat bottom polystyrene microplate #3370 ...
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bioRxiv - Cell Biology 2023Quote: ... after which the formazan crystals formed were dissolved using DMSO and the absorbance of the resulting solution at 570 nm measured on a microplate spectrophotometer (Epoch 2, BioTek company, Gen5 software, USA).
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bioRxiv - Microbiology 2024Quote: ... The extracted RNA was re-suspended in 30 μl RNase-free water then quantified with a BioTek Synergy™2 Multi-detection Microplate Reader (BioTek Instruments, Winooski, VT) and agarose gel electrophoresis ...
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bioRxiv - Bioengineering 2024Quote: ... This mixture was incubated at 37°C for 2 h and the absorbance was measured at 562 nm using a SYNERGY HTX multi-mode reader (BioTek Instruments Inc., Winooski, VT).
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bioRxiv - Synthetic Biology 2024Quote: ... Quantification of red (mScarlet) fluorescence was performed in a 96-well plate format in an automated Synergy 2 plate reader (BioTek Instruments; Winooski, VT, USA) [53–55] ...
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bioRxiv - Molecular Biology 2021Quote: ... The kinetics of substrate cleavage (increase in fluorescence of the liberated 7-amino-4-methylcoumarin, AMC) was measured using a fluorometer (BioTek, USA) set at 370 nm excitation and 460 nm emission at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Biolog Phenotypic Microarray plates (Biolog Inc., Hayward, CA) were inoculated and incubated at 37 °C in a stationary incubator and A490 recorded (BioTek Synergy 2, Winooski, VT, USA). End point readings for carbon sources (PM1 and PM2 plates ...
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bioRxiv - Cancer Biology 2023Quote: ... Plates were imaged using the Cytation 3 (BioTek) and cells were counted using CellProfiler ...
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bioRxiv - Neuroscience 2021Quote: ... diluted in P/S free medium was dispensed using a multi-drop dispenser (MultiFlo FX, Biotek, Vinooski, VT, USA). Plates were centrifuged at 1000xG for 1 minute (Eppendorf 5804R ...
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bioRxiv - Bioengineering 2023Quote: ... Cytation 5 imaging reader (BioTek) was used to read the plate for absorbance at 450nm when the assay was complete.
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bioRxiv - Cell Biology 2020Quote: Total protein levels were calibrated using Bicinchoninic Acid (BCA) assay (ThermoFisher Pierce BCA Protein Assay kit, OD measured with BioTek synergy 2 multi-mode multiplate reader). Samples were ran on a 9% SDS polyacrylamide gel ...
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bioRxiv - Microbiology 2020Quote: Pure cultures of defined mutants were diluted to A600 0.003 and grown +/− antibiotic in 96-well microtiter format at 37°C with shaking in a plate reader (Tecan M200 Pro, Biotek Epoch 2, or Biotek Synergy H2M). Growth was monitored as change in A600 ...
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bioRxiv - Microbiology 2021Quote: ... Imaging was performed using a Cytation 5 multimode imaging system with Gen 5 software (Biotek).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and used to generate sequences with the desired amino acid substitution using Q5 Site-Directed Mutagenesis Kit (NEB, via BioTek, Praha, Czech Republic). Primers were designed using NEBaseChanger (https://nebasechanger.neb.com/) ...