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Citations for R&D Systems :
201 - 250 of 290 citations for U Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2019Quote: ... cells were washed with 1× Phosphate buffered saline (PBS) and stimulated with 1000 U/mL of human recombinant IFNβ (R&D Systems) in DMEM for the designated length of time ...
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bioRxiv - Immunology 2020Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Immunology 2020Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Immunology 2020Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Immunology 2022Quote: ... T cells were stimulated for 48 hours at 1e6 cells per ml of media containing 500 U/ml IL-2 (R&D Systems), 5 ng/ml IL-7 (R&D Systems) ...
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bioRxiv - Immunology 2022Quote: ... The single memory B cells were cultured in complete RPMI 1640 medium supplemented with IL-2 (10 U/ml, R&D Systems), IL-21 (10 U/ml ...
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bioRxiv - Immunology 2021Quote: ... wells were coated with 30 μg/mL PD-L1-Fc chimera protein (R&D Systems) or 6 μg/mL anti-ICOS (clone C398.4A ...
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bioRxiv - Cell Biology 2020Quote: ... the wells were added with anti-MTf antibody (50 ng of MAB81751, R&D Systems) in PBS-5% BSA for 2 h at RT ...
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bioRxiv - Immunology 2022Quote: ... 50 μL/well of a 1:1 solution of H2O2 (R&D Systems, part #895000) and tetramethylbenzidine (R&D Systems ...
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bioRxiv - Bioengineering 2023Quote: ... as well as the Human Mesenchymal Stem Cells Multi-Color Flow Kit (R&D Systems) including antibodies for positive markers CD44 ...
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bioRxiv - Cell Biology 2023Quote: ... all the wells were treated with TGFb1 (R&D systems, #240-B-010, 5ng/ml). Drugs were used at a concentration of 10µM ...
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bioRxiv - Cancer Biology 2024Quote: ... at a 1:10 ratio and plated on 2-well comet slides (R&D Systems). Cells were then lysed overnight and immersed in alkaline unwinding solution as per manufacturer’s protocol (Trevigen) ...
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bioRxiv - Bioengineering 2020Quote: Cytokine and chemokine levels were measured in the basal outflow of mouse intestine chips using a custom MSD U-plex Assay (Meso Scale Diognostics) and the mouse CXCL1/KC DuoSet ELISA (R&D Systems DY453) according to the manufacturers’ instructions.
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bioRxiv - Genomics 2019Quote: ... as from previous dose-response experiments3–5: recombinant human IL-1β (specific activity 1.8×107 U/mg; 201-LB-005, R&D Systems, Abingdon, UK) at 50 U/ml ...
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bioRxiv - Immunology 2020Quote: ... Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems; R15-100). R15-100 was added every 2 to 3 days thereafter ...
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bioRxiv - Immunology 2023Quote: ... and the reaction was stopped by adding 0.5N HCl per well (both from R&D Systems). Samples were analyzed at 450nm using the Benchmark Plus microplate spectrophotometer (Bio-Rad).
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bioRxiv - Immunology 2021Quote: ... the bone marrow cells were culture in 6-well plates at density of 3×106 cells/mL in 3mL DC medium in the presence of mouse granulocyte macrophage-colony stimulating factor (GM-CSF; 1000 U/mL; R&D Systems, Minneapolis, MN) and interleukin 4 (IL-4 ...
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bioRxiv - Microbiology 2023Quote: ... 1 x 107 cells were added to 100mm Petri dishes containing 15 ml of complete RPMI media plus 200 to 1000 U/ml IFN-β (R&D Systems 8234MB010). All cells were grown in a 37⁰C incubator with 7.5% CO2 for 5 h to 4 days (as indicated ...
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bioRxiv - Cell Biology 2021Quote: ... Pro-inflammatory stress was induced using a cytokines mix composed of: IL-1β (50 U/mL) (cat. 201-LB-005, R&D system Minneapolis, MN, USA), TNFα (1000 U/mL ...
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bioRxiv - Immunology 2024Quote: ... were grown in DMEM supplemented with 10% FBS and 100 U/ml penicillin–streptomycin in the presence of macrophage colony-stimulating factor (M-CSF, 10 ng/ml, R&D Systems 416-ML-050), as described earlier in our studies ...
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bioRxiv - Immunology 2022Quote: ... The reaction was stopped by the addition of 50μL/well of 2N H2SO4 (#DY994, R&D Systems), and the OD of the samples were measured at 450 nm (Epoch Microplate Spectrophotometer ...
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bioRxiv - Pathology 2020Quote: ... An anti-TLR4 capture antibody (100 ng/well in carbonate-bicarbonate buffer; R&D Systems, Minneapolis, MN) with demonstrable specificity for TLR in vitro (see Western blot ...
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bioRxiv - Immunology 2019Quote: ... as well as ESAT-6 tetramer (as above) presence of 1μg/ml Cyclosporine A (R&D Systems) to prevent tetramer-mediated activation of T cells ...
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bioRxiv - Pathology 2021Quote: A total of 50 μL/well of recombinant human IL5-Receptor alpha (R&D Systems, Minneapolis, MN) final concentration of (4 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... and all steps were followed by washing using 200 μL/well of wash buffer (R&D Systems) three times ...
