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Citations for R&D Systems :
301 - 350 of 2039 citations for TNF alpha Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Transiently transfected Jurkat cells in a volume of 500 μL were incubated in the absence or presence of the activators: TNF-α (20 ng/mL; R&D Systems, Minneapolis, MN), Phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Biophysics 2023Quote: ... A “type-1” like cytokine cocktail consisted of 10 ng/ml of recombinant mouse tumor necrosis factor-α (TNF-α, R&D Systems, Minneapolis, MN), 5 ng/ml of recombinant mouse interleukin-1β (IL-1β ...
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bioRxiv - Bioengineering 2023Quote: ... Conditioned media was collected 24 hours later and stored at -80 °C until analysis of TNF-⍰ levels via ELISA (DY210; R&D Systems, Minneapolis, MN, USA).
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bioRxiv - Immunology 2023Quote: The concentrations of IL-13 and TNF-α in the culture media were measured by using mouse IL-13 DuoSet ELISA (R&D Systems, Minneapolis, MN) and ELISA MAX Deluxe Set mouse TNF-α (BioLegend) ...
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bioRxiv - Pathology 2023Quote: ... CXCL2, CXCL1, TNF, MMP8, S100A8, IFN-γ, IL12p40, IL-12p70, IL-10, and VEGF using antibody pairs and standards from R&D Systems (Minneapolis, MN), Invitrogen (Carlsbad ...
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Salinomycin inhibits epigenetic modulator EZH2 to enhance Death Receptors in Colon Cancer Stem CellsbioRxiv - Cancer Biology 2020Quote: ... Proteome profiler Human Apoptosis array kit and recombinant human TRAIL were obtained from R&D Systems. Primers for GAPDH ...
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bioRxiv - Pathology 2021Quote: ... Human MPCs were trypsinized and incubated 30 min with biotinylated anti-human PDGFRα (R&D system) and CD56-PE (clone B159 ...
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bioRxiv - Cell Biology 2021Quote: Human recombinant EGF (236-EG) and human transferrin (2474-TR) were purchased from R&D Systems. Alexa Fluor 647-conjugated EGF (E35351) ...
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bioRxiv - Immunology 2021Quote: ... Recombinant human TGF-β1 and mouse or human IL-2 were purchased from R&D system or Biolegend ...
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bioRxiv - Cell Biology 2024Quote: KRT20-tdTomato human colon organoids were treated with recombinant human IL-22 protein (R&D Systems) from day 4 to day 7 of organoid cultures in normal organoid media without WNT and R-Spondin ...
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bioRxiv - Cell Biology 2022Quote: Human recombinant JAG1 (R&D System) was fluorescently labeled with Alexa Fluor™ 488 Microscale Protein Labeling Kit (Thermo Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... Fgfr2iiib-Fc (human, R&D Systems) and Phospho-p44/42 MAPK (rabbit ...
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bioRxiv - Immunology 2019Quote: ... human IL-18 (R&D Systems) and CXCL8 (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... recombinant human IFNγ (R&D Systems) was also added at varying concentrations ...
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bioRxiv - Cancer Biology 2020Quote: Recombinant human GDF15 (R&D Systems, Minneapolis ...
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bioRxiv - Physiology 2021Quote: ... and human (DANG10, R&D systems) protein levels according to the manufacturer’s recommendations.
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bioRxiv - Neuroscience 2020Quote: ... and human GFRα1 (R&D Systems) for 4 h at a final concentration of 100 ng/ml ...
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bioRxiv - Developmental Biology 2019Quote: ... recombinant human BMP4 (R&D Systems) or CHIR-99201 (Wako) ...
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bioRxiv - Bioengineering 2019Quote: ... recombinant human Wnt7a (R&D systems) reconstituted in PBS was added to the PEG-4MAL precursor solution ...
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bioRxiv - Immunology 2022Quote: ... Recombinant human MBL (R&D systems) was reconstituted in PBS and serially diluted from 10μg/ml by two-fold dilution ...
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bioRxiv - Physiology 2022Quote: ... Recombinant human WNT3A (R&D Systems) and recombinant human Norrin (R&D Systems ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant human BMP4 (R&D Systems) was added at 1.5 nM (55 ng/ml)7 ...
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bioRxiv - Neuroscience 2023Quote: ... and S100B (Human: R&D Systems, 1820-SB ...
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bioRxiv - Immunology 2024Quote: ... Each human FcγR (R&D Systems) was captured on immobilized anti-His antibodies at 100-200 RU level ...
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bioRxiv - Bioengineering 2024Quote: ... Recombinant human VEGF (R&D Systems) was added to the basal media at 20 ng/mL immediately prior to cell treatment ...
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bioRxiv - Developmental Biology 2021Quote: The following antibodies were used for immunofluorescence: goat anti-platelet derived growth factor-alpha (PDGFRA, 1:1000, AF1062, R&D Systems), rat anti-PDGFRA (PDGFRA ...
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bioRxiv - Cell Biology 2021Quote: ... which was constructed using the cDNA sequences of mouse IL-6/IL-6R alpha fusion protein (9038-SR, R&D systems). AAV8-Null vector was used as vector control.
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bioRxiv - Immunology 2022Quote: ... 6.25 ng pMHC and 6.25 ng recombinant mouse PD-L1 with 6-His tag (R&D systems) in 2% BSA were injected into each well in this step ...
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bioRxiv - Developmental Biology 2019Quote: ... were incubated with mouse FLT1-FC chimeric protein with His-tag (471-F1-100, R&D Systems). Plates were washed with 0.05% Tween 20/PBS ...
