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Citations for R&D Systems :
51 - 100 of 1220 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... as capture antibody and a sulfo-tagged detection antibody (sheep anti-mouse Trem2, AF1729 R&D Systems). MSD plates were developed using 2x MSD read buffer T ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stained 1h at RT with a goat polyclonal anti-human ACE2 (AF933, R&D Systems) diluted at 3μg/ml in PBS + 0.3% triton X-100 + 1% horse serum + 1% BSA + 0,01% sodium azide ...
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bioRxiv - Developmental Biology 2021Quote: ... the wells were plated with 25µl detection antibody (Biotinylated Goat Anti-Mouse IGF-II, R&D Systems – 840963), diluted to 0.72 µg/ml in PBS ...
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bioRxiv - Immunology 2022Quote: ... Detection of IL-13 and IL-4 in lung homogenates was determined using Duoset ELISA (R&D system) as per manufacturers instructions.
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bioRxiv - Immunology 2022Quote: ... The membranes were washed three times with 1× Wash Buffer (R&D Systems) to remove unbound proteins ...
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bioRxiv - Cell Biology 2020Quote: ... and IL-6 (assay detection range 9.38-600 pg/ml) were purchased from R&D Systems (Minneapolis, MN, USA) and were used according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... 100 µL of a solution containing 0.001 mg/mL of the detection biotinylated polyclonal antibody (BAF1559; R&D Systems) raised against NOTCH3 ECD and 2.5% BSA was added and was used for detection followed by agitation for 2h at RT ...
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bioRxiv - Systems Biology 2020Quote: ... Primary detection of hACE-2 was done using a goat anti-hACE-2 antibody (1:1,250; #AF933, R&D Systems), a horseradish peroxidase (HRP)-labeled donkey anti-goat antibody (1:5,000 ...
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bioRxiv - Physiology 2022Quote: ... HRP-tagged clone 351.4.5E1.H3 for detection) and recombinant human FABP4 as a standard (R&D Systems, cat# DY3150-05). For Figure 8C WT mouse plasma FABP4 was measured using a commercially available assay for mouse FABP4 (Mouse Adipocyte FABP (FABP4 ...
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bioRxiv - Physiology 2022Quote: ... HRP-tagged clone 351.4.5E1.H3 for detection) and recombinant human FABP4 as a standard (R&D Systems, cat# DY3150-05). Plasma insulin was assessed using a Crystal Chem Ultra Sensitive Mouse Insulin ELISA ...
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bioRxiv - Cancer Biology 2020Quote: ... Death-related proteins were measured in cell lysates by a solid-phase antibody array with chemiluminescent detection (R&D Systems, 893900).
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bioRxiv - Cell Biology 2019Quote: ... Luminex® Multiplex and ELISA assays for the detection of chemokines and cytokines were purchased from R&D Systems (Minneapolis, MN).
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bioRxiv - Immunology 2020Quote: ... the membrane was incubated for 1 hr at room temperature (RT) with anti-goat HRP conjugated antibody (R&D Systems #HAF109) at 1:2000 in TBST 1% milk ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated for 1 hour at RT with anti-PAR/pADPr monoclonal antibody (R&D Systems cat# 4335-MC-100) diluted 1:250 in 3% BSA-PBS ...
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bioRxiv - Neuroscience 2024Quote: ... were incubated for 2 h at room temperature (RT) with biotinylated polyclonal goat anti-human TREM2 capture antibody (0.1 μg/ml; AF1828, R&D Systems). Following incubation with streptavidin-HRP (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: The production of NO was measured using a colorimetric indirect method based on the detection of nitrite (nitrate was initially converted to nitrite by nitrate reductase) as a product of the Griess reaction (R&D Systems) in the supernatants of cell cultures ...
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bioRxiv - Biophysics 2022Quote: ... and human IL-13 (monoclonal capture antibody MAB213 and polyclonal detection antibody BAF213) were purchased from R&D Systems (Minneapolis, MN, USA).
