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Citations for R&D Systems :
1 - 50 of 1186 citations for Yeast Expression Cloning kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 50 μM recombinant yeast ubiquitin (R&D Systems), 5 μM Rad6 or Rad6-150 ...
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bioRxiv - Biophysics 2023Quote: ... and either 100 μM yeast ubiquitin (R&D Systems) or 100 μM ubiquitinCy3 ...
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bioRxiv - Molecular Biology 2021Quote: ... 2.5 µM recombinant yeast or human ubiquitin (R&D Systems), 0.1 µM wild-type or mutant Rad6 or UBE2B (E2) ...
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bioRxiv - Biochemistry 2023Quote: ... 125 μM ubiquitin (yeast recombinant from bio-techne (R&D Systems)) ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 mM ATP) with 0.1 μM recombinant yeast E1 (R&D Systems), ...
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bioRxiv - Bioengineering 2023Quote: ... Expression levels of murine vascular endothelial growth factor (VEGF) (Quantikine ELISA kit, R&D Systems, Minneapolis, MN, USA) and HIF-1α (SimpleStep ELISA kit ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.1 µM recombinant yeast or human GST-Uba1/UBE1 (E1, R&D Systems), 2.5 µM recombinant yeast or human ubiquitin (R&D Systems) ...
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bioRxiv - Microbiology 2020Quote: Expression of cytokines/chemokines in lung homogenates was assessed by ELISA using DuoSet kits (R&D Systems, Minneapolis, MN) for growth-regulated alpha protein (CXCL1/KC) ...
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bioRxiv - Pathology 2020Quote: ... kidney samples were processed for TNF-α expression using the Mouse TNFα Quantikine ELISA Kit (R&D Systems MTA00B). Samples were diluted 1:5 in reagent diluent prior to plating ...
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bioRxiv - Immunology 2023Quote: Phosphoprotein expression of CD8+ T cells was detected using a human phospho-kinase array kit (ARY003C, R&D Systems, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Expression of angiogenic proteins in CM was measured using a Proteome Profiler Human Angiogenesis Array kit (ARY007, R&D systems) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The expression of angiogenesis-related proteins was detected by using the Proteome Profiler Mouse Angiogenesis Array Kit (ARY015; R&D Systems) following the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2020Quote: Phosphorylation of several kinases and expression levels of matrix metallopeptidases (MMPs) were analyzed using Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems) and Human MMP Antibody Array Kit (Abcam) ...
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bioRxiv - Microbiology 2023Quote: ... cell-free supernatant was collected and a semiquantitative immunosorbent assessment of cytokine expression was performed using a Proteome Profiler Human Cytokine Array Kit (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Relative expression level of 105 soluble human proteins and cytokines were determined using the Human XL Cytokine Array Kit from R&D Systems. The cytokine array was performed following the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2024Quote: ... Relative expression levels of 105 soluble human proteins and cytokines were determined using the Human XL Cytokine Array Kit from R&D Systems. The cytokine array was performed following the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2021Quote: ... The relative expression profile of 36 human cytokines and chemokines were evaluated using the Proteome Profiler Human Cytokine Array Kit (R&D Systems, ON, Canada). The experiment was performed according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Intracellular cAMP expression levels were measured after 10min to 48h of PTH treatment using Mouse/Rat cAMP Assay Parameter™ Kit (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions in both pre-differentiation COBs and BMSCs ...
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bioRxiv - Bioengineering 2023Quote: ... The digested samples were centrifuged and only the top supernatant was used in the quantification of expression levels of murine VEGF (Quantikine ELISA kit, R&D Systems, Minneapolis, MN, USA) and HIF-1α (SimpleStep ELISA kit ...
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bioRxiv - Cancer Biology 2020Quote: ... and analyzed for expression of using either MMP9 (R&D Systems AF911). To detect expression of β-Actin clone AC-15 (Catalog Number A5441 ...
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bioRxiv - Immunology 2023Quote: ... and IFNGR2 expression (Hamster Anti-Mouse IFNGR2 Monoclonal antibody; MAB773, R&D systems) by flow cytometry ...
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bioRxiv - Immunology 2022Quote: ... Media was harvested and investigated for expression of IL-17 by ELISA (R&D systems).
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bioRxiv - Bioengineering 2020Quote: ... IL-21 expression was measured using a human IL-21 DuoSet ELISA (R&D systems).
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bioRxiv - Immunology 2022Quote: ... After 18 hours media was harvested and investigated for expression of CCL20 by ELISA (R&D systems). Interleukin-2 expanded MAIT cells (1×106 ...
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bioRxiv - Bioengineering 2021Quote: ... GDF-15 expression was analyzed using Human GDF-15 DuoSet® ELISA (DY957; R&D Systems™). For the activation of latent TGF-β1 ...
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bioRxiv - Biochemistry 2022Quote: ... We verified expression and purification by immunoblotting with an anti-LECT2 antibody (#MAB722, R&D Systems, MN, USA). Cells were grown in terrific broth media at 37 °C ...
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bioRxiv - Pathology 2024Quote: ... Cell surface protein expression was performed by labeling 2×105 cells for KDR-PE (R&D Systems, FAB357P) + PDGFRα-APC (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: ... NKG2DLs expression in each cancer cell line was stained with recombinant human NKG2D Fc chimera (R&D Systems, USA) and then FITC-conjugated anti-human Fc secondary antibody was used as a detection antibody (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... The expression of EGFRt was determined using an anti-EGFR-FITC antibody (R&D Systems, Minneapolis, MN, USA, FAB10951G) or GFP expression analyzed by Cytoflex Flow Cytometer (Beckman Coulter ...