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bioRxiv - Immunology 2023Quote: ELISA plates were coated with 2 µg/ml mouse anti-His tag antibody (R&D Systems, MAB050) and blocked as described above ...
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bioRxiv - Biophysics 2024Quote: ... were functionalized with biotinylated α-penta-His antibody (Qiagen, Hilden Germany or R&D Systems, Minneapolis MN) and stored in BRB80 containing 8 mg/ml BSA and 1 mM DTT at 4 °C with continuous rotation ...
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bioRxiv - Neuroscience 2019Quote: ... The TNF-α concentration in the supernatant was quantified using the Duoset® enzyme-linked immunosorbent assay (ELISA) development system (R&D Systems, Abingdon, Oxfordshire, UK). Three wells for each CSF were used to estimate variation in the experiments ...
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bioRxiv - Cell Biology 2020Quote: Supernatant of confluent pulmonary endothelial cells were analyzed for TNF-α and IL-6 by using a mouse-specific ELISA from R&D Systems (Minneapolis, MN, USA) according to manufacturer’s protocol with the help of an Infinite M200 PRO plate reader (TECAN Instruments ...
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bioRxiv - Immunology 2019Quote: ... Levels of IL-1β and TNF-α in 200 μl culture medium were measured by ELISA kits according to manufacturer’s instructions (DY401 for IL-1 β and DY410 for TNF-α, R&D systems, Minneapolis, MN, USA). Cleavage of pro-IL-1β and pro-caspase-1 was assessed by western blot of 50 μg of protein extracts of cell lysates ...
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bioRxiv - Immunology 2022Quote: IL-10 and TNF-α levels were measured in the cell-free supernatants of cell cultures using a cytokine Duo-Set Kit (R&D Systems, Minneapolis, MI, USA), according the manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... supplemented with 2% fetal bovine serum for 6 hours and then left untreated or treated with 10 ng/ml IL-17A or 20 ng/ml IL-36γ alone or with the addition of 10 ng/ml TNF-α (R&D Systems, Minneapolis, MN, USA) for 24 hours ...
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bioRxiv - Cell Biology 2024Quote: ... co-housed 8-week-old female C57BL/6J wildtype mice were administered either 300 μg/kg TNF (R&D Systems Cat#410-MT/CF Lot CS152103) in endotoxin-free DPBS or endotoxin-free DPBS alone (Merck Cat# TMS-012-A ...
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bioRxiv - Cell Biology 2024Quote: ... TNF-α and IL-6 release was measured using DuoSet ELISA kits according to the manufacturer’s instructions (R&D Systems: DY417-05; DY410-05; DY406-05).
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bioRxiv - Immunology 2022Quote: ... Human ACE2 was detected using a polyclonal goat anti-human ACE2 antibody (1:500, R&D Systems), a horseradish peroxidase (HRP)-labeled donkey anti-goat antibody (1:5000 ...
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bioRxiv - Physiology 2021Quote: ... Human MPCs were trypsinized and incubated 30 min with biotinylated anti-human PDGFRα (BAF322 R&D Systems) goat polyclonal antibody and CD56-PE (clone B159 ...
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bioRxiv - Cell Biology 2021Quote: Recombinant human DLL4 (DLL4 fused with the Fc domain of human IgG; R&D Systems; 1506-D4) was resuspended to a final concentration of 10 μg/ml in sterile PBS containing 1% wt/vol bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... and human serum osteopontin was measured using the Human Osteopontin DuoSet ELISA kit (R&D Systems Inc.). Phosphate homeostasis hormones were measured in mouse and human serum using the FGF-23 ELISA Kit (Kainos Laboratories Inc. ...
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bioRxiv - Immunology 2023Quote: Quantification of REG3A in human stool extracts was performed using Human Reg3A DuoSet ELISA (R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: The human ADGF (Recombinant Human Adenosine deaminase 2/CECR1) #7518-AD-010 was from R&D Systems, Minneapolis ...
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bioRxiv - Neuroscience 2019Quote: ... for 24h or with 10 ng/mL tumor necrosis factor alpha and 10ng/ml IL1-ß(R&D Systems; 401-ML-005) for 24h ...
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bioRxiv - Genomics 2022Quote: Three million cells from each triplicate were seeded separately in 6 well-plates in fresh RPMI media and left unstimulated or stimulated with universal interferon alpha (200U/ml) (R&D systems) for 24 hours ...
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bioRxiv - Immunology 2022Quote: ... Urine albumin and creatinine were measured using ELISA kits (Alpha Diagnostic International, San Antonio, USA, and R&D systems, Minneapolis, USA, respectively). Urine samples were diluted to fit within the standards range of each kit ...
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bioRxiv - Cell Biology 2023Quote: ... Cytokine levels in BMEF were then measured using mouse CXCL12/SDF-1 alpha (MCX120) and SCF (MCK00) Quantikine ELISA kits (R&D Systems) according to the manufacturer’s protocols.
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bioRxiv - Neuroscience 2022Quote: ... TNF-α (MTA00B) and IL-6 (M6000B) levels were determined using enzyme-linked immunosorbent assay (ELISA) kits (ELISA Development System; R&D Systems, McKinley Place, MN, USA) following the manufacturer’s protocol.75
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bioRxiv - Cell Biology 2020Quote: 0.3M of purified proteins (recombinant human E-cadherin 8505 and recombinant human EGFR 344-ER R&D system) were mixed and incubated in 100μl of lysis buffer (25 mM TrisHCl pH 7.5 ...