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bioRxiv - Bioengineering 2024Quote: ... biotinylated affinity purified goat IgG anti-human interferon-γ detection antibody (Cat. #BAF285, lot #ZX2721071, R&D Systems, Minneapolis, Minnesota, United States), recombinant human interferon-γ protein (Cat ...
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bioRxiv - Molecular Biology 2021Quote: Retinal organoids on day 90 of differentiation were incubated at RT for 1 hour in PBS with either a goat anti-ACE2 antibody (AF933, R&D Systems) or a normal goat IgG control (AB-108-C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... pH 7.6] for 2 h at RT and incubated overnight (ON) at 4°C with antibodies against TRKB (1:1000, #AF1494, R&D Systems); AP2M (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... plates were incubated for 1.5 hr at RT with biotinylated sheep anti-mouse TREM2 antibody (0.1 µg/ml, BAF1729, R&D Systems, RRID: AB_356109) diluted in BSA-PBST ...
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bioRxiv - Immunology 2020Quote: ... were blocked using 5μg of mouse serum IgG for 10 min at RT then incubated with mouse anti-human ACE2 (R&D systems, FAB9333G), mouse antihuman CD81 (BD Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were blocked in PBS and Tween-20 with 1 % (w/v) nonfat dry milk for 1 h at RT followed by incubation with 1:50 PAX6 (AF8150; R&D Systems) or 1:500 FOXC1/2 (CPA3482 ...
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bioRxiv - Neuroscience 2023Quote: ... for 5 min each time and subsequently incubated with blocking buffer (5% goat serum [R&D Systems, Minneapolis ...
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bioRxiv - Immunology 2021Quote: Secreted IL-4 was measured using a matching antibody pair (capture, Tonbo 70-7041-U500; detection, eBio # 13-7042-85) with color generated using streptavidin-HRP (R&D Systems, Dx998) followed by Ultra-TMB reagent (Pierce ...
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bioRxiv - Immunology 2020Quote: ... were blocked using 1μg of mouse serum IgG for 10 min at RT then incubated with: AF-488 mouse anti-human ACE2 (R&D systems, FAB9333G), APC mouse antihuman CD81 (BD Biosciences ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in PBS and incubated for 30 minutes at room temperature (RT) with the human α-PlexinD1 mouse monoclonal antibody (1:150 dilution) (R&D systems, MAB4160). Cells were washed twice with PBS and incubated for 30 minutes at RT with an α-Mouse IgG secondary goat antibody conjugated to the APC fluorochrome (1:200 dilution ...
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bioRxiv - Neuroscience 2023Quote: Cortical neurons were treated with either 1.5 µM Aβ or rat recombinant TIMP-1 peptide (R&D systems, Cat# 580-RT-010, Minneapolis, USA) at 100 ng/ml32 or a combination of both for 16 h as shown in figures.
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bioRxiv - Immunology 2023Quote: Cytokines in the patient and control dermal fibroblast culture supernatants were measured using enzyme-linked immunosorbent assay (ELISA) for quantitative detection of IL-1β (R&D Systems Cat. No. DY201), tumour necrosis factor TNF-α (BioLegend® Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... Digested cells were washed three times with PBS and resuspended in 2/3 Cultrex Type 2 (R&D Systems) and 1/3 Full Ad-DF+++ medium and plated in drops which were allowed to solidify for 30 minutes at 37°C after which they were overlayed with Full Ad-DF+++ medium ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... plates were incubated for 2 h at RT with 500 ng/mL (25 ng/well) recombinant biotinylated human ACE-2 (R&D Systems, Cat# BT933-020) diluted in PBSTBA ...