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bioRxiv - Immunology 2019Quote: Relative expression levels of 111 plasma cytokines were quantified by the Proteome Profiler Mouse XL Cytokine Array (R&D Systems). The antibody array was performed according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... Expression of the adipokines were analyzed by Quick spots software designed for proteome arrays (R&D Systems, Minneapolis, MN, USA).
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bioRxiv - Bioengineering 2020Quote: Expression levels of cell secreted Osteoprotegerin and Vascular Endothelial Growth Factor (VEGF) was examined via ELISA (R&D Systems, Minnesota, USA). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... the cell populations with a higher ACE2 expression in clone 43.20 were FACS sorted using ACE2-specific antibody (AF933, R&D Systems). After puromycin selection ...
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bioRxiv - Developmental Biology 2021Quote: ... Clones were screened for ACVR1 or ALK3 protein expression by flow cytometry with an anti-ACVR1 antibody (R&D Systems #AF637) and an anti-ALK3 antibody (Sino Biological #50078) ...
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bioRxiv - Cell Biology 2023Quote: Surface expression of anti-EGFR CARs was detected by staining with an anti-Cetuximab-AF647 idiotype (Clone: 2259C, R&D Systems). T cell editing efficiency ...
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bioRxiv - Developmental Biology 2023Quote: Surface expression of ITFG1 on cells was examined by staining the cells with mouse anti-ITFG1 antibody (R&D Systems, MAB89001) at 4°C for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... accurate ACE2 expression was controlled on western blot probed with anti-ACE2 antibody (Human ACE-2 Antibody, AF933, R&D systems). ACE2-positive cells (70-80% confluence ...
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bioRxiv - Biochemistry 2020Quote: ... All cell lines were routinely evaluated on a Flow Cytometer (Becton Dickinson, FACScan) for the expression of CD marker using anti-hCD3ε (PE Conjugated Mouse IgG1, R&D Systems) antibody to authenticate the cell line.
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bioRxiv - Microbiology 2021Quote: ... The surface expression of hACE2 was confirmed by flow cytometry using anti-human ACE2 AlexaFluor 647-conjugated Ab (R&D Systems, # FAB9332R). The expression was further confirmed by immunoblot using hACE2 Ab (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2024Quote: ... GPC2 CAR expression was determined using APC or PE-conjugated human recombinant GPC2 protein (R&D Systems; 2304-GP-050; 1:500) and CD19 CAR using PE-tagged protein L (58036S ...
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CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... Receptor surface expression levels were verified by flow cytometry using CXCR4- and CXCR7-specific mAbs (12G5, R&D Systems and 8F11-M16, BioLegend) on a BD FACS Fortessa cytometer (BD Biosciences).
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bioRxiv - Immunology 2021Quote: The expression of NKG2D ligands in tumor cell lines was assessed using Recombinant Human NKG2D Fc Chimera Protein (R&D systems, Abingdon, UK). 105 tumor cells were incubated either with 0.5 μg of NKG2D Fc recombinant protein or IgG1-Fc during 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were left for 48h to allow expression of LepRb-HA protein before treatment with recombinant murine leptin (R&D System #498-OB) at 0-10 ng/L for 30 minutes before cell lysis and western blotting for phosphoSTAT3 (Y705) ...
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bioRxiv - Cell Biology 2022Quote: Expression levels of TGFA and VEGF-A in cell culture supernatants were measured using Human Quantikine ELISAs (R&D Systems, Minneapolis, MN, USA) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... POSTN protein expression in the medium was confirmed using an ELISA assay with an anti-POSTN polyclonal antibody (R&D Systems, Cat # AF3548). 3T3-CTL cells were used to generate “control” conditioned medium (CMCTL) ...
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bioRxiv - Genetics 2020Quote: MEFs cultured in collagen-coated six-well plates were transiently transfected with indicated expression vectors and stimulated overnight with100 nM PMA (R&D Systems, Minneapolis, USA). Cell culture supernatant was centrifuged at 2500xg at 4°C for 5 min and 100 uls of supernatant was used to measure AREG protein levels by a Mouse Amphiregulin DuoSet ELISA Developmental Kit (#DY989 ...
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bioRxiv - Microbiology 2020Quote: ... Ad-transduced cells were incubated for 24h before performing flow cytometry staining for surface expression of hACE2 using goat anti-human mAb AF933 (R&D Systems, Minneapolis, MN) used at a final concentration of 10μg/mL ...
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bioRxiv - Biochemistry 2024Quote: ... Confirmation of ETB gene expression was confirmed in cell populations by surface staining with antibodies (anti-HA tag AlexaFluor 488 conjugated mouse IgG, R&D Systems, cat # IC6875G) in combination with flow cytometry ...
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bioRxiv - Developmental Biology 2023Quote: Flow cytometry performed within 1-6 days investigated NOTCH1 expression using anti-NECD1 antibody AF5267 and Notch ligand binding for DLL1-Fc (R&D Systems, Inc., Minneapolis, MN) and DLL4-Fc (Aro Biosystems ...
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bioRxiv - Bioengineering 2022Quote: ... Enzyme-Linked Immunosorbent Assay (ELISA) kit (DuoSet ELISA Ancillary Reagent Kit and Human Lubricin/PRG4 kit, R&D systems) was used following manufacturer’s protocol ...