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bioRxiv - Neuroscience 2023Quote: ... coated 6 well plates in oRIM supplemented with 2.6 nM (20 ng/mL) IGF1 (R&D Systems). On day 24 ...
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bioRxiv - Microbiology 2023Quote: ... ligand-loaded CD1d (0.25 ug per well) was bound onto Streptavidin-coated plates (R&D Systems #CP003). Plates were washed with PBS three times and then added 24.7 NKT cells (4 x 104∼105 per well ...
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bioRxiv - Immunology 2021Quote: ... Next day wells were washed and incubated with Streptavidin-AP (R&D systems, mouse IFNγ Kit Cat # EL485) for 2h at RT ...
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bioRxiv - Genomics 2019Quote: ... A control well containing 480 ng/ml of neutratlizing anti-LIF antibody (R&D Systems AB-449-NA) was also plated ...
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bioRxiv - Biochemistry 2021Quote: ... Test wells were coated with 5 µg recombinant spike protein (rSP; S1+S2; R&D Systems, Minneapolis, MN)/ml coating buffer (0.05 M sodium bicarbonate ...
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bioRxiv - Developmental Biology 2021Quote: ... the wells were plated with 25µl detection antibody (Biotinylated Goat Anti-Mouse IGF-II, R&D Systems – 840963), diluted to 0.72 µg/ml in PBS ...
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bioRxiv - Immunology 2021Quote: ... were seeded on 6 well plate and cultured with recombinant mouse IFNγ (100 units/mL; R&D Systems) and recombinant human IL-1β (5 units/mL ...
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bioRxiv - Immunology 2022Quote: ... were coated with 100 μl per well of 1.5 μg/ml human recombinant integrin αvβ6 (R&D Systems) diluted in coating buffer from the ELISA Starter Accessory Kit (Bethyl Laboratories ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The wells were washed extensively and HRP-conjugated goat anti-rabbit secondary antibody (R&D Systems, Minneapolis, MN) was added to incubate for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... Subsequently the cells were rested and expanded in kool aid complete media for 2 days supplemented with 100 U/mL recombinant Human IL-2 (R&D Systems Cat# 202-IL-010/CF). Subsequently ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.03 to 3 μg/well of the soluble mouse or human HA-tagged recombinant fibulin 7 (R&D Systems) in 2% dry milk/TBS and 2 mM CaCl2 were added to the wells and incubated for 3 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of 2,000 cells/well were resuspended in collected CM and 10 ng/mL rhFGF (R&D Systems), 10 ng/mL hEGF (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... ~70,000 cells/well were seeded in a 8-chamber slide coated with either 10μg/mL rNPNT (R&D system), 2μg/mL BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... Monocytes were plated at 107 cells/well and differentiated with Dendritic Cell Differentiation Kit (cat# CDK004, R&D Systems) according to the manufactures instructions ...
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Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cellsbioRxiv - Developmental Biology 2020Quote: ... at 3500 cells per well in GK15 medium supplemented with 200 ng/ml BMP4 (R&D Systems, 314-BP-010), 100 ng/ml SCF (R&D Systems ...
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bioRxiv - Immunology 2020Quote: ... culture supernatants were analyzed for antibody production as well as IL-2 and IFNγ secretion by ELISA (R&D Systems). In CD4 blocking expriments ...
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bioRxiv - Immunology 2020Quote: ... the binding was detected using 3,3’,5,5’-Tetramethylbenzidine (TMB) substrate (100 μl/well) (DuoSet ELISA Ancillary Reagent Kit, R&D Systems) as per the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2023Quote: ... 0.5% bovine serum albumin (BSA)) and labeled with 0.02 µg/well un-conjugated anti-ACKR3 antibody (11G8, R&D Systems) for 1 hour at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Wells were flooded with BrainPhys media with supplements and 10 ng/mL human NGF (256-GF-100, R&D Systems), and 3 μg/mL FRDU and incubated at 37° C and 5% CO2 until further experimentation.
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bioRxiv - Immunology 2021Quote: ... After stimulation wells were washed and incubated with a biotinylated anti-IFNγ antibody (R&D systems, mouse IFNγ Kit Cat # EL485) overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Nunc® Maxisorp™ 384-well plates were coated with 6 µg/ml of recombinant mouse pIgR protein (R&D Systems) diluted in coating solution concentrate (Seracare ...
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bioRxiv - Cell Biology 2021Quote: ... limiting dilutions of LSK cells and their respective replicate wells were subjected to methylcellulose colony assay with HSC007 (R&D Systems) at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: Cells were seeded in 12-well plates and treated for 8 hours in biological quadruplicates with IFNα (0.5 ng/ml, R&D Systems), IFNβ (0.5 ng/ml ...
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Molecular architecture determines brain delivery of a transferrin-receptor targeted lysosomal enzymebioRxiv - Neuroscience 2021Quote: ... A 384-well maxisorp plate (Thermo, Cat# 464718) was coated overnight with an anti-IDS antibody (R&D Systems, Cat# AF2449) and blocked with Casein-PBS Buffer (Thermo ...
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bioRxiv - Microbiology 2019Quote: ... Mouse and human CXCL1 and CXCL2 as well as human IL-8 (CXCL8) were obtained from R&D Systems (Minneapolis, MN) and multiplex CXCL1 ...