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bioRxiv - Developmental Biology 2023Quote: ... blocked using 5% donkey serum or 5% BSA in PBST for 1 hour at RT and incubated overnight at 4°C with primary antibodies diluted in the blocking solution: CD31 (R&D systems; AF3628; 1:500), Aggrecan (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... and 10 µg/mL of mAb 18B7 in feeding medium using the Total NO and Nitrate/Nitrite Parameter (R&D Systems; detection limit, 0.78 μmol/L) and Griess Reagent method (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... Enzyme-Linked Immunosorbent Assay (ELISA) kit (DuoSet ELISA Ancillary Reagent Kit and Human Lubricin/PRG4 kit, R&D systems) was used following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... lymph node tissue sections derived from mice bearing pancreatic tumors were incubated overnight at RT with the following primary antibodies: NKp46 (R&D systems AF2225, used at 1:25), Ki67 (Abcam ab15580 ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed 3 times with wash buffer and 100 ul of samples and standards were added (R&D Systems, UK). Plates were left to incubate at room temperature for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... The plates were washed 3 times with washing buffer and a horseradish peroxidase-streptavidin (HRP-Strep) complex (R&D Systems DY998) diluted 1:25 in reagent diluent was added to the plates and incubated for 40 minutes with agitation ...
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bioRxiv - Molecular Biology 2022Quote: ... Subsequently plates were washed 3 times with PBS-T and primary antibody (anti-RBD-mouse, R&D systems, Minneapolis, MN, USA) was added in a dilution of 1 ...
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bioRxiv - Microbiology 2023Quote: Catalytic activities of SpyCEP constructs were measured through detection of remaining intact CXCL8 or CXCL1 substrate following incubation with SpyCEP by ELISA (R&D Systems human CXCL8 and CXCL1 DuoSet ELISA) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The expression of PD-L1 and IL-6 from bronchoalveolar lavages was quantitated following the protocols of ELISA kits: Human IL-6 Quantikine ELISA Kit and Human/Cynomolgus Monkey B7-H1/PD-L1 Quantikine ELISA Kit from R&D Systems.
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bioRxiv - Immunology 2023Quote: ... The following ELISA kits were used: TSLP mouse ELISA kit (EMTSLP; Thermo) and Mouse IL-33 Quantikine ELISA kit (M3300; R&D system). All samples were assessed in triplicate.
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... Next day the plates were washed 3 times and the color development was performed using Duoset ELISA Development System (DY999, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... fragments were washed three times in 5 ml cold AdDF+++ before being embedded in 30 μL BME (Type 2; R&D Systems) per well in a 48-well plate ...
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bioRxiv - Cancer Biology 2020Quote: ... Dot blots were washed with PBST three times and incubated with 30 μl of biotinylated anti-rabbit secondary antibody for 90 minutes (1:10 dilution, 865002, R&D Systems). After three PBST washes the dot blots were incubated with high sensitivity streptavidin conjugated to horseradish peroxidase (1:10 dilution ...
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bioRxiv - Cancer Biology 2021Quote: SUM159-LM1 cells or MDA231-LM2 cells were suspended in HuMEC/0.1% BSA and treated with EC-derived conditioned media (CM) (diluted 2-5 times in the final medium) or 50 ng/ml of recombinant activin B (R&D systems). 2,500 cells/well of SUM159-LM1 cells or 5,000 cells/well of MDA231-LM2 cells were seeded on 96-well ultra-low attachment plate (Corning) ...
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bioRxiv - Immunology 2023Quote: Culture media of cells infected with virus were collected at the indicated times and used for ELISA quantification of IFNβ (R&D Systems) in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed three times with 2% PBS-BSA and incubated with secondary anti-goat IgG (H+L) APC-conjugated antibody (F0108, R&D systems) in 2% PBS-BSA for 20 minutes at room temperature at 0.05 µg/106 cells for ACE2-A549 cells or at 0.1 µg/106 cells for Vero E6 cells ...
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bioRxiv - Developmental Biology 2023Quote: E13.5 small intestinal tissues were harvested in Calcium/Magnesium free (CMF) PBS and washed several times in CMF PBS before being placed in 3mL of Cultrex Organoid Harvesting Solution (R&D Systems), a non-enzymatic basement membrane depolymerization solution ...
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bioRxiv - Immunology 2021Quote: ... and DuoSet Human IFN-gamma kit and Ancillary Reagent Kit 2 (R&D Systems) were performed according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... and incubated with antibodies against Kit (1:200 anti Kit AF1356 R&D Systems). The antigen-bound primary antibodies were detected using 1:500 dilution of biotinylated anti-goat IgG antibody (BA-5000-1.5